BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0010.p (465 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_998697.1| ABC transporter nitrate-binding protein [Vermin... 876 0.0 ref|YP_547155.1| ABC transporter nitrate-binding protein [Polaro... 691 0.0 ref|YP_001155777.1| ABC transporter nitrate-binding protein [Pol... 684 0.0 ref|ZP_08405888.1| twin-arginine translocation pathway signal pr... 679 0.0 ref|ZP_07044278.1| putative nitrate transporter component, nrtA ... 678 0.0 >ref|YP_998697.1| ABC transporter nitrate-binding protein [Verminephrobacter eiseniae EF01-2] gb|ABM59679.1| ABC transporter nitrate-binding protein [Verminephrobacter eiseniae EF01-2] Length = 471 Score = 876 bits (2264), Expect = 0.0, Method: Compositional matrix adjust. Identities = 423/471 (89%), Positives = 443/471 (94%), Gaps = 6/471 (1%) Query: 1 MPKEIDSTIFHTYDAERAGNNCSCGRHASQA--AHDD----EQQRQLQVRAQDPEQLSND 54 MP+EIDS +FHT DA AGN CSCG+HASQ AH D QQ ++R+QDPE LSND Sbjct: 1 MPREIDSPLFHTDDAGYAGNICSCGKHASQMQMAHGDGHPAAQQHLRRIRSQDPEPLSND 60 Query: 55 VVEESVMRALFPQAEQRRDFLRAVGINTARAAIASMFPLGALQAMAQEKNGAIEKKDLKI 114 VVEESV+RALFPQA QRR FLRAVG NTARAAIASMFPL ALQAMAQEKNGAIEKKDLKI Sbjct: 61 VVEESVLRALFPQAAQRRGFLRAVGANTARAAIASMFPLDALQAMAQEKNGAIEKKDLKI 120 Query: 115 GFIAITCAAPLIMADPLGFYKKQGLNVSLNKTAGWALIRDKMINKEHDASHFLSPMPLAM 174 GFIA+TCAAPLIMADPLGFY+KQGLNVSLNKTAGWALIRDKMINKE+DASHFLSPMPLAM Sbjct: 121 GFIALTCAAPLIMADPLGFYRKQGLNVSLNKTAGWALIRDKMINKEYDASHFLSPMPLAM 180 Query: 175 SMGVGSNPVQMRVATIQNINGQAITLHVKHKDKRDPKQWKGFRFAVPFEYSMHNFLLRYY 234 S+G GS+PVQMR+ATIQNINGQAITLHVKHKDKR+P QW GFRFAVPFEYSMHNFLLRYY Sbjct: 181 SIGAGSHPVQMRIATIQNINGQAITLHVKHKDKRNPGQWNGFRFAVPFEYSMHNFLLRYY 240 Query: 235 LAENGIDPDRDVQIRVTPPPEMVANLRAGNIDGFLGPDPFNQRAVYDEVGFIHILSKEIW 294 LAENG+DPDRDVQIRVTPPPEMVANLRAGNIDGFLGPDPFNQRAVYDEVGFIHILSKEIW Sbjct: 241 LAENGLDPDRDVQIRVTPPPEMVANLRAGNIDGFLGPDPFNQRAVYDEVGFIHILSKEIW 300 Query: 295 DGHPCCAFGMSEDFVKQNPNTFAALFRAVLTAAAMARDPANRSLVAKVISPAAYLNQPET 354 DGHPCCAFGMSEDFVKQNPNTFAALFRAVLTAAAMARDPANRSLVAKVISPAAYLNQPET Sbjct: 301 DGHPCCAFGMSEDFVKQNPNTFAALFRAVLTAAAMARDPANRSLVAKVISPAAYLNQPET 