BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0196.p (255 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08872817.1| short chain dehydrogenase [Verminephrobacter ... 316 2e-84 ref|ZP_02465559.1| short chain dehydrogenase [Burkholderia thail... 302 4e-80 ref|ZP_02357821.1| short chain dehydrogenase [Burkholderia oklah... 301 5e-80 ref|ZP_02364981.1| short chain dehydrogenase [Burkholderia oklah... 301 9e-80 ref|YP_996811.1| short chain dehydrogenase [Verminephrobacter ei... 300 1e-79 >ref|ZP_08872817.1| short chain dehydrogenase [Verminephrobacter sp. At4] Length = 252 Score = 316 bits (810), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 158/255 (61%), Positives = 197/255 (77%), Gaps = 3/255 (1%) Query: 1 MNASPKQGYDFSGRVAIVTGAASGIGLAVSELFASCGAQVVLLDRQTEVMQVATRLTGGI 60 M++S ++ +D SG+VA++TG ASGIGLAV+ELFA GA++VLLD V+ +A +L G Sbjct: 1 MSSSFERAFDLSGQVALITGGASGIGLAVAELFADRGARLVLLDLSESVLALAQQLPG-- 58 Query: 61 DRHLGCVLDVTDPEGIAQVMKQVMARYGRIDVLVNNAGVALLEAADSLSEAAWDATMAIN 120 +HL V +V D + + + + R+ RID+LVNNAGV LL+ AD++ EA WD TMAIN Sbjct: 59 -QHLAVVCNVADTTQLKSAVGRALERFARIDILVNNAGVGLLDRADAVQEADWDKTMAIN 117 Query: 121 LKAPFLLAQAVAPAMRRQAYGRIVNLASQASVIGLERHAAYCASKAGLVGMSKVLALEWA 180 LKAPF LAQAVAP M Q GRIVNLASQASVI LERHAAYCASKA LV +++VLALEWA Sbjct: 118 LKAPFFLAQAVAPVMAGQGGGRIVNLASQASVIALERHAAYCASKAALVSLTQVLALEWA 177 Query: 181 RDGVTVNAVSPTVVETELGKQAWAGAAGQEMRRRIPAGRFAQPQEVACLIAYLASEQAAM 240 G+TVNAVSPTVVETELGK+AWAG G+ M+++IP GRFAQP E+A I YL S A M Sbjct: 178 PLGITVNAVSPTVVETELGKRAWAGEVGEAMKKKIPTGRFAQPAEIAAAILYLVSGHAGM 237 Query: 241 ITGENILIDGGYSVQ 255 ITGEN++IDGGY++Q Sbjct: 238 ITGENLVIDGGYTIQ 252 >ref|ZP_02465559.1| short chain dehydrogenase [Burkholderia thailandensis MSMB43] Length = 256 Score = 302 bits (773), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 154/250 (61%), Positives = 192/250 (76%), Gaps = 1/250 (0%) Query: 7 QGYDFSGRVAIVTGAASGIGLAVSELFASCGAQVVLLDRQTEVMQVATRLTGGIDRHLGC 66 + +D +GRVA+VTG A+GIG A ++L A GA V L+DR E M++A L GG RH G Sbjct: 7 RAFDLTGRVALVTGGAAGIGHACAQLLAQRGASVALVDRHPETMRIAATLEGGAARHSGM 66 Query: 67 VLDVTDPEGIAQVMKQVMARYGRIDVLVNNAGVALLEAADSLSEAAWDATMAINLKAPFL 126 LD+ D ++ V +R+G +D+LVN+AGVALL+ A + EAAWDATMAIN+KA F Sbjct: 67 SLDLRDCSAAQAAVELVASRFGGVDMLVNSAGVALLDKALDVGEAAWDATMAINVKASFF 126 Query: 127 LAQAVAPAMRRQAYG-RIVNLASQASVIGLERHAAYCASKAGLVGMSKVLALEWARDGVT 185 +AQAVA M G RIVNLASQASV+GL+RHAAYCASKA +VGM+KVLALEWA G+T Sbjct: 127 VAQAVARQMIAGGRGGRIVNLASQASVVGLDRHAAYCASKAAIVGMTKVLALEWAPHGIT 186 Query: 186 VNAVSPTVVETELGKQAWAGAAGQEMRRRIPAGRFAQPQEVACLIAYLASEQAAMITGEN 245 VNAVSPT+VETELGKQAWAG AG+ +R IPAGRFAQP E+A L+ YL S+ AAM+TGEN Sbjct: 187 VNAVSPTIVETELGKQAWAGEAGERAKREIPAGRFAQPGEIAALVLYLLSDAAAMMTGEN 246 Query: 246 ILIDGGYSVQ 255 ++IDGGY+V+ Sbjct: 247 VVIDGGYTVR 256 >ref|ZP_02357821.1| short chain dehydrogenase [Burkholderia oklahomensis EO147] Length = 256 Score = 301 bits (772), Expect = 5e-80, Method: Compositional matrix adjust. Identities = 153/250 (61%), Positives = 191/250 (76%), Gaps = 1/250 (0%) Query: 7 QGYDFSGRVAIVTGAASGIGLAVSELFASCGAQVVLLDRQTEVMQVATRLTGGIDRHLGC 66 + +D +GRVA+VTG A+GIG A ++L A GA V L+DR E