BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0122.p (447 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777512.1| phenylacetyl-CoA-ligase [Herbaspirillum sero... 762 0.0 ref|YP_002008252.1| phenylacetyl-CoA ligase, phenylacetic acid d... 758 0.0 ref|YP_297943.1| phenylacetate-CoA ligase [Ralstonia eutropha JM... 749 0.0 ref|YP_004681511.1| phenylacetate-coenzyme A ligase PaaK [Cupria... 746 0.0 ref|YP_840876.1| phenylacetate-CoA ligase [Ralstonia eutropha H1... 742 0.0 >ref|YP_003777512.1| phenylacetyl-CoA-ligase [Herbaspirillum seropedicae SmR1] gb|ADJ65604.1| phenylacetyl-CoA-ligase protein [Herbaspirillum seropedicae SmR1] Length = 446 Score = 762 bits (1968), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/437 (83%), Positives = 402/437 (91%), Gaps = 2/437 (0%) Query: 1 MPQRTPQPGDLEAIETASKDELQALQLQRMKWSLKHAYDNVPHYRASFDAAGVHPDDLKT 60 M R PQPG+LE IETAS+DELQALQLQR+KWSL+HAYDNVPHYR +FD AGVHPDDLK+ Sbjct: 1 MTIRIPQPGELEPIETASRDELQALQLQRLKWSLRHAYDNVPHYRNAFDQAGVHPDDLKS 60 Query: 61 LADLAKFPFTGKKDLRDNYPFNMFAVPREQVVRIHASSGTTGKPTVVGYTQNDIDTWATV 120 L+DL++FPFT KKDLRD+YPF MFAVPREQVVR+HASSGTTGKPTVVGYTQNDIDTWATV Sbjct: 61 LSDLSRFPFTTKKDLRDHYPFGMFAVPREQVVRVHASSGTTGKPTVVGYTQNDIDTWATV 120 Query: 121 VARSIRAAGGRKGDIVHISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIQDF 180 VARSIRAAGGR+GDIV ISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLI+DF Sbjct: 121 VARSIRAAGGRRGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDF 180 Query: 181 KPSIIMVTPSYMLNIIEEFQRQGLDAVDSSLKVGIFGAEPWTDAMRAEIQARAGIDAVDI 240 KPSIIMVTPSYMLN+IEEFQRQG+D SSLKVGIFGAEPWTDAMR EIQ RAG+DAVDI Sbjct: 181 KPSIIMVTPSYMLNVIEEFQRQGMDPASSSLKVGIFGAEPWTDAMRREIQQRAGLDAVDI 240 Query: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIINPETGEVLPDGSDGELVFTSLTKEAL 300 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEII+P+TGEVLPDG +GELVFTSL+KEAL Sbjct: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIIDPDTGEVLPDGVEGELVFTSLSKEAL 300 Query: 301 PIIRYRTRDLTRLLPATSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKMPKLAPQ 360 P+IRYRTRDLTRLLP TSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILK+P+LAPQ Sbjct: 301 PMIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQ 360 Query: 361 YQLVVGRNGHLDTLGVTVELRPEVALSAGDIDALARELQHHIKTHVGVTTAVQVVASGKI 420 YQL + R+GH+D++ V ELR +V S ++AL +ELQH IKT+VG++T V VV +G I Sbjct: 361 YQLQIERHGHMDSMSVFAELREDV--SNEHVEALEKELQHSIKTYVGISTKVTVVPAGMI 418 Query: 421 ERSAVGKAKRVVDQRPK 437 ER++VGKAKRV+D R K Sbjct: 419 ERTSVGKAKRVIDNRKK 435 >ref|YP_002008252.1| phenylacetyl-CoA ligase, phenylacetic acid degradation [Cupriavidus taiwanensis LMG 19424] emb|CAQ72198.1| phenylacetyl-CoA