360 Query: 355 VVEQVLTGRFADGLGNVKNVPGRADFDPVPWDSMAVWILSQMKRWGYVKGEVDYKGISER 414 VVEQVLTGRFADGLGNVK VPGRADFDPVPWDSMAVWILSQ+KRWGYVKGE+DYKGI+ER Sbjct: 361 VVEQVLTGRFADGLGNVKTVPGRADFDPVPWDSMAVWILSQLKRWGYVKGEIDYKGIAER 420 Query: 415 VLLLTDAKKYMKELGQPVPDGAYRKYKIMDKEFDPAKADAYVNSFAIKRTT 465 VLLLTDAKKYMKELGQPVPDGAYRK+ IM KEFDPAKADAYVNSFAIKRT+ Sbjct: 421 VLLLTDAKKYMKELGQPVPDGAYRKHMIMGKEFDPAKADAYVNSFAIKRTS 471 >ref|YP_547155.1| ABC transporter nitrate-binding protein [Polaromonas sp. JS666] gb|ABE42257.1| ABC transporter nitrate-binding protein [Polaromonas sp. JS666] Length = 451 Score = 691 bits (1784), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/458 (71%), Positives = 386/458 (84%), Gaps = 10/458 (2%) Query: 7 STIFHTYDAERAGN-NCSCGRHASQAAHDDEQQRQLQVRAQDPEQLSNDVVEESVMRALF 65 S F YDA+R C+CG+ + HD A ++LS+ +E S+++ALF Sbjct: 2 SNRFDAYDADRPLMLRCACGQDHAPGEHD--------AAAASAQELSHSFMEASLVKALF 53 Query: 66 PQAEQRRDFLRAVGINTARAAIASMFPLGALQAMAQEKNGAIEKKDLKIGFIAITCAAPL 125 P RR FLRAVG NTARAAIAS+FP+GALQAMAQ++ G +EKKDLKIGFIAITCA+PL Sbjct: 54 PVDSVRRSFLRAVGANTARAAIASVFPMGALQAMAQDR-GPLEKKDLKIGFIAITCASPL 112 Query: 126 IMADPLGFYKKQGLNVSLNKTAGWALIRDKMINKEHDASHFLSPMPLAMSMGVGSNPVQM 185 IMADPLGFYKK+GL+V LNKTAGWALIRDKMINKEHDASHFLSPMPLAM+MG+GSN V M Sbjct: 113 IMADPLGFYKKEGLHVQLNKTAGWALIRDKMINKEHDASHFLSPMPLAMTMGLGSNQVNM 172 Query: 186 RVATIQNINGQAITLHVKHKDKRDPKQWKGFRFAVPFEYSMHNFLLRYYLAENGIDPDRD 245 VA+IQN NGQAITLHVKHK+ RDPK WKGF+FA+PFEYSMHNFLLRYYLAENG++PD D Sbjct: 173 NVASIQNTNGQAITLHVKHKNNRDPKNWKGFKFAIPFEYSMHNFLLRYYLAENGLNPDTD 232 Query: 246 VQIRVTPPPEMVANLRAGNIDGFLGPDPFNQRAVYDEVGFIHILSKEIWDGHPCCAFGMS 305 VQ+RV PPPEMVANLRAGNIDGFLGPDPFNQRAVYDEVGFIHILSK+IWDGHPCCAFG+S Sbjct: 233 VQLRVVPPPEMVANLRAGNIDGFLGPDPFNQRAVYDEVGFIHILSKDIWDGHPCCAFGVS 292 Query: 306 EDFVKQNPNTFAALFRAVLTAAAMARDPANRSLVAKVISPAAYLNQPETVVEQVLTGRFA 365 ++F+++NPNTFAAL+RAVL A+ MA P +R L+AKVI+P YLNQPE V++QVLTG+FA Sbjct: 293 DEFIQKNPNTFAALYRAVLGASFMASQPKDRDLIAKVIAPTQYLNQPEAVLQQVLTGKFA 352 Query: 366 DGLGNVKNVPGRADFDPVPWDSMAVWILSQMKRWGYVKGEVDYKGISERVLLLTDAKKYM 425 DGLGN+KNVP