BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0129.p (337 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CCB74285.1| conserved protein of unknown function [Streptomy... 478 e-133 ref|YP_002550247.1| aldo-keto reductase [Agrobacterium vitis S4]... 471 e-131 ref|YP_002826096.1| putative oxidoreductase, aldo/keto reductase... 460 e-127 ref|YP_530345.1| aldo/keto reductase [Rhodopseudomonas palustris... 460 e-127 ref|YP_003103473.1| aldo/keto reductase [Actinosynnema mirum DSM... 459 e-127 >emb|CCB74285.1| conserved protein of unknown function [Streptomyces cattleya NRRL 8057] Length = 333 Score = 478 bits (1230), Expect = e-133, Method: Compositional matrix adjust. Identities = 240/337 (71%), Positives = 278/337 (82%), Gaps = 4/337 (1%) Query: 1 MEHRILGRNGLSSSALGLGCMGMSDLYGPADEQAGIATIHAALDAGVTLLDTGDFYGMGH 60 M R LG G +SA+GLGCMGMS LYG AD IAT+HAALDAG+TLLDTGDFYGMGH Sbjct: 1 MRTRTLGTTGPRTSAIGLGCMGMSALYGEADRGESIATVHAALDAGITLLDTGDFYGMGH 60 Query: 61 NEMLIREALRGAGSKGGNSRDKVQLSVKFGAMRDAGGNWVGMDNRPAAVKNFLAYTLRRL 120 NE+L+REAL ++ G SR+ +SVKFGA+R+AGG W+G D RP AV NFLAY+L+RL Sbjct: 61 NELLVREAL---ATRPG-SRENALISVKFGALREAGGGWLGYDGRPEAVHNFLAYSLQRL 116 Query: 121 GTDHIDIYRPARLDPSVPIEDTVGAIAEEIKAGRVRHIGLSEVGADTLRRAHAVHPITDL 180 GTDH+D+YR ARLDP+VPIE+TVGAIAEE++AGRVRHIGLSEVGA TLRRA AV PI+DL Sbjct: 117 GTDHVDVYRIARLDPAVPIEETVGAIAEEVEAGRVRHIGLSEVGAQTLRRAAAVAPISDL 176 Query: 181 QIEYSLISRGIEKDILPTCRELGIGITAYGVLSRGLISGHWDPSRQLAANDFRSHSPRFS 240 QIEYSL SRGIE +ILPT RELGIGITAYGVLSRGL+SGHW R +AA DFR+HSPRFS Sbjct: 177 QIEYSLFSRGIEPEILPTARELGIGITAYGVLSRGLLSGHWSAQRPVAAGDFRAHSPRFS 236 Query: 241 GDNLAHNLSLIDVLRSVAADKQATPAQVAIAWVLAQGSDIVPLVGARRPERLAESLGALK 300 G+NL NLSL++ LR++A DK AT AQ+AIAWVLA+G DIVPLVGARR +RL E+LGAL+ Sbjct: 237 GENLQRNLSLVEALRTIADDKGATVAQIAIAWVLARGEDIVPLVGARRRDRLTEALGALE 296 Query: 301 LTLTAQDLARIEEAIPADAAAGDRYAAHAMAHLDSEK 337 +TL DLA IE A+P DA AG RYAA M HLDSEK Sbjct: 297 VTLDEGDLAAIERAVPHDAVAGTRYAADQMEHLDSEK 333 >ref|YP_002550247.1| aldo-keto reductase [Agrobacterium vitis S4] gb|ACM37237.1| aldo-keto reductase [Agrobacterium vitis S4] Length = 329 Score = 471 bits (1213), Expect = e-131, Method: Compositional matrix adjust. Identities = 233/337 (69%), Positives = 270/337 (80%), Gaps = 8/337 (2%) Query: 1 MEHRILGRNGLSSSALGLGCMGMSDLYGPADEQAGIATIHAALDAGVTLLDTGDFYGMGH 60 M+ LG++G +S LGLGCMGMS +YGP+D +AT+HAA+DAGVTL+DTGDFYGMGH Sbjct: 1 MQTVKLGKSGPQTSRLGLGCMGMSGMYGPSDRTESLATLHAAIDAGVTLIDTGDFYGMGH 60 Query: 61 NEMLIREALRGAGSKGGNSRDKVQLSVKFGAMRDAGGNWVGMDNRPAAVKNFLAYTLRRL 120 NEMLI E L+G RDKVQ+SVKFGA RD G W+G D RP+AVK LAYTL+RL Sbjct: 61 NEMLIGEGLKG------GLRDKVQISVKFGAQRDPAGGWIGYDARPSAVKTALAYTLQRL 114 