BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0115.p (313 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_587876.1| hypothetical protein Rmet_5748 [Cupriavidus met... 347 2e-93 ref|YP_299717.1| hypothetical protein Reut_B5528 [Ralstonia eutr... 343 2e-92 ref|YP_002874076.1| hypothetical protein PFLU4549 [Pseudomonas f... 340 2e-91 ref|YP_004352590.1| membrane protein [Pseudomonas brassicacearum... 324 1e-86 ref|YP_004215182.1| hypothetical protein Rahaq_4470 [Rahnella sp... 322 6e-86 >ref|YP_587876.1| hypothetical protein Rmet_5748 [Cupriavidus metallidurans CH34] gb|ABF12607.1| conserved hypothetical protein; putative membrane protein [Cupriavidus metallidurans CH34] Length = 303 Score = 347 bits (889), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 187/292 (64%), Positives = 225/292 (77%), Gaps = 3/292 (1%) Query: 1 MKPADLLRLLALSAIWGASFLFMRLAVPEMGALNTAFFRVLFGAAGLLLLLAILRVKWRF 60 M+PADL RLL LSAIWGASFLF+R+ P +G L TAFFRVL GA L L +L +KW Sbjct: 1 MRPADLFRLLLLSAIWGASFLFIRIGAPVLGPLPTAFFRVLIGAVTLGACLPMLGLKWDM 60 Query: 61 QGKLHATMLLGAINSGLPFVLFSLAAKVLPAGYSAILNGTAPLMGVVIGALFFGDTLTSR 120 QGK A ++LG INSG+PFV++++AA LPAGYSAI N PLMGV+IG+LFF + LT Sbjct: 61 QGKWRAVIILGIINSGIPFVMYAIAALWLPAGYSAIFNAMTPLMGVLIGSLFFAEKLTRA 120 Query: 121 KVGGVILGLAGVAVLTRTGPVDFDTATVLGALACLLATSCYGLASFLTKRWITDRGGLDS 180 K GV++GLAGV VLTRTGPV F +LGALACL+ATSCYGLA FLT+RWIT RGGLDS Sbjct: 121 KAAGVLMGLAGVGVLTRTGPVVFSHEVLLGALACLVATSCYGLAGFLTRRWITARGGLDS 180 Query: 181 KLVACGSQLGAVALLLPFFAVMAVLEDGPAAPLHAGIGTWFALLALGFVCSALAYILFFR 240 +LVA GS +GA LLPF AV E+ A AG G W A+LA+G C+A+AY+L+FR Sbjct: 181 RLVAAGSLVGATLFLLPFSAVALWRENTLPA---AGPGVWAAMLAVGMFCTAIAYVLYFR 237 Query: 241 LIADIGPVKSLTVTFLIPLFGVLWGWLFLQEQLSWAYAAGGALIGMALLLVL 292 LIAD+GPV+SLTVTFLIP FG++WGW+FL E LSWA+AAG ALIG+A+ LVL Sbjct: 238 LIADLGPVRSLTVTFLIPPFGIVWGWMFLGESLSWAHAAGMALIGVAVWLVL 289 >ref|YP_299717.1| hypothetical protein Reut_B5528 [Ralstonia eutropha JMP134] gb|AAZ64873.1| Protein of unknown function DUF6, transmembrane [Ralstonia eutropha JMP134] Length = 311 Score = 343 bits (881), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 183/300 (61%), Positives = 225/300 (75%), Gaps = 11/300 (3%) Query: 1 MKPADLLRLLALSAIWGASFLFMRLAVPEMGALNTAFFRVLFGAAGLLLLLAILRVKWRF 60 MKPADL+RLL+L+A WGASFLF+R+ P +G TAF RVL GA L L +L ++W Sbjct: 9 MKPADLVRLLSLAAFWGASFLFLRIGAPILGPFPTAFVRVLIGAVTLAACLPVLGLRWEM 68 Query: 61 QGKLHATMLLGAINSGLPFVLFSLAAKVLPAGYSAILNGTAPLMGVVIGALFFGDTLTSR 