BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0156.p (613 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08628166.1| long-chain-fatty-acid--CoA ligase [Bradyrhizo... 540 e-151 ref|YP_421388.1| feruloyl-CoA synthase [Magnetospirillum magneti... 519 e-145 ref|NP_767686.1| feruloyl-CoA synthase [Bradyrhizobium japonicum... 499 e-139 ref|YP_002798616.1| feruloyl-CoA synthase [Azotobacter vinelandi... 493 e-137 ref|YP_001242742.1| feruloyl-CoA synthase [Bradyrhizobium sp. BT... 492 e-136 >ref|ZP_08628166.1| long-chain-fatty-acid--CoA ligase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP09075.1| long-chain-fatty-acid--CoA ligase [Bradyrhizobiaceae bacterium SG-6C] Length = 619 Score = 540 bits (1392), Expect = e-151, Method: Compositional matrix adjust. Identities = 282/592 (47%), Positives = 379/592 (64%), Gaps = 4/592 (0%) Query: 12 FVSPPVIATPESGGIVLRSGLAPTEPFL-SLHEMLERAAELVPHNIFLQERSVFDRNLWN 70 F +P +IA ++ G + P +PF + + LE+ A P +FL ER+ D W Sbjct: 16 FAAPNIIADRKADGSIWLKSATPLQPFARCVGDWLEQWARETPDTVFLAERASADLP-WR 74 Query: 71 RVTYIEALQLVDQYGRALLNYGLTPDRPLAIISPNSINHALLALAAMSVGIPVAPISVAY 130 ++TY E ++ V +L G++ +RPL I+S NSI+HA+ LAAM VG+P AP+S AY Sbjct: 75 KLTYRETMRRVRAVASWILAQGMSAERPLVILSDNSIDHAIFMLAAMHVGVPAAPVSPAY 134 Query: 131 SQMA-DLERVENIFRLLTPGMVFAETWAGFERALSAAAKAGANILVGNSTGVDQPVLTLE 189 S M+ D ++++++ LL PG ++ + F AL+A A A I+V + + Sbjct: 135 SLMSKDYDKLKSMIALLDPGAIYVSSATMFATALNAIAPAHTAIVVAGDPDGFEGAIAFS 194 Query: 190 KIRDPGQAVGSIRARFPVGPDTIAKVLFTSGSTGSSKGALVTQRMMCSNQEALTQCWPFL 249 + + RA VG DTIAK LFTSGSTG+ K + TQRM+ SNQ A Q WPFL Sbjct: 195 SLTAAAETPDVARAFAGVGHDTIAKFLFTSGSTGAPKAVINTQRMLTSNQAAKGQTWPFL 254 Query: 250 EAEPPVAVDWLPWSHVFGGCLIFNCVLRHLGTMVIDDGRPVGSQFERTLQNIRELPPSIY 309 +DWLPWSH FG FNCVLR+ GT+ ID G+P F +L N+R++ P++Y Sbjct: 255 SETGFTTLDWLPWSHTFGANHNFNCVLRNGGTLYIDSGKPAPGLFALSLANLRDVIPTVY 314 Query: 310 LSVPKALDELVAALRKDDELCRRFFSRLRLIFSAGAGLPKSTWEALHELAGRWSPHEMRI 369 +VP+ D L+AALR+DD+L RRFFS ++L+F A A LP++ W+AL L+ H + + Sbjct: 315 FNVPRGYDMLIAALREDDDLRRRFFSDVKLLFYAAAALPQNLWDALEALSIATVGHAVPM 374 Query: 