BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0003.p (363 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777092.1| AMP-binding enzyme family protein [Herbaspir... 395 e-108 ref|YP_004752546.1| acyl-coenzyme A synthetases/AMP-(fatty) acid... 338 6e-91 gb|ADU90654.1| putative AMP-dependent synthetase and ligase [Col... 318 7e-85 ref|YP_586515.1| AMP-dependent synthetase and ligase [Cupriavidu... 251 1e-64 ref|YP_004351602.1| hypothetical protein PSEBR_a454 [Pseudomonas... 249 6e-64 >ref|YP_003777092.1| AMP-binding enzyme family protein [Herbaspirillum seropedicae SmR1] gb|ADJ65184.1| AMP-binding enzyme family protein [Herbaspirillum seropedicae SmR1] Length = 586 Score = 395 bits (1015), Expect = e-108, Method: Compositional matrix adjust. Identities = 222/361 (61%), Positives = 259/361 (71%), Gaps = 15/361 (4%) Query: 2 RPDVSRLQAIFSSGGPLLPDVAAATQQLLGHRPIEIYGSSETGGIAWRQPQELAGTARDD 61 +PD +L+AIFSSGGPL AA + +LLG RP+EIYGSSETGGIAWRQPQ R + Sbjct: 241 QPDARQLRAIFSSGGPLPAQAAADSARLLGQRPLEIYGSSETGGIAWRQPQT-DPLQRQE 299 Query: 62 SWRPMRRVEVRLNSEHDCLEVRSPHLPDDHWLRMADRVEFTSEQNPAQGFYLRGRADRIV 121 W+ M VEVRL +E + LEVRSPHLPD W MADR Q P Q F LRGR+DRIV Sbjct: 300 HWQAMPGVEVRLGAEGE-LEVRSPHLPDSAWFGMADRAALVPGQ-PGQ-FELRGRSDRIV 356 Query: 122 KLEEKRISLDQIERLLLASPLTAEVRVLVHQELPSSSSSSSRRERIAAFVVPTDAGRAVL 181 KLEEKRISL +IE+LL ASPL +VRV+VHQ R+ IAAFVVP+ A+L Sbjct: 357 KLEEKRISLTRIEQLLNASPLVEDVRVIVHQ-------GRRARQFIAAFVVPSAPAHAML 409 Query: 182 EQQGKLALNTQLRAVLADAIESVALPRLWRYLDALPANTQGKTTVAALTALLEQTPAKAA 241 QGKLALNT L+AVLA AIES+ALPRLWRYL ALP N QGK T++ALTALLE + AA Sbjct: 410 AAQGKLALNTALKAVLAGAIESIALPRLWRYLPALPVNPQGKVTLSALTALLE---SPAA 466 Query: 242 ATVSTSRPRWPEQTVLQRDDRSVVLQLHVPPDLLYFDGHFPEAPILPGVVQVDWALALGR 301 A V S P P+ + L+R V L L +P DLLYF GHFP +PILPGVVQ DW+LALGR Sbjct: 467 APV-VSAPTLPQVSELERSSEHVQLALDIPADLLYFRGHFPGSPILPGVVQTDWSLALGR 525 Query: 302 QYFDLPAHFRALQALKFQRVVTPGATLTLELENDTQKNLLAFRLHSAGGQHASGRILFGE 361 +YFDLP HF LQALKFQRV+TPG + L+L+ + K+ L+FRLHS GGQHASGR L G Sbjct: 526 RYFDLPPHFLGLQALKFQRVITPGMRVLLDLQFEAGKSQLSFRLHSEGGQHASGRFLLGA 585 Query: 362 A 362 A Sbjct: 586 A 586 >ref|YP_004752546.1| acyl-coenzyme A synthetases/AMP-(fatty) acid ligase [Collimonas fungivorans Ter331] gb|AEK61723.1| Acyl-coenzyme A synthetases/AMP-(fatty) acid ligase [Collimonas fungivorans Ter331] Length = 604 Score = 338 bits (868), Expect = 6e-91, Method: Compositional matrix adjust. Identities = 189/353 (53%), Positives = 239/353 (67%), Gaps = 17/353 (4%) Query: 8 LQAIFSSGGPLLPDVAAATQQLLGHRPIEIYGSSETGGIAWRQPQELAGTARDDSWRPMR 67 ++ IFSSGGPL +VAAA + LG PIE+YGSSETGG+AWRQ +AG DDSW+ M Sbjct: 263 IRVIFSSGGPLPSEVAAAAGRALGSIPIEVYGSSETGGVAWRQ--RVAGA--DDSWQAMP 318 Query: 68 RVEVRLNSEHDCLEVRSPHLPDDHWLRMADRVEFTSEQNPAQGFYLRGRADRIVKLEEKR 127 