BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0136.p (472 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774948.1| major facilitator superfamily permease [Herb... 724 0.0 ref|YP_004751833.1| putative multidrug efflux protein [Collimona... 644 0.0 ref|YP_004751978.1| major facilitator superfamily (MFS) multidru... 577 e-162 ref|YP_001768073.1| major facilitator transporter [Methylobacter... 545 e-153 ref|YP_002496059.1| EmrB/QacA subfamily drug resistance transpor... 538 e-150 >ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] gb|ADJ63040.1| permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 446 Score = 724 bits (1869), Expect = 0.0, Method: Compositional matrix adjust. Identities = 357/444 (80%), Positives = 400/444 (90%) Query: 22 MDSTVISTSLPVLAIDMAVDPISLKLALTSYLVSLAVFIPISGWMADRFGALTIFRSAIC 81 MDSTVISTSLP LA+DM VDPISLKLALTSYLVSLAVFIPISGWMADRFG+LTIFRSAIC Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 82 VFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRSVDKANLVKALSYLTIPA 141 VFMLGSILCGVS +LP+FVAARF QGIGGAMMVPVGRLVILRSV+K NLVKALSYLTIPA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 142 MFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIANAKSESVAPLDWVGFVL 201 +FGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFI + K+E VAPLD GF+L Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 202 SALGCSMVMMGLATTGRHLVSGDISLACVVLGSLSLAAYVWHARRTEHPLIDLKLLRIET 261 SALGCS++MMGLAT GRHLVSG+++L CV+ G + LAAYVWHARR PLIDL LL+I T Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 262 LQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGLLTCASAAGAMFMKTIAAKFLK 321 L+AGV GGS +R+GIGAIPFLLPLMLQLGFG++ FHSG+LTCASAAGAMFMKTIAA+FL+ Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 300 Query: 322 RYGFRRVMIVNGACGVMSLAAIGFFTPATPYAVMLIVLLIGGCFRSLQFTSLNAISYADV 381 RYGFRRV++VNGA GV+ L AI FTP TPY +M+++LLIGGCFRSLQFTSLNAISYADV Sbjct: 301 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 360 Query: 382 DNNRLSQATSIASVAQQLSVGMGVVLGGFALQMSNHIQGHATLVSQDFWPAFLTVAVAAS 441 D RLSQATS+ASVAQQLS+GMGVVL GFALQMSN IQGHATLV+QDFWPAFL V A+ Sbjct: 361 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVAA 420 Query: 442 FSIPLLMRLPPDAGAELAGRNQAK 465 ++ +L +LP DAGAE+AGR+ + Sbjct: 421 CALLMLRQLPHDAGAEMAGRDDNR 444 >ref|YP_004751833.1| putative multidrug efflux protein [Collimonas fungivorans Ter331] gb|AEK61010.1| putative multidrug efflux protein [Collimonas fungivorans Ter331] Length = 464 Score = 644 bits (1661), Expect = 0.0, Method: Compositional matrix adjust. Identities = 315/462 (68%), Positives = 390/462 (84%) Query: 1 MALQRTFTIPLIVACALFMEQMDSTVISTSLPVLAIDMAVDPISLKLALTSYLVSLAVFI 60 MA+ R F PLIVACALFME MDSTVI+TSLP++A DM PI+LKLA+TSYLVSLAVFI Sbjct: 1 MAISRVFLTPLIVACALFMENMDSTVIATSLPMMARDMGESPIALKLAMTSYLVSLAVFI 60 Query: 61 PISGWMADRFGALTIFRSAICVFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLV 120 P+SGWMADRFGA T+FRSAI VFMLGSILCG+S +L +FVAARF QG+GGAMMVPVGRLV Sbjct: 61 PVSGWMADRFGARTVFRSAIGVFMLGSILCGMSDTLAQFVAARFLQGVGGAMMVPVGRLV 120 Query: 121 ILRSVDKANLVKALSYLTIPAMFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLAT 180 ILR+ KA LV+ALSYLTIPA+ GPV+GPPLGGFITTYFHWRWIFFIN+PISILG+YLAT Sbjct: 121 ILRTTTKAELVRALSYLTIPALLGPVIGPPLGGFITTYFHWRWIFFINVPISILGIYLAT 180 Query: 181 RFIANAKSESVAPLDWVGFVLSALGCSMVMMGLATTGRHLVSGDISLACVVLGSLSLAAY 240 R+I N +S+ PLDW+GF+LSA+GCS +M GLA+ GRHLV+GD+SL CV +G++SL AY Sbjct: 181 RYIENLRSDDTVPLDWIGFLLSAIGCSTLMFGLASAGRHLVAGDVSLICVAVGTVSLLAY 240 Query: 241 VWHARRTEHPLIDLKLLRIETLQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGL 300 VWHA RT HPLI+L+LL+I TL+ V GGSLFR+G+GA+PFLLPL+ QLGFG + F SGL Sbjct: 241 VWHAFRTPHPLINLRLLQIPTLRMNVFGGSLFRIGVGALPFLLPLLFQLGFGLTPFQSGL 300 Query: 301 LTCASAAGAMFMKTIAAKFLKRYGFRRVMIVNGACGVMSLAAIGFFTPATPYAVMLIVLL 360 LTCASAAGA+F+KT+ LK++GFR V+I N G +S+AA G +P+ ++++LL Sbjct: 301 LTCASAAGAIFVKTMTTTVLKKFGFRSVLIYNTLLGALSMAAFGVLKADSPHVFIILMLL 360 Query: 361 IGGCFRSLQFTSLNAISYADVDNNRLSQATSIASVAQQLSVGMGVVLGGFALQMSNHIQG 420 +GGC RS+QFTSLNAI+YA++D ++SQATS+ SVAQQL++G+GV +GGFALQ+SN +QG Sbjct: 361 LGGCVRSMQFTSLNAIAYAEIDQRQMSQATSLTSVAQQLAIGLGVTVGGFALQVSNQVQG 420 Query: 421 HATLVSQDFWPAFLTVAVAASFSIPLLMRLPPDAGAELAGRN 462 HAT+VS DFWPAFL +A+ A S+P ++L PDAGAEL+GR Sbjct: 421 HATIVSADFWPAFLLIALVALASLPFALKLQPDAGAELSGRR 462 >ref|YP_004751978.1| major facilitator superfamily (MFS) multidrug efflux pump [Collimonas fungivorans Ter331] gb|AEK61155.1| major facilitator superfamily (MFS) multidrug efflux pump [Collimonas fungivorans Ter331] Length = 470 Score = 577 bits (1487), Expect = e-162, Method: Compositional matrix adjust. Identities = 281/463 (60%), Positives = 364/463 (78%) Query: 2 ALQRTFTIPLIVACALFMEQMDSTVISTSLPVLAIDMAVDPISLKLALTSYLVSLAVFIP 61 A+ RT IPLIVACALFME MD+TVI+TSLPVLA D+ DP++LKLALTSY+V L VFIP Sbjct: 8 AVPRTLLIPLIVACALFMENMDATVIATSLPVLARDLGQDPLTLKLALTSYVVGLGVFIP 67 Query: 62 ISGWMADRFGALTIFRSAICVFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVI 121 ISGW+ADRFGA T+FR+AI VF+ GS++C S SL FVAARF QGIGGAMMVPVGR+VI Sbjct: 68 ISGWVADRFGARTVFRAAILVFLSGSLMCAASSSLAAFVAARFLQGIGGAMMVPVGRIVI 127 Query: 122 LRSVDKANLVKALSYLTIPAMFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATR 