BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0170.p (517 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08628877.1| long-chain-fatty-acid--CoA ligase [Bradyrhizo... 698 0.0 ref|YP_002825152.1| long-chain-fatty-acid--CoA ligase [Sinorhizo... 614 e-174 gb|AEJ26833.1| Long-chain-fatty-acid--CoA ligase [Paracoccus den... 546 e-153 ref|YP_004336175.1| Long-chain-fatty-acid--CoA ligase [Pseudonoc... 485 e-135 ref|YP_001509110.1| beta-ketoacyl synthase [Frankia sp. EAN1pec]... 476 e-132 >ref|ZP_08628877.1| long-chain-fatty-acid--CoA ligase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP08236.1| long-chain-fatty-acid--CoA ligase [Bradyrhizobiaceae bacterium SG-6C] Length = 521 Score = 698 bits (1801), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/515 (64%), Positives = 405/515 (78%), Gaps = 2/515 (0%) Query: 1 MIHCELIAPVGELIRRHAQRTPRKIAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDS 60 M+H +LIAPV L+RRHA+ KIA+ D T+V+Y +L T NLAGHL D G+ GD Sbjct: 1 MLHTDLIAPVPALLRRHAEARGSKIAFRDAETAVTYADLAARTANLAGHLVDHGVKPGDK 60 Query: 61 VAIFLPNSVDWVESCFAIVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTDDKADVVA 120 VAIFLPNSV W+ESCFA+ RA V VP+SY+AT EV YR++DAGC I TTD++ D++A Sbjct: 61 VAIFLPNSVAWIESCFAVNRADAVGVPVSYEATASEVLYRLQDAGCTTIITTDERMDMIA 120 Query: 121 ALQARFPFLKTVFLTEGASKAGGKDGLRYLDLRSAAAKSLPREAEDIDQSSFIIYTSGTT 180 A++A P L + L + + + GLRY + + KS P ++ DI +FIIYTSGTT Sbjct: 121 AVRADAPSLNLIVLVDRGPET--RQGLRYSEFAAGKPKSAPPDSLDIHAPAFIIYTSGTT 178 Query: 181 GRAKGVLLTQRSMLWVCAACWAPIANLNENDRVLSPLPLFHSYALNLSVLSILAVGASEY 240 GRAKGVLL+ SMLWV AACWAPIA L+E D VLSPLPLFHSYALNLSVL ILA GA+EY Sbjct: 179 GRAKGVLLSVHSMLWVVAACWAPIAGLSEADTVLSPLPLFHSYALNLSVLGILATGATEY 238 Query: 241 IMDKFSTSETLALLKSGDFTVFPAVPTIFHYLLEGARADPGCKFPGLRLCLSAGAIMPAP 300 +M+K+ST+E LLK+G+FTVFP VPT+FHY LE A+++P FP LR+ +SAGAIMPA Sbjct: 239 VMEKYSTTEAARLLKTGEFTVFPGVPTMFHYFLEAAKSEPAQAFPKLRIGMSAGAIMPAT 298 Query: 301 INKEFEERFGVMLLDGYGITETSTMVTLNWQHGTRVMGSCGLPLPGLATRIVDPSTGLDV 360 +N+EFE RFG+ LLDGYGITETSTMVT+NW G R+MGSCGLPLPGLA RIV P +G DV Sbjct: 299 LNREFESRFGIKLLDGYGITETSTMVTMNWMTGARIMGSCGLPLPGLAVRIVSPVSGDDV 358 Query: 361 ESGQEGELIVRGPNLMKGYHRKPEETAKALQNGWYYTGDLARSDQSGYLTITGRLKELII 420 G+EGELIVRGPNLM GYH KP+ETA