BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0124.p (176 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillu... 263 1e-68 ref|YP_004702118.1| enoyl-CoA hydratase-isomerase [Pseudomonas p... 204 5e-51 ref|YP_001668847.1| enoyl-CoA hydratase-isomerase [Pseudomonas p... 202 1e-50 emb|CAE45102.1| putative enoyl-CoA hydratase I [Pseudomonas sp. Y2] 202 2e-50 gb|AAC24329.2| PaaA [Pseudomonas putida] 201 3e-50 >ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] gb|ADJ65606.1| enoyl-CoA hydratase/isomerase protein [Herbaspirillum seropedicae SmR1] Length = 262 Score = 263 bits (671), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 135/188 (71%), Positives = 151/188 (80%), Gaps = 12/188 (6%) Query: 1 MAQTDAIGALLDVRPQYWKAIAGFSKPLLAAVNGYALGAGCEMLMHADIAIAGRGAKIGQ 60 M DAI LDVR QYW+AIA F KP+LAAVNGYALGAGCE+LMHADIAIA RGAKIGQ Sbjct: 75 MIHKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQ 134 Query: 61 PEINLGTIPGAGGTQRLIRTVGKPLAMKMVLSGEFLSADDALSAGLIAAAAGQG------ 114 PEIN+GT+PGAGGTQRLIRTVGKPLAMKMVLSGEF+SAD AL AGL+A Sbjct: 135 PEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERT 194 Query: 115 ---SHA---QVPLALRLAKEAMLKSYELGLEAGLNLERKSFSLLAASEDRREGIAAFKEK 168 +H+ + PLA+RLAKEAML+S+ELGLEAGL ERKSFSL+AAS DR+EGIAAF+EK Sbjct: 195 LALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEK 254 Query: 169 RTAQFVGR 176 R A F GR Sbjct: 255 RAAVFSGR 262 >ref|YP_004702118.1| enoyl-CoA hydratase-isomerase [Pseudomonas putida S16] gb|AEJ13238.1| enoyl-CoA hydratase-isomerase [Pseudomonas putida S16] Length = 264 Score = 204 bits (518), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 107/188 (56%), Positives = 135/188 (71%), Gaps = 12/188 (6%) Query: 1 MAQTDAIGALLDVRPQYWKAIAGFSKPLLAAVNGYALGAGCEMLMHADIAIAGRGAKIGQ 60 MA+ D +G L D R +W++IA F+KPL+AAVNGYALG GCE++M ADI IAG A+ GQ Sbjct: 77 MAERDLVGILNDPRVAHWQSIAAFAKPLIAAVNGYALGGGCELVMCADIVIAGSDARFGQ 136 Query: 61 PEINLGTIPGAGGTQRLIRTVGKPLAMKMVLSGEFLSADDALSAGLI------------A 108 PEINLG IPGAGGTQRL+R VGKPLAM+MVL+GE ++A AL AGL+ A Sbjct: 137 PEINLGIIPGAGGTQRLLRAVGKPLAMQMVLTGEAITAPHALQAGLVSEITQPELTVERA 196 Query: 109 AAAGQGSHAQVPLALRLAKEAMLKSYELGLEAGLNLERKSFSLLAASEDRREGIAAFKEK 168 + A+ PLA+RLAKEA+LK+ + L +GL ER +F+LLA + DR EGI AF+EK Sbjct: 197 LHIARSIAAKAPLAVRLAKEALLKAGDTDLASGLRFERHAFTLLAGTADRDEGIRAFQEK 256 Query: 169 RTAQFVGR 176 R A+F GR Sbjct: 257 RQARFQGR 264 >ref|YP_001668847.1| enoyl-CoA hydratase-isomerase [Pseudomonas putida GB-1] gb|ABY98511.1| Enoyl-CoA hydratase/isomerase [Pseudomonas putida GB-1] Length = 257 Score = 202 bits (514), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 106/188 (56%), Positives = 134/188 (71%), Gaps = 12/188 (6%) Query: 1 MAQTDAIGALLDVRPQYWKAIAGFSKPLLAAVNGYALGAGCEMLMHADIAIAGRGAKIGQ 60 MA+ D +G L D R +W++IA F+KPL+AAVNGYALG GCE++M ADI IAG A+ GQ Sbjct: 70 MAERDLVGILNDPRVAHWQSIAAFTKPLIAAVNGYALGGGCELVMCADIVIAGTDARFGQ 129 Query: 61 PEINLGTIPGAGGTQRLIRTVGKPLAMKMVLSGEFLSADDALSAGLI------------A 108 PEINLG IPGAGGTQRL+R VGKPLAM+MVL+GE ++A A AGL+ A Sbjct: 130 PEINLGIIPGAGGTQRLLRAVGKPLAMQMVLTGEAITARHAQQAGLVSEISQPELTVERA 189 Query: 109 AAAGQGSHAQVPLALRLAKEAMLKSYELGLEAGLNLERKSFSLLAASEDRREGIAAFKEK 168 + A+ PLA+RLAKEA+LK+ + L +GL ER +F+LLA + DR EGI AF+EK Sbjct: 190 MRVARSIAAKAPLAVRLAKEALLKAGDTDLASGLRFERHAFTLLAGTADRDEGIRAFQEK 249 Query: 169 RTAQFVGR 176 R A+F GR Sbjct: 250 RQARFQGR 257 >emb|CAE45102.1| putative enoyl-CoA hydratase I [Pseudomonas sp. Y2] Length = 256 Score = 202 bits (513), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 108/187 (57%), Positives = 130/187 (69%), Gaps = 12/187 (6%) Query: 1 MAQTDAIGALLDVRPQYWKAIAGFSKPLLAAVNGYALGAGCEMLMHADIAIAGRGAKIGQ 60 MA+ D +G L D R YW+ I F KPL+AAVNGYALG GCE+ MHADI IAG A+ GQ Sbjct: 69 MAERDLVGMLDDPRQGYWQRITQFPKPLIAAVNGYALGGGCELAMHADILIAGEDAQFGQ 128 Query: 61 PEINLGTIPGAGGTQRLIRTVGKPLAMKMVLSGEFLSADDALSAGLI------------A 108 PEINLG +PGAGGTQRL+R VGK LAM+MVL+GE +SA AL AGL+ A Sbjct: 129 PEINLGIMPGAGGTQRLLRAVGKSLAMQMVLAGESISARRALDAGLVSEITQPELTVERA 188 Query: 109 AAAGQGSHAQVPLALRLAKEAMLKSYELGLEAGLNLERKSFSLLAASEDRREGIAAFKEK 168 Q A+ PLA+RLAKEA+LK+ +L L +GL ER +F+LLA + DR EGI AF+EK Sbjct: 189 LQIAQRIAAKAPLAVRLAKEALLKAQDLDLASGLRFERHAFTLLAGTADRDEGIRAFQEK 248 Query: 169 RTAQFVG 175 R A F G Sbjct: 249 RQADFKG 255 >gb|AAC24329.2| PaaA [Pseudomonas putida] Length = 257 Score = 201 bits (511), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 106/188 (56%), Positives = 133/188 (70%), Gaps = 12/188 (6%) Query: 1 MAQTDAIGALLDVRPQYWKAIAGFSKPLLAAVNGYALGAGCEMLMHADIAIAGRGAKIGQ 60 MA+ D +G L D R +W+ IA F+KPL+AAVNGYALG GCE++M ADI IAG A+ GQ Sbjct: 70 MAERDLVGILNDPRVAHWQRIAAFAKPLIAAVNGYALGGGCELVMCADIVIAGSDARFGQ 129 Query: 61 PEINLGTIPGAGGTQRLIRTVGKPLAMKMVLSGEFLSADDALSAGLI------------A 108 PEINLG IPGAGGTQRL+R VGKPLAM+MVL+GE ++A A AGL+ A Sbjct: 130 PEINLGIIPGAGGTQRLLRAVGKPLAMQMVLTGEAITARHAQQAGLVSEITQPEFTVERA 189 Query: 109 AAAGQGSHAQVPLALRLAKEAMLKSYELGLEAGLNLERKSFSLLAASEDRREGIAAFKEK 168 + A+ PLA+RLAKEA+LK+ + L +GL ER +F+LLA + DR EGI AF+EK Sbjct: 190 MQIARNIAAKAPLAVRLAKEALLKAGDTDLASGLRFERHAFTLLAGTADRDEGIQAFQEK 249 Query: 169 RTAQFVGR 176 R A+F GR Sbjct: 250 RPARFQGR 257