181 RSV + +LVKA+SYLTIP+ GPV+GPPLGGFITTYFHWRWIF IN+PISILG+YLA+R Sbjct: 128 FRSVPRTDLVKAISYLTIPSQLGPVIGPPLGGFITTYFHWRWIFLINVPISILGMYLASR 187 Query: 182 FIANAKSESVAPLDWVGFVLSALGCSMVMMGLATTGRHLVSGDISLACVVLGSLSLAAYV 241 +I N +S+ + PLD GFVLSA+G +++M+GL+ L++ + + A VLG L+L Y+ Sbjct: 188 YIENYRSDDLQPLDMKGFVLSAIGSTLLMLGLSLIDGELLAPEWAFAMCVLGGLTLYVYL 247 Query: 242 WHARRTEHPLIDLKLLRIETLQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGLL 301 HARR PL+DL+LLRI T +A V GGSLFR+G+GA+PFLLPL LQ+GFG +AFH+G + Sbjct: 248 LHARREPFPLLDLRLLRIPTFRASVIGGSLFRIGLGAVPFLLPLALQVGFGMNAFHAGTI 307 Query: 302 TCASAAGAMFMKTIAAKFLKRYGFRRVMIVNGACGVMSLAAIGFFTPATPYAVMLIVLLI 361 TCASA GA+FMK I L+RYGFR V+I N ++LA+ G FTP TPY VM+ V+L+ Sbjct: 308 TCASAFGAIFMKAIGTSVLRRYGFRSVLIWNAVLAGLALASYGLFTPTTPYLVMMAVVLL 367 Query: 362 GGCFRSLQFTSLNAISYADVDNNRLSQATSIASVAQQLSVGMGVVLGGFALQMSNHIQGH 421 GG F S+QFT LN ++YAD+D+ +S+ATS+ASV QQ+S+G+GV +GG A+ +S+ +QGH Sbjct: 368 GGFFPSMQFTCLNTMAYADLDSADVSRATSLASVVQQISLGLGVTIGGLAVHLSSRLQGH 427 Query: 422 ATLVSQDFWPAFLTVAVAASFSIPLLMRLPPDAGAELAGRNQA 464 +T+V+ DFWPAF+ + + + SIP+ LP +AG+ L G A Sbjct: 428 STIVAADFWPAFIVIGLFSLASIPVTRHLPLNAGSALTGHKPA 470 >ref|YP_001768073.1| major facilitator transporter [Methylobacterium sp. 4-46] gb|ACA15639.1| major facilitator superfamily MFS_1 [Methylobacterium sp. 4-46] Length = 471 Score = 545 bits (1405), Expect = e-153, Method: Compositional matrix adjust. Identities = 281/461 (60%), Positives = 345/461 (74%) Query: 9 IPLIVACALFMEQMDSTVISTSLPVLAIDMAVDPISLKLALTSYLVSLAVFIPISGWMAD 68 +PL+VA ALFME DSTVI+TSLP +A D+ DPI+LKLALTSYLVSLA+FIP+SGWMAD Sbjct: 7 VPLVVATALFMENTDSTVIATSLPAIAADLGEDPIALKLALTSYLVSLAIFIPVSGWMAD 66 Query: 69 RFGALTIFRSAICVFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRSVDKA 128 RFGA T+FR A+ VFM GS+ C + SL FV ARF QG+GGAMMVPVGRLV+LRSV KA Sbjct: 67 RFGARTVFRLALAVFMGGSLACAAADSLGWFVGARFLQGMGGAMMVPVGRLVLLRSVPKA 126 Query: 129 NLVKALSYLTIPAMFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIANAKS 188 LV+AL+ LTIPA+ GPV+GPPLGG ITT +WRWIFFIN+PI + G+ LATR+ A+ + Sbjct: 127 ELVQALATLTIPALVGPVIGPPLGGLITTTLNWRWIFFINLPIGLAGILLATRYFADLRE 186 Query: 189 ESVAPLDWVGFVLSALGCSMVMMGLATTGRHLVSGDISLACVVLGSLSLAAYVWHARRTE 248 PLD VGFVLS G + +M+GLA+ GRHL+ +S CV G LA Y HARRT Sbjct: 187 AERPPLDGVGFVLSGAGLASLMLGLASGGRHLLPASVSWGCVGAGLALLALYCGHARRTA 246 Query: 249 HPLIDLKLLRIETLQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGLLTCASAAG 308 HPLI L L+ T +A + GGSLFR+G GAIPFLLPLMLQLGFG HSGLLT A+AAG Sbjct: 247 HPLIRLDLMGYPTFRAAITGGSLFRIGTGAIPFLLPLMLQLGFGLDPLHSGLLTFAAAAG 