370 VISYGSTETAPVVCVSSGQSKRPDNIGVPIPGAEMKLAPSEDKFEIRLRGPMVTPGYWRH 429 V ++GSTETAP+ ++R NIGVP+PG E+KL P+ DK E R+RGP VTPGYW+ Sbjct: 375 VSAWGSTETAPLATDCHFLAQRSGNIGVPVPGTEIKLVPTGDKLEARVRGPNVTPGYWKA 434 Query: 430 PELAKDIFDEDGYYKMGDAGKLEDPSRPELGIVFDGRVAEDFKLVTGTWVRTTNVRIACV 489 PE FD DG+Y MGDA L D +RPELG+ FDGRVAEDFKL +GTWV +RIA + Sbjct: 435 PEQTAKAFDADGFYMMGDAMVLADSARPELGLFFDGRVAEDFKLTSGTWVSVGTLRIAGI 494 Query: 490 SALAPLIQDAVVTGQDRDEVGLLAFPSLNGCRQLTKLPGASLAELIDHPIIREAVAQALA 549 +ALAP+ QD VVTG DRDEV L FP++ CR L KLP A +A+++ P +REAVAQ LA Sbjct: 495 AALAPIAQDIVVTGHDRDEVRFLVFPNIAACRALAKLPDADVADVLRAPAVREAVAQGLA 554 Query: 550 RLN-QGAGSSGRIGRVLLMLTPPSIDTGEITDKGYINQRAVLRARANEVERL 600 RL G GSS R LL++ PS+D GEITDKGYINQRAVL RA ++ERL Sbjct: 555 RLKGNGGGSSSFATRALLLVELPSVDGGEITDKGYINQRAVLSRRAPDIERL 606 >ref|YP_421388.1| feruloyl-CoA synthase [Magnetospirillum magneticum AMB-1] dbj|BAE50829.1| Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II [Magnetospirillum magneticum AMB-1] Length = 644 Score = 519 bits (1336), Expect = e-145, Method: Compositional matrix adjust. Identities = 284/612 (46%), Positives = 390/612 (63%), Gaps = 15/612 (2%) Query: 1 MHAVAVTPSVNFVSPPV-IATPESGGIVLRSGLAPTEPFLSLHEMLERAAELVPHNIFLQ 59 M++VA P +NF V + E G ++RS + L ++L AE +P+ FL Sbjct: 33 MNSVAWAP-INFAPARVEVEKLEDGAFIIRSPDSLKPYSRCLGDLLVHWAERIPNQTFLA 91 Query: 60 ERSVFDRNLWNRVTYIEALQLVDQYGRALLNYGLTPDRPLAIISPNSINHALLALAAMSV 119 +R D + W RVT+ E L+ V+ G++LL+ GL +RP+ I+S NSI+HALLALAAM V Sbjct: 92 QR---DGDGWRRVTWAETLKKVEAIGQSLLDRGLNKNRPVMILSDNSIDHALLALAAMHV 148 Query: 120 GIPVAPISVAYSQMA-DLERVENIFRLLTPGMVFAETWAGFERALSAAAKAGANILVGNS 178 +PV+PIS AYS ++ D ++ I LL PG+V+A A F AL A ++ S Sbjct: 149 SVPVSPISPAYSLISQDHSKLRYIADLLKPGLVYASDGARFADALVIPELARVEVVT--S 206 Query: 179 TGVDQPVLTLEKI--RDPGQAVGSIRARFPVGPDTIAKVLFTSGSTGSSKGALVTQRMMC 236 T +T + + PG V + A V P T+AK+LFTSGST KG + TQ+M+C Sbjct: 207 TNPIACGVTFDTLLAATPGPEVENAFAN--VRPTTVAKILFTSGSTDMPKGVINTQKMLC 264 Query: 237 SNQEALTQCWPFLEAEPPVAVDWLPWSHVFGGCLIFNCVLRHLGTMVIDDGRPVGSQFER 296 SNQ+A+ Q WPFL PV VDWLPW+H FGG FN +LRH GT+ ID+G+P+ E+ Sbjct: 265 SNQQAIRQAWPFLLESHPVLVDWLPWNHTFGGNHNFNMMLRHGGTLYIDEGKPMPGLVEK 324 Query: 297 TLQNIRELPPSIYLSVPKALDELVAALRKDDELCRRFFSRLRLIFSAGAGLPKSTWEALH 356 T N++E+ P++Y +VP+ D L+ L KDDEL FF L++IF AGA LP++ WE + Sbjct: 325 TAANLKEVSPTLYFNVPRGFDMLIPMLEKDDELRNSFFKNLKIIFYAGAALPQNLWEKME 384 Query: 357 ELAGRWSPHEMRIVISYGSTETAPVVCVSSGQSKRPDNIGVPIPGAEMKLAPSEDKFEIR 416 +L+ R H++R+V S+GSTET+P++ R IG+P PG E+K+ P+ K E+R Sbjct: 385 QLSIRTLGHKVRMVSSWGSTETSPMITTVHFDIPRAGIIGLPAPGCEVKMIPNGGKLEMR 444 Query: 417 LRGPMVTPGYWRHPELAKDIFDEDGYYKMGDAGKLEDPSRPELGIVFDGRVAEDFKLVTG 476 ++GP +TPGY++ +L FDEDG+Y +GDAGKL DP P GIVFDGRVAEDFKL +G Sbjct: 445 IKGPNITPGYFKRADLTAKAFDEDGWYMIGDAGKLADPDDPSKGIVFDGRVAEDFKLTSG 504 Query: 477 TWVRTTNVRIACVSALAPLIQDAVVTGQDRDEVGLLAFPSLNGCRQLT--KLPGASLAEL 534 TWV +R++ + A AP++QDAV+TG DR+ VGLL F SL GC +L P L EL Sbjct: 505 TWVHVGALRLSVIDAAAPVLQDAVITGHDRESVGLLGFASLPGCLRLCPDAAPDTPLKEL 564 Query: 535 IDHPIIREAVAQALARL-NQGAGSSGRIGRVLLMLTPPSIDTGEITDKGYINQRAVLRAR 593 + P +R+ + A+ L +G G+S RI R LLM PPSID EITDKGYINQRAVL R Sbjct: 565 MKRPEVRDKLLGAMKALAAEGKGTSHRITRALLMDAPPSIDANEITDKGYINQRAVLTNR 624 Query: 594 ANEVERLYSPTD 605 + VE+LY+P D Sbjct: 625 VHLVEKLYAPGD 636 >ref|NP_767686.1| feruloyl-CoA synthase [Bradyrhizobium japonicum USDA 110] dbj|BAC46311.1| long-chain-fatty-acid-CoA ligase [Bradyrhizobium japonicum USDA 110] Length = 644 Score = 499 bits (1286), Expect = e-139, Method: Compositional matrix adjust. Identities = 280/596 (46%), Positives = 371/596 (62%), Gaps = 10/596 (1%) Query: 12 FVSPPVIATPES-GGIVLRSGLAPTEPFLSLHEMLERAAELVPHNIFLQERSVFDRNLWN 70 F +P IA + G IVLRS + + + LE+ A P IFL ER + W Sbjct: 39 FAAPKTIAEHRADGSIVLRSPEPLHDSARCIGDWLEQWARQTPDAIFLAERGNAEAP-WT 97 Query: 71 RVTYIEALQLVDQYGRALLNYGLTPDRPLAIISPNSINHALLALAAMSVGIPVAPISVAY 130 VTY +AL+ V +L+ GL+ +RPL I+S NSI+HALLALAA VG+P A IS AY Sbjct: 98 TVTYAQALRQVRGAASWILSQGLSAERPLVILSDNSIDHALLALAAQHVGVPSAAISPAY 157 Query: 131 SQMA-DLERVENIFRLLTPGMVFAETWAGFERALSAAAK-AGANILVGNSTGVDQPVLTL 188 S M+ D ++++++ LL PG ++ F AL+A A I+ GN G D L Sbjct: 158 SLMSRDFDKLKSMIALLEPGAIYVSATKPFAAALAAIEPLHKAQIISGN--GADADALAF 