BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0014.p (517 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776820.1| 2-isopropylmalate synthase [Herbaspirillum s... 952 0.0 ref|YP_004752177.1| 2-isopropylmalate synthase [Collimonas fungi... 907 0.0 ref|YP_001352888.1| 2-isopropylmalate synthase [Janthinobacteriu... 833 0.0 emb|CAL61215.2| 2-isopropylmalate synthase (Alpha-isopropylmalat... 827 0.0 ref|YP_004418226.1| 2-isopropylmalate synthase [Pusillimonas sp.... 778 0.0 >ref|YP_003776820.1| 2-isopropylmalate synthase [Herbaspirillum seropedicae SmR1] gb|ADJ64912.1| 2-isopropylmalate synthase protein [Herbaspirillum seropedicae SmR1] Length = 567 Score = 952 bits (2461), Expect = 0.0, Method: Compositional matrix adjust. Identities = 453/509 (88%), Positives = 479/509 (94%) Query: 1 MLNNPAAKYRAFPTIPLSDRTWPNQFISKPPIWMSTDLRDGNQALIEPMSPERKLRFFEM 60 ML+NPAAKYR FP I L DRTWP++ I+ PPIWMSTDLRDGNQALIEPM+ ERKLRFFE+ Sbjct: 2 MLSNPAAKYRPFPAIDLPDRTWPSKVINTPPIWMSTDLRDGNQALIEPMNAERKLRFFEL 61 Query: 61 LLQIGLKEIEVGFPSASQTDFDFVRKLIEENRIPDDVTIIVLTQSREELIRRTIDSLAGA 120 LL+ GLKEIEVGFPSASQTDFDFVRKLI ENRIPDDVTIIVLTQSREELIRRTI+SL GA Sbjct: 62 LLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSREELIRRTIESLEGA 121 Query: 121 KQAIVHLYNSVAPAFRKIVFNMSRDEIKNIAVTGTQLVKELTDARPETQWRYEYSPESFS 180 K+AIVHLYNSVAPAFRKIVFNMSR+EIKNIAVTGT+LVKELTDARP T+WR+EYSPESFS Sbjct: 122 KKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESFS 181 Query: 181 TTELDFSKEICDAVCETWGATPERKVILNLPSTVECSTPNVYADQIEWMCRNLKHRSSSI 240 TTELDFSKEICDAVCETWGA+PERKVILNLPSTVEC+TPNVYADQIEWMCRNLK R+S+I Sbjct: 182 TTELDFSKEICDAVCETWGASPERKVILNLPSTVECATPNVYADQIEWMCRNLKDRASTI 241 Query: 241 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 300 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD Sbjct: 242 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 301 Query: 301 FSDIDTVRQCVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQADAIWEVPYL 360 FSDID VRQ VEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQ DAIWEVPYL Sbjct: 302 FSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPYL 361 Query: 361 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADASGKE 420 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADA+GKE Sbjct: 362 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGKE 421 Query: 421 IVATDIYTIFKTEYLDRSTPYVYRAHRMSEDTSHEESIKIEVDIERNGEKTTVRGTGNGP 480 I A+DI+ IF+ EYL+R PYVYRAHRMSED+S ESI IEVDI RNG+ TVRG+GNGP Sbjct: 422 IAASDIHAIFQREYLERVEPYVYRAHRMSEDSSKAESINIEVDIVRNGQPVTVRGSGNGP 481 Query: 481 IDAFVNALGLDIKLMDFHEHAIGAAPTRK 509 IDAFV+ALGLDIKLMDFHEHAIGA K Sbjct: 482 IDAFVHALGLDIKLMDFHEHAIGAGADAK 510 >ref|YP_004752177.1| 2-isopropylmalate synthase [Collimonas fungivorans Ter331] gb|AEK61354.1| 2-isopropylmalate synthase [Collimonas fungivorans Ter331] Length = 568 Score = 907 bits (2343), Expect = 0.0, Method: Compositional matrix adjust. Identities = 422/505 (83%), Positives = 461/505 (91%) Query: 1 MLNNPAAKYRAFPTIPLSDRTWPNQFISKPPIWMSTDLRDGNQALIEPMSPERKLRFFEM 60 ML+NPA KYR FP IPLSDRTWPNQ IS PPIWMSTDLRDGNQALIEPM +RKLRFF++ Sbjct: 2 MLHNPAVKYRPFPPIPLSDRTWPNQVISAPPIWMSTDLRDGNQALIEPMDADRKLRFFKL 61 Query: 61 LLQIGLKEIEVGFPSASQTDFDFVRKLIEENRIPDDVTIIVLTQSREELIRRTIDSLAGA 120 L++IGLKEIEVGFPS SQTDFDFVRKLIEEN IPDDVTIIVLTQSRE+LIRRT+DSL A Sbjct: 62 LVKIGLKEIEVGFPSGSQTDFDFVRKLIEENHIPDDVTIIVLTQSREDLIRRTVDSLQNA 121 Query: 121 KQAIVHLYNSVAPAFRKIVFNMSRDEIKNIAVTGTQLVKELTDARPETQWRYEYSPESFS 180 KQAIVHLYN +APA+R+IVF+ SRDE+K IAV+GT+L+KELTDARPETQWRYEYSPE+FS Sbjct: 122 KQAIVHLYNPIAPAWRRIVFDKSRDEVKEIAVSGTRLIKELTDARPETQWRYEYSPETFS 181 Query: 181 TTELDFSKEICDAVCETWGATPERKVILNLPSTVECSTPNVYADQIEWMCRNLKHRSSSI 240 ELDFSKE+CDAV E W A+P RKVILNLP+TVECSTPNV+ADQIEWM RNL R ++I Sbjct: 182 MAELDFSKEVCDAVVEAWHASPTRKVILNLPTTVECSTPNVFADQIEWMHRNLARRDATI 241 Query: 241 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 300 ISVHPHNDRGT VA+AELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD Sbjct: 242 ISVHPHNDRGTGVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 301 Query: 301 FSDIDTVRQCVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQADAIWEVPYL 360 FSDID VRQCVE+CNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFA Q+ADAIWE+PYL Sbjct: 302 FSDIDEVRQCVEDCNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAAQKADAIWEIPYL 361 Query: 361 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADASGKE 420 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLA+PRRLQIEFSRAIQ+ ADA+GKE Sbjct: 362 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLAMPRRLQIEFSRAIQKAADATGKE 421 Query: 421 IVATDIYTIFKTEYLDRSTPYVYRAHRMSEDTSHEESIKIEVDIERNGEKTTVRGTGNGP 480 I A+DIY IFK EYLD+ TPYVYR HRMSED+S + ++IE+DI R+G+ T G GNGP Sbjct: 422 IAASDIYNIFKQEYLDKQTPYVYRGHRMSEDSSQAQPVRIEIDIVRDGQAHTSSGHGNGP 481 Query: 481 IDAFVNALGLDIKLMDFHEHAIGAA 505 IDAFVNALGLDIKLMDFHEHAI A Sbjct: 482 IDAFVNALGLDIKLMDFHEHAISAG 506 >ref|YP_001352888.1| 2-isopropylmalate synthase [Janthinobacterium sp. Marseille] gb|ABR88384.1| 2-isopropylmalate synthase [Janthinobacterium sp. Marseille] Length = 552 Score = 833 bits (2152), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/500 (76%), Positives = 445/500 (89%), Gaps = 2/500 (0%) Query: 1 MLNNPAAKYRAFPTIPLSDRTWPNQFISKPPIWMSTDLRDGNQALIEPMSPERKLRFFEM 60 ML NPAAKYRAFP + L DRTWPN+ IS PPIWMSTDLRDGNQALIEPM+ E+KLRFF+M Sbjct: 2 MLTNPAAKYRAFPPVQLKDRTWPNRVISTPPIWMSTDLRDGNQALIEPMNHEKKLRFFDM 61 Query: 61 LLQIGLKEIEVGFPSASQTDFDFVRKLIEENRIPDDVTIIVLTQSREELIRRTIDSLAGA 120 LL+IGLKEIEV FPSASQTDFDFVRKLI+E RIPDDVTI VLTQ+RE+LIRRT+DS+ G+ Sbjct: 62 LLKIGLKEIEVAFPSASQTDFDFVRKLIDEKRIPDDVTIEVLTQAREDLIRRTVDSVVGS 121 Query: 121 