RA+FDPVPW SMAVW+L+QMKRWGYVKG+V+Y+ I+E+V LLT+AKK M Sbjct: 353 DGLGNIKNVPDRANFDPVPWQSMAVWMLTQMKRWGYVKGDVNYRQIAEKVFLLTEAKKQM 412 Query: 426 KELGQPVPDGAYRKYKIMDKEFDPAKADAYVNSFAIKR 463 G P+GAY+K+K+M KEFDPAK + YV SFAI + Sbjct: 413 SLAGFKPPEGAYKKFKVMGKEFDPAKPEDYVKSFAINK 450 >ref|YP_001155777.1| ABC transporter nitrate-binding protein [Polynucleobacter necessarius subsp. asymbioticus QLW-P1DMWA-1] gb|ABP34213.1| ABC transporter nitrate-binding protein [Polynucleobacter necessarius subsp. asymbioticus QLW-P1DMWA-1] Length = 457 Score = 684 bits (1764), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/459 (70%), Positives = 381/459 (83%), Gaps = 11/459 (2%) Query: 10 FHTYDAERA--GNNCSCGRHASQAAHDDEQQRQLQVRAQDPEQLSNDVVEESVMRALFPQ 67 + +D +R N CSCG+HAS+ H + + D EQ S D VE S+++ALFPQ Sbjct: 4 YRPFDPDRPLFSNRCSCGQHASELDHANSLSAMV-----DAEQQSADFVEASLVKALFPQ 58 Query: 68 AEQRRDFLRAVGINTARAAIASMFPLGALQAMAQEKNGAIEKKDLKIGFIAITCAAPLIM 127 ++RR FL+AVG+ A +A++ P+G+LQAMAQEK +EK DLKIGFIAITCA PLIM Sbjct: 59 EDRRRAFLKAVGVGGAMSALSGFLPVGSLQAMAQEK-APLEKPDLKIGFIAITCATPLIM 117 Query: 128 ADPLGFYKKQGLNVSLNKTAGWALIRDKMINKEHDASHFLSPMPLAMSMGVGSNPVQMRV 187 ADPLGFYKKQGL V+LNKTAGWALIRDKM+NKEHDASHFLSPMP++MSMG+GS+P QMRV Sbjct: 118 ADPLGFYKKQGLKVTLNKTAGWALIRDKMLNKEHDASHFLSPMPISMSMGLGSDPSQMRV 177 Query: 188 ATIQNINGQAITLHVKHKDKRDPKQWKGFRFAVPFEYSMHNFLLRYYLAENGIDPDRDVQ 247 ATIQN+NGQAITL KHKD RDPK WKG +FAVPFEYSMHNFLLRYY+A+ G+DPD+D+Q Sbjct: 178 ATIQNVNGQAITLANKHKDNRDPKNWKGMKFAVPFEYSMHNFLLRYYVAQAGLDPDKDIQ 237 Query: 248 IRVTPPPEMVANLRAGNIDGFLGPDPFNQRAVYDEVGFIHILSKEIWDGHPCCAFGMSED 307 IRVTPPPEMVANLRAGNIDGFLGPDPFNQRAVYDEVGFIHIL+K+IWDGHPCCAFG SE+ Sbjct: 238 IRVTPPPEMVANLRAGNIDGFLGPDPFNQRAVYDEVGFIHILTKQIWDGHPCCAFGTSEE 297 Query: 308 FVKQNPNTFAALFRAVLTAAAMARDPANRSLVAKVISPAAYLNQPETVVEQVLTGRFADG 367 F+++NPNTFAAL+RAVL A+ MARDPANR L+AKVIS YLNQPETVV QVLTG+FADG Sbjct: 298 FIRKNPNTFAALYRAVLNASTMARDPANRPLIAKVISTPNYLNQPETVVMQVLTGKFADG 357 Query: 368 LGNVKNVPGRADFDPVPWDSMAVWILSQMKRWGYVKGEVDYKGISERVLLLTDAKKYMKE 427 LGNV+NVP R DF+P+PW SMA W+L+QM+RWGYVKG+V+YK ISE+V LLTDAKKYM E Sbjct: 358 