215 Query: 189 EKIRDPGQAVGSIRARFPVGPDTIAKVLFTSGSTGSSKGALVTQRMMCSNQEALTQCWPF 248 + + +A V PDTIAK LFTSGSTG+ K + TQRM+ S+Q+A Q W F Sbjct: 216 SAVAATPEIADVAKAFAAVTPDTIAKFLFTSGSTGTPKAVINTQRMLTSSQQAKAQTWTF 275 Query: 249 LE--AEPPVAVDWLPWSHVFGGCLIFNCVLRHLGTMVIDDGRPVGSQFERTLQNIRELPP 306 LE V +DWLPWSH FG FN VLR+ G++ ID G+P F +L N++ + P Sbjct: 276 LEQAGSDLVILDWLPWSHTFGANHNFNLVLRNGGSLYIDGGKPAPGLFATSLANLKSVMP 335 Query: 307 SIYLSVPKALDELVAALRKDDELCRRFFSRLRLIFSAGAGLPKSTWEALHELAGRWSPHE 366 ++Y +VP+ D L+AALR D+ L RRFFS ++ F AGA LP++ W+AL +L+ Sbjct: 336 TVYFNVPRGFDMLIAALRGDEALRRRFFSEVKFAFYAGAALPQNLWDALEQLSVETVGRP 395 Query: 367 MRIVISYGSTETAPVVCVSSGQSKRPDNIGVPIPGAEMKLAPSEDKFEIRLRGPMVTPGY 426 + +V ++GSTET+P+ ++R NIGVPIPG E+KL S DK E+R+RGP VTPGY Sbjct: 396 LPMVSAWGSTETSPLATDCHFLAERSGNIGVPIPGTELKLVTSGDKLEVRVRGPNVTPGY 455 Query: 427 WRHPELAKDIFDEDGYYKMGDAGKLEDPSRPELGIVFDGRVAEDFKLVTGTWVRTTNVRI 486 W+ PEL + FD++G+Y +GDA KL D +RPE G+ FDGRVAEDFKL +GTWV +R+ Sbjct: 456 WKAPELTRQAFDDEGFYLIGDAVKLADSARPERGLFFDGRVAEDFKLNSGTWVSVGTLRL 515 Query: 487 ACVSALAPLIQDAVVTGQDRDEVGLLAFPSLNGCRQLTKLP-GASLAELIDHPIIREAVA 545 A ++ALAPL QD VV G DEV L FP++ CR LP A + +++ H IR A+A Sbjct: 516 AGIAALAPLAQDIVVAGHGGDEVRFLVFPNILACRGHAGLPETADVNDVLAHDKIRGAIA 575 Query: 546 QALARL-NQGAGSSGRIGRVLLMLTPPSIDTGEITDKGYINQRAVLRARANEVERL 600 Q LA+L QGA SSG R LL+ PPS+D GEITDKGYINQRAVL RA V RL Sbjct: 576 QGLAKLKQQGANSSGHATRALLLAEPPSVDGGEITDKGYINQRAVLTRRAEAVARL 631 >ref|YP_002798616.1| feruloyl-CoA synthase [Azotobacter vinelandii DJ] gb|ACO77641.1| feruoyl-CoA synthetase [Azotobacter vinelandii DJ] Length = 626 Score = 493 bits (1269), Expect = e-137, Method: Compositional matrix adjust. Identities = 276/609 (45%), Positives = 361/609 (59%), Gaps = 9/609 (1%) Query: 10 VNFVSPPVIATPESGGIVLR--SGLAPTEPFLSLHEMLERAAELVPHNIFLQERSVFDRN 67 V SP V+ G + LR LAP L + LE A L P I + R D Sbjct: 20 VAIGSPGVVHETADGCLYLRPVEALAPLP--ARLLDRLEHWARLTPEQILVARRG--DDG 75 Query: 68 LWNRVTYIEALQLVDQYGRALLNYGLTPDRPLAIISPNSINHALLALAAMSVGIPVAPIS 127 W R++Y + L+ G+ LL YGL+ +RPL ++S N+++H LALA + VGIP