AL+NGWY+TGDLA+SD++G+LTITGRLKELII Sbjct: 359 AIGEEGELIVRGPNLMSGYHNKPQETAAALRNGWYHTGDLAKSDENGFLTITGRLKELII 418 Query: 421 RGGQNIAPAEVEEAVSAFEAVLDCAVVGAPHQHLGEVPVVFVVSRPGMTVVEADLLLHCK 480 RGGQNIAPAE+EEAV++ E V+D AVVG PHQHLGEVP +FVV+R G + +L HC+ Sbjct: 419 RGGQNIAPAEIEEAVNSLEDVIDSAVVGIPHQHLGEVPALFVVTRGGGRIDAEAVLEHCR 478 Query: 481 ERLSSYKLPHAVHVVAEIPRTGSGKIIRYKLRETL 515 RLSSYK+P +VH V +IPRTGSGKIIR+KLRE L Sbjct: 479 GRLSSYKVPFSVHFVTDIPRTGSGKIIRFKLREML 513 >ref|YP_002825152.1| long-chain-fatty-acid--CoA ligase [Sinorhizobium fredii NGR234] gb|ACP24399.1| long-chain-fatty-acid--CoA ligase [Sinorhizobium fredii NGR234] Length = 508 Score = 614 bits (1584), Expect = e-174, Method: Compositional matrix adjust. Identities = 312/518 (60%), Positives = 382/518 (73%), Gaps = 12/518 (2%) Query: 1 MIHCELIAPVGELIRRHAQRTPRKIAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDS 60 MIH ELIAPVG ++RR+A+ P K+A+EDR SVSY L++ T +LA L G+ G + Sbjct: 1 MIHFELIAPVGAILRRNARLYPGKMAFEDRRRSVSYDALDRETEDLASRLVALGLRKGQA 60 Query: 61 VAIFLPNSVDWVESCFAIVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTDDKADVVA 120 VAIFLPNSV+WV +C A+VRAGG AVP+S +ATE E+AYRI DAGC + +K VV Sbjct: 61 VAIFLPNSVNWVVACLAVVRAGGTAVPVSIEATEAEIAYRISDAGCAFLIMPPEKQPVVD 120 Query: 121 ALQARFPFLKTVFLTEGASKAGGKDGLRYLDLRSAAAKSLPREAEDIDQSSFIIYTSGTT 180 L R V A++ G D R+AA + + +DID+ +FI+YTSGTT Sbjct: 121 TLLQRSDKPPVVI---AANEEIGFDA----SGRTAAFE----DDQDIDRPAFIVYTSGTT 169 Query: 181 GRAKGVLLTQRSMLWVCAACWAPIANLNENDRVLSPLPLFHSYALNLSVLSILAVGASEY 240 G+ KGVLL+ RSMLWV AACWAPIA LNEND VL+ LPL+HSYALN++VLSI+A GASEY Sbjct: 170 GQPKGVLLSMRSMLWVTAACWAPIAGLNENDIVLNTLPLYHSYALNIAVLSIIATGASEY 229 Query: 241 IMDKFSTSETLALLKSGDFTVFPAVPTIFHYLLEGARADPGCKFPGLRLCLSAGAIMPAP 300 IM+KFSTS+ LL++G FT P VPT+FHY L +A +RLC+SAGAIMP Sbjct: 230 IMEKFSTSQATELLRTGRFTFMPGVPTVFHYFLSACQASGERLLSSVRLCVSAGAIMPGT 289 Query: 301 INKEFEERFGVMLLDGYGITETSTMVTLNWQHGTRVMGSCGLPLPGLATRIVDPSTGLDV 360 +N +FE F V LLDGYGITETSTMVT+NW RV GSCGLP+PGL R+VDP TGLDV Sbjct: 290 LNSDFEAFFDVPLLDGYGITETSTMVTMNWPGKWRVPGSCGLPVPGLTVRLVDPKTGLDV 349 Query: 361 ESGQEGELIVRGPNLMKGYHRKPEETAKALQNGWYYTGDLARSDQSGYLTITGRLKELII 420 +G EGELI +GPNLM+GYH KP ET KA+ +GWY+TGDLA+SD +G+LTITGRLKELII Sbjct: 350 PAGVEGELICKGPNLMQGYHNKPLETEKAVVDGWYHTGDLAKSDLNGFLTITGRLKELII 