BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0003.p (247 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EGH70368.1| dTDP-glucose 4,6-dehydratase [Pseudomonas syringa... 280 1e-73 ref|YP_002947501.1| NAD-dependent epimerase/dehydratase [Variovo... 280 2e-73 ref|YP_238176.1| dTDP-glucose 4,6-dehydratase [Pseudomonas syrin... 276 2e-72 ref|YP_002912778.1| dTDP-glucose 4,6-dehydratase [Burkholderia g... 276 3e-72 ref|YP_004156126.1| NAD-dependent epimerase/dehydratase [Variovo... 271 5e-71 >gb|EGH70368.1| dTDP-glucose 4,6-dehydratase [Pseudomonas syringae pv. aceris str. M302273PT] Length = 241 Score = 280 bits (717), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 146/241 (60%), Positives = 176/241 (73%) Query: 1 MSETIFLAGATGAIGTVLIPLLRDAGYTVYGGTRRQARADALRELGAIPVMIDVFDADAL 60 MS +F+AGA+G IG L+ LL AGY+VYG TRR + + GA V++DV+DA+ L Sbjct: 1 MSRKVFVAGASGVIGRALLKLLVAAGYSVYGATRRTEKVKDIEATGATAVVVDVYDAERL 60 Query: 61 KSELIRIAPSVVIHQLTDLPRLPDLQALAQAAVGNARVRSEGTRNLVAAALAAGAARLIA 120 ELIRI P V+HQLTDLPR + +A+A NAR+R+EGTRNLVAAA AAGA RLIA Sbjct: 61 NEELIRIQPWAVVHQLTDLPRGLSPELMAKAVENNARIRTEGTRNLVAAARAAGATRLIA 120 Query: 121 QSIAWAYAPGDAPYPEEHPLDIDADGLRRISVDGVVALETQVLQTPGLNGTVLRYGQLYG 180 QSIAWAY PGD PY E PLD++A G R ISV+GV ALE QVL P LNGTVLRYGQLYG Sbjct: 121 QSIAWAYRPGDTPYLETSPLDVEAQGSRGISVEGVAALEQQVLSEPALNGTVLRYGQLYG 180 Query: 181 PGTWAGEPQGSSPVHVAAAAQAALLALQNNASGVFNITEDNTAASNQKARRTLGWNPAFR 240 P T EP G+SPVHV AA+A LLALQ SG+FNIT+D+ SN+KA+R LGW+P+ Sbjct: 181 PDTGTDEPTGTSPVHVQDAARAVLLALQKEQSGIFNITQDSPTVSNEKAKRILGWSPSME 240 Query: 241 S 241 + Sbjct: 241 A 241 >ref|YP_002947501.1| NAD-dependent epimerase/dehydratase [Variovorax paradoxus S110] gb|ACS22235.1| NAD-dependent epimerase/dehydratase [Variovorax paradoxus S110] Length = 249 Score = 280 bits (716), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 158/240 (65%), Positives = 188/240 (78%) Query: 1 MSETIFLAGATGAIGTVLIPLLRDAGYTVYGGTRRQARADALRELGAIPVMIDVFDADAL 60 MSE IF+AGA GAIG+ L+PLL DAGYTVYG TRR RA AL GA+PV++DVFDA AL Sbjct: 1 MSEKIFVAGAAGAIGSALVPLLVDAGYTVYGSTRRTERAQALEASGAVPVVVDVFDAKAL 60 Query: 61 KSELIRIAPSVVIHQLTDLPRLPDLQALAQAAVGNARVRSEGTRNLVAAALAAGAARLIA 120 ++ L+RIAP++VIHQLTDLPR D + +A AA NARVR+EGTRNLVAAA+AAG+ RL+A Sbjct: 61 RAALMRIAPAIVIHQLTDLPRDLDPRRMADAAHRNARVRTEGTRNLVAAAVAAGSRRLVA 120 Query: 121 QSIAWAYAPGDAPYPEEHPLDIDADGLRRISVDGVVALETQVLQTPGLNGTVLRYGQLYG 180 QSIAW YAPG P+ EE PLD+ A+G R +SV GV ALE VL T GL GTVLRYGQ YG Sbjct: 121 QSIAWVYAPGATPHVEEDPLDLAAEGTRGVSVGGVAALERSVLGTAGLTGTVLRYGQFYG 180 Query: 181 PGTWAGEPQGSSPVHVAAAAQAALLALQNNASGVFNITEDNTAASNQKARRTLGWNPAFR 240 PGT EP+G+ PVHV AAA AA+LAL++ GVFNI EDN AS++KARR LGW+ + R Sbjct: 181 PGTSTAEPRGACPVHVEAAAWAAVLALRHARGGVFNIAEDNPEASSEKARRELGWSASLR 240 >ref|YP_238176.1| dTDP-glucose 4,6-dehydratase [Pseudomonas syringae pv. syringae B728a] gb|AAY40138.1| dTDP-glucose 4,6-dehydratase [Pseudomonas syringae pv. syringae B728a] Length = 241 Score = 276 bits (706), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 145/241 (60%), Positives = 174/241 (72%) Query: 1 MSETIFLAGATGAIGTVLIPLLRDAGYTVYGGTRRQARADALRELGAIPVMIDVFDADAL 