Query: 121 GTDHIDIYRPARLDPSVPIEDTVGAIAEEIKAGRVRHIGLSEVGADTLRRAHAVHPITDL 180 GTDHIDIYRPARLDP+VPIE+T+GAIAE ++AG VRHIGLSEVGADT+RRA AVHPI DL Sbjct: 115 GTDHIDIYRPARLDPNVPIEETIGAIAEMVQAGYVRHIGLSEVGADTIRRAAAVHPIVDL 174 Query: 181 QIEYSLISRGIEKDILPTCRELGIGITAYGVLSRGLISGHWDPSRQLAANDFRSHSPRFS 240 QIEYSLISRGIE ILPTCRELGIGITAYGVLSRGLISGHW + A DFR+HSPRF Sbjct: 175 QIEYSLISRGIEDQILPTCRELGIGITAYGVLSRGLISGHW--TAPTAPTDFRAHSPRFQ 232 Query: 241 GDNLAHNLSLIDVLRSVAADKQATPAQVAIAWVLAQGSDIVPLVGARRPERLAESLGALK 300 G+N HNL+L++ L VA+ K T AQ AIAWVLA+G DIVPL+GARR +RL ESLG L Sbjct: 233 GENANHNLALVEALTQVASSKGMTTAQAAIAWVLAKGEDIVPLIGARRRDRLKESLGVLD 292 Query: 301 LTLTAQDLARIEEAIPADAAAGDRYAAHAMAHLDSEK 337 TL+ +D+A +E A+P D+AAG RYA MAHLDSEK Sbjct: 293 FTLSQEDIAALEAAVPKDSAAGSRYAEAQMAHLDSEK 329 >ref|YP_002826096.1| putative oxidoreductase, aldo/keto reductase family [Sinorhizobium fredii NGR234] gb|ACP25343.1| putative oxidoreductase, aldo/keto reductase family [Sinorhizobium fredii NGR234] Length = 332 Score = 460 bits (1184), Expect = e-127, Method: Compositional matrix adjust. Identities = 234/339 (69%), Positives = 270/339 (79%), Gaps = 11/339 (3%) Query: 1 MEHRILGRNGLSSSALGLGCMGMSDLYGPADEQAGIATIHAALDAGVTLLDTGDFYGMGH 60 M+ LG +G +A+GLGCMGMS +YGPAD IATIHAA++AG+TLLDTGDFYGMGH Sbjct: 1 MQMVSLGTSGPEVAAIGLGCMGMSGMYGPADRAESIATIHAAIEAGITLLDTGDFYGMGH 60 Query: 61 NEMLIREALRGAGSKGGNSRDKVQLSVKFGAMRDAGGNWVGMDNRPAAVKNFLAYTLRRL 120 NEMLIREAL G RDK+QLSVKFGA+RDA G W G D RP AV+NFLAY+L+RL Sbjct: 61 NEMLIREAL------AGRPRDKLQLSVKFGALRDAAGGWSGYDGRPKAVRNFLAYSLQRL 114 Query: 121 GTDHIDIYRPARLDPSVPIEDTVGAIAEEIKAGRVRHIGLSEVGADTLRRAHAVHPITDL 180 G DHIDIYRP+RLDP VPIE+T+GAIA+ +KAG VRHIGLSEVGA+T+RRA AVHPI+DL Sbjct: 115 GVDHIDIYRPSRLDPDVPIEETIGAIADMVKAGYVRHIGLSEVGAETIRRAAAVHPISDL 174 Query: 181 QIEYSLISRGIEKDILPTCRELGIGITAYGVLSRGLISGHW--DPSRQLAANDFRSHSPR 238 QIEYSLISRGIE ILPTCRELGIGITAYGVLSRGLISGHW D +R + DFR+ SPR Sbjct: 175 QIEYSLISRGIEDQILPTCRELGIGITAYGVLSRGLISGHWQKDAAR---SGDFRAMSPR 231 Query: 239 FSGDNLAHNLSLIDVLRSVAADKQATPAQVAIAWVLAQGSDIVPLVGARRPERLAESLGA 298 F NL NL+L++ LR VAADK AT AQ+AIAWV AQG DIVPLVGARR +RL+E+LG+ Sbjct: 232 FQEGNLEQNLALVEALRRVAADKGATVAQIAIAWVAAQGGDIVPLVGARRRDRLSEALGS 291 Query: 299 LKLTLTAQDLARIEEAIPADAAAGDRYAAHAMAHLDSEK 337 + L A DLA IE A+P DAAAG RY + H+DSE Sbjct: 292 QAVRLDAGDLAAIERAVPKDAAAGARYPEAQLVHMDSEN 330 >ref|YP_530345.1| aldo/keto reductase [Rhodopseudomonas palustris BisB18] gb|ABD86026.1| aldo/keto reductase [Rhodopseudomonas palustris BisB18] Length = 332 Score = 460 bits (1184), Expect = e-127, Method: Compositional matrix adjust. Identities = 236/337 (70%), Positives = 265/337 (78%), Gaps = 7/337 (2%) Query: 1 