P+S Sbjct: 76 EWQRLSYAQMLESARTIGQHLLEYGLSAERPLLVLSGNALDHLKLALACLYVGIPYCPVS 135 Query: 128 VAYSQMA-DLERVENIFRLLTPGMVFAETWAGFERALSAAAKAGANILVGNSTGVDQPVL 186 AYS ++ DL ++ +I L PG+VFA A + RA+ A +L +P Sbjct: 136 PAYSTLSQDLAKLRHIVERLQPGLVFAADGATYARAIEGTIPADVPLLFSEGVLPGRPCR 195 Query: 187 TLEKIRDPGQAVGSIRARFPVGPDTIAKVLFTSGSTGSSKGALVTQRMMCSNQEALTQCW 246 ++ P G+ A VGP++IAK LFTSGST K TQRM+C+NQ+ L Q + Sbjct: 196 AFAELERPVDTAGAEAAFARVGPESIAKFLFTSGSTKLPKAVPTTQRMLCANQQMLLQTF 255 Query: 247 PFLEAEPPVAVDWLPWSHVFGGCLIFNCVLRHLGTMVIDDGRPVGSQFERTLQNIRELPP 306 P EPPV VDWLPW+H FGG L + GT+ IDDG+P F TL+N+RE+ P Sbjct: 256 PVFAEEPPVLVDWLPWNHTFGGSHNVGIALYNGGTLYIDDGKPTPQGFAETLRNLREISP 315 Query: 307 SIYLSVPKALDELVAALRKDDELCRRFFSRLRLIFSAGAGLPKSTWEALHELAGRWSPHE 366 + YL+VPK +ELV AL DDEL RFF+R+RL F A AGL S E L +A Sbjct: 316 TAYLTVPKGWEELVGALETDDELRERFFARMRLFFFAAAGLSHSVRERLDRVAEAHCGER 375 Query: 367 MRIVISYGSTETAPVVCVSSGQSKRPDNIGVPIPGAEMKLAPSEDKFEIRLRGPMVTPGY 426 +R V G TETAP S+G P +GVP PG E+KLAP DK E R RGP V GY Sbjct: 376 IRTVAGLGMTETAPSCTFSTGAVDLPGYVGVPAPGCEVKLAPVGDKLEARFRGPHVMAGY 435 Query: 427 WRHPELAKDIFDEDGYYKMGDAGKLEDPSRPELGIVFDGRVAEDFKLVTGTWVRTTNVRI 486 WR PEL+ + FDE+GYY+ GDA +L DP RPELG++FDGR+AEDFKL +G +V +R Sbjct: 436 WREPELSAEAFDEEGYYRSGDALRLVDPERPELGLLFDGRIAEDFKLSSGVFVSVGPLRA 495 Query: 487 ACVSALAPLIQDAVVTGQDRDEVGLLAFPSLNGCRQLTKLP-GASLAELIDHPIIREAVA 545 + A AP IQD VVTG +R E+ LL FP L CR L LP A +AE++ P +R + Sbjct: 496 RVILAGAPYIQDVVVTGPNRPELALLIFPRLPECRALAGLPESAPVAEVLASPALRTWLR 555 Query: 546 QALARLNQGA-GSSGRIGRVLLMLTPPSIDTGEITDKGYINQRAVLRARANEVERLYSPT 604 + L LN+GA G++ R+ L+ PP ID GE+TDKG INQRAVL+ RA ++ LY+ Sbjct: 556 ELLDELNRGATGNASRLAWACLLAEPPCIDRGEVTDKGSINQRAVLQHRAALIDALYAGE 615 Query: 605 DAGVILPAA 613 DAG +LP A Sbjct: 616 DAGRVLPGA 624 >ref|YP_001242742.1| feruloyl-CoA synthase [Bradyrhizobium sp. BTAi1] gb|ABQ38836.1| trans-feruloyl-CoA synthase [Bradyrhizobium sp. BTAi1] Length = 617 Score = 492 bits (1266), Expect = e-136, Method: Compositional matrix adjust. Identities = 288/610 (47%), Positives = 372/610 (60%), Gaps = 22/610 (3%) Query: 