BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0001.p (560 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776709.1| Na+(K+)/H+ antiporter protein [Herbaspirillu... 847 0.0 ref|YP_001894063.1| Na+/H+ antiporter [Burkholderia phytofirmans... 505 e-141 ref|ZP_06843660.1| Na+/H+ antiporter [Burkholderia sp. Ch1-1] >g... 494 e-137 ref|YP_556758.1| CPA1 family Na(+)/H(+) antiporter [Burkholderia... 493 e-137 ref|YP_002385177.1| cation/proton antiporter [Escherichia fergus... 489 e-136 >ref|YP_003776709.1| Na+(K+)/H+ antiporter protein [Herbaspirillum seropedicae SmR1] gb|ADJ64801.1| Na+(K+)/H+ antiporter protein [Herbaspirillum seropedicae SmR1] Length = 560 Score = 847 bits (2189), Expect = 0.0, Method: Compositional matrix adjust. Identities = 445/531 (83%), Positives = 491/531 (92%) Query: 1 MEIVFTVLILLLAVAVSGVAARLVPFKIPLPLFQIGLGAVLALPLFGLRISFDPELFLLL 60 MEIVFTVLILLLAVAVSGV ARL PFK+PLPLFQI LGAVLALP+FGLRISFDPELFLLL Sbjct: 1 MEIVFTVLILLLAVAVSGVVARLAPFKLPLPLFQIALGAVLALPMFGLRISFDPELFLLL 60 Query: 61 FIPPLLFTDGWRMPKREFFRLAQPILALALGLVLFTVVGVGYFIHWMIPAIALPSAFALA 120 FIPPLLFTDGWRMPKREFFRLA+PILALALGLV+FTVVGVGYFIHWMIP I+LPSAFALA Sbjct: 61 FIPPLLFTDGWRMPKREFFRLARPILALALGLVMFTVVGVGYFIHWMIPVISLPSAFALA 120 Query: 121 AVLSPTDAVAVSSIIGKRRLPSTMQHVLEGEALMNDASGLVAFKFAIIAVLSGTFSLLDA 180 AVLSPTDAVAVSSI+GK RLP++MQHVLEGEALMNDASGLVAFKFAI AV++GTFSLLDA Sbjct: 121 AVLSPTDAVAVSSIVGKGRLPASMQHVLEGEALMNDASGLVAFKFAIAAVITGTFSLLDA 180 Query: 181 GISFTLIAIGGIAVGLIVAWGFGLIRRKIIEWSGDEPGIQVVLLLLIPFSAYIFAEHFGL 240 ISF LIA GG+AVGLIVAWGFGLIRRKIIEW+GDEPGIQVVLLLLIPF+AYIFAEHFGL Sbjct: 181 SISFVLIAAGGVAVGLIVAWGFGLIRRKIIEWAGDEPGIQVVLLLLIPFTAYIFAEHFGL 240 Query: 241 SGILSAVAAGMLMPYIDTAGAESAATRMQSQSMLTMLDFVFNGMSFILLGLQLPGIVSLA 300 SGIL AVAAGMLMPY++TAG ESAATR+Q +S++TML+FVFNGMSF+LLGLQLPGIVSLA Sbjct: 241 SGILGAVAAGMLMPYLETAGGESAATRLQGRSIMTMLEFVFNGMSFLLLGLQLPGIVSLA 300 Query: 301 HLDAQVAGNVPVWHLFAYIVAITVALITLRFVWVWFNLRLRRFSNAVRGQRARQRYFDVR 360 H+DA++AGNVPVWHLF YIVAIT+AL+ LRFVWVW NLR+RRF++A+RGQ R R FD+R Sbjct: 301 HMDAKMAGNVPVWHLFVYIVAITLALVVLRFVWVWINLRMRRFASALRGQSGRNRKFDLR 360 Query: 361 LISITALAGVRGAITLAGVMSIPLLMPNGHAFPARDLLIFLATGVIVCSLLGGSFLLPWL 420 LISITAL+GVRGAITLAGVMSIPLL+ FPARDLLIFLATGVIVCSLL GS LLPW+ Sbjct: 361 LISITALSGVRGAITLAGVMSIPLLLKEHTPFPARDLLIFLATGVIVCSLLLGSLLLPWM 