BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0128.p (354 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_03588599.1| gentisate 1,2-dioxygenase [Burkholderia multi... 459 e-127 gb|AAD49427.1|AF173167_1 gentisate 1,2-dioxygenase [Pseudomonas ... 409 e-112 dbj|BAC98955.1| gentisate 1,2-dioxygenase [Xanthobacter polyarom... 395 e-108 ref|YP_001202852.1| gentisate 1,2-dioxygenase [Bradyrhizobium sp... 392 e-107 ref|NP_766750.1| gentisate 1,2-dioxygenase [Bradyrhizobium japon... 388 e-106 >ref|ZP_03588599.1| gentisate 1,2-dioxygenase [Burkholderia multivorans CGD1] gb|EED96950.1| gentisate 1,2-dioxygenase [Burkholderia multivorans CGD1] Length = 354 Score = 459 bits (1180), Expect = e-127, Method: Compositional matrix adjust. Identities = 221/336 (65%), Positives = 259/336 (77%), Gaps = 1/336 (0%) Query: 15 KRNELYRRLDTVAATPLWNVMARLVTPQPQPLSAPGKWEYDRMRELLLEAGELITAKEAE 74 +R LY RLD V + PLW VM++LVTP+P+P P +W+Y MR+LLL+AGELITAKEAE Sbjct: 16 ERLALYDRLDKVDSAPLWAVMSQLVTPEPRPRCVPHRWDYAAMRKLLLDAGELITAKEAE 75 Query: 75 RRVLILENTGLRGMSQITQSLYAGLQMILPGEIAPSHRHAASALRFVLEGEGGYTAVDGE 134 RRVL+LEN G+RGMSQITQSLYAGLQ+ILPGEIAPSHRHAASALR V+EG GGYT+V GE Sbjct: 76 RRVLVLENPGIRGMSQITQSLYAGLQLILPGEIAPSHRHAASALRLVIEGTGGYTSVSGE 135 Query: 135 RIFMEVGDFIITPSWTFHDHGNHGKEPVIWLDGLDVPIVNLFATSFAQSYP-GEQQAISR 193 RI M GDFI+TPSWTFHDHGN G +PVIWLDGLDVPIVNLF SFA++YP G+ Q + R Sbjct: 136 RIPMAPGDFILTPSWTFHDHGNVGDDPVIWLDGLDVPIVNLFDASFAEAYPSGDVQKVER 195 Query: 194 PEGDARARYGSGLLPIEHVAGGSSSPVFHYPYKVAKASLDALLRAGTVDAWHGVKLQYVN 253 GDA ARYG LLP+E+V GSSSPVFHY Y K +L + G +DA HG KLQY N Sbjct: 196 TAGDAVARYGMNLLPVEYVPTGSSSPVFHYQYSRVKEALGQVALGGAIDACHGFKLQYAN 255 Query: 254 PLNGDYAMPTMAAYLQLLPRDFASLPYRSTDSMVFCVKDGQGESIVGEQVISWKRGDVFV 313 P G Y MPTM A++Q LP F YRSTD+ V+CV +GQG + + +VI W GDVFV Sbjct: 256 PTTGGYPMPTMGAFIQELPSRFNGRAYRSTDASVYCVLEGQGSTSINGEVIEWGEGDVFV 315 Query: 314 VPSWSTVTHRTEGEAVLFSFSDRAAQKKLGLWREEK 349 VPSWS +H T G++VLFSFSDR AQK LGLWRE + Sbjct: 316 VPSWSITSHCTTGKSVLFSFSDRPAQKALGLWREAR 351 >gb|AAD49427.1|AF173167_1 gentisate 1,2-dioxygenase [Pseudomonas alcaligenes] Length = 347 Score = 409 bits (1050), Expect = e-112, Method: Compositional matrix adjust. Identities = 185/340 (54%), Positives = 255/340 (75%) Query: 10 PETIAKRNELYRRLDTVAATPLWNVMARLVTPQPQPLSAPGKWEYDRMRELLLEAGELIT 69 P +R E Y +L++ PLW V+ +VT +P+ AP WE++ +E L+EAG LIT Sbjct: 7 PAVTKERKEFYSKLESHDLAPLWEVLNEVVTTKPKSNCAPHLWEFEVAKEFLMEAGTLIT 66 Query: 70 AKEAERRVLILENTGLRGMSQITQSLYAGLQMILPGEIAPSHRHAASALRFVLEGEGGYT 129 AKEAERRVLILEN GL+G+S+IT SLYAGLQ+ILPGE+AP+HRH+ SALRFV++G G T Sbjct: 67 