BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0200.p (169 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001353035.1| riboflavin synthase subunit beta [Janthinoba... 241 2e-62 ref|YP_001100354.1| riboflavin synthase subunit beta [Herminiimo... 227 5e-58 ref|YP_003775379.1| 6,7-dimethyl-8-ribityllumazine synthase [Her... 225 2e-57 ref|YP_004754714.1| 6,7-dimethyl-8-ribityllumazine synthase [Col... 224 4e-57 ref|YP_004355724.1| riboflavin synthase [Pseudomonas brassicacea... 222 1e-56 >ref|YP_001353035.1| riboflavin synthase subunit beta [Janthinobacterium sp. Marseille] gb|ABR88799.1| riboflavin synthase beta chain [Janthinobacterium sp. Marseille] Length = 167 Score = 241 bits (615), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 113/159 (71%), Positives = 133/159 (83%), Gaps = 1/159 (0%) Query: 1 MQHPSS-NTSSARNRIAFIQASWHKEIVDQCRISFTAEIVKLGFAESSIDFFELPGAFEI 59 MQHP T SA RIAFIQASWHKEIVDQCRI+F AE+ + G+ E SIDFFE+ GAFEI Sbjct: 1 MQHPIDIKTKSAVRRIAFIQASWHKEIVDQCRIAFIAEMAERGYTEDSIDFFEVAGAFEI 60 Query: 60 PLHAKLLAKTGKYAGIVAAGLVVDGGIYRHDFVAQAVITSLMNVQLETEVPVFSAVLTPH 119 PLHAK+LAK+G+YA +V GLVVDGG+YRH+FV+QAVI+ LM VQLETEVPV SAVLTPH Sbjct: 61 PLHAKVLAKSGRYAAVVGTGLVVDGGVYRHEFVSQAVISGLMQVQLETEVPVISAVLTPH 120 Query: 120 HFHSHGEHQQFFQEHFVVKGQEVARACADTVVKVARIEA 158 HFHSH EHQ FF +HF+VKG+E A ACADT+ ++ +IEA Sbjct: 121 HFHSHDEHQNFFFQHFLVKGKEAAHACADTIARLQKIEA 159 >ref|YP_001100354.1| riboflavin synthase subunit beta [Herminiimonas arsenicoxydans] emb|CAL62231.1| 6,7-dimethyl-8-ribityllumazine synthase 2 (DMRL synthase 2) (Lumazine synthase 2) (Riboflavin synthase 2 beta chain) [Herminiimonas arsenicoxydans] Length = 167 Score = 227 bits (578), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 108/159 (67%), Positives = 128/159 (80%), Gaps = 1/159 (0%) Query: 1 MQHPSS-NTSSARNRIAFIQASWHKEIVDQCRISFTAEIVKLGFAESSIDFFELPGAFEI 59 MQH SA RIAFIQASWHKEIVDQCRI+F E+ G++ IDFFE+ GAFEI Sbjct: 1 MQHAIDIKAKSAARRIAFIQASWHKEIVDQCRIAFVEEMAARGYSADVIDFFEVAGAFEI 60 Query: 60 PLHAKLLAKTGKYAGIVAAGLVVDGGIYRHDFVAQAVITSLMNVQLETEVPVFSAVLTPH 119 PLHAK+LAK+G+YA IV GLVVDGG+YRH+FV+QAVI+ LM VQLETEVPV SAVLTPH Sbjct: 61 PLHAKVLAKSGRYAAIVGTGLVVDGGVYRHEFVSQAVISGLMQVQLETEVPVISAVLTPH 120 Query: 120 HFHSHGEHQQFFQEHFVVKGQEVARACADTVVKVARIEA 158 HFHSH EHQ FF +HF+VKG+E A ACADT+ ++ +I+A Sbjct: 121 HFHSHDEHQGFFFQHFLVKGKEAAHACADTIQRLQQIDA 159 >ref|YP_003775379.1| 6,7-dimethyl-8-ribityllumazine synthase [Herbaspirillum seropedicae SmR1] gb|ADJ63471.1| 6,7-dimethyl-8-ribityllumazine synthase (riboflavin synthase