ligase, phenylacetic acid degradation [Cupriavidus taiwanensis LMG 19424] Length = 449 Score = 758 bits (1957), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/449 (81%), Positives = 400/449 (89%), Gaps = 2/449 (0%) Query: 1 MPQRTPQPGDLEAIETASKDELQALQLQRMKWSLKHAYDNVPHYRASFDAAGVHPDDLKT 60 M QR P P +LE IE AS+DELQALQLQR+KWS++HAYDNVPHYR +F AGVHPDDLK+ Sbjct: 1 MVQRNPNPNELEPIERASRDELQALQLQRLKWSVRHAYDNVPHYRKAFQVAGVHPDDLKS 60 Query: 61 LADLAKFPFTGKKDLRDNYPFNMFAVPREQVVRIHASSGTTGKPTVVGYTQNDIDTWATV 120 L+DL+KFPF K+DLRDNYPF MFAVPREQV R+HASSGTTGKPTVVGYT DIDTWA+V Sbjct: 61 LSDLSKFPFLTKQDLRDNYPFGMFAVPREQVTRVHASSGTTGKPTVVGYTAKDIDTWASV 120 Query: 121 VARSIRAAGGRKGDIVHISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIQDF 180 VARSIRAAGGR GD+VH+SYGYGLFTGGLGAHYGAEK GCTVIPMSGGQTEKQVQLI++F Sbjct: 121 VARSIRAAGGRAGDLVHVSYGYGLFTGGLGAHYGAEKAGCTVIPMSGGQTEKQVQLIREF 180 Query: 181 KPSIIMVTPSYMLNIIEEFQRQGLDAVDSSLKVGIFGAEPWTDAMRAEIQARAGIDAVDI 240 +P IIMVTPSYMLN+IEE +RQG+D +SSLKVGIFGAEPWTDAMRAEI+ARAGIDAVDI Sbjct: 181 QPDIIMVTPSYMLNLIEEMERQGMDPSESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI 240 Query: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIINPETGEVLPDGSDGELVFTSLTKEAL 300 YGLSEVMGPGVA ECIESKDGPVIWEDHFY EII+P TGE+LPDGS+GELVFTSLTKEAL Sbjct: 241 YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEILPDGSEGELVFTSLTKEAL 300 Query: 301 PIIRYRTRDLTRLLPATSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKMPKLAPQ 360 P+IRYRTRDLTRLLP TSRSMRRIGKITGRSDDMLIIRGVNVFP+QIEELILK P LAPQ Sbjct: 301 PVIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPSQIEELILKAPALAPQ 360 Query: 361 YQLVVGRNGHLDTLGVTVELRPE--VALSAGDIDALARELQHHIKTHVGVTTAVQVVASG 418 YQLVV R+GHLD L V VE RPE +LSA D AL R+L+ IKT+VGVTT VQVVA+ Sbjct: 361 YQLVVTRDGHLDKLEVRVEARPERSASLSADDRAALERDLKDQIKTYVGVTTRVQVVAAE 420 Query: 419 KIERSAVGKAKRVVDQRPKVVHEAPAGAI 447 IER+ VGKA+RVVD RPKV HE P GAI Sbjct: 421 GIERTTVGKARRVVDMRPKVTHEQPVGAI 449 >ref|YP_297943.1| phenylacetate-CoA ligase [Ralstonia eutropha JMP134] gb|AAZ63099.1| phenylacetate-CoA ligase [Ralstonia eutropha JMP134] Length = 448 Score = 749 bits (1935), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/448 (80%), Positives = 400/448 (89%), Gaps = 1/448 (0%) Query: 1 MPQRTPQPGDLEAIETASKDELQALQLQRMKWSLKHAYDNVPHYRASFDAAGVHPDDLKT 60 M QRT P +LE IE AS+DELQALQL+R+KWS++HAYDNVPHYR SFDAAGVHPDDLK+ Sbjct: 1 MVQRTSHPDELEPIERASRDELQALQLERLKWSVRHAYDNVPHYRKSFDAAGVHPDDLKS 60 Query: 61 LADLAKFPFTGKKDLRDNYPFNMFAVPREQVVRIHASSGTTGKPTVVGYTQNDIDTWATV 120 L+DL+KFPF K+DLRDNYPF MFAVPRE+V R+HASSGTTGKPTVVGYT DIDTWA+V Sbjct: 61 LSDLSKFPFLAKQDLRDNYPFGMFAVPREKVARVHASSGTTGKPTVVGYTARDIDTWASV 120 