306 Query: 309 AMFMKTIAAKFLKRYGFRRVMIVNGACGVMSLAAIGFFTPATPYAVMLIVLLIGGCFRSL 368 A+FMKT+AA+ L+ GFR V+ VN LAA G FTP+TP+A+++ VLLIGGCFRSL Sbjct: 307 ALFMKTLAARILRSLGFRTVLTVNAVLAACLLAANGLFTPSTPHALIVAVLLIGGCFRSL 366 Query: 369 QFTSLNAISYADVDNNRLSQATSIASVAQQLSVGMGVVLGGFALQMSNHIQGHATLVSQD 428 QFT++NAI+YADVD+ +S ATS+ASVAQQLS+ +GV G FAL+ + G A++ S+D Sbjct: 367 QFTAVNAIAYADVDSRDMSAATSLASVAQQLSLSIGVAFGAFALESAAQWHGRASVRSED 426 Query: 429 FWPAFLTVAVAASFSIPLLMRLPPDAGAELAGRNQAKAGSA 469 FWPAFL VA+ + S RL P AGAE++G + A A Sbjct: 427 FWPAFLAVALVSGLSAFAFARLAPTAGAEVSGHRRVTARPA 467 >ref|YP_002496059.1| EmrB/QacA subfamily drug resistance transporter [Methylobacterium nodulans ORS 2060] gb|ACL55756.1| drug resistance transporter, EmrB/QacA subfamily [Methylobacterium nodulans ORS 2060] Length = 471 Score = 538 bits (1385), Expect = e-150, Method: Compositional matrix adjust. Identities = 277/461 (60%), Positives = 339/461 (73%) Query: 9 IPLIVACALFMEQMDSTVISTSLPVLAIDMAVDPISLKLALTSYLVSLAVFIPISGWMAD 68 +PL+VA ALFME DSTVI+TSLP +A D+ DPI+LKLALT+YLVSLA+FIP+SGWMAD Sbjct: 7 VPLVVATALFMENTDSTVIATSLPAIAADLGEDPIALKLALTAYLVSLAIFIPVSGWMAD 66 Query: 69 RFGALTIFRSAICVFMLGSILCGVSQSLPEFVAARFFQGIGGAMMVPVGRLVILRSVDKA 128 RFGA T+FR A+ VFM GS+ C + SL FV ARF QG+GGAMMVPVGRLV+LRSV KA Sbjct: 67 RFGARTVFRWALAVFMAGSLACAAADSLGWFVGARFLQGMGGAMMVPVGRLVLLRSVPKA 126 Query: 129 NLVKALSYLTIPAMFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIANAKS 188 LV+AL+ LTIPA+ GPV+GPPLGGFITT HWRWIFFIN+PI G+ LAT + A+ + Sbjct: 127 ELVQALATLTIPALVGPVIGPPLGGFITTALHWRWIFFINLPIGFAGILLATHYFADLRE 186 Query: 189 ESVAPLDWVGFVLSALGCSMVMMGLATTGRHLVSGDISLACVVLGSLSLAAYVWHARRTE 248 PLD VGFVLS G + +M+GLA+ GRHL+ ++S C G L LA Y HARR Sbjct: 187 AERPPLDAVGFVLSGAGLASLMLGLASGGRHLLPPELSWGCTGAGLLLLALYWRHARRIA 246 Query: 249 HPLIDLKLLRIETLQAGVGGGSLFRLGIGAIPFLLPLMLQLGFGFSAFHSGLLTCASAAG 308 HPLI L LLR T +A + GGSLFR+G GAIPFLLPLMLQ+GFG SGLLT +AAG Sbjct: 247 HPLIRLDLLRYPTFRAAITGGSLFRIGTGAIPFLLPLMLQIGFGLDPLQSGLLTFVAAAG 306 Query: 309 AMFMKTIAAKFLKRYGFRRVMIVNGACGVMSLAAIGFFTPATPYAVMLIVLLIGGCFRSL 368 A+FMKT+AA+ L+ GFR V+ VN + L A G FTPATP+AV++ VLL+GGCFRSL Sbjct: 307 ALFMKTLAARILRALGFRTVLTVNAVIAAILLGANGLFTPATPHAVIVAVLLVGGCFRSL 366 Query: 369 QFTSLNAISYADVDNNRLSQATSIASVAQQLSVGMGVVLGGFALQMSNHIQGHATLVSQD 428 QFT++NAI+YADVD +S ATS+ASVAQQLS+ +GV G FAL+ + G + + D Sbjct: 367 QFTAVNAIAYADVDPRDMSAATSLASVAQQLSLSIGVAFGAFALESAAQWHGRTAVQAGD 426 Query: 429 FWPAFLTVAVAASFSIPLLMRLPPDAGAELAGRNQAKAGSA 469 FWPAFL VA+ + S RL P AGAE++G + A A Sbjct: 427 FWPAFLAVALVSGLSTLAFGRLAPTAGAEVSGHRRIAARPA 467