420 Query: 421 LTGLKLDADNERRRTEETMARLRVSEAAVRSIEEAQERLGADMDQEDLALLTEVSGSLLA 480 L L+LDA+ +RRR EE MARLR+SEAAVRSIE AQER+G ++D+ DLALLTEVSGSLLA Sbjct: 421 LKDLQLDAEVKRRRAEEMMARLRLSEAAVRSIETAQERIGMNLDENDLALLTEVSGSLLA 480 Query: 481 TYRMRMDAESGTEETHRTANRAKHFERELRLEAIRAERREVHHLRAEQQIN 531 TYRMRM+AES +EET R ANR K FERELRLEAIRAER EVH+LRAEQ+IN Sbjct: 481 TYRMRMNAESDSEETQRAANRFKQFERELRLEAIRAERLEVHYLRAEQRIN 531 >ref|YP_001894063.1| Na+/H+ antiporter [Burkholderia phytofirmans PsJN] gb|ACD14839.1| Na+/H+ antiporter [Burkholderia phytofirmans PsJN] Length = 551 Score = 505 bits (1301), Expect = e-141, Method: Compositional matrix adjust. Identities = 272/533 (51%), Positives = 363/533 (68%), Gaps = 7/533 (1%) Query: 1 MEIVFTVLILLLAVAVSGVAARLVPFKIPLPLFQIGLGAVLALPLFGLRISFDPELFLLL 60 M+IVFTVLILLLAVA SGV R++P +PLPL QI +GA+LA P GL ++F+P++F++L Sbjct: 1 MDIVFTVLILLLAVAASGVLTRILPIALPLPLVQIAIGALLAWPRLGLHVTFNPDIFMML 60 Query: 61 FIPPLLFTDGWRMPKREFFRLAQPILALALGLVLFTVVGVGYFIHWMIPAIALPSAFALA 120 FIPPLLF DGWR+PKREFF + IL LALGLV TV+ VGYF+H ++P+I+LP AFALA Sbjct: 61 FIPPLLFADGWRIPKREFFMARRSILMLALGLVFMTVLVVGYFVHALVPSISLPVAFALA 120 Query: 121 AVLSPTDAVAVSSIIGKRRLPSTMQHVLEGEALMNDASGLVAFKFAIIAVLSGTFSLLDA 180 AVLSPTDAVA+S I+GK ++P + H+LEGEALMNDASGLVA KFAI A L+G FSL DA Sbjct: 121 AVLSPTDAVALSGIVGKGKIPGRLMHILEGEALMNDASGLVALKFAIAAALTGVFSLRDA 180 Query: 181 GISFTLIAIGGIAVGLIVAWGFGLIRRKIIEWSGD-EPGIQVVLLLLIPFSAYIFAEHFG 239 ISF +IA+GG+AVG V+W FG + + + + +P VV+ LLIPF+AY+ AE F Sbjct: 181 SISFVIIAVGGLAVGAAVSWLFGYVSAHFLNLTEEGDPAPGVVMTLLIPFAAYLVAERFE 240 Query: 240 LSGILSAVAAGMLMPYIDTAGAESAATRMQSQSMLTMLDFVFNGMSFILLGLQLPGIVSL 299 LSGIL+AVAAGM+M Y A A++R+++ S TM++FVFNGM FILLGLQ P I+ Sbjct: 241 LSGILAAVAAGMMMNYTTMVTAGPASSRVRASSTWTMIEFVFNGMVFILLGLQFPHILGR 300 Query: 300 AHLDAQVAGNVPVWHLFAYIVAITVALITLRFVWVWFNLRLRRF-SNAVRGQRARQRYFD 358 A LDA N VW L YI A+ AL +RFVWVW LR F S + Q Sbjct: 301 ALLDAHETSNAQVWRLIGYIAAVAGALYAMRFVWVWL---LRYFASRSAAKQGVANAVPG 357 Query: 359 VRLISITALAGVRGAITLAGVMSIPLLMPNGHAFPARDLLIFLATGVIVCSLLGGSFLLP 418 +R ++T +AGVRGA+TLAGV+S+P ++P G A P RDL IF+A+GVI+ SL+ LP Sbjct: 358 LRTAAVTTVAGVRGAVTLAGVLSLPEVLPGGAALPGRDLAIFIASGVILVSLIVAVVGLP 417 Query: 419 WLLTGLKLDADNERRRTEETMARLRVSEAAVRSIEEAQERLGADMDQEDLALLTEVSGSL 478 LL+G + D EE +AR ++AA+R+++E +R +++D+ A +V+ + Sbjct: 418 LLLSGWRRGKDPH--AAEEALARTMAAQAAIRAVDEVHDRECSNLDESSSAYAADVTARV 475 Query: 479 LATYRMRMDAESGTEETHRTANRAKHFERELRLEAIRAERREVHHLRAEQQIN 531 + YR R+ E A RA E ++L A+RAER+ + LR+ Q IN Sbjct: 476 MDVYRRRLATLDEEREPRELARRADALEFRMKLAAMRAERKALLELRSSQAIN 528 >ref|ZP_06843660.1| Na+/H+ antiporter [Burkholderia sp. Ch1-1] gb|EFG68744.1| Na+/H+ antiporter [Burkholderia sp. Ch1-1] Length = 551 Score = 494 bits (1273), Expect = e-137, Method: Compositional matrix adjust. Identities = 275/535 (51%), Positives = 363/535 (67%), Gaps = 11/535 (2%) Query: 1 MEIVFTVLILLLAVAVSGVAARLVPFKIPLPLFQIGLGAVLALPLFGLRISFDPELFLLL 60 M+IVFTVLILLLAVA SGV R++P +PLPL QI +GA+LA P GL ++FDPE+F++L Sbjct: 1 MDIVFTVLILLLAVAASGVVTRILPIALPLPLVQIAIGALLAWPKLGLHVTFDPEIFMVL 60 Query: 61 FIPPLLFTDGWRMPKREFFRLAQPILALALGLVLFTVVGVGYFIHWMIPAIALPSAFALA 120 FIPPLLF DGWR+PKREFF + IL LALGLV TV+ VGYF+H ++P+I+LP AFALA Sbjct: 61 FIPPLLFADGWRIPKREFFMARRSILMLALGLVFVTVLVVGYFVHALVPSISLPVAFALA 120 Query: 121 AVLSPTDAVAVSSIIGKRRLPSTMQHVLEGEALMNDASGLVAFKFAIIAVLSGTFSLLDA 180 AVLSPTDAVA+S I+GK ++P + H+LEGEALMNDASGLVA KFAI A L+G FSL DA Sbjct: 121 AVLSPTDAVALSGIVGKGKIPGRLMHILEGEALMNDASGLVALKFAIAAALTGVFSLRDA 180 Query: 181 GISFTLIAIGGIAVGLIVAWGFGLIRRKIIEWSGD-EPGIQVVLLLLIPFSAYIFAEHFG 239 ISF +IA+GG+A G V+W F + + + + D +P VV+ LLIPF+AY+ AE F Sbjct: 181 SISFVIIAVGGLATGAAVSWLFSFVSARFLNLTEDGDPAPGVVMTLLIPFAAYLIAERFE 240 Query: 240 LSGILSAVAAGMLMPYIDTAGAESAATRMQSQSMLTMLDFVFNGMSFILLGLQLPGIVSL 299 LSGIL+AVAAGM M Y A A ++R+++ S TM++FVFNGM FILLGLQ P I+ Sbjct: 241 LSGILAAVAAGMTMNYATLANAGPVSSRVRANSTWTMIEFVFNGMVFILLGLQFPHILDR 300 Query: 300 AHLDAQVAGNVPVWHLFAYIVAITVALITLRFVWVWFNLRLRRFSNAVRGQRARQRYFDV 359 A LDA N V L YI A+ AL +RFVWVW LR F++ RG R V Sbjct: 301 ALLDAHETSNAQVGLLIGYIAAVAAALYAMRFVWVWL---LRWFAS--RGATKRGMANAV 355 Query: 360 ---RLISITALAGVRGAITLAGVMSIPLLMPNGHAFPARDLLIFLATGVIVCSLLGGSFL 416 R +++T +AGVRGA+TLAGV+S+P ++P+G P RDL IF+A+GVI+ SLL Sbjct: 356 PGLRTVAVTTVAGVRGAVTLAGVLSLPEVLPDGVPLPGRDLAIFIASGVILLSLLVAVVA 415 Query: 417 LPWLLTGLKLDADNERRRTEETMARLRVSEAAVRSIEEAQERLGADMDQEDLALLTEVSG 476 LP LL+G + D EE +AR ++AA+R+++E + AD+D+ A +V+ Sbjct: 416 LPLLLSGWRRVKDPH--AAEELLARTMAAQAAIRAVDEVHDTECADLDESASAYAADVTA 473 Query: 477 SLLATYRMRMDAESGTEETHRTANRAKHFERELRLEAIRAERREVHHLRAEQQIN 531 ++ YR R+ +E A RA E ++L A+RAER+ + LR+ Q IN Sbjct: 474 RVMDLYRRRLATLDEEQEPRELARRADALEFRMKLAAMRAERKTLLALRSSQAIN 528 >ref|YP_556758.1| CPA1 family Na(+)/H(+) antiporter [Burkholderia xenovorans LB400] gb|ABE28706.1| sodium/proton antiporter, CPA1 family [Burkholderia xenovorans LB400] Length = 551 Score = 493 bits (1268), Expect = e-137, Method: Compositional matrix adjust. Identities = 273/539 (50%), Positives = 361/539 (66%), Gaps = 19/539 (3%) Query: 1 MEIVFTVLILLLAVAVSGVAARLVPFKIPLPLFQIGLGAVLALPLFGLRISFDPELFLLL 60 M+IVFTVLILLLAVA SGV R++P +PLPL QI +GA+LA P GL ++FDPE+F++L Sbjct: 1 MDIVFTVLILLLAVAASGVVIRILPIALPLPLVQIAIGALLAWPKLGLHVTFDPEIFMML 60 Query: 61 FIPPLLFTDGWRMPKREFFRLAQPILALALGLVLFTVVGVGYFIHWMIPAIALPSAFALA 120 FIPPLLF DGWR+PKREFF + IL LALGLV TV+ VGYF+H ++P+I+LP AFALA Sbjct: 61 FIPPLLFADGWRIPKREFFMARRSILMLALGLVFVTVLVVGYFVHALVPSISLPVAFALA 120 Query: 121 AVLSPTDAVAVSSIIGKRRLPSTMQHVLEGEALMNDASGLVAFKFAIIAVLSGTFSLLDA 180 AVLSPTDAVA+S I+GK ++P + H+LEGEALMNDASGLVA KFAI A L+G FSL DA Sbjct: 121 AVLSPTDAVALSGIVGKGKIPGRLMHILEGEALMNDASGLVALKFAIAAALTGVFSLRDA 180 Query: 181 GISFTLIAIGGIAVGLIVAWGFGLIRRKIIEWSGD-EPGIQVVLLLLIPFSAYIFAEHFG 239 ISF +I++GGIA G V+W F + + + + D +P VV+ LLIPF+AY+ AE F Sbjct: 181 SISFVIISVGGIATGAAVSWLFSFVSARFLNLTEDGDPAPGVVMTLLIPFAAYLIAERFE 240 Query: 240 LSGILSAVAAGMLMPYIDTAGAESAATRMQSQSMLTMLDFVFNGMSFILLGLQLPGIVSL 299 LSGIL+AVAAGM M Y A A ++R+++ S TM++FVFNGM FILLGLQ P I+ Sbjct: 241 LSGILAAVAAGMTMNYATLANAGPVSSRVRANSTWTMIEFVFNGMVFILLGLQFPHILGR 300 Query: 300 AHLDAQVAGNVPVWHLFAYIVAITVALITLRFVWVWF-------NLRLRRFSNAVRGQRA 352 A LDA N V L YI A+ AL +RFVWVW R +NAV G Sbjct: 301 ALLDAHETSNAQVGLLIGYIAAVAAALYAMRFVWVWLLRWVASRGATKRGMANAVPG--- 357 Query: 353 RQRYFDVRLISITALAGVRGAITLAGVMSIPLLMPNGHAFPARDLLIFLATGVIVCSLLG 412 +R +++T +AGVRGA+TLAGV+S+P ++P+G P RDL IF+A+GVI+ SLL Sbjct: 358 ------LRTVAVTTVAGVRGAVTLAGVLSLPEVLPDGVPLPGRDLAIFIASGVILLSLLV 411 Query: 413 GSFLLPWLLTGLKLDADNERRRTEETMARLRVSEAAVRSIEEAQERLGADMDQEDLALLT 472 LP LL+G + D EE +AR ++AA+R+++E + AD+D+ A Sbjct: 412 AVVALPLLLSGWRRGKDPH--AAEELLARTLAAQAAIRAVDEVHDTECADLDESASAYAA 469 Query: 473 EVSGSLLATYRMRMDAESGTEETHRTANRAKHFERELRLEAIRAERREVHHLRAEQQIN 531 +V+ ++ YR R+ +E A RA E ++L A+RAER+ + LR+ Q IN Sbjct: 470 DVTARVMDLYRRRLATLDEEQEPRELARRADALEFRMKLAAMRAERKTLLALRSSQAIN 528 >ref|YP_002385177.1| cation/proton