60 MS +F+AGA+G IG L+ LL A Y+VYG TRR + + GA V++DV+DA+ L Sbjct: 1 MSRKVFVAGASGVIGRALLKLLVAADYSVYGATRRAEKVKDIEATGATAVVVDVYDAERL 60 Query: 61 KSELIRIAPSVVIHQLTDLPRLPDLQALAQAAVGNARVRSEGTRNLVAAALAAGAARLIA 120 EL+RI P VIHQLTDLPR + +A+A NAR+R+EGTRNLVAAA AAGA RLIA Sbjct: 61 NEELVRIQPWAVIHQLTDLPRGLSPELMAKAVENNARIRTEGTRNLVAAARAAGATRLIA 120 Query: 121 QSIAWAYAPGDAPYPEEHPLDIDADGLRRISVDGVVALETQVLQTPGLNGTVLRYGQLYG 180 QSIAWAY PGD PY E PLD++A G R ISV+GV ALE QVL P LNGTVLRYGQLYG Sbjct: 121 QSIAWAYRPGDTPYLETSPLDVEAQGSRGISVEGVAALEQQVLSEPALNGTVLRYGQLYG 180 Query: 181 PGTWAGEPQGSSPVHVAAAAQAALLALQNNASGVFNITEDNTAASNQKARRTLGWNPAFR 240 P T EP G+SPVHV AA+A LLALQ SG+FNIT+D+ SN+KA+R LGW+P Sbjct: 181 PDTGTDEPTGTSPVHVQDAARAVLLALQKEQSGIFNITQDSPTVSNEKAKRILGWSPTVE 240 Query: 241 S 241 + Sbjct: 241 A 241 >ref|YP_002912778.1| dTDP-glucose 4,6-dehydratase [Burkholderia glumae BGR1] gb|ACR30074.1| DTDP-glucose 4,6-dehydratase [Burkholderia glumae BGR1] Length = 245 Score = 276 bits (705), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 150/238 (63%), Positives = 173/238 (72%), Gaps = 1/238 (0%) Query: 1 MSETIFLAGATGAIGTVLIPLLRDAGYTVYGGTRRQARADALRELGAIPVMIDVFDADAL 60 MSE +F+AGATG IG VL+PLL +AGY VYG TRR RA L LGA PV++DVF+AD L Sbjct: 1 MSEKLFVAGATGVIGRVLVPLLVEAGYRVYGATRRAERAALLTGLGAEPVVVDVFNADRL 60 Query: 61 KSELIRIAPSVVIHQLTDLPRLPDLQALAQAAVGNARVRSEGTRNLVAAALAAGAARLIA 120 SEL RIAP+ VIHQLTDLP D +AQA V NAR+R EGTRNLV A+LAAG R+IA Sbjct: 61 TSELRRIAPAAVIHQLTDLPAGLDPSQMAQAIVNNARIRGEGTRNLVQASLAAGCRRMIA 120 Query: 121 QSIAWAYAP-GDAPYPEEHPLDIDADGLRRISVDGVVALETQVLQTPGLNGTVLRYGQLY 179 QSIAWA+A G+ PY E PLD+ A+G R+ISV GV ALE V TP L G VLRYG+LY Sbjct: 121 QSIAWAFASLGEKPYGEAQPLDLAAEGARKISVGGVAALEQAVFDTPELQGAVLRYGRLY 180 Query: 180 GPGTWAGEPQGSSPVHVAAAAQAALLALQNNASGVFNITEDNTAASNQKARRTLGWNP 237 GP T A P + +HV AA+AALL LQ A+GVFNI +DN SNQKARR LGW P Sbjct: 181 GPQTGADAPPAAPALHVNDAARAALLVLQKKATGVFNIVDDNGEVSNQKARRELGWVP 238 >ref|YP_004156126.1| NAD-dependent epimerase/dehydratase [Variovorax paradoxus EPS] gb|ADU38015.1| NAD-dependent epimerase/dehydratase [Variovorax paradoxus EPS] Length = 247 Score = 271 bits (694), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 149/245 (60%), Positives = 186/245 (75%) Query: 1 MSETIFLAGATGAIGTVLIPLLRDAGYTVYGGTRRQARADALRELGAIPVMIDVFDADAL 60 M E IF+AGA GAIG L+PLL +AGY VYG TRR RA+AL G PV++DVFDA+AL Sbjct: 1 MREKIFVAGAAGAIGAALVPLLTEAGYDVYGSTRRADRAEALEARGVTPVVVDVFDAEAL 60 Query: 61 KSELIRIAPSVVIHQLTDLPRLPDLQALAQAAVGNARVRSEGTRNLVAAALAAGAARLIA 120 ++ L+RI P +V+HQLTDLP D + +A+AA NARVR+EGTRNLVAAALAAG+ RL+A Sbjct: 61 RAALLRIRPEIVVHQLTDLPADLDPKLMAEAAHRNARVRTEGTRNLVAAALAAGSHRLVA 120 Query: 121 QSIAWAYAPGDAPYPEEHPLDIDADGLRRISVDGVVALETQVLQTPGLNGTVLRYGQLYG 180 QSIAWAYAPG P+ E+ PLD+++DG+RR+SV GV ALE VL G+ TVLRYGQ+YG Sbjct: 121 QSIAWAYAPGQRPHVEDDPLDLESDGIRRVSVGGVAALERSVLGAVGIKATVLRYGQIYG 180 Query: 181 PGTWAGEPQGSSPVHVAAAAQAALLALQNNASGVFNITEDNTAASNQKARRTLGWNPAFR 240 PGT P+G+SPVHV AAA AA++AL++ GVFNI EDN ++KARR LGW + R Sbjct: 181 PGTSTAVPKGASPVHVEAAAWAAVMALRHAEGGVFNIAEDNAEVISEKARRELGWYASLR 240 Query: 241 SPYGV 245 P G+ Sbjct: 241 LPKGL 245