KQAIVHLYNSVAPAFRKIVFNMSRDEIKNIAVTGTQLVKELTDARPETQWRYEYSPESFS 180 ++AIVH+YN+ APAFR+IVFNMS++E+K +AV GT+L++ELTDA PET+W ++YSPE+FS Sbjct: 122 RRAIVHMYNATAPAFRRIVFNMSKEEVKALAVAGTKLIRELTDAHPETEWVFQYSPETFS 181 Query: 181 TTELDFSKEICDAVCETWGATPERKVILNLPSTVECSTPNVYADQIEWMCRNLKHRSSSI 240 TELDF+KE+CDAV E W TPERK+I+NLP TVE STPNVYADQ+EWM RNL R S I Sbjct: 182 MTELDFAKEVCDAVSEAWQPTPERKMIINLPGTVEASTPNVYADQVEWMHRNLARRDSII 241 Query: 241 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 300 +SVHPHNDRG AVA+AELAVMAGADR+EGCLFGNGERTGNVDLVTLALNLY+QG+NPGLD Sbjct: 242 LSVHPHNDRGCAVAAAELAVMAGADRIEGCLFGNGERTGNVDLVTLALNLYSQGINPGLD 301 Query: 301 FSDIDTVRQCVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQADAIWEVPYL 360 FSDID +RQCVEECNQIPVHPRHPYVGDLV+TAFSGSHQDAIKKGFAKQ+ D IWEVPY+ Sbjct: 302 FSDIDEIRQCVEECNQIPVHPRHPYVGDLVYTAFSGSHQDAIKKGFAKQKPDEIWEVPYM 361 Query: 361 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADASGKE 420 P+DPADLGRSYDAVIRVNSQSGKGGM YLLEQEYGL LPRRLQIEFSRA+QR D +G E Sbjct: 362 PVDPADLGRSYDAVIRVNSQSGKGGMTYLLEQEYGLVLPRRLQIEFSRAVQRITDETGGE 421 Query: 421 IVATDIYTIFKTEYLDRSTPYVYRAHRMSEDTSHEESIKIEVDIERNGEKTTVRGTGNGP 480 + A DI+TIF +EYL+ TPY YR H + D S + ++I+V++E++G+ +++G GNGP Sbjct: 422 VAANDIHTIFNSEYLNAVTPYHYRNHHI--DDSKDGEVQIKVELEKDGKPVSMQGVGNGP 479 Query: 481 IDAFVNALGLDIKLMDFHEH 500 IDAF+NALGL+IKLMDFHEH Sbjct: 480 IDAFINALGLNIKLMDFHEH 499 >emb|CAL61215.2| 2-isopropylmalate synthase (Alpha-isopropylmalate synthase) (Alpha-IPM synthetase) [Herminiimonas arsenicoxydans] Length = 553 Score = 827 bits (2137), Expect = 0.0, Method: Compositional matrix adjust. Identities = 380/500 (76%), Positives = 441/500 (88%), Gaps = 1/500 (0%) Query: 1 MLNNPAAKYRAFPTIPLSDRTWPNQFISKPPIWMSTDLRDGNQALIEPMSPERKLRFFEM 60 ML+NP KYRAFP + L+DR WPNQ ISK PIWMSTDLRDGNQ+LIEPM+ ++KLRFF+M Sbjct: 2 MLSNPEQKYRAFPPVRLTDRNWPNQVISKAPIWMSTDLRDGNQSLIEPMNHDKKLRFFDM 61 Query: 61 LLQIGLKEIEVGFPSASQTDFDFVRKLIEENRIPDDVTIIVLTQSREELIRRTIDSLAGA 120 L++IGLKEIEV FPSASQTDFDFVR+LIEE RIPDDVTI VLT +RE+LIRRT++S+ GA Sbjct: 62 LVKIGLKEIEVAFPSASQTDFDFVRRLIEEKRIPDDVTIEVLTPAREDLIRRTVESVVGA 121 Query: 121 KQAIVHLYNSVAPAFRKIVFNMSRDEIKNIAVTGTQLVKELTDARPETQWRYEYSPESFS 180 ++AI+HLYN+ APAFR+IVFNMSRDE+K +AV G +LV+ELTDA PET+W ++YSPE+FS Sbjct: 122 RRAIIHLYNATAPAFRRIVFNMSRDEVKALAVAGAKLVRELTDAHPETEWVFQYSPEAFS 181 Query: 181 TTELDFSKEICDAVCETWGATPERKVILNLPSTVECSTPNVYADQIEWMCRNLKHRSSSI 240 TEL+FSKEICDAV TW TPERK+I NLP TVE STPNVYADQ+EWM RNL R S + Sbjct: 182 MTELEFSKEICDAVSATWQPTPERKMIFNLPGTVEASTPNVYADQVEWMHRNLARRDSIL 241 Query: 241 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 300 ISVHPHNDRG AVA+AELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLY+QG++PGLD Sbjct: 242 ISVHPHNDRGCAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYSQGISPGLD 301 Query: 301 FSDIDTVRQCVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQADAIWEVPYL 360 FSDID VRQCVEECNQIPVHPRHPYVGDLV+TAFSGSHQDAIKKGFA+QQ DAIWEVPY+ Sbjct: 302 FSDIDAVRQCVEECNQIPVHPRHPYVGDLVYTAFSGSHQDAIKKGFAQQQPDAIWEVPYM 361 Query: 361 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADASGKE 420 P+DPADLGRSYDAVIRVNSQSGKGGM YLLEQEYGL LPRRLQIEFSRA+QR D +G E Sbjct: 362 PVDPADLGRSYDAVIRVNSQSGKGGMTYLLEQEYGLVLPRRLQIEFSRAVQRITDETGGE 421 Query: 421 IVATDIYTIFKTEYLDRSTPYVYRAHRMSEDTSHEESIKIEVDIERNGEKTTVRGTGNGP 480 + A DI+TIF EYL+ TPY YR+H + E + E ++I V++E++G+ T++G GNGP Sbjct: 422 VAANDIHTIFNNEYLNAVTPYQYRSHHIDESQGNAE-VRIRVELEKDGKPVTLQGAGNGP 480 Query: 481 IDAFVNALGLDIKLMDFHEH 500 IDAF+NALG+DI+LMDFHEH Sbjct: 481 IDAFINALGMDIRLMDFHEH 500 >ref|YP_004418226.1| 2-isopropylmalate synthase [Pusillimonas sp. T7-7] gb|AEC21602.1| 2-isopropylmalate synthase [Pusillimonas sp. T7-7] Length = 567 Score = 778 bits (2010), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/509 (71%), Positives = 428/509 (84%), Gaps = 4/509 (0%) Query: 1 MLNNPAAKYRAFPTI--PLSDRTWPNQFISKPPIWMSTDLRDGNQALIEPMSPERKLRFF 58 M++NP+ KYR F S+RTWP++ I+ PPIWMSTDLRDGNQ+LIEPMS ERKLRFF Sbjct: 1 MISNPSDKYRPFVAFNRDFSERTWPSKRITHPPIWMSTDLRDGNQSLIEPMSVERKLRFF 60 Query: 59 EMLLQIGLKEIEVGFPSASQTDFDFVRKLIEENRIPDDVTIIVLTQSREELIRRTIDSLA 118 E L++IG KEIEVGFPSASQTDFDFVRKL++ENRIPDDVTII LTQSRE+LI+RT++S A Sbjct: 61 EQLIKIGFKEIEVGFPSASQTDFDFVRKLVDENRIPDDVTIIALTQSREDLIQRTVESAA 120 Query: 119 GAKQAIVHLYNSVAPAFRKIVFNMSRDEIKNIAVTGTQLVKELTDARPETQWRYEYSPES 178 GAKQAI+HLYN+ APAFRKIVFNMS+DE++ IA+TGT+LVKE T PET WRYEYSPE Sbjct: 121 GAKQAIIHLYNACAPAFRKIVFNMSKDEVREIALTGTRLVKEYTSRHPETSWRYEYSPEV 180 Query: 179 FSTTELDFSKEICDAVCETWGATPERKVILNLPSTVECSTPNVYADQIEWMCRNLKHRSS 238 FSTTE +F+ E+C+AV E W TPE K+I NLP+T+E +TPN+YADQIEWM NLK R Sbjct: 181 FSTTEPEFALEVCNAVTEVWQPTPESKIIYNLPATIEATTPNMYADQIEWMHNNLKQRDC 240 Query: 239 SIISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPG 298 ++SVHPHNDRGTAVA+AELAVMAGADR+EGCLFGNGERTGNVDLVTLALNLYTQG++PG Sbjct: 241 IVLSVHPHNDRGTAVAAAELAVMAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIHPG 300 Query: 299 LDFSDIDTVRQCVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQADAIWEVP 358 LDFSDID VR+CVE CNQ+PVHPRHPY GDLVFTAFSGSHQDAIKKG A+QQADA+WEVP Sbjct: 301 LDFSDIDEVRRCVEYCNQLPVHPRHPYAGDLVFTAFSGSHQDAIKKGLARQQADALWEVP 360 Query: 359 YLPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADASG 418 YLPIDPADLGRSYDAVIRVNSQSGKGG++YLLE E+GL LPRRLQIEFSRAIQR D +G Sbjct: 361 YLPIDPADLGRSYDAVIRVNSQSGKGGVSYLLESEHGLVLPRRLQIEFSRAIQRVTDETG 420 Query: 419 KEIVATDIYTIFKTEYLDRSTPYVYRAHRMSED--TSHEESIKIEVDIERNGEKTTVRGT 476 E+ + +Y +F TEYL + P+ HR++ D ++ E IEV++E NG + G Sbjct: 421 AEVTTSQVYELFSTEYLQQEQPWKLVRHRITGDPKAANGEQFMIEVELEENGVVRNLVGR 480 Query: 477 GNGPIDAFVNALGLDIKLMDFHEHAIGAA 505 G+G I AFVNAL ++I++MD+HEHAIG Sbjct: 481 GDGAISAFVNALDMNIRVMDYHEHAIGTG 509