120 +GK A ++LG +NSG+PFV++++AA LPAGYSA+ N PLMGV+IGALFF + LT Sbjct: 69 RGKWPAVLMLGVVNSGIPFVMYAVAALWLPAGYSAVFNAMTPLMGVLIGALFFAERLTRA 128 Query: 121 KVGGVILGLAGVAVLTRTGPVDFDTATVLGALACLLATSCYGLASFLTKRWITDRGGLDS 180 K GV+LG+AGVAVLTRTGPV F +LGALACL+AT+CYGLA FLT+RWIT+RGGLDS Sbjct: 129 KAAGVLLGIAGVAVLTRTGPVAFSVHVMLGALACLVATACYGLAGFLTRRWITERGGLDS 188 Query: 181 KLVACGSQLGAVALLLPF----FAVMAVLEDGPAAPLHAGIGTWFALLALGFVCSALAYI 236 +L+A GS +GA LLPF A L D AG G W A+L LG VC+ALAYI Sbjct: 189 RLIAAGSLIGATLFLLPFSVGALAQHNTLPD-------AGAGVWAAMLGLGVVCTALAYI 241 Query: 237 LFFRLIADIGPVKSLTVTFLIPLFGVLWGWLFLQEQLSWAYAAGGALIGMALLLVLMPAR 296 L++RLIAD+GPV+SLTVTFLIP FG++WG LFL E LSWA+ AGGALIG A+ LVL P+R Sbjct: 242 LYYRLIADLGPVRSLTVTFLIPPFGMVWGALFLHESLSWAHLAGGALIGAAVWLVLRPSR 301 >ref|YP_002874076.1| hypothetical protein PFLU4549 [Pseudomonas fluorescens SBW25] emb|CAY51266.1| putative membrane protein [Pseudomonas fluorescens SBW25] Length = 299 Score = 340 bits (871), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 186/299 (62%), Positives = 217/299 (72%), Gaps = 3/299 (1%) Query: 1 MKPADLLRLLALSAIWGASFLFMRLAVPEMGALNTAFFRVLFGAAGLLLLLAILRVKWRF 60 M+P D L LL L+AIWGASFLFMR+ PE+G L TAFFRV AAGLL++LA +RVKW F Sbjct: 1 MRPVDTLYLLGLAAIWGASFLFMRIIAPEIGTLPTAFFRVSIAAAGLLVILAAMRVKWDF 60 Query: 61 QGKLHATMLLGAINSGLPFVLFSLAAKVLPAGYSAILNGTAPLMGVVIGALFFGDTLTSR 120 QGK +LLG INSG+P ++S+AA+VLPAGYSAI N T PLMGV+IG LFF + LT Sbjct: 61 QGKFKTVLLLGVINSGIPATMYSVAAQVLPAGYSAIFNATTPLMGVLIGGLFFSERLTPS 120 Query: 121 KVGGVILGLAGVAVLTRTGPVDFDTATVLGALACLLATSCYGLASFLTKRWITDRGGLDS 180 KV GV LGL GV VLTR GPV FD ++GALACLLAT+CYG A FL +RW+ RGGLDS Sbjct: 121 KVAGVCLGLLGVGVLTRAGPVAFDLELLMGALACLLATTCYGFAGFLARRWLDQRGGLDS 180 Query: 181 KLVACGSQLGAVALLLPFFAVMAVLEDGPAAPLHAGIGTWFALLALGFVCSALAYILFFR 240 +L A GS LGA LLPFFA AV + PA+ G W +LL LG VC+A AYIL+FR Sbjct: 181 RLSALGSMLGATLFLLPFFAYSAVSQP-PAS--WGGWQVWLSLLGLGLVCTAFAYILYFR 237 Query: 241 LIADIGPVKSLTVTFLIPLFGVLWGWLFLQEQLSWAYAAGGALIGMALLLVLMPARAGA 299 L+ IGPVKS+TVTF+IP FGVLWG L L E LS A+ GG LI AL LVL P + A Sbjct: 238 LLTAIGPVKSMTVTFMIPPFGVLWGALLLDEPLSMAHLYGGVLIAGALWLVLRPKKLSA 296 >ref|YP_004352590.1| membrane protein [Pseudomonas brassicacearum subsp. brassicacearum NFM421] gb|AEA67586.1| Conserved hypothetical protein; membrane protein [Pseudomonas brassicacearum subsp. brassicacearum NFM421] Length = 302 Score = 324 bits (831), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 177/294 (60%), Positives = 