BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0003.p (378 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003773480.1| periplasmic binding protein [Herbaspirillum ... 541 e-152 ref|YP_004752060.1| small-conductance mechanosensitive ion chann... 531 e-149 ref|YP_558764.1| small-conductance mechanosensitive ion channel ... 365 9e-99 ref|ZP_02379359.1| MscS Mechanosensitive ion channel [Burkholder... 363 3e-98 ref|YP_001895577.1| mechanosensitive ion channel MscS [Burkholde... 360 2e-97 >ref|YP_003773480.1| periplasmic binding protein [Herbaspirillum seropedicae SmR1] gb|ADJ61572.1| periplasmic binding protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 541 bits (1393), Expect = e-152, Method: Compositional matrix adjust. Identities = 262/378 (69%), Positives = 317/378 (83%), Gaps = 7/378 (1%) Query: 1 MEIPYLKDLEPGPLQSGLIVAVAVVVAILLAMVVHRIGILLLRRLAHNRPFTTNATHIAF 60 +++ +L ++ GPL+SGLIV +VA+++A+ +HR+GI ++RRLA RPFTT AT + F Sbjct: 3 IDLDFLYNMNEGPLRSGLIVLGVALVAVVVAVFLHRVGIAVVRRLARGRPFTTTATEVTF 62 Query: 61 RASQVCVALFAVRVVLTAAPDDTPGLRSLSYISSVALIVALTWCVKKCIESVSITITQLN 120 AS+ CV LF +R+VLT APD+TPGL S+SY++S+ LI+ALTW + +C++SVS+T+ LN Sbjct: 63 SASRTCVILFMLRLVLTGAPDNTPGLTSISYLTSIGLILALTWFLIRCVKSVSLTVINLN 122 Query: 121 PYDVADNLKARRILTQTRVLTRSAYFIVGLLGLAFVLLTLPGARQFGASLLASAGVAGLV 180 PYDVADNLKARRILTQTRVL+RSAYFI+ +LGL+FVLLTLPGARQFGASLLASAGVAG+V Sbjct: 123 PYDVADNLKARRILTQTRVLSRSAYFIIAMLGLSFVLLTLPGARQFGASLLASAGVAGIV 182 Query: 181 AGIAARPVLGNFIAGLQIAFSQPIRIDDVLIVNGEWGRVEEITGTFVVVRIWDERRMIVP 240 AGIAA+PVLGNF AGLQIAFSQPIRIDDVLIV GEWGRVEEITGTFVVVRIWDERRMIVP Sbjct: 183 AGIAAKPVLGNFFAGLQIAFSQPIRIDDVLIVKGEWGRVEEITGTFVVVRIWDERRMIVP 242 Query: 241 LQWFIENPFENWTHTSSTILGTVFLWLDFKLPVAALRAEFERVCKAAPQWDGRVCVLQVT 300 LQWFIENPFENWTHTSSTILGTVFLWLDF +P A+R EFERVCK+ P WD RVCV+ VT Sbjct: 243 LQWFIENPFENWTHTSSTILGTVFLWLDFSVPTQAIRDEFERVCKSLPLWDKRVCVMHVT 302 Query: 301 DVNERAMQVRMLVSAQNSGNAFDLRCAIRESMIAFLAEHHPEALPRLRSAIDPVELTPST 360 D +ER MQVR+LVSA++SG+AFDLRC IRE MI F+A ++P ALPRLR+ ++ Sbjct: 303 DASERTMQVRLLVSAKDSGSAFDLRCQIREHMIGFIARNYPGALPRLRAEVEARN----- 357 Query: 361 LADAGVQDKILDGAPTQR 378 D D I+DG PTQ+ Sbjct: 358 --DPEAIDSIIDGPPTQK 373 >ref|YP_004752060.1| small-conductance mechanosensitive ion channel [Collimonas fungivorans Ter331] gb|AEK61237.1| small-conductance mechanosensitive ion channel [Collimonas fungivorans Ter331] Length = 351 Score = 531 bits (1369), Expect = e-149, Method: Compositional matrix adjust. Identities = 255/318 (80%), Positives = 287/318 (90%) Query: 35 HRIGILLLRRLAHNRPFTTNATHIAFRASQVCVALFAVRVVLTAAPDDTPGLRSLSYISS 94 HR+GI+LLRRL NRP+ TNA+ IA+RASQVC+ LFAVR+VLT APDDTP L LSY++S Sbjct: 33 HRVGIMLLRRLVRNRPYITNASFIAYRASQVCLMLFAVRLVLTGAPDDTPWLVPLSYLTS 92 Query: 