LGNVQNVPDRIDFNPIPWYSMATWMLTQMQRWGYVKGDVNYKDISEKVFLLTDAKKYMGE 417 Query: 428 LGQPVPDGA---YRKYKIMDKEFDPAKADAYVNSFAIKR 463 G VP A Y+K KIM KEFDP++ AY+ SF + Sbjct: 418 TGIAVPPLAKVGYKKDKIMGKEFDPSQPAAYLKSFQTNK 456 >ref|ZP_08405888.1| twin-arginine translocation pathway signal protein [Hylemonella gracilis ATCC 19624] gb|EGI77009.1| twin-arginine translocation pathway signal protein [Hylemonella gracilis ATCC 19624] Length = 477 Score = 679 bits (1753), Expect = 0.0, Method: Compositional matrix adjust. Identities = 316/462 (68%), Positives = 383/462 (82%), Gaps = 3/462 (0%) Query: 4 EIDSTIFHTYDAERAGN-NCSCGRHASQAAHDDEQQRQLQVR-AQDPEQLSNDVVEESVM 61 + D+ + YDA+R CSCG+ + A H A + E LS D VE S++ Sbjct: 15 DTDAQTYAPYDADRPLMLRCSCGQDHAPADHAASMAATRSASLATEAEALSRDFVEASLI 74 Query: 62 RALFPQAEQRRDFLRAVGINTARAAIASMFPLGALQAMAQEKNGAIEKKDLKIGFIAITC 121 +A+FP RR FL+AVG NTARAAIAS+ P+ ALQAMAQ+K GA+EKKD+KIGFI ITC Sbjct: 75 KAIFPVDSVRRRFLKAVGANTARAAIASLLPVSALQAMAQDK-GALEKKDIKIGFIPITC 133 Query: 122 AAPLIMADPLGFYKKQGLNVSLNKTAGWALIRDKMINKEHDASHFLSPMPLAMSMGVGSN 181 A PLIMA P+GFY +QGLNV + KTAGWALIRDKM+NKE+DA+HFLSPMPLAMS+G+GSN Sbjct: 134 ATPLIMAHPMGFYSQQGLNVEVVKTAGWALIRDKMLNKEYDATHFLSPMPLAMSLGLGSN 193 Query: 182 PVQMRVATIQNINGQAITLHVKHKDKRDPKQWKGFRFAVPFEYSMHNFLLRYYLAENGID 241 P M+VA IQN NGQAITL +KHKDKRDPK WKGF+FAVPF+YSMHNFLLRYY+AE+G++ Sbjct: 194 PAPMQVAAIQNTNGQAITLALKHKDKRDPKDWKGFKFAVPFDYSMHNFLLRYYVAEHGLN 253 Query: 242 PDRDVQIRVTPPPEMVANLRAGNIDGFLGPDPFNQRAVYDEVGFIHILSKEIWDGHPCCA 301 PD D+QIRV PPPEMVANLRAGNIDG+LGPDPFNQRAVY+E+GFIH+L+K++WDGHPCCA Sbjct: 254 PDTDIQIRVVPPPEMVANLRAGNIDGYLGPDPFNQRAVYEEIGFIHLLTKDLWDGHPCCA 313 Query: 302 FGMSEDFVKQNPNTFAALFRAVLTAAAMARDPANRSLVAKVISPAAYLNQPETVVEQVLT 361 FG S DF++QNPNTFAAL+RAVLT+AAMAR NR L+AK I+PA YLNQPE V++QVLT Sbjct: 314 FGTSADFIQQNPNTFAALYRAVLTSAAMARKAENRELIAKTIAPAQYLNQPEIVLQQVLT 373 Query: 362 GRFADGLGNVKNVPGRADFDPVPWDSMAVWILSQMKRWGYVKGEVDYKGISERVLLLTDA 421 G+FADGLG V+NVP RADFDP+PW SMAVW+L+QM+RWGY++G+V+YK I+E+V LLTDA Sbjct: 374 GKFADGLGKVQNVPNRADFDPMPWQSMAVWMLTQMQRWGYLQGDVNYKQIAEKVFLLTDA 433 Query: 422 KKYMKELGQPVPDGAYRKYKIMDKEFDPAKADAYVNSFAIKR 