211/294 (71%), Gaps = 3/294 (1%) Query: 1 MKPADLLRLLALSAIWGASFLFMRLAVPEMGALNTAFFRVLFGAAGLLLLLAILRVKWRF 60 M D+LRLL+L+AIWGASFLFMR+ P +G + TAFFRV GLL++LA++RV W F Sbjct: 1 MNLVDILRLLSLAAIWGASFLFMRIIAPVIGTIPTAFFRVSIAFVGLLVILALMRVDWNF 60 Query: 61 QGKLHATMLLGAINSGLPFVLFSLAAKVLPAGYSAILNGTAPLMGVVIGALFFGDTLTSR 120 +GKL M LG INSG+P +SLAA+VLPAGYSAI N T PLMGV+IG LFF + LT Sbjct: 61 RGKLKVVMGLGLINSGIPATFYSLAAQVLPAGYSAIFNATTPLMGVLIGGLFFSEKLTPA 120 Query: 121 KVGGVILGLAGVAVLTRTGPVDFDTATVLGALACLLATSCYGLASFLTKRWITDRGGLDS 180 KV GV LGL GV +LTR GPV FD ++GALACLLAT+CYG A FLT+RW+ +GGLDS Sbjct: 121 KVSGVCLGLFGVGILTRAGPVAFDLQLLMGALACLLATTCYGFAGFLTRRWLDQQGGLDS 180 Query: 181 KLVACGSQLGAVALLLPFFAVMAVLEDGPAAPLHAGIGTWFALLALGFVCSALAYILFFR 240 +L A GS GA LLP F +V+ PA+ G W +LL LG VC+ALAYIL+FR Sbjct: 181 RLSALGSMFGATVFLLPLFG-YSVISQPPAS--WGGWSVWLSLLGLGLVCTALAYILYFR 237 Query: 241 LIADIGPVKSLTVTFLIPLFGVLWGWLFLQEQLSWAYAAGGALIGMALLLVLMP 294 L++ IGPVKS+TVTFLIP FGVLWG L L E L A+ GG LI AL LVL P Sbjct: 238 LLSSIGPVKSMTVTFLIPPFGVLWGALLLDEPLGMAHLYGGVLIAAALWLVLKP 291 >ref|YP_004215182.1| hypothetical protein Rahaq_4470 [Rahnella sp. Y9602] gb|ADW76055.1| protein of unknown function DUF6 transmembrane [Rahnella sp. Y9602] Length = 307 Score = 322 bits (825), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 176/312 (56%), Positives = 221/312 (70%), Gaps = 9/312 (2%) Query: 1 MKPADLLRLLALSAIWGASFLFMRLAVPEMGALNTAFFRVLFGAAGLLLLLAILRVKWRF 60 MK D RLL L+AIWG SFLFMR+A P GA+NTAF RVLFG GL+++LA+LR + Sbjct: 1 MKSGDYCRLLLLAAIWGGSFLFMRVAAPAFGAINTAFLRVLFGFVGLVVILAVLRTPRDY 60 Query: 61 QGKLHATMLLGAINSGLPFVLFSLAAKVLPAGYSAILNGTAPLMGVVIGALFFGDTLTSR 120 Q K ++LLG INSGLPF+++ LAA+ LPAGYSAILN T PLMGVV+G LFF +T+T + Sbjct: 61 QHKFGRSLLLGVINSGLPFLMYCLAARWLPAGYSAILNATTPLMGVVLGGLFFHETVTLK 120 Query: 121 KVGGVILGLAGVAVLTRTGPVDFDTATVLGALACLLATSCYGLASFLTKRWITDRGGLDS 180 K GV+ GL G+ +LT TG + + G +ACL+AT CY +A FLT+RWI++RGGLD Sbjct: 121 KALGVLTGLFGITLLTTTGSAESVPQLLAGVIACLVATGCYAVAGFLTRRWISERGGLDP 180 Query: 181 KLVACGSQLGAVALLLPFFAVMAVLEDGP---AAPLHAGIGTWFALLALGFVCSALAYIL 237 KLVA GSQLGA LLPFF + E P A PL W +LA+GF+C+A AYIL Sbjct: 181 KLVATGSQLGATLFLLPFFLYTSATE-APVTWAQPL-----VWLCVLAVGFICTAWAYIL 234 Query: 238 FFRLIADIGPVKSLTVTFLIPLFGVLWGWLFLQEQLSWAYAAGGALIGMALLLVLMPARA 297 +FRLIADIGP++S+TVTFLIP FG+LWG+L L E LS + AGG LI +A+ LVL P ++ Sbjct: 235 YFRLIADIGPLRSMTVTFLIPPFGILWGYLLLNETLSEGFFAGGILICVAVWLVLSPGKS 294 Query: 298 GAGGNKQKIDGK 309 AG K G Sbjct: 295 VAGKTKTPAPGT 306