95 VALIVALTWCVKKCIESVSITITQLNPYDVADNLKARRILTQTRVLTRSAYFIVGLLGLA 154 VALIVALTW +CI ++S T+ QLNP ADNLKARRILTQTRVLTRSAYFI LLGL Sbjct: 93 VALIVALTWLAMRCIRALSATVVQLNPVSAADNLKARRILTQTRVLTRSAYFIAALLGLG 152 Query: 155 FVLLTLPGARQFGASLLASAGVAGLVAGIAARPVLGNFIAGLQIAFSQPIRIDDVLIVNG 214 FVLLTLPGARQFGASLLASAGVAGLVAGIAARPVLGNFIAG+QIAFSQPIRIDDVLIV G Sbjct: 153 FVLLTLPGARQFGASLLASAGVAGLVAGIAARPVLGNFIAGMQIAFSQPIRIDDVLIVQG 212 Query: 215 EWGRVEEITGTFVVVRIWDERRMIVPLQWFIENPFENWTHTSSTILGTVFLWLDFKLPVA 274 EWGRVEEITGTFVVVRIWDERR+IVPLQWFIENPFENWTHTSSTILGTVFLWLDF +PV Sbjct: 213 EWGRVEEITGTFVVVRIWDERRLIVPLQWFIENPFENWTHTSSTILGTVFLWLDFGVPVP 272 Query: 275 ALRAEFERVCKAAPQWDGRVCVLQVTDVNERAMQVRMLVSAQNSGNAFDLRCAIRESMIA 334 A+RAEFER+CK + WDGRVCVL VTD NERAMQ+R+LVSA++SG AFDLRCAIRE+M+A Sbjct: 273 AVRAEFERICKQSSLWDGRVCVLHVTDANERAMQLRLLVSARDSGTAFDLRCAIREAMLA 332 Query: 335 FLAEHHPEALPRLRSAID 352 F+A ++P++LPRLR+A+D Sbjct: 333 FIAGNYPDSLPRLRAALD 350 >ref|YP_558764.1| small-conductance mechanosensitive ion channel [Burkholderia xenovorans LB400] gb|ABE30712.1| small-conductance mechanosensitive ion channel [Burkholderia xenovorans LB400] Length = 407 Score = 365 bits (936), Expect = 9e-99, Method: Compositional matrix adjust. Identities = 181/352 (51%), Positives = 244/352 (69%), Gaps = 3/352 (0%) Query: 3 IPYLKDLE---PGPLQSGLIVAVAVVVAILLAMVVHRIGILLLRRLAHNRPFTTNATHIA 59 +P L DL PL + L + V+A+LLA+ +HRIG + R A P T+ Sbjct: 1 MPTLDDLSRIAEAPLHTWLGALIVSVIAVLLAVGIHRIGARMAARFARPYPLTSVVLRYI 60 Query: 60 FRASQVCVALFAVRVVLTAAPDDTPGLRSLSYISSVALIVALTWCVKKCIESVSITITQL 119 + S + + A+ V APD + L +++ LI A+TW + ++ I Q Sbjct: 61 DKPSLFVLVILALEAVWWEAPDTLTFITPLRDAAAIGLIGAITWLSVRSAAAIGEAIIQA 120 Query: 120 NPYDVADNLKARRILTQTRVLTRSAYFIVGLLGLAFVLLTLPGARQFGASLLASAGVAGL 179 +P D ADNL+ARRI TQ RVL RS F++ ++G+ L+T P RQ GASLLASAGVAGL Sbjct: 121 HPLDTADNLQARRIHTQARVLARSVMFVIVIVGVGGALMTFPNVRQIGASLLASAGVAGL 180 Query: 180 VAGIAARPVLGNFIAGLQIAFSQPIRIDDVLIVNGEWGRVEEITGTFVVVRIWDERRMIV 239 VAGIAARPVLGN IAGLQIA SQPIR+DDV+++ GEWGR+EEITGT+V VR+WD+RR+IV Sbjct: 181 VAGIAARPVLGNLIAGLQIALSQPIRLDDVVVIQGEWGRIEEITGTYVSVRLWDQRRLIV 240 Query: 240 PLQWFIENPFENWTHTSSTILGTVFLWLDFKLPVAALRAEFERVCKAAPQWDGRVCVLQV 299 PLQWFIENPF NWT +SS I+GTVFL++D+++P+A LR E R+ + AP+WD RV VLQV Sbjct: 241 PLQWFIENPFSNWTRSSSQIIGTVFLYVDYRMPLAPLREELARIVEDAPEWDRRVQVLQV 300 Query: 300 TDVNERAMQVRMLVSAQNSGNAFDLRCAIRESMIAFLAEHHPEALPRLRSAI 351 TD ER+MQ+R LVS+ +SG +DLRC +RE ++ F+ +H+P+ LPR R+ + Sbjct: 301 TDGTERSMQLRALVSSLDSGLNWDLRCRVREGLLDFIQKHYPQYLPRARAEV 352 >ref|ZP_02379359.1| MscS Mechanosensitive ion channel [Burkholderia ubonensis Bu] Length = 390 Score = 363 bits (932), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 181/354 (51%), Positives = 240/354 (67%) Query: 6 