Query: 121 VARSIRAAGGRKGDIVHISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIQDF 180 VARSIRAAGGR GD+VH+SYGYGLFTGGLGAHYGAEK GCTVIPMSGGQTEKQVQLI++F Sbjct: 121 VARSIRAAGGRPGDLVHVSYGYGLFTGGLGAHYGAEKAGCTVIPMSGGQTEKQVQLIREF 180 Query: 181 KPSIIMVTPSYMLNIIEEFQRQGLDAVDSSLKVGIFGAEPWTDAMRAEIQARAGIDAVDI 240 +P+IIMVTPSYMLN+IEE RQG+D +SSLKVGIFGAEPWTDAMRAEI+ARAGIDAVDI Sbjct: 181 QPNIIMVTPSYMLNLIEEMMRQGMDPAESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI 240 Query: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIINPETGEVLPDGSDGELVFTSLTKEAL 300 YGLSEVMGPGVA ECIESKDGPVIWEDHFY EII+P TGEVLPDG +GELVFTSLTKEAL Sbjct: 241 YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEVLPDGEEGELVFTSLTKEAL 300 Query: 301 PIIRYRTRDLTRLLPATSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKMPKLAPQ 360 P+IRYRTRDLTRLLP TSRSMRRIGKITGRSDDMLIIRGVNVFP+QIEELILK P LAPQ Sbjct: 301 PVIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPSQIEELILKAPALAPQ 360 Query: 361 YQLVVGRNGHLDTLGVTVELRPEVA-LSAGDIDALARELQHHIKTHVGVTTAVQVVASGK 419 YQLVV R+GHLD L V VE RP+ + LS D AL ++L+ IKT+VGVTT VQV+ + Sbjct: 361 YQLVVTRDGHLDKLEVRVEARPQSSGLSGNDRAALEQQLKEQIKTYVGVTTRVQVLDAEG 420 Query: 420 IERSAVGKAKRVVDQRPKVVHEAPAGAI 447 IER++VGKA+RV+D RPKV HE P GAI Sbjct: 421 IERTSVGKARRVLDMRPKVTHEQPVGAI 448 >ref|YP_004681511.1| phenylacetate-coenzyme A ligase PaaK [Cupriavidus necator N-1] gb|AEI80279.1| phenylacetate-coenzyme A ligase PaaK [Cupriavidus necator N-1] Length = 449 Score = 746 bits (1927), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/449 (80%), Positives = 397/449 (88%), Gaps = 2/449 (0%) Query: 1 MPQRTPQPGDLEAIETASKDELQALQLQRMKWSLKHAYDNVPHYRASFDAAGVHPDDLKT 60 M QR P P +LE IE AS+DELQALQL+R+KWS++HAYDNVPHYR +F AAGVHPDDL++ Sbjct: 1 MVQRIPNPNELEPIERASRDELQALQLERLKWSVRHAYDNVPHYRKAFHAAGVHPDDLQS 60 Query: 61 LADLAKFPFTGKKDLRDNYPFNMFAVPREQVVRIHASSGTTGKPTVVGYTQNDIDTWATV 120 L+DL+KFPF K+DLRDNYPF MFAVPRE+V R+HASSGTTGKPTVVGYT DIDTWA+V Sbjct: 61 LSDLSKFPFLTKQDLRDNYPFGMFAVPREKVARVHASSGTTGKPTVVGYTAKDIDTWASV 120 Query: 121 VARSIRAAGGRKGDIVHISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIQDF 180 VARSIRAAGGR GD+VH+SYGYGLFTGGLGAHYGAEK GCTVIPMSGGQTEKQVQLI++F Sbjct: 121 VARSIRAAGGRAGDLVHVSYGYGLFTGGLGAHYGAEKAGCTVIPMSGGQTEKQVQLIREF 180 Query: 181 KPSIIMVTPSYMLNIIEEFQRQGLDAVDSSLKVGIFGAEPWTDAMRAEIQARAGIDAVDI 240 +P+IIMVTPSYMLN+IEE +RQG+D +SSLKVGIFGAEPWTDAMRAEI+ARAGIDAVDI Sbjct: 181 QPNIIMVTPSYMLNLIEEMERQGMDPSESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI 240 Query: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIINPETGEVLPDGSDGELVFTSLTKEAL 300 YGLSEVMGPGVA ECIESKDGPVIWEDHFY EII+P TGEVLPDG +GELVFTSLTKEAL Sbjct: 