463 KK M LGQPVP+G+Y K+KIM +EFDPAKA Y SF I++ Sbjct: 434 KKQMAALGQPVPEGSYPKFKIMGREFDPAKAADYAKSFKIRK 475 >ref|ZP_07044278.1| putative nitrate transporter component, nrtA [Comamonas testosteroni S44] gb|EFI62028.1| putative nitrate transporter component, nrtA [Comamonas testosteroni S44] Length = 465 Score = 678 bits (1750), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/464 (68%), Positives = 384/464 (82%), Gaps = 9/464 (1%) Query: 8 TIFHTYDAER-AGNNCSCGRHASQA------AHDDEQQRQLQVRAQDPEQLSNDVVEESV 60 + F +DAE+ +CSCGRH S++ AHD Q+ + + EQ SN+ +E S+ Sbjct: 2 SYFAPFDAEKPLMMSCSCGRHGSESEHKAASAHDAAQELIRRSQTNSFEQYSNEFIEASL 61 Query: 61 MRALFPQAEQRRDFLRAVGINTARAAIASMFPLGALQAMAQEKNGAIEKKDLKIGFIAIT 120 ++A+FPQ RR FLRAVG +TA AAI+S+ PL +LQAMAQ+K GAIEKK LK+GFI IT Sbjct: 62 VKAIFPQDAVRRHFLRAVGKSTAMAAISSVLPLTSLQAMAQDK-GAIEKKSLKVGFIPIT 120 Query: 121 CAAPLIMADPLGFYKKQGLNVSLNKTAGWALIRDKMINKEHDASHFLSPMPLAMSMGVGS 180 CA PLIMA+PLGFY KQGL+V + KTAGWAL+RDK++NKE+DASHFLSPMPLA+SMG+GS Sbjct: 121 CATPLIMAEPLGFYSKQGLDVQIVKTAGWALVRDKVMNKEYDASHFLSPMPLAISMGLGS 180 Query: 181 NPVQMRVATIQNINGQAITLHVKHKDKRDPKQWKGFRFAVPFEYSMHNFLLRYYLAENGI 240 N M VATIQN+NGQAITL +KHK RDPK WKGF+FA+PFEYSMHNFLLRYYLAE G+ Sbjct: 181 NAQPMNVATIQNVNGQAITLALKHKGNRDPKNWKGFKFAIPFEYSMHNFLLRYYLAEAGL 240 Query: 241 DPDRDVQIRVTPPPEMVANLRAGNIDGFLGPDPFNQRAVYDEVGFIHILSKEIWDGHPCC 300 DPDRDVQ+RV PP EMVANLRAGNIDGFLGPDPFNQRAV +EVGFIHIL+++IW+GHPCC Sbjct: 241 DPDRDVQLRVVPPAEMVANLRAGNIDGFLGPDPFNQRAVVEEVGFIHILTRDIWNGHPCC 300 Query: 301 AFGMSEDFVKQNPNTFAALFRAVLTAAAMARDPANRSLVAKVISPAAYLNQPETVVEQVL 360 AFG S +F+++NPNTFAAL+R+VLT+AAMARD NR L+AKVIS AYLNQPE VV QVL Sbjct: 301 AFGTSTEFIQKNPNTFAALYRSVLTSAAMARDAKNRELIAKVISTQAYLNQPEAVVTQVL 360 Query: 361 TGRFADGLGNVKNVPGRADFDPVPWDSMAVWILSQMKRWGYVKGEVDYKGISERVLLLTD 420 TGR+ADGLGNV N P R DFDP+PW SMAVW+L+QMKRWGY+KG+V+YK ++E+V LLTD Sbjct: 361 TGRYADGLGNVINNPARVDFDPIPWQSMAVWMLTQMKRWGYIKGDVNYKQLAEKVFLLTD 420 Query: 421 AKKYMKELGQPVPD-GAYRKYKIMDKEFDPAKADAYVNSFAIKR 463 AK+ MK+LGQ VPD AY Y+IM K FDP+KADAY NSF IK+ Sbjct: 421 AKQTMKQLGQGVPDTTAYPSYQIMGKTFDPSKADAYANSFTIKK 464