V R+ + + LE+ SPHLPD W +AD+V+ Q F L+GR DRIVK+EEKR Sbjct: 319 AVAWRVAANDEVLEISSPHLPDAGWHALADQVQAVDRQR----FRLKGRVDRIVKVEEKR 374 Query: 128 ISLDQIERLLLASPLTAEVRVLVHQELPSSSSSSSRRERIAAFVVPTDAGRAVLEQQGKL 187 ISLD +ER L A L A+ RV++ ++ +R+RIAAFVV + +G +L QGKL Sbjct: 375 ISLDGLERYLKAITLVADARVVLVEQ-----PELQQRQRIAAFVVLSASGSEMLAAQGKL 429 Query: 188 ALNTQLRAVLADAIESVALPRLWRYLDALPANTQGKTTVAALTALLEQTPAKAAATVSTS 247 ALN LR +A A+E++ALPR WRYLDALP N QGKTT A L ALLE A Sbjct: 430 ALNRLLREGIAHAVEAIALPRSWRYLDALPVNAQGKTTRADLLALLEPA----AGAGPAP 485 Query: 248 RPRWPEQTVLQRDDRSVVLQLHVPPDLLYFDGHFPEAPILPGVVQVDWALALGRQYFDLP 307 PR P + +L+++ + V+L+L VP DLLYF+GHF APILPGVVQ+DWA++ GR+YF L Sbjct: 486 APRKPGERLLEQEPQRVLLELTVPADLLYFNGHFEGAPILPGVVQLDWAISYGRRYFALA 545 Query: 308 AHFRALQALKFQRVVTPGATLTLELENDTQKNLLAFRLHSAGGQHASGRILFG 360 HF + LKFQR +TPG + LEL +D QK+ LAFRL S GQHASGRI FG Sbjct: 546 PHFLGMLGLKFQRAITPGMVVQLELLHDLQKSSLAFRLFSVAGQHASGRITFG 598 >gb|ADU90654.1| putative AMP-dependent synthetase and ligase [Collimonas sp. MPS11E8] Length = 616 Score = 318 bits (816), Expect = 7e-85, Method: Compositional matrix adjust. Identities = 181/372 (48%), Positives = 239/372 (64%), Gaps = 29/372 (7%) Query: 8 LQAIFSSGGPLLPDVAAATQQLLGHRPIEIYGSSETGGIAWRQPQELAGTARDDSWRPMR 67 ++ IFSSGGPL + A A +LLG PIE+YGSSETGG+AWRQ +AG DDSW+ M Sbjct: 249 IRVIFSSGGPLPAEAAVAAGRLLGSIPIEVYGSSETGGVAWRQ--RIAGA--DDSWQVMP 304 Query: 68 RVEVRLNSEHDCLEVRSPHLPDDHWLRMADRVEFTSEQNPAQGFYLRGRADRIVKLEEKR 127 + R+ + + LE+RSPHL D W + D+V+ Q F L+GR DRIVK+EEKR Sbjct: 305 AIAWRVAANDELLEIRSPHLADADWHALPDQVQLVGPQR----FCLKGRVDRIVKVEEKR 360 Query: 128 ISLDQIERLLLASPLTAEVRVLVHQELPS-----------SSSSSSRRERIAAFVVPTDA 176 ISLD +ER L A+ RVL+ ++ S S + +R+RIAAFVV + A Sbjct: 361 ISLDALERELKTMAQVADARVLLVEQPESQLNAQPNAQLDSQLKTQQRQRIAAFVVLSAA 420 Query: 177 GRAVLEQQGKLALNTQLRAVLADAIESVALPRLWRYLDALPANTQGKTTVAALTALLEQT 236 G +L QGK LN+ LR +A A+E+VALPR+WRY++++P N QGKTT AAL ALLE Sbjct: 421 GMEILAAQGKPILNSMLRDGMAHAVEAVALPRIWRYVESMPVNAQGKTTRAALLALLESP 480 Query: 237 PAKAAATVSTSRPRWPEQTVLQRDDRSVVLQLHVPPDLLYFDGHFPEAPILPGVVQVDWA 296 P+ + + P +L+++ V+L+L VP DLLYF+GHF APILPGVVQ+DWA Sbjct: 481 PSGSPDLAGLALK--PAVRLLEQESHRVLLELTVPADLLYFNGHFDGAPILPGVVQLDWA 538 Query: 297 LALGRQYFDLPAHFRALQALKFQRVVTPGATLTLE----LENDT----QKNLLAFRLHSA 348 ++ GRQYF L HF + LKFQRV+ PG + LE L++D+ K+ LAFR+ S Sbjct: 539 ISYGRQYFALAPHFLGVLGLKFQRVIIPGMVVQLELLHALKHDSVQEPPKSSLAFRMFST 598 Query: 349 GGQHASGRILFG 360 GQHASGRI FG Sbjct: 599 AGQHASGRITFG 610 >ref|YP_586515.1| AMP-dependent synthetase and ligase [Cupriavidus metallidurans CH34] gb|ABF11246.1| AMP-dependent synthetase and ligase [Cupriavidus metallidurans CH34] Length = 557 Score = 251 bits (641), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 158/354 (44%), Positives = 202/354 (57%), Gaps = 35/354 (9%) Query: 8 LQAIFSSGGPLLPDVAAATQQLLGHRPIEIYGSSETGGIAWRQPQELAGTARDDSWRPMR 67 ++A+FSSGGPL P+ A+ + G PIE++GSSETGG+AWRQ A DD W P+ Sbjct: 232 VRAVFSSGGPLPPEGASDVLRHTGVSPIEVFGSSETGGVAWRQ-----RAAGDDRWTPLP 286 Query: 68 RVEVRLNSEHDCLEVRSPHLPDDHWLRMADRVEFTSEQNPAQGFYLRGRADRIVKLEEKR 127 V RL + L VRS HL DD W ADRV T + F L GRADRIVK+EEKR Sbjct: 287 GVAWRL--QEGFLAVRSAHLADDDWQVCADRVLPTGDG----AFVLAGRADRIVKIEEKR 340 Query: 128 ISLDQIERLLLASPLTAEVRVLVHQELPSSSSSSSRRERIAAFVVPTDAGRAVLEQQGKL 187 +SL IE+ L ASPL E RVL+ + ++ R+ A +V +AG+++ + +G+ Sbjct: 341 VSLSAIEQRLQASPLVREARVLLIE--------TAIGTRVGAAIVLQEAGQSMHDGKGRH 392 Query: 188 ALNTQLRAVLADAIESVALPRLWRYLDALPANTQGKTTVAALTALLEQTPAKAAATVSTS 247 AL LR LAD ++ +ALPR W + ALP N QGKTT A L LL S Sbjct: 393 ALIAALRRALADGVDRLALPRRWAFPQALPCNAQGKTTEAMLRELLRH---------SIP 443 Query: 248 RPRWPEQTVLQRDDRSVVLQLHVPPDLLYFDGHFPEAPILPGVVQVDWALALGRQYFDLP 307 RW E DD S +L + DL FDGHFP AP++PGV QVDW +AL Q +P Sbjct: 444 VARWVEA-----DDTSATAELDIAEDLAIFDGHFPGAPVVPGVAQVDWVMALAPQRLAVP 498 Query: 308 A--HFRALQALKFQRVVTPGATLTLELENDTQKNLLAFRLHSAGGQHASGRILF 359 F L LKFQ V+ PGA + + L+ LAFRL S G HASG+I+F Sbjct: 499 PRQRFARLDVLKFQAVMRPGAAVRMALDWQPASCTLAFRLTSDAGSHASGKIVF 552 >ref|YP_004351602.1| hypothetical protein PSEBR_a454 [Pseudomonas brassicacearum subsp. brassicacearum NFM421] gb|AEA66598.1| Conserved hypothetical protein [Pseudomonas brassicacearum subsp. brassicacearum NFM421] Length = 561 Score = 249 bits (636), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 157/359 (43%), Positives = 200/359 (55%), Gaps = 33/359 (9%) Query: 3 PDVSRLQAIFSSGGPLLPDVAAATQQLLGHRPIEIYGSSETGGIAWRQPQELAGTARDDS 62 P +S ++ +FSSGG L P+ A + QQ LG P EI+GSSETGGIAWRQ L Sbjct: 231 PALSCVRRVFSSGGALPPEAAQSLQQRLGQWPTEIFGSSETGGIAWRQGSGL-------- 282 Query: 63 WRPMRRVEVRLNSEHDCLEVRSPHLPDDHWLRMADRVEFTSEQNPAQGFYLRGRADRIVK 122 W+P VE+ +S+ L + SP+LP H AD + F L GR DRIVK Sbjct: 283 WQPFADVELSQDSD-GALLIASPYLPAGHVEHTADAARIAEDGR----FELLGRLDRIVK 337 Query: 123 LEEKRISLDQIERLLLASPLTAEVRVLVHQELPSSSSSSSRRERIAAFVVPTDAGRAVLE 182 LEEKRISL +E+ L+A +E R+ V QE R + A +V +DAG L Sbjct: 338 LEEKRISLPMLEQALVAHDWVSEARLGVVQE---------NRASLGALLVLSDAGLRALR 388 Query: 183 QQGKLALNTQLRAVLADAIESVALPRLWRYLDALPANTQGKTTVAALTALLEQTPAKAAA 242 QG+ A+ LR L++ E++ALPR WR+L LP N QGK A + ALL Sbjct: 389 NQGRRAVTEGLRRHLSEHCETLALPRRWRWLRQLPLNAQGKLPQAEVEALL--------- 439 Query: 243 TVSTSRPRWPEQTVLQRDDRSVVLQLHVPPDLLYFDGHFPEAPILPGVVQVDWALALGRQ 302 RP+ PE D LQL VPPDL YF GHFP AP+LPGVVQVDWAL+LGRQ Sbjct: 440 --MAPRPKAPEVLEHVETDGEWSLQLVVPPDLAYFSGHFPTAPVLPGVVQVDWALSLGRQ 497 Query: 303 YFDLPAHFRALQALKFQRVVTPGATLTLELENDTQKNLLAFRLHSAGGQHASGRILFGE 361 DLP F ++ LKFQ++V PG + L L D ++ L F + +SGRI+ GE Sbjct: 498 LLDLPPRFVGMEVLKFQQLVRPGDEIQLHLRFDRERGKLYFAYRNDTAACSSGRIVLGE 556