AKEAERRVLILENPGLKGLSRITTSLYAGLQLILPGEVAPTHRHSQSALRFVVDGGGACT 126 Query: 130 AVDGERIFMEVGDFIITPSWTFHDHGNHGKEPVIWLDGLDVPIVNLFATSFAQSYPGEQQ 189 +VDGER M+VGDF+ITP W +HDH N +P+IW+DGLD+P+V LF TSFA+ Y + Q Sbjct: 127 SVDGERTTMQVGDFVITPPWAWHDHVNDSDKPMIWMDGLDLPMVTLFDTSFAEGYGEDIQ 186 Query: 190 AISRPEGDARARYGSGLLPIEHVAGGSSSPVFHYPYKVAKASLDALLRAGTVDAWHGVKL 249 I+RP GD+ ARYG+ +LP++ G SSP+F+YPY+ ++ +L+A+ +A D HG+K+ Sbjct: 187 EITRPNGDSLARYGANMLPVDFKQKGLSSPIFNYPYERSREALEAMKKANEWDPCHGLKM 246 Query: 250 QYVNPLNGDYAMPTMAAYLQLLPRDFASLPYRSTDSMVFCVKDGQGESIVGEQVISWKRG 309 QY+NPL+G AMPT+++++QLLP++F + YRSTD+ VF V +GQG++ +G++V WK Sbjct: 247 QYINPLDGMAAMPTISSFIQLLPKEFRTQTYRSTDATVFSVIEGQGKTRIGDKVFFWKAK 306 Query: 310 DVFVVPSWSTVTHRTEGEAVLFSFSDRAAQKKLGLWREEK 349 D FVVPSW V H +AVLFS+SDR AQ+KLG WRE + Sbjct: 307 DTFVVPSWYPVEHEASSDAVLFSYSDRVAQQKLGFWRESR 346 >dbj|BAC98955.1| gentisate 1,2-dioxygenase [Xanthobacter polyaromaticivorans] Length = 350 Score = 395 bits (1014), Expect = e-108, Method: Compositional matrix adjust. Identities = 181/340 (53%), Positives = 250/340 (73%) Query: 10 PETIAKRNELYRRLDTVAATPLWNVMARLVTPQPQPLSAPGKWEYDRMRELLLEAGELIT 69 P A+R Y+++D + T LW V+ ++TP+P+ P W + ++ LLEAG LIT Sbjct: 7 PMDTAERQNFYKKIDDQSYTALWTVLNNIITPEPRSNCLPHVWRFHEAKDYLLEAGGLIT 66 Query: 70 AKEAERRVLILENTGLRGMSQITQSLYAGLQMILPGEIAPSHRHAASALRFVLEGEGGYT 129 AKEAERRVL+LEN GLRG S+IT SLYAGLQ+++P E+AP+HRH+ SALR VL+G G +T Sbjct: 67 AKEAERRVLVLENPGLRGQSRITTSLYAGLQIVMPDEVAPAHRHSQSALRLVLDGNGAHT 126 Query: 130 AVDGERIFMEVGDFIITPSWTFHDHGNHGKEPVIWLDGLDVPIVNLFATSFAQSYPGEQQ 189 +VDGER M GDFIITP WT+HDHGN EP+IWLDGLD+ +V+LF SFA+ Y ++Q Sbjct: 127 SVDGERTIMSFGDFIITPPWTWHDHGNSTDEPMIWLDGLDIAVVSLFDASFAEEYGADEQ 186 Query: 190 AISRPEGDARARYGSGLLPIEHVAGGSSSPVFHYPYKVAKASLDALLRAGTVDAWHGVKL 249 I GD+ ARYG+ L+P++ V G +SP+F+YPY+ ++ +L+AL ++ D HG+K+ Sbjct: 187 PIVHNIGDSSARYGANLMPVDFVHKGLASPIFNYPYERSREALEALKKSQEWDRCHGLKM 246 Query: 250 QYVNPLNGDYAMPTMAAYLQLLPRDFASLPYRSTDSMVFCVKDGQGESIVGEQVISWKRG 309 ++VNP++G +AMPT+A LQLLP+ F++ PYRSTD+MVF V +G G+S +G + I W + Sbjct: 247 RFVNPVDGGWAMPTIATGLQLLPKGFSAAPYRSTDAMVFVVLEGSGKSQIGGKEIIWGKK 306 Query: 310 DVFVVPSWSTVTHRTEGEAVLFSFSDRAAQKKLGLWREEK 349 DVFV PSW VTH E V F++SDR AQ+KLGLWRE++ Sbjct: 307 DVFVAPSWHWVTHMPLEETVFFTYSDRVAQEKLGLWREDR 346 >ref|YP_001202852.1| gentisate 1,2-dioxygenase [Bradyrhizobium sp. ORS 278] emb|CAL74612.1| Putative gentisate 1,2-dioxygenase with a Cupin, RmlC-type domain [Bradyrhizobium sp. ORS 278] Length = 345 Score = 392 bits (1006), Expect = e-107, Method: Compositional matrix adjust. Identities = 179/335 (53%), Positives = 242/335 (72%) Query: 15 