BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0053.p (236 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776678.1| bifunctional folylpolyglutamate synthase/dih... 409 e-112 ref|YP_001353851.1| bifunctional folylpolyglutamate synthase / d... 367 e-100 ref|YP_001099529.1| bifunctional folylpolyglutamate synthase/dih... 363 9e-99 ref|YP_004752531.1| folylpolyglutamate synthase [Collimonas fung... 359 1e-97 ref|ZP_08273835.1| Dihydrofolate synthase [Oxalobacteraceae bact... 344 5e-93 >ref|YP_003776678.1| bifunctional folylpolyglutamate synthase/dihydrofolate synthase [Herbaspirillum seropedicae SmR1] gb|ADJ64770.1| bifunctional folylpolyglutamate synthase/dihydrofolate synthase protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 409 bits (1050), Expect = e-112, Method: Compositional matrix adjust. Identities = 209/236 (88%), Positives = 221/236 (93%) Query: 1 MPPKSLIKHAESIGADLWLMGRDFNYQGDKQQWAYGGRAQRRNSLAYPSLRGANQLLNAS 60 +PPKSLI HAE IGADLWLMGRDFNYQGDKQQWAYGGR RRNSLAYPSLRGANQLLNAS Sbjct: 202 LPPKSLIAHAEKIGADLWLMGRDFNYQGDKQQWAYGGRGMRRNSLAYPSLRGANQLLNAS 261 Query: 61 AALAALEALRDLLPIGAQEVRTGLATVELPGRFQVLPGQPLVILDVAHNPHAAATLAQNL 120 AALAALEALR++LP+GAQEVRTGLATVELPGRFQVLPGQPLVILDVAHNPHAAATLAQNL Sbjct: 262 AALAALEALREVLPVGAQEVRTGLATVELPGRFQVLPGQPLVILDVAHNPHAAATLAQNL 321 Query: 121 DNMGFHPYTYAVFGSMLDKDIEGVISHLKGKIDHWCVTDLPLPRAATAQQLKDKLLEAGV 180 DNMGFHPYTYAVFGSMLDKDIEGVI HLK KIDHWCVTDLPLPRAA+AQQLKD LL AGV Sbjct: 322 DNMGFHPYTYAVFGSMLDKDIEGVIGHLKDKIDHWCVTDLPLPRAASAQQLKDALLGAGV 381 Query: 181 EPEFKPDAACTIETFESPAAAFANAQSRATENDRIVVFGSFLTVAGVMEANKPELH 236 +PEF+ DAA +IETF +PAAA+ANA SRA ENDRIVVFGSFLTVAGVM+A +P LH Sbjct: 382 QPEFRRDAARSIETFTTPAAAYANALSRAGENDRIVVFGSFLTVAGVMQARRPGLH 437 >ref|YP_001353851.1| bifunctional folylpolyglutamate synthase / dihydrofolate synthase [Janthinobacterium sp. Marseille] gb|ABR89349.1| bifunctional folylpolyglutamate synthase / dihydrofolate synthase [Janthinobacterium sp. Marseille] Length = 432 Score = 367 bits (942), Expect = e-100, Method: Compositional matrix adjust. Identities = 178/236 (75%), Positives = 197/236 (83%) Query: 1 MPPKSLIKHAESIGADLWLMGRDFNYQGDKQQWAYGGRAQRRNSLAYPSLRGANQLLNAS 