LKDLEPGPLQSGLIVAVAVVVAILLAMVVHRIGILLLRRLAHNRPFTTNATHIAFRASQV 65 L+ + PL S L + V +L+ HRIG +++R+A P + + S V Sbjct: 7 LRRIADAPLHSWLGTLIVSAVILLVGAAAHRIGARIVKRVASPYPMLSVELRYIDKPSLV 66 Query: 66 CVALFAVRVVLTAAPDDTPGLRSLSYISSVALIVALTWCVKKCIESVSITITQLNPYDVA 125 +AL A+ V A D P +R + ++V IVALTW + + +V I Q +P D A Sbjct: 67 TLALLALEFVWLQADDGMPLVRGMRTAAAVGTIVALTWLLVRLAAAVGEAIIQAHPLDTA 126 Query: 126 DNLKARRILTQTRVLTRSAYFIVGLLGLAFVLLTLPGARQFGASLLASAGVAGLVAGIAA 185 DNL+ARRI TQ RVL+R+ ++ ++G L+T P RQ GASLLASAGVAGLVAGIAA Sbjct: 127 DNLQARRIHTQARVLSRTVMVLIVIVGTGAALMTFPNVRQVGASLLASAGVAGLVAGIAA 186 Query: 186 RPVLGNFIAGLQIAFSQPIRIDDVLIVNGEWGRVEEITGTFVVVRIWDERRMIVPLQWFI 245 RPVLGN IAGLQIA +QPIR+DDV+++ GEWGR+EEITGT+V VR+WD+RR++VPLQWFI Sbjct: 187 RPVLGNLIAGLQIALTQPIRLDDVVVIQGEWGRIEEITGTYVSVRLWDQRRLVVPLQWFI 246 Query: 246 ENPFENWTHTSSTILGTVFLWLDFKLPVAALRAEFERVCKAAPQWDGRVCVLQVTDVNER 305 ENPF NWT S+ I+GTVFL +D++ P+A LR E R+ + AP+WDGRV VLQVTD ER Sbjct: 247 ENPFANWTRNSAEIIGTVFLSVDYRTPLAPLREELARLVQHAPEWDGRVQVLQVTDATER 306 Query: 306 AMQVRMLVSAQNSGNAFDLRCAIRESMIAFLAEHHPEALPRLRSAIDPVELTPS 359 MQ+R LVSA +S FDLRC +RE +IA++ H+P+ LPR R+ + P P Sbjct: 307 TMQLRALVSAADSSLCFDLRCRVREGLIAYIQSHYPQCLPRSRTELFPHASAPD 360 >ref|YP_001895577.1| mechanosensitive ion channel MscS [Burkholderia phytofirmans PsJN] gb|ACD16353.1| MscS Mechanosensitive ion channel [Burkholderia phytofirmans PsJN] Length = 407 Score = 360 bits (924), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 178/352 (50%), Positives = 244/352 (69%), Gaps = 3/352 (0%) Query: 3 IPYLKDLE---PGPLQSGLIVAVAVVVAILLAMVVHRIGILLLRRLAHNRPFTTNATHIA 59 +P L DL PL + + V+A++LA+ +H+IG ++ RLA P T+ Sbjct: 1 MPTLDDLSRMAEAPLHTWPGALIVSVIAMMLAVGIHQIGARIVTRLARPYPLTSVVLRYI 60 Query: 60 FRASQVCVALFAVRVVLTAAPDDTPGLRSLSYISSVALIVALTWCVKKCIESVSITITQL 119 + S + + A+ V APD + L +++ LI +TW + ++ I Q Sbjct: 61 DKPSLFVLVILALEAVWWEAPDTLTFITPLRDAAAITLIGGITWLSVRSAAAIGEAIIQA 120 Query: 120 NPYDVADNLKARRILTQTRVLTRSAYFIVGLLGLAFVLLTLPGARQFGASLLASAGVAGL 179 +P D ADNL+ARRI TQ RVL RS F++ ++G+ L+T P RQ GASLLASAGVAGL Sbjct: 121 HPLDTADNLQARRIHTQARVLARSVMFVIVIVGVGGALMTFPSVRQIGASLLASAGVAGL 180 Query: 180 VAGIAARPVLGNFIAGLQIAFSQPIRIDDVLIVNGEWGRVEEITGTFVVVRIWDERRMIV 239 VAGIAARPVLGN IAGLQIA SQPIR+DDV+++ GEWGR+EEITGT+V VR+WD+RR+IV Sbjct: 181 VAGIAARPVLGNLIAGLQIALSQPIRLDDVVVIQGEWGRIEEITGTYVSVRLWDQRRLIV 240 Query: 240 PLQWFIENPFENWTHTSSTILGTVFLWLDFKLPVAALRAEFERVCKAAPQWDGRVCVLQV 299 PLQWFIENPF NWT +SS I+GTVFL++D+++P+A LR E R+ + AP+WD RV VLQV Sbjct: 241 PLQWFIENPFSNWTRSSSQIIGTVFLYVDYRMPLAPLREELARIVEDAPEWDRRVQVLQV 300 Query: 300 TDVNERAMQVRMLVSAQNSGNAFDLRCAIRESMIAFLAEHHPEALPRLRSAI 351 TD ER+MQ+R LVS+ +SG +DLRC +RE ++ F+ +H+P+ LPR R+ + Sbjct: 301 TDGTERSMQLRALVSSLDSGLNWDLRCRVREGLLDFIQQHYPQYLPRARAEV 352