MEHRILGRNGLSSSALGLGCMGMSDLYGPADEQAGIATIHAALDAGVTLLDTGDFYGMGH 60 M+ R LGR+G + SALGLGCMGMSD YGPAD IATIHAALDAG+TLLDTGDFYG GH Sbjct: 1 MDKRTLGRSGPTVSALGLGCMGMSDFYGPADRGESIATIHAALDAGITLLDTGDFYGTGH 60 Query: 61 NEMLIREALRGAGSKGGNSRDKVQLSVKFGAMRDAGGNWVGMDNRPAAVKNFLAYTLRRL 120 NE+LIREA+ G RD QLSVKFGA+RD G + G D RP AVKNFLAY+L RL Sbjct: 61 NELLIREAV------AGRDRDGFQLSVKFGALRDPAGGFTGYDGRPLAVKNFLAYSLGRL 114 Query: 121 GTDHIDIYRPARLDPSVPIEDTVGAIAEEIKAGRVRHIGLSEVGADTLRRAHAVHPITDL 180 G +HIDIYRPARLDP+VPIEDT+GAI+E ++AG V+HIGLSEV ADT+RRAH VHPI DL Sbjct: 115 GVEHIDIYRPARLDPNVPIEDTIGAISELVQAGYVKHIGLSEVSADTIRRAHKVHPIADL 174 Query: 181 QIEYSLISRGIEKDILPTCRELGIGITAYGVLSRGLISGHWDPSRQLAANDFRSHSPRFS 240 QIEYSLISRGIE DILPTCRELGIG+TAYGVLSRGLISGHW AA DFRS SPRFS Sbjct: 175 QIEYSLISRGIEADILPTCRELGIGVTAYGVLSRGLISGHWQKD-HAAARDFRSVSPRFS 233 Query: 241 GDNLAHNLSLIDVLRSVAADKQATPAQVAIAWVLAQGSDIVPLVGARRPERLAESLGALK 300 G NL NL+L++ LR VAA+ A+ AQVAIAWV AQG+DIVPLVGARR +RL E+LGAL Sbjct: 234 GANLDANLALVERLREVAAELGASVAQVAIAWVAAQGADIVPLVGARRRDRLTEALGALA 293 Query: 301 LTLTAQDLARIEEAIPADAAAGDRYAAHAMAHLDSEK 337 + LT LA + A P D AAG RY + HLDSEK Sbjct: 294 VKLTPAQLATLAGAFPPDVAAGGRYPQEHLVHLDSEK 330 >ref|YP_003103473.1| aldo/keto reductase [Actinosynnema mirum DSM 43827] gb|ACU39627.1| aldo/keto reductase [Actinosynnema mirum DSM 43827] Length = 332 Score = 459 bits (1181), Expect = e-127, Method: Compositional matrix adjust. Identities = 221/333 (66%), Positives = 267/333 (80%), Gaps = 6/333 (1%) Query: 4 RILGRNGLSSSALGLGCMGMSDLYGPADEQAGIATIHAALDAGVTLLDTGDFYGMGHNEM 63 R LG G ++ LGLGCMGMSD+YGP DE+ G+ATIHAALDAGVTLLDTGDFYGMG NE+ Sbjct: 5 RTLGSTGPATYPLGLGCMGMSDMYGPPDEREGVATIHAALDAGVTLLDTGDFYGMGANEL 64 Query: 64 LIREALRGAGSKGGNSRDKVQLSVKFGAMRDAGGNWVGMDNRPAAVKNFLAYTLRRLGTD 123 LIR+ALRG R+ V LSVKFGA RDA G W G D RP AV N+LAY+L+RLG D Sbjct: 65 LIRDALRG------RRREDVLLSVKFGAFRDADGGWHGNDGRPEAVANYLAYSLQRLGVD 118 Query: 124 HIDIYRPARLDPSVPIEDTVGAIAEEIKAGRVRHIGLSEVGADTLRRAHAVHPITDLQIE 183 H+D+YRPARLDP+VPIE+TVGA+AEE++ GRVRHIGLSEV A+TLR+AHAVHPI DLQIE Sbjct: 119 HVDVYRPARLDPNVPIEETVGAVAEEVRKGRVRHIGLSEVSAETLRKAHAVHPIVDLQIE 178 Query: 184 YSLISRGIEKDILPTCRELGIGITAYGVLSRGLISGHWDPSRQLAANDFRSHSPRFSGDN 243 YSL+SRG E +ILPT +ELG+G+TAYGVL RGL+SGHW+P+R+ DFR+HSPRF GDN Sbjct: 179 YSLLSRGPEAEILPTAKELGVGVTAYGVLGRGLLSGHWNPNRETTQADFRTHSPRFQGDN 238 Query: 244 LAHNLSLIDVLRSVAADKQATPAQVAIAWVLAQGSDIVPLVGARRPERLAESLGALKLTL 303 L+ NL+L++ LR+VA K AT AQ+AIAW L++G ++VPLVGAR RLAE+LGAL + L Sbjct: 239 LSKNLALVESLRAVAETKGATVAQLAIAWTLSRGENVVPLVGARTRPRLAEALGALDVHL 298 Query: 304 TAQDLARIEEAIPADAAAGDRYAAHAMAHLDSE 336 T DL IE A+P+ AG RY +AH+DSE Sbjct: 299 TEADLTEIESAVPSTEVAGTRYPEQMLAHMDSE 331