antiporter [Escherichia fergusonii ATCC 35469] emb|CAQ91587.1| putative cation/proton antiporter [Escherichia fergusonii ATCC 35469] gb|EGC06265.1| Na+/H+ antiporter [Escherichia fergusonii B253] Length = 548 Score = 489 bits (1258), Expect = e-136, Method: Compositional matrix adjust. Identities = 269/535 (50%), Positives = 368/535 (68%), Gaps = 13/535 (2%) Query: 1 MEIVFTVLILLLAVAVSGVAARLVPFKIPLPLFQIGLGAVLALPLFGLRISFDPELFLLL 60 MEI FT+LI+ L V++SGV R++PF++PLPL QI +GA+LA P FGL + FDPELFL+L Sbjct: 1 MEIFFTILIMTLVVSLSGVVTRVLPFQVPLPLMQIAIGALLAWPTFGLHVEFDPELFLVL 60 Query: 61 FIPPLLFTDGWRMPKREFFRLAQPILALALGLVLFTVVGVGYFIHWMIPAIALPSAFALA 120 FIPPLLF DGW+ P REF + I LAL LVL TVVG+G+ I+W++P I L AFALA Sbjct: 61 FIPPLLFADGWKTPTREFLEHGREIFGLALALVLVTVVGIGFLIYWVVPGIPLIPAFALA 120 Query: 121 AVLSPTDAVAVSSIIGKRRLPSTMQHVLEGEALMNDASGLVAFKFAIIAVLSGT--FSLL 178 AVLSPTDAVA+S I+G+ R+P + +L+GEALMNDASGLV+ KFA +AV GT F++ Sbjct: 121 AVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGLVSLKFA-VAVAMGTMVFTVG 179 Query: 179 DAGISFTLIAIGGIAVGLIVAWGFGLIRRKIIEWSGDEPGIQVVLLLLIPFSAYIFAEHF 238 A + F +AIGGI G +V+W +G R + W GDEP Q+VLL L+PF++Y+ AEH Sbjct: 180 GATVEFLKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEPATQIVLLFLLPFASYLIAEHI 239 Query: 239 GLSGILSAVAAGMLMPYIDTAGAESAATRMQSQSMLTMLDFVFNGMSFILLGLQLPGIVS 298 G+SGIL+AVAAGM + A R+++ S ML+FVFNGM F+LLGLQLPGI+ Sbjct: 240 GVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWAMLEFVFNGMVFLLLGLQLPGIME 299 Query: 299 LAHLDAQVAGNVPVWHLFAYIVAITVALITLRFVWVWFNLRL-RRFSNAVRGQRARQRYF 357 + + A+ NV VW LF IV I VAL+ +RF W+W +L +RF ++ + + Sbjct: 300 TSLIAAEADPNVEVWMLFMDIVLIYVALMLVRFGWLWTMKKLSKRF---LKKKPMEFGSW 356 Query: 358 DVRLISITALAGVRGAITLAGVMSIPLLMPNGHAFPARDLLIFLATGVIVCSLLGGSFLL 417 R I I + AGVRGAITLAGV+SIPLL+P+G+ FPAR L+FLA GVI+ SL G +L Sbjct: 357 STREILIASFAGVRGAITLAGVLSIPLLLPDGNVFPARYELVFLAAGVILFSLFVGVVML 416 Query: 418 PWLLTGLKLDADNERRRTEETMARLRVSEAAVRSIEEAQERLGADMDQE-DLALLTEVSG 476 P LL +++ AD+ ++ EE +AR +E A+ +I++ +ERL AD ++ D LLTEVS Sbjct: 417 PILLQHIEV-ADHSQQLKEERIARAATAEVAIVAIQKMEERLAADTEENIDTQLLTEVSS 475 Query: 477 SLLATYRMRMDAESGTEETHRTANRAKHFERELRLEAIRAERREVHHLRAEQQIN 531 ++ R R D + E T+ + ++ ER RL A+R+ER E++HLRA ++I+ Sbjct: 476 RVIGNLRRRADGRNNVE----TSLQEENLERRFRLAALRSERAELYHLRATREIS 526