KRNELYRRLDTVAATPLWNVMARLVTPQPQPLSAPGKWEYDRMRELLLEAGELITAKEAE 74 +R Y+++D T LW VM+ L+TP+P+ P W++ +R+ ++EAG LITAKEAE Sbjct: 8 EREAFYKKIDRENMTALWTVMSDLITPEPKSGCRPHLWQFAAIRDYMIEAGRLITAKEAE 67 Query: 75 RRVLILENTGLRGMSQITQSLYAGLQMILPGEIAPSHRHAASALRFVLEGEGGYTAVDGE 134 RRVLILEN GLRG S+IT SLYAG+QM++PG+IAP+HRH+ SALRFVLEG+G +T VDGE Sbjct: 68 RRVLILENPGLRGQSKITTSLYAGIQMVIPGDIAPAHRHSQSALRFVLEGQGAFTTVDGE 127 Query: 135 RIFMEVGDFIITPSWTFHDHGNHGKEPVIWLDGLDVPIVNLFATSFAQSYPGEQQAISRP 194 R M+ GDFIITPS T+HDHGN EP+ WLDGLD+P+V SFAQ +QQ ++RP Sbjct: 128 RTLMQPGDFIITPSMTWHDHGNETAEPMFWLDGLDIPLVQFLDASFAQGSQEDQQKVARP 187 Query: 195 EGDARARYGSGLLPIEHVAGGSSSPVFHYPYKVAKASLDALLRAGTVDAWHGVKLQYVNP 254 GD+ ARYG LLP++ +SP+F+YPY + +L+ R+G DA HG+KL++ NP Sbjct: 188 AGDSFARYGHNLLPVDAKRKSRTSPIFNYPYAYTREALENAKRSGEWDACHGLKLKFSNP 247 Query: 255 LNGDYAMPTMAAYLQLLPRDFASLPYRSTDSMVFCVKDGQGESIVGEQVISWKRGDVFVV 314 GD+AMPT+ ++QLLP+ F + YRSTD+ VF +G+G S +G+ + W D+FVV Sbjct: 248 ETGDFAMPTIGTFIQLLPKGFETARYRSTDATVFTAIEGRGRSRIGDAIFEWGPRDLFVV 307 Query: 315 PSWSTVTHRTEGEAVLFSFSDRAAQKKLGLWREEK 349 PSW VTH + +AVLFSFSDR Q+KL L+RE++ Sbjct: 308 PSWQWVTHEADQDAVLFSFSDRPVQEKLDLFREDR 342 >ref|NP_766750.1| gentisate 1,2-dioxygenase [Bradyrhizobium japonicum USDA 110] dbj|BAC45375.1| gentisate 1,2-dioxygenase [Bradyrhizobium japonicum USDA 110] Length = 346 Score = 388 bits (997), Expect = e-106, Method: Compositional matrix adjust. Identities = 179/335 (53%), Positives = 241/335 (71%) Query: 15 KRNELYRRLDTVAATPLWNVMARLVTPQPQPLSAPGKWEYDRMRELLLEAGELITAKEAE 74 +R Y+++D T LW VM L+TP+P+ P W++D +R+ + EAG+LITAKEAE Sbjct: 9 EREAFYKKIDGENLTALWTVMGDLITPEPKSACRPHLWKFDVIRDYMREAGKLITAKEAE 68 Query: 75 RRVLILENTGLRGMSQITQSLYAGLQMILPGEIAPSHRHAASALRFVLEGEGGYTAVDGE 134 RRVL+LEN GLRG S+IT SLYAG+QM++PG++AP+HRH+ SALRFVLEG+G +TAVDGE Sbjct: 69 RRVLVLENPGLRGQSKITTSLYAGVQMVVPGDVAPAHRHSQSALRFVLEGKGAHTAVDGE 128 Query: 135 RIFMEVGDFIITPSWTFHDHGNHGKEPVIWLDGLDVPIVNLFATSFAQSYPGEQQAISRP 194 R ME GDFIITPS T+HDH N EP+ WLDGLD+P+V F SFA+ +QQ I++P Sbjct: 129 RTAMEPGDFIITPSMTWHDHSNETDEPMFWLDGLDIPLVQFFDCSFAEGSKEDQQRITKP 188 Query: 195 EGDARARYGSGLLPIEHVAGGSSSPVFHYPYKVAKASLDALLRAGTVDAWHGVKLQYVNP 254 GD+ ARYG LLP++ +SP+F YPY + +L+ + DA HG+KL++ NP Sbjct: 189 AGDSFARYGHNLLPVDVKRSSKTSPIFSYPYAYTREALEKARASQEWDACHGLKLKFSNP 248 Query: 255 LNGDYAMPTMAAYLQLLPRDFASLPYRSTDSMVFCVKDGQGESIVGEQVISWKRGDVFVV 314 GD+AMPT+ ++QLLP+ F + YRSTD+ VFC +G+G S +G+ V W D+FVV Sbjct: 249 ETGDFAMPTIGTFIQLLPKGFKTARYRSTDATVFCPIEGRGRSRIGDAVFEWGPRDLFVV 308 Query: 315 PSWSTVTHRTEGEAVLFSFSDRAAQKKLGLWREEK 349 PSW VTH + +AVLFSFSDR Q+KL L+RE++ Sbjct: 309 PSWHWVTHEADDDAVLFSFSDRPVQQKLDLFREDR 343