409 Query: 421 RGGQNIAPAEVEEAVSAF-EAVLDCAVVGAPHQHLGEVPVVFVVSRPGMTVVEADLLLHC 479 RGGQNIAPAEVEEA+ + V+DCAVVG PH LGEVPV +V + G + DL+ HC Sbjct: 410 RGGQNIAPAEVEEAILLLHDTVMDCAVVGMPHSMLGEVPVACIVVKEGQAFDQEDLMRHC 469 Query: 480 KERLSSYKLPHAVHVVAEIPRTGSGKIIRYKLRETLPS 517 K RLS+YK+P V+VVAEIPRTGSGKI+R KLRE+LP+ Sbjct: 470 KARLSAYKVPDRVYVVAEIPRTGSGKILRVKLRESLPA 507 >gb|AEJ26833.1| Long-chain-fatty-acid--CoA ligase [Paracoccus denitrificans SD1] Length = 505 Score = 546 bits (1407), Expect = e-153, Method: Compositional matrix adjust. Identities = 284/513 (55%), Positives = 360/513 (70%), Gaps = 14/513 (2%) Query: 1 MIHCELIAPVGELIRRHAQRTPRKIAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDS 60 MIH +LI +G L+ RHA+ TP K AY D ++++ L+ TT +A L G+ G+S Sbjct: 1 MIHLDLIETIGTLMARHAEATPDKPAYIDDDRTITWTGLQSETTAIAADLLAGGLQQGES 60 Query: 61 VAIFLPNSVDWVESCFAIVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTDDKADVVA 120 VAI+L NSVDWV + A+VRAGG+AVPIS +AT HE AYRI+D AIF V+ Sbjct: 61 VAIWLANSVDWVVATLAVVRAGGIAVPISAEATAHEAAYRIQDCAAAAIFAEASLRPVLD 120 Query: 121 ALQARFPFLKTVFLTEGASKAGGKDGLRYLDLRSAAAKSLPREAEDIDQSSFIIYTSGTT 180 + + L + +G L + ++ +LP E +I +F++YTSGTT Sbjct: 121 EVAGQGIALPRLIWRDGG-----------LAVPASTEVALPDE--NIHAPAFVVYTSGTT 167 Query: 181 GRAKGVLLTQRSMLWVCAACWAPIANLNENDRVLSPLPLFHSYALNLSVLSILAVGASEY 240 G+ KGV+LT SMLWV AACW PIA L+ +D VLSPLPLFHSYALN++VL ILA GA+E Sbjct: 168 GKPKGVVLTVHSMLWVSAACWTPIAGLSADDVVLSPLPLFHSYALNIAVLGILATGATER 227 Query: 241 IMDKFSTSETLALLKSGDFTVFPAVPTIFHYLLEGARADPGCKFPGLRLCLSAGAIMPAP 300 IM +FS S L LL++G+ TV P VPT+FHYLL AR LR+CLSAGAI+PA Sbjct: 228 IMARFSASRALELLQAGEATVLPGVPTMFHYLLLAAREKGVDSLGKLRICLSAGAILPAA 287 Query: 301 INKEFEERFGVMLLDGYGITETSTMVTLNWQHGTRVMGSCGLPLPGLATRIVDPSTGLDV 360 +N++FE FGV LLDGYGITETSTMVT+N R+ GSCGLPLPG+A RI+D ++GLD Sbjct: 288 LNRDFEAHFGVELLDGYGITETSTMVTMNRPGRYRIPGSCGLPLPGVAVRIIDSASGLDQ 347 Query: 361 ESGQEGELIVRGPNLMKGYHRKPEETAKALQNGWYYTGDLARSDQSGYLTITGRLKELII 420 G EGELI GPN+M+GY KP+ET KAL+NGWY+TGDLARSD++G+LTITGRLKE++I Sbjct: 348 PRGDEGELICSGPNVMQGYLNKPDETGKALRNGWYHTGDLARSDENGFLTITGRLKEVVI 407 Query: 421 RGGQNIAPAEVEEAVSAFEAVLDCAVVGAPHQHLGEVPVVFVVSRPGMTVVEADLLLHCK 480 RGGQNI+P EVEEA+ AV DCAVVG PH+ LGEVPV +VV +A +L+HC+ Sbjct: 408 RGGQNISPGEVEEALIEHSAVRDCAVVGVPHETLGEVPVAYVVWETPAAETDA-VLIHCR 466 Query: 481 ERLSSYKLPHAVHVVAEIPRTGSGKIIRYKLRE 513 L+ YKLPHA+ + IPRTGSGKIIR+KLRE Sbjct: 467 TLLAPYKLPHAIRTIDTIPRTGSGKIIRFKLRE 499 >ref|YP_004336175.1| Long-chain-fatty-acid--CoA ligase [Pseudonocardia dioxanivorans CB1190] gb|AEA28322.1| Long-chain-fatty-acid--CoA ligase [Pseudonocardia dioxanivorans CB1190] Length = 509 Score = 485 bits (1248), Expect = e-135, Method: Compositional matrix adjust. Identities = 263/519 (50%), Positives = 340/519 (65%), Gaps = 19/519 (3%) Query: 1 MIHCELIAPVGELIRRHAQRTPRKIAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDS 60 M+ +LI P+ +L+ HA+ P A+ D + Y L + T LAG+LQ G+ GD Sbjct: 1 MLRTDLIRPLPDLLAEHARVRPDVTAFVDDRRADDYATLARNTAALAGNLQRLGLDPGDR 60 Query: 61 VAIFLPNSVDWVESCFAIVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTDDKADVVA 120 V ++L NSV+ VE+ A+ RAGG+AV + + EVA+ + D+G + + T D A V Sbjct: 61 VLLYLDNSVETVEAYLAVPRAGGIAVCANPGSAAPEVAHVLADSGARFVIT--DAAHVDT 118 Query: 121 ALQARFPFLKTVFLTEGASKAGGKDGLRYLDLRSAAAKSLPREAEDIDQSSFIIYTSGTT 180 A ++ + + E L D+R PR+ +D ++++YTSGTT Sbjct: 119 ARTLVGEDVRLIVVGESYEAL-----LEPTDVR-------PRDGLALDDPAWMLYTSGTT 166 Query: 181 GRAKGVLLTQRSMLWVCAACWAPIANLNENDRVLSPLPLFHSYALNLSVLSILAVGASEY 240 GR KGV LTQR LWV AAC+API + D +LSPLPLFHSYAL L VL++ A GASE Sbjct: 167 GRPKGVYLTQRGCLWVVAACFAPILGVGPGDVLLSPLPLFHSYALVLCVLAVAATGASER 226 Query: 241 IMDKFSTSETLALLKS----GDFTVFPAVPTIFHYLLEGARADPGCKFPGLRLCLSAGAI 296 ++ +FS L L+ G TVFP VPT+FHYLL+ AR D G LR+C+SAGAI Sbjct: 227 LLPRFSVESVLRRLREPGPEGPVTVFPGVPTMFHYLLDRAR-DSGLGATDLRVCMSAGAI 285 Query: 297 MPAPINKEFEERFGVMLLDGYGITETSTMVTLNWQHGTRVMGSCGLPLPGLATRIVDPST 356 MPA +N EFE FGV LLDGYGITETSTMVT+NW GTRVMGSCGLPLPGL R+VDP+T Sbjct: 286 MPAALNAEFEATFGVPLLDGYGITETSTMVTMNWPTGTRVMGSCGLPLPGLTVRLVDPAT 345 Query: 357 GLDVESGQEGELIVRGPNLMKGYHRKPEETAKALQNGWYYTGDLARSDQSGYLTITGRLK 416 G DV G+EGEL V+GPN+ GYH P+ TA L +GWY TGDL R D+ GYL I+GR K Sbjct: 346 GRDVLPGEEGELWVQGPNVTPGYHELPDATAAVLVDGWYRTGDLGRRDEHGYLRISGRTK 405 Query: 417 ELIIRGGQNIAPAEVEEAVSAFEAVLDCAVVGAPHQHLGEVPVVFVVSRPGMTVVEADLL 476 E+IIRGG+N+ PAEVE+A+ A + V D AVVGAPH LGEVPV FVV R +L Sbjct: 406 EMIIRGGENVYPAEVEDALLASDEVADAAVVGAPHAGLGEVPVGFVVPRDPAAFDAEKVL 465 Query: 477 