beta chain) protein [Herbaspirillum seropedicae SmR1] Length = 169 Score = 225 bits (573), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 120/167 (71%), Positives = 142/167 (85%), Gaps = 2/167 (1%) Query: 3 HPSSNTSSARNRIAFIQASWHKEIVDQCRISFTAEIVKLGFAESSIDFFELPGAFEIPLH 62 H S+ +S RIAFIQA WHK+IVDQCRI+FT E+ KLG+ +SIDFFELPGAFEIPLH Sbjct: 5 HLSAVSSHHGQRIAFIQACWHKDIVDQCRIAFTEEMAKLGWPAASIDFFELPGAFEIPLH 64 Query: 63 AKLLAKTGKYAGIVAAGLVVDGGIYRHDFVAQAVITSLMNVQLETEVPVFSAVLTPHHFH 122 AK+LAKTG+YAGIVA+GLVVDGGIYRHDFVAQ+VI++LM VQL+TEVPVFSAVLTPHHFH Sbjct: 65 AKVLAKTGRYAGIVASGLVVDGGIYRHDFVAQSVISALMQVQLDTEVPVFSAVLTPHHFH 124 Query: 123 SHGEHQQFFQEHFVVKGQEVARACADTVVKVARIEAGQGNVAVLNAA 169 SH EH +F +HF+VKG+E A+ACA TV K+A++ AG A LNAA Sbjct: 125 SHSEHHDYFHQHFLVKGREAAQACATTVAKLAQVRAGAQ--ASLNAA 169 >ref|YP_004754714.1| 6,7-dimethyl-8-ribityllumazine synthase [Collimonas fungivorans Ter331] gb|AEK63891.1| 6,7-dimethyl-8-ribityllumazine synthase [Collimonas fungivorans Ter331] Length = 215 Score = 224 bits (571), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 106/157 (67%), Positives = 128/157 (81%) Query: 13 NRIAFIQASWHKEIVDQCRISFTAEIVKLGFAESSIDFFELPGAFEIPLHAKLLAKTGKY 72 RIAFIQA WH++IVDQCRI+F AEI K G++E IDFFE+ GAFEIPLHAKLLA + +Y Sbjct: 59 ERIAFIQAGWHRDIVDQCRIAFIAEIAKAGYSEQDIDFFEVAGAFEIPLHAKLLANSSRY 118 Query: 73 AGIVAAGLVVDGGIYRHDFVAQAVITSLMNVQLETEVPVFSAVLTPHHFHSHGEHQQFFQ 132 A +VA GLVVDGGIYRH+FVAQAVI+ LM VQLET VPV SAVLTPHHFH+ EH ++F Sbjct: 119 AAVVATGLVVDGGIYRHEFVAQAVISGLMQVQLETGVPVLSAVLTPHHFHAGEEHHKYFF 178 Query: 133 EHFVVKGQEVARACADTVVKVARIEAGQGNVAVLNAA 169 EHF+VKG E ARACADT+ K+ +++GQ V L++A Sbjct: 179 EHFLVKGAEAARACADTIQKLRLLKSGQPAVRALHSA 215 >ref|YP_004355724.1| riboflavin synthase [Pseudomonas brassicacearum subsp. brassicacearum NFM421] gb|AEA70720.1| Riboflavin synthase [Pseudomonas brassicacearum subsp. brassicacearum NFM421] Length = 169 Score = 222 bits (566), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 107/149 (71%), Positives = 122/149 (81%) Query: 5 SSNTSSARNRIAFIQASWHKEIVDQCRISFTAEIVKLGFAESSIDFFELPGAFEIPLHAK 64 S + S R+AFIQA WHKEIVDQ R F AE++ G+ ES IDFFE+ GAFEIPLHAK Sbjct: 8 SKSKSHPGERVAFIQACWHKEIVDQSRKGFVAEMITQGYQESDIDFFEVGGAFEIPLHAK 67 Query: 65 LLAKTGKYAGIVAAGLVVDGGIYRHDFVAQAVITSLMNVQLETEVPVFSAVLTPHHFHSH 124 LLAK+G+YAGIVAAGLVVDGGIYRH+FVAQ+VI+ LM VQLETEVPVFS VLTPHHFH+ Sbjct: 68 LLAKSGRYAGIVAAGLVVDGGIYRHEFVAQSVISGLMQVQLETEVPVFSVVLTPHHFHAG 127 Query: 125 GEHQQFFQEHFVVKGQEVARACADTVVKV 153 EHQ+FF EHFV KGQE A+ CADT+ K Sbjct: 128 EEHQKFFFEHFVHKGQEAAKTCADTLHKT 156