BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0124.p (354 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001901123.1| dienelactone hydrolase [Ralstonia pickettii ... 200 3e-49 ref|YP_002983187.1| dienelactone hydrolase [Ralstonia pickettii ... 199 7e-49 ref|ZP_07675117.1| carboxymethylenebutenolidase [Ralstonia sp. 5... 193 3e-47 ref|ZP_04763898.1| dienelactone hydrolase [Acidovorax delafieldi... 145 7e-33 ref|YP_300001.1| hypothetical protein Reut_B5814 [Ralstonia eutr... 139 1e-30 >ref|YP_001901123.1| dienelactone hydrolase [Ralstonia pickettii 12J] gb|ACD28691.1| dienelactone hydrolase [Ralstonia pickettii 12J] Length = 349 Score = 200 bits (508), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 128/345 (37%), Positives = 190/345 (55%), Gaps = 12/345 (3%) Query: 11 MNSKKIFRHLRRPMQVLMCILTACSLLSWSGLASAQQ--KISFPSAEYANKFEMAKR--V 66 +++ + R L R + V+ I S SAQ +I F S +K+E+ R Sbjct: 4 LSTVGLLRRLFRVVSVMALISAPASAQQIVSDLSAQPTGRIHFNSVTPKSKWELVHRKFA 63 Query: 67 GKPIEIFGYLMLPPNPPAGKIPVVVIAHGSAGVQSKDTGFWAPYFNKLGFAAFVVDSFTP 126 P ++G L LP N G +P +VI+HGSAG Q KD W FN++G AAFVVDSF P Sbjct: 64 KTPTPVWGTLYLPENL-QGPVPAMVISHGSAGPQQKDVDRWVRLFNQMGMAAFVVDSFGP 122 Query: 127 RGVESTVEDQNLVSNAADTADAFFALKFLAADPRFDADRIGIIGFSRGGIAANETAVKSF 186 RG+ +T+++Q+ ++ AA+ ADA FALK L+ DPR D RIG IGFSRGG + + + +F Sbjct: 123 RGIANTMDNQSQLNPAANDADALFALKLLSTDPRIDPKRIGQIGFSRGGGVSLDMTLDAF 182 Query: 187 RDNVLRENKNLQFAFHIPAYPGCQNSRFRKNGSYDKTGAPMLFLMGSKDDYTPAKFCLDQ 246 R V+ + + +FA I YPGC ++ + + TGAP++ +G KDDYTPA+FC + Sbjct: 183 RKGVI--DDDTRFAALIGFYPGC-SALWWEIPPPPLTGAPLMLALGEKDDYTPAQFCRNA 239 Query: 247 IKGMQEEYPGVIDYRVYDGAHHSFDGNQG-VHYLRLGTSTRNCPMTETDIAGWSRH-LVG 304 + MQ + ++ VY GA+H FD + Y T+ R+CP T D+ + + L G Sbjct: 240 AEIMQRDG-QTVEMHVYPGAYHDFDNTRAYFKYFPGATTARDCPQTLIDVQHDAYYRLPG 298 Query: 305 SSDKLSLADEKKMYKDCQSVGVTVGSFDNRYRDLASKDVEDFLRK 349 +L + Y C + GV+ G+ + + A DV FL K Sbjct: 299 GEKYANLQAMQADYTRCTTRGVSTGANLEQAKR-AEADVNAFLVK 342 >ref|YP_002983187.1| dienelactone hydrolase [Ralstonia pickettii 12D] gb|ACS64515.1| dienelactone hydrolase [Ralstonia pickettii 12D] Length = 349 Score = 199 bits (505), Expect = 7e-49, Method: Compositional matrix adjust. Identities = 129/345 (37%), Positives = 190/345 (55%), Gaps = 12/345 (3%) Query: 11 MNSKKIFRHLRRPMQVLMCILTACSLLSWSGLASAQQ--KISFPSAEYANKFEMAKRV-- 66 +++ + R L R + V+ I S SAQ +I F S +K+E+A R Sbjct: 4 LSTVGLLRRLFRVVSVMALISAPASAQQIVSDLSAQPTGRIHFNSVTPKSKWELAHRKFD 63 Query: 67 GKPIEIFGYLMLPPNPPAGKIPVVVIAHGSAGVQSKDTGFWAPYFNKLGFAAFVVDSFTP 126 P I+G L LP N G +P +VI+HGSAG Q KD W FN++G AAFVVDSF P Sbjct: 64 KTPTPIWGTLYLPENL-QGPVPAMVISHGSAGPQQKDVDRWVRLFNQMGMAAFVVDSFGP 122 Query: 127 RGVESTVEDQNLVSNAADTADAFFALKFLAADPRFDADRIGIIGFSRGGIAANETAVKSF 186 RG+ +T+++Q+ ++ AA+ ADA FALK L+ DPR D RIG IGFSRGG + + + +F Sbjct: 123 RGIANTMDNQSQLNPAANDADALFALKLLSTDPRIDPKRIGQIGFSRGGGVSLDMTLDAF 182 Query: 187 RDNVLRENKNLQFAFHIPAYPGCQNSRFRKNGSYDKTGAPMLFLMGSKDDYTPAKFCLDQ 246 R V+ ++ +FA I YPGC ++ + + TGAP++ +G KDDYTPA+ C + Sbjct: 183 RKGVIGDDT--RFAALIGFYPGC-SALWWEIPPPPLTGAPLMLALGEKDDYTPAQLCRNA 239 Query: 247 IKGMQEEYPGVIDYRVYDGAHHSFDGNQG-VHYLRLGTSTRNCPMTETDIAGWSRH-LVG 304 + MQ + ++ VY GA+H FD + Y T+ R+CP T D+ + + L G Sbjct: 240 AEIMQRDG-QTVEVHVYPGAYHDFDNTRAYFKYFPGATTARDCPQTLIDVQHDAYYRLPG 298 Query: 305 SSDKLSLADEKKMYKDCQSVGVTVGSFDNRYRDLASKDVEDFLRK 349 +L + Y C + GV+ G+ + + A DV FL K Sbjct: 299 GEKYANLQAMQADYTRCTTRGVSTGANLEQAKR-AEADVNAFLVK 342 >ref|ZP_07675117.1| carboxymethylenebutenolidase [Ralstonia sp. 5_7_47FAA] gb|EFP66715.1| carboxymethylenebutenolidase [Ralstonia sp. 5_7_47FAA] Length = 328 Score = 193 bits (491), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 123/325 (37%), Positives = 181/325 (55%), Gaps = 21/325 (6%) Query: 40 SGLASAQQ-----------KISFPSAEYANKFEMAKRV--GKPIEIFGYLMLPPNPPAGK 86 S ASAQQ +I F S +K+E+ R P ++G L LP N G Sbjct: 3 SAPASAQQIVSDLSAQPTGRIHFNSVTPKSKWELVHRKFDKTPTPVWGTLYLPENL-QGP 61 Query: 87 IPVVVIAHGSAGVQSKDTGFWAPYFNKLGFAAFVVDSFTPRGVESTVEDQNLVSNAADTA 146 +P +VI+HGSAG Q KD W FN++G AAFVVDSF PRG+ +T+++Q+ ++ AA+ A Sbjct: 62 VPAMVISHGSAGPQQKDVDRWVRLFNQIGMAAFVVDSFGPRGIANTMDNQSQLNPAANDA 121 Query: 147 DAFFALKFLAADPRFDADRIGIIGFSRGGIAANETAVKSFRDNVLRENKNLQFAFHIPAY 206 DA FALK L+ DPR D RIG IGFSRGG + + + +FR V+ + + +FA I Y Sbjct: 122 DALFALKLLSTDPRIDPKRIGQIGFSRGGGVSLDMTLDAFRKGVI--DDDTRFAALIGFY 179 Query: 207 PGCQNSRFRKNGSYDKTGAPMLFLMGSKDDYTPAKFCLDQIKGMQEEYPGVIDYRVYDGA 266 PGC ++ + + TGAP++ +G KDDYTPA+ C + + MQ + ++ VY GA Sbjct: 180 PGC-SALWWEIPPPPLTGAPLMLALGEKDDYTPAQLCRNAAEIMQRDG-QTVEVHVYPGA 237 Query: 267 HHSFDGNQG-VHYLRLGTSTRNCPMTETDIAGWSRHLVGSSDKLS-LADEKKMYKDCQSV 324 +H FD + Y T+ R+CP T D+ + + + +K + L + Y C + Sbjct: 238 YHDFDNTRAYFKYFPGATTARDCPQTLIDVQHDAYYRLPDGEKYANLQAMQADYTRCTTR 