LHCKERLSSYKLPHAVHVVAEIPRTGSGKIIRYKLRETL 515 C++RLSS+K+P + V A+IPRTGSGKI+R++L++ L Sbjct: 466 AVCRDRLSSFKVPDRLVVTADIPRTGSGKILRFRLQQRL 504 >ref|YP_001509110.1| beta-ketoacyl synthase [Frankia sp. EAN1pec] gb|ABW14204.1| Beta-ketoacyl synthase [Frankia sp. EAN1pec] Length = 4930 Score = 476 bits (1226), Expect = e-132, Method: Compositional matrix adjust. Identities = 256/515 (49%), Positives = 328/515 (63%), Gaps = 5/515 (0%) Query: 1 MIHCELIAPVGELIRRHAQRTPRKIAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDS 60 M+ ELIAP+ +L+R +A+R +IA+ D +V + ELE+ T LAGHL D + GD Sbjct: 3 MLRTELIAPLPKLLRGNAERLGDRIAFRDASRAVGWAELERRTGWLAGHLADLRLQPGDR 62 Query: 61 VAIFLPNSVDWVESCFAIVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTDDKADVVA 120 AI L N V+ VES RA V VPI+ TE E+AY ++D G + + T + D+V Sbjct: 63 AAIVLGNCVEVVESYLGFARASVVGVPINPRVTETELAYLLDDCGARLVVTDPARIDMVG 122 Query: 121 ALQARFPFLKTVFLTEGASKAGGKDG-LRYLDLRSAAAKSLPREAEDIDQSSFIIYTSGT 179 + P L+ V A G L + L A +S R+ +D ++++YTSGT Sbjct: 123 RVLRDRPGLRVVVTGGHAPPPSAPAGTLSFAALAGAQPRSAARDDLGLDDVAWMLYTSGT 182 Query: 180 TGRAKGVLLTQRSMLWVCAACWAPIANLNENDRVLSPLPLFHSYALNLSVLSILAVGASE 239 TGR KGVL TQRS LW AAC+API L+E DRVL PLPLFHS + VL + AVGAS Sbjct: 183 TGRPKGVLSTQRSCLWSVAACYAPIPGLSEQDRVLWPLPLFHSLSHIACVLGVTAVGASA 242 Query: 240 YIMDKFSTSETLALLKSGDFTVFPAVPTIFHYLLEGARADPGCKFPGLRLCLSAGAIMPA 299 ++D F+ SE LA ++ T VPT++HYL+ AR + G P LR+CL GAI A Sbjct: 243 RLLDGFAASEVLAAIQEDGSTFLAGVPTMYHYLVRAAR-ESGFSAPSLRMCLVGGAITTA 301 Query: 300 PINKEFEERFGVMLLDGYGITETSTMVTLNWQHGTRVMGSCGLPLPGLATRIVDPSTGLD 359 + ++FEE FG LLD YG TET +T+NW G RV GSCGLP+PGL R+VDP TGLD Sbjct: 302 RLRRDFEEAFGAPLLDAYGSTETCGSITINWPTGARVEGSCGLPVPGLGVRLVDPETGLD 361 Query: 360 VESGQEGELIVRGPNLMKGYHRKPEETAKALQNGWYYTGDLARSDQSGYLTITGRLKELI 419 V +G EGE+ VRGPN+M GYH +PE TA AL++GWY TGDLAR D +GY TITGR+KELI Sbjct: 362 VGAGAEGEVWVRGPNVMVGYHNQPEATAAALRDGWYRTGDLARRDDAGYFTITGRIKELI 421 Query: 420 IRGGQNIAPAEVEEAVSAFEAVLDCAVVGAPHQHLGEVPVVFVVSRPGMTVVEAD-LLLH 478 IRGG+NI P EVEE + V D AVV PH LGEVPV F+V PG ++ D LL Sbjct: 422 IRGGENIHPGEVEEVLRGVPGVADVAVVARPHDLLGEVPVAFLV--PGPEGLDPDRLLAT 479 Query: 479 CKERLSSYKLPHAVHVVAEIPRTGSGKIIRYKLRE 513 C+ERLS +K+P ++ + IPRT SGKI R+ L E Sbjct: 480 CRERLSYFKVPEELYEIDRIPRTASGKITRHVLLE 514