12 FVSPPVIATP-ESGGIVLRSGLAPTEPFLSLH-EMLERAAELVPHNIFLQERSVFDRNLW 69 F P + A + G I+LRS P P E LE A VP FL ER + W Sbjct: 15 FAEPAIDAVRRDDGAIILRS-RTPLAPAARCSGEWLEHWARSVPDRTFLAERR---GDGW 70 Query: 70 NRVTYIEALQLVDQYGRALLNYGLTPDRPLAIISPNSINHALLALAAMSVGIPVAPISVA 129 +TY E V +L L PDRPLAI+S NSI HA+LALAA VG+PV+ IS A Sbjct: 71 TGLTYGETRAQVRAAAAWMLAQSLGPDRPLAILSDNSIAHAVLALAAQHVGVPVSAISPA 130 Query: 130 YSQMA-DLERVENIFRLLTPGMVFAETWAGFERALSAAAKAG-ANILVGNSTGVDQPVLT 187 YS M+ D E++ + RLL PG +F A F ALSA A I+ G+ G L Sbjct: 131 YSLMSTDHEKLRAMIRLLDPGAIFVAQRAPFAPALSAIESLHRATIIAGDGDGTSAVPLQ 190 Query: 188 LEKIRDPGQAVGSIRARFPVGPDTIAKVLFTSGSTGSSKGALVTQRMMCSNQEALTQCWP 247 PG V RA V PDT+AK+LFTSGSTG K + TQRMM ++Q+A Q W Sbjct: 191 SLIATTPGPEVD--RAFAAVTPDTVAKLLFTSGSTGQPKAVINTQRMMTTSQQAKAQLWT 248 Query: 248 FL-EAEP-PVAVDWLPWSHVFGGCLIFNCVLRHLGTMVIDDGRPVGSQFERTLQNIRELP 305 FL E E PV VDWLPWSH FG FN VL G++ ID G+P F+ +L N+R Sbjct: 249 FLREVEGGPVIVDWLPWSHTFGANHNFNLVLSQGGSLYIDGGKPAPGLFDTSLANLRSKV 308 Query: 306 PSIYLSVPKALDELVAALRKDDELCRRFFSRLRLIFSAGAGLPKSTWEALHELA----GR 361 P+IY +VP+ D LVAAL+ DD+L ++FFS +R IF A A LP++ W+AL+E++ GR Sbjct: 309 PTIYFNVPRGFDMLVAALKTDDDLRQQFFSGVRCIFYAAAALPQNLWDALNEMSLASVGR 368 Query: 362 WSPHEMRIVISYGSTETAPVVCVSSGQSKRPDNIGVPIPGAEMKLAPSEDKFEIRLRGPM 421 +P ++ ++G+TET+P+ ++R NIGVPIPG E+KL PS +K EIR+RGP Sbjct: 369 PAP----LLSAWGATETSPLATDCHFFAERAGNIGVPIPGTELKLVPSGEKLEIRVRGPN 424 Query: 422 VTPGYWRHPELAKDIFDEDGYYKMGDAGKLEDPSRPELGIVFDGRVAEDFKLVTGTWVRT 481 VTPGYW+ PEL FD++G+YK+GDA K DP PE G+VFDGR+ EDFKL +GTWV Sbjct: 425 VTPGYWKAPELTAAAFDDEGFYKIGDAVKFADPEHPERGLVFDGRITEDFKLSSGTWVNV 484 Query: 482 TNVRIACVSALAPLIQDAVVTGQDRDEVGLLAFPSLNGCRQLTKLPG-ASLAELIDHPII 540 +RIA ++ALAPL QD VV G DEV L P++ CR L AS+ E++ HP + Sbjct: 485 GALRIAGIAALAPLAQDIVVAGHGTDEVHFLVVPNIAACRSKAALANTASIGEVLAHPAV 544 Query: 541 REAVAQALARLN-QGAGSSGRIGRVLLMLTPPSIDTGEITDKGYINQRAVLRARANEVER 599 R+A+A+ + +L + GSS R LLM PPS+D GEITDKGYINQRAVL R++ V R Sbjct: 545 RDAIAEGMTKLRMEAVGSSRYATRALLMAEPPSLDAGEITDKGYINQRAVLSRRSDLVAR 604 Query: 600 LYSPTDAGVI 609 L T + +I Sbjct: 605 LQDDTASDLI 614