60 +PP SL++HAE IGADLWL RDFNY GDKQQW + GR +RRNSL YPSLRGANQLLNAS Sbjct: 197 VPPASLVEHAEKIGADLWLFNRDFNYSGDKQQWNFSGRGRRRNSLGYPSLRGANQLLNAS 256 Query: 61 AALAALEALRDLLPIGAQEVRTGLATVELPGRFQVLPGQPLVILDVAHNPHAAATLAQNL 120 + LAALEALR LP+GAQEVRTGLA V+LPGRFQVLPGQP VILDVAHNPHAAATLAQNL Sbjct: 257 SVLAALEALRLRLPVGAQEVRTGLALVDLPGRFQVLPGQPTVILDVAHNPHAAATLAQNL 316 Query: 121 DNMGFHPYTYAVFGSMLDKDIEGVISHLKGKIDHWCVTDLPLPRAATAQQLKDKLLEAGV 180 DNM F PYTYAVFG+M DKDI+GVISHLKG++DHWCVTDLPLPRAA A +++DKL+ A Sbjct: 317 DNMNFAPYTYAVFGAMQDKDIDGVISHLKGRVDHWCVTDLPLPRAAGAAEIRDKLIHAYG 376 Query: 181 EPEFKPDAACTIETFESPAAAFANAQSRATENDRIVVFGSFLTVAGVMEANKPELH 236 E D+ +I+TF SPA AFANA SRA ENDRI VFGSFLTVAGVME E H Sbjct: 377 EVPPAGDSERSIQTFTSPAEAFANALSRAGENDRIAVFGSFLTVAGVMEFRNSEHH 432 >ref|YP_001099529.1| bifunctional folylpolyglutamate synthase/dihydrofolate synthase [Herminiimonas arsenicoxydans] emb|CAL61402.1| FolC bifunctional protein [Includes: Folylpolyglutamate synthase (Folylpoly-gamma-glutamate synthetase) (FPGS) (Tetrahydrofolate synthase) (Tetrahydrofolylpolyglutamate synthase); Dihydrofolate synthase ] [Herminiimonas arsenicoxydans] Length = 433 Score = 363 bits (933), Expect = 9e-99, Method: Compositional matrix adjust. Identities = 175/236 (74%), Positives = 194/236 (82%) Query: 1 MPPKSLIKHAESIGADLWLMGRDFNYQGDKQQWAYGGRAQRRNSLAYPSLRGANQLLNAS 60 +PP SLI+HAE IGADLWL RDFNY GDKQQW + GR RRNSLAYPSLRGANQLLNAS Sbjct: 198 VPPASLIEHAEKIGADLWLFNRDFNYSGDKQQWNFSGRGHRRNSLAYPSLRGANQLLNAS 257 Query: 61 AALAALEALRDLLPIGAQEVRTGLATVELPGRFQVLPGQPLVILDVAHNPHAAATLAQNL 120 + LAALE LR LP+GAQEVRTGLA V+LPGRFQVLPGQP ++LDVAHNPHAAA LAQNL Sbjct: 258 SVLAALEVLRLRLPVGAQEVRTGLALVDLPGRFQVLPGQPTIVLDVAHNPHAAAALAQNL 317 Query: 121 DNMGFHPYTYAVFGSMLDKDIEGVISHLKGKIDHWCVTDLPLPRAATAQQLKDKLLEAGV 180 NMGFHPYTYAVFG+M DKDI+GVI+HLKG++DHWCVTDLPLPRAATA ++DKLL A Sbjct: 318 GNMGFHPYTYAVFGAMQDKDIDGVIAHLKGQVDHWCVTDLPLPRAATAADIRDKLLHAYA 377 Query: 181 EPEFKPDAACTIETFESPAAAFANAQSRATENDRIVVFGSFLTVAGVMEANKPELH 236 + + +I TF +PA AFANA SRA ENDRI VFGSFLTVAGVME E H Sbjct: 378 DLPPAVETERSIRTFATPADAFANALSRAGENDRITVFGSFLTVAGVMEFRHSEHH 433 >ref|YP_004752531.1| folylpolyglutamate synthase [Collimonas fungivorans Ter331] gb|AEK61708.1| Folylpolyglutamate synthase [Collimonas fungivorans Ter331] Length = 440 Score = 359 bits (922), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 181/236 (76%), Positives = 205/236 (86%) Query: 1 MPPKSLIKHAESIGADLWLMGRDFNYQGDKQQWAYGGRAQRRNSLAYPSLRGANQLLNAS 60 +PP+SLI HA +IGADLWL+GRDFNY GDKQQW YGGR QRRN+L YPSLRGANQLLNAS Sbjct: 205 VPPQSLIDHANAIGADLWLLGRDFNYTGDKQQWNYGGRGQRRNALGYPSLRGANQLLNAS 264 Query: 61 AALAALEALRDLLPIGAQEVRTGLATVELPGRFQVLPGQPLVILDVAHNPHAAATLAQNL 120 AALAALEALRD LP+ AQEVR+G ++LPGRFQVLPGQPLVILDVAHNPHAAATLAQNL Sbjct: 265 AALAALEALRDRLPVSAQEVRSGFVMMDLPGRFQVLPGQPLVILDVAHNPHAAATLAQNL 324 Query: 121 DNMGFHPYTYAVFGSMLDKDIEGVISHLKGKIDHWCVTDLPLPRAATAQQLKDKLLEAGV 180 DNMGFHPYTYAVFG+MLDKDIEG+++ LK +IDHWC+T LPLPRAA+ +QL+++LLEAGV Sbjct: 325 DNMGFHPYTYAVFGAMLDKDIEGIVAQLKTRIDHWCLTSLPLPRAASGEQLQERLLEAGV 384 Query: 181 EPEFKPDAACTIETFESPAAAFANAQSRATENDRIVVFGSFLTVAGVMEANKPELH 236 E PDA +I +F+SP A+ANA SRA ENDRIVVFGSFLTVAGVM A K H Sbjct: 385 HAEPGPDAQSSILSFDSPELAYANALSRAGENDRIVVFGSFLTVAGVMAARKSGFH 440 >ref|ZP_08273835.1| Dihydrofolate synthase [Oxalobacteraceae bacterium IMCC9480] gb|EGF32699.1| Dihydrofolate synthase [Oxalobacteraceae bacterium IMCC9480] Length = 433 Score = 344 bits (883), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 172/228 (75%), Positives = 197/228 (86%) Query: 1 MPPKSLIKHAESIGADLWLMGRDFNYQGDKQQWAYGGRAQRRNSLAYPSLRGANQLLNAS 60 +PP+SL+ HA ++GADLWL+GRDFNY GD+ QW YGGR QRRNSL YPSLRGANQLLNA+ Sbjct: 198 VPPQSLLDHAAAVGADLWLLGRDFNYMGDQLQWNYGGREQRRNSLGYPSLRGANQLLNAA 257 Query: 61 AALAALEALRDLLPIGAQEVRTGLATVELPGRFQVLPGQPLVILDVAHNPHAAATLAQNL 120 AALAALE LR LP+GAQEVRTGL V+LPGRFQVLPG+P VILDVAHNPHAAATL+QN Sbjct: 258 AALAALEVLRLRLPVGAQEVRTGLVLVDLPGRFQVLPGRPTVILDVAHNPHAAATLSQNF 317 Query: 121 DNMGFHPYTYAVFGSMLDKDIEGVISHLKGKIDHWCVTDLPLPRAATAQQLKDKLLEAGV 180 NMGFHPYTYAVFG+M DKDI+GVI+ LKG++DHWC+TDLPLPR ATA+Q+++KLL AGV Sbjct: 318 GNMGFHPYTYAVFGAMHDKDIDGVIAQLKGQVDHWCLTDLPLPRGATAEQIREKLLAAGV 377 Query: 181 EPEFKPDAACTIETFESPAAAFANAQSRATENDRIVVFGSFLTVAGVM 228 P K DA CTI+ F +PA AFANA RA ENDRI VFGSF+TVAGVM Sbjct: 378 TPTDKADAECTIQCFATPAEAFANATGRAGENDRIAVFGSFVTVAGVM 425