297 Query: 325 GVTVGSFDNRYRDLASKDVEDFLRK 349 GV+ G+ + + A DV FL K Sbjct: 298 GVSTGANLEQAK-RAEADVNAFLVK 321 >ref|ZP_04763898.1| dienelactone hydrolase [Acidovorax delafieldii 2AN] gb|EER59301.1| dienelactone hydrolase [Acidovorax delafieldii 2AN] Length = 351 Score = 145 bits (367), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 109/319 (34%), Positives = 162/319 (50%), Gaps = 27/319 (8%) Query: 48 KISFPSAEYANKFEMAK-RVGKPIEIFGYLMLPPNPPAGKIPVVVIAHGSAGVQSKDTGF 106 +I F S+ +++ + + R+G + ++G L++PP GK+P VV +HGS GV S Sbjct: 43 RIEFTSSTPDHRWALIRGRLGPEVTVYGDLLMPPQASNGKVPAVVFSHGSEGVSSLYFDV 102 Query: 107 WAPYFNKLGFAAFVVDSFTPRGVES-TVEDQNLVSNA-ADTADAFFALKFLAADPRFDAD 164 WA N G+A FVVDSF PRG + T + L N A+ DA +ALK LA P+ D Sbjct: 103 WAKALNNAGYAVFVVDSFKPRGEDRVTGPTKQLTWNTMANLTDAMYALKLLATHPQIDNS 162 Query: 165 RIGIIGFSRGGIAANETAVKSFRDNVLRENKNLQFAFHIPAYPGCQNSRFRKNGSYDKTG 224 RI +G+SRGG A + A +++ +VL N+++A + YPGC N R+R + + K Sbjct: 163 RIFHMGWSRGGQVAMDAAWPTYQQHVLPA--NVKWAGGVAVYPGC-NMRYRVD-HHGKLP 218 Query: 225 APMLFLMGSKDDYTPAKFCLDQIKGMQEEYPGV---IDYRVYDGAHHSFDG--------N 273 AP+ ++G KDD T K C++ + E Y + Y+VY GA H FD N Sbjct: 219 APIFLVLGEKDDMTLLKPCME----LAEAYAAAGNPVSYKVYPGATHVFDRLNQPWKKYN 274 Query: 274 QGVHYLRLGTSTRNCPMTETDIAGWSRHLVGSSDKLSLADEKKMY--KDCQSVGVTVGSF 331 +G L + P D + H S + E Y K Q+ VTV S Sbjct: 275 EG--NFNLCSMDVRMPYGANDRSWGPAHDKYSGKTFTDNAEWNAYVPKCRQTSWVTVES- 331 Query: 332 DNRYRDLASKDVEDFLRKI 350 + + R+ A KDV FL+ + Sbjct: 332 NEKAREQAVKDVLAFLKSV 350 >ref|YP_300001.1| hypothetical protein Reut_B5814 [Ralstonia eutropha JMP134] gb|AAZ65157.1| conserved hypothetical protein [Ralstonia eutropha JMP134] Length = 208 Score = 139 bits (349), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 74/149 (49%), Positives = 100/149 (67%), Gaps = 3/149 (2%) Query: 48 KISFPSAEYANKFEMAKRV--GKPIEIFGYLMLPPNPPAGKIPVVVIAHGSAGVQSKDTG 105 +I F S +K+E+ R KP I+G L LP N G++P +VI+HGSAGVQ KDT Sbjct: 48 RIYFNSVTPKSKWELVHRKYDPKPTTIWGMLYLPENV-QGRVPAMVISHGSAGVQDKDTD 106 Query: 106 FWAPYFNKLGFAAFVVDSFTPRGVESTVEDQNLVSNAADTADAFFALKFLAADPRFDADR 165 W FN++G AAFVVDSF RG+ +T+++Q+ +S AA+ ADA FALK L+ DPR D R Sbjct: 107 RWVRVFNQMGVAAFVVDSFGGRGIANTMDNQSQLSPAANDADALFALKLLSTDPRIDPQR 166 Query: 166 IGIIGFSRGGIAANETAVKSFRDNVLREN 194 IG IGFSRGG A + ++SFR V+ ++ Sbjct: 167 IGQIGFSRGGHVAMDLVLESFRKGVIDDD 195