241 YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEVLPDGEEGELVFTSLTKEAL 300 Query: 301 PIIRYRTRDLTRLLPATSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKMPKLAPQ 360 P+IRYRTRDLTRLLP TSRSMRRIGKITGRSDDMLIIRGVNVFP+QIEELILK P LAPQ Sbjct: 301 PVIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPSQIEELILKTPALAPQ 360 Query: 361 YQLVVGRNGHLDTLGVTVELR--PEVALSAGDIDALARELQHHIKTHVGVTTAVQVVASG 418 YQLVV R+GHLD L V VE R P AL A D L REL+ IKT+VGVTT VQVV + Sbjct: 361 YQLVVTRDGHLDKLEVRVEARPGPSAALCADDRATLERELKAQIKTYVGVTTRVQVVDAD 420 Query: 419 KIERSAVGKAKRVVDQRPKVVHEAPAGAI 447 IER+ VGKA+RV+D RPKV HE P GAI Sbjct: 421 GIERTTVGKARRVLDMRPKVTHEQPVGAI 449 >ref|YP_840876.1| phenylacetate-CoA ligase [Ralstonia eutropha H16] emb|CAJ96146.1| Phenylacetate-CoA ligase [Ralstonia eutropha H16] Length = 449 Score = 742 bits (1916), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/449 (79%), Positives = 395/449 (87%), Gaps = 2/449 (0%) Query: 1 MPQRTPQPGDLEAIETASKDELQALQLQRMKWSLKHAYDNVPHYRASFDAAGVHPDDLKT 60 M QR P P +LE IE AS+DELQALQL+R+KWS++HAYDNVPHYR +F AAGVHPDDL++ Sbjct: 1 MVQRIPNPNELEPIERASRDELQALQLERLKWSVRHAYDNVPHYRKAFHAAGVHPDDLQS 60 Query: 61 LADLAKFPFTGKKDLRDNYPFNMFAVPREQVVRIHASSGTTGKPTVVGYTQNDIDTWATV 120 L+DL+KFPF K+DLRDNYPF MFAVPRE+V R+HASSGTTGKPTVVGYT DIDTWA+V Sbjct: 61 LSDLSKFPFLTKQDLRDNYPFGMFAVPREKVARVHASSGTTGKPTVVGYTAKDIDTWASV 120 Query: 121 VARSIRAAGGRKGDIVHISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIQDF 180 VARSIRAAGGR GD+VH+SYGYGLFTGGLGAHYGAEK GCTVIPMSGGQTEKQVQLI++F Sbjct: 121 VARSIRAAGGRAGDLVHVSYGYGLFTGGLGAHYGAEKAGCTVIPMSGGQTEKQVQLIREF 180 Query: 181 KPSIIMVTPSYMLNIIEEFQRQGLDAVDSSLKVGIFGAEPWTDAMRAEIQARAGIDAVDI 240 +P+IIMVTPSYMLN+IEE +RQG+D +SSLKVGIFGAEPWTDAMRAEI+ARAGIDAVDI Sbjct: 181 EPNIIMVTPSYMLNLIEEMERQGMDPSESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI 240 Query: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIINPETGEVLPDGSDGELVFTSLTKEAL 300 YGLSEVMGPGVA ECIESKDGPVIWEDHFY EII+P TGEVLPDG +GELVFTSLTKEAL Sbjct: 241 YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEVLPDGEEGELVFTSLTKEAL 300 Query: 301 PIIRYRTRDLTRLLPATSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKMPKLAPQ 360 P+IRYRTRDLTRLLP TSRSMRRIGKITGRSDDMLIIRGVNVFP+QIEELILK P LAPQ Sbjct: 301 PVIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPSQIEELILKTPALAPQ 360 Query: 361 YQLVVGRNGHLDTLGVTVELRP--EVALSAGDIDALARELQHHIKTHVGVTTAVQVVASG 418 YQLVV R+GHLD L V VE RP L A D L REL+ IKT+VGVTT VQVV + Sbjct: 361 YQLVVTRDGHLDKLEVRVEARPGGSAPLCADDRAMLERELKDQIKTYVGVTTRVQVVDAD 420 Query: 419 KIERSAVGKAKRVVDQRPKVVHEAPAGAI 447 IER+ VGKA+RV+D RPK HE P GAI Sbjct: 421 GIERTTVGKARRVLDMRPKFTHEQPVGAI 449