MQ+A L GG RH G Sbjct: 7 RAFDLTGRVALVTGGAAGIGHACAQLLAQRGASVALVDRHPETMQIAATLKGGAARHAGM 66 Query: 67 VLDVTDPEGIAQVMKQVMARYGRIDVLVNNAGVALLEAADSLSEAAWDATMAINLKAPFL 126 LD+ D ++ V +R+G +D+LVN+AGVALL+ A + EAAWDATMAIN+KA F Sbjct: 67 SLDLRDCSAAQAAVELVASRFGGVDMLVNSAGVALLDKALDVGEAAWDATMAINVKASFF 126 Query: 127 LAQAVAPAM-RRQAYGRIVNLASQASVIGLERHAAYCASKAGLVGMSKVLALEWARDGVT 185 +AQAVA M GRIVNLASQASV+GL+RH AYCASKA +VGM+KVLALEWA G+T Sbjct: 127 VAQAVARQMIAGGGGGRIVNLASQASVVGLDRHVAYCASKAAIVGMTKVLALEWAPHGIT 186 Query: 186 VNAVSPTVVETELGKQAWAGAAGQEMRRRIPAGRFAQPQEVACLIAYLASEQAAMITGEN 245 VNAVSPT+VETELGK+AWAG AG+ +R IPAGRFAQP E+A L+ YL S+ AAM+TGEN Sbjct: 187 VNAVSPTIVETELGKKAWAGEAGERAKREIPAGRFAQPDEIAALVLYLLSDAAAMMTGEN 246 Query: 246 ILIDGGYSVQ 255 ++IDGGY+V+ Sbjct: 247 VVIDGGYTVR 256 >ref|ZP_02364981.1| short chain dehydrogenase [Burkholderia oklahomensis C6786] Length = 256 Score = 301 bits (770), Expect = 9e-80, Method: Compositional matrix adjust. Identities = 152/250 (60%), Positives = 192/250 (76%), Gaps = 1/250 (0%) Query: 7 QGYDFSGRVAIVTGAASGIGLAVSELFASCGAQVVLLDRQTEVMQVATRLTGGIDRHLGC 66 + +D +GRVA+VTG A+GIG A ++L A GA V L+DR E M++A L GG RH+G Sbjct: 7 RAFDLTGRVALVTGGAAGIGHACAQLLAQRGASVALVDRHPETMRIAATLAGGAARHVGM 66 Query: 67 VLDVTDPEGIAQVMKQVMARYGRIDVLVNNAGVALLEAADSLSEAAWDATMAINLKAPFL 126 LD+ D ++ V +R+G +D+LVN+AGVALL+ A + EAAWDATMAIN+KA F Sbjct: 67 SLDLRDCSAAQAAVELVASRFGGVDMLVNSAGVALLDKALDVGEAAWDATMAINVKASFF 126 Query: 127 LAQAVAPAM-RRQAYGRIVNLASQASVIGLERHAAYCASKAGLVGMSKVLALEWARDGVT 185 +AQAVA M GRIVNLASQASV+GL+RH AYCASKA +VGM+KVLALEWA G+T Sbjct: 127 VAQAVARQMIAGGGGGRIVNLASQASVVGLDRHVAYCASKAAIVGMTKVLALEWAPHGIT 186 Query: 186 VNAVSPTVVETELGKQAWAGAAGQEMRRRIPAGRFAQPQEVACLIAYLASEQAAMITGEN 245 VNAVSPT+VETELGK+AWAG AG+ +R IPAGRFAQP E+A L+ YL S+ AAM+TGEN Sbjct: 187 VNAVSPTIVETELGKKAWAGEAGERAKREIPAGRFAQPDEIAALVLYLLSDAAAMMTGEN 246 Query: 246 ILIDGGYSVQ 255 ++IDGGY+V+ Sbjct: 247 VVIDGGYTVR 256 >ref|YP_996811.1| short chain dehydrogenase [Verminephrobacter eiseniae EF01-2] gb|ABM57793.1| short-chain dehydrogenase/reductase SDR [Verminephrobacter eiseniae EF01-2] Length = 254 Score = 300 bits (769), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 157/251 (62%), Positives = 189/251 (75%), Gaps = 1/251 (0%) Query: 6 KQGYDFSGRVAIVTGAASGIGLAVSELFASCGAQVVLLDRQTEVMQVATRLTGGID-RHL 64 +Q + G+ A++TGAA+GIGLAV+ELFA GA +VLLDR V QVA RL+ G + + Sbjct: 4 EQAFKLDGQTAVITGAAAGIGLAVAELFARQGATLVLLDRDATVQQVAQRLSSGAGAQSV 63 Query: 65 GCVLDVTDPEGIAQVMKQVMARYGRIDVLVNNAGVALLEAADSLSEAAWDATMAINLKAP 124 G V DVT + I + Q +A GR+D+LVNNAGV LL A +EA WD TMAINLKAP Sbjct: 64 GLVCDVTCSDAIEAAVAQAVAVSGRLDILVNNAGVGLLAPALEATEADWDKTMAINLKAP 123 Query: 125 FLLAQAVAPAMRRQAYGRIVNLASQASVIGLERHAAYCASKAGLVGMSKVLALEWARDGV 184 FLLA+A A M+RQ GRIVNLASQASV+ LERHAAYCASKA LV M +VLA+EWA G+ Sbjct: 124 FLLARAAARVMQRQGRGRIVNLASQASVVALERHAAYCASKAALVAMGQVLAIEWAPYGI 183 Query: 185 TVNAVSPTVVETELGKQAWAGAAGQEMRRRIPAGRFAQPQEVACLIAYLASEQAAMITGE 244 TVNAVSPTVVET LGKQAW+GA G+ M+++IP GRFAQP E+A I YL S A M+TG Sbjct: 184 TVNAVSPTVVETALGKQAWSGAVGEAMKKKIPVGRFAQPDEIAAAILYLVSGHAGMVTGA 243 Query: 245 NILIDGGYSVQ 255 N+LIDGGY+VQ Sbjct: 244 NLLIDGGYTVQ 254