BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0114.p (360 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoredu... 618 e-175 ref|YP_004681513.1| phenylacetic acid degradation NADH oxidoredu... 496 e-138 ref|YP_160277.1| hypothetical protein ebA5725 [Aromatoleum aroma... 491 e-137 ref|YP_297941.1| phenylacetate-CoA oxygenase/reductase subunit P... 486 e-135 ref|YP_840878.1| phenylacetic acid degradation protein E,flavodo... 484 e-134 >ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] gb|ADJ65599.1| phenylacetic acid degradation NADH oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 356 Score = 618 bits (1593), Expect = e-175, Method: Compositional matrix adjust. Identities = 294/360 (81%), Positives = 322/360 (89%), Gaps = 4/360 (1%) Query: 1 MSKFYPLTVGNVKNETRDTIVVSFDVPAELKQTFQYQQGQHLTLRSEINGEDLRRSYSIC 60 MSKFYPLT+ +VK ETRDTIVVSF VPAEL+ TF YQQGQHLTLRSEINGEDLRRSYSIC Sbjct: 1 MSKFYPLTISDVKQETRDTIVVSFAVPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLDAANQKHYLAFAA 120 SAVQ+ QLRVAIKR GG+FS WAN++ PGQ + VMPPMGHFN+PL+A N+KHYLAFAA Sbjct: 61 SAVQERQLRVAIKRAPGGLFSNWANESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAA 120 Query: 121 GSGITPMLSIIKTTLLAEPGSRFTLVYGNRASSSVSSVIFKEELTDLKDIYLERLNVVYV 180 GSGITPM+SIIKTTLL+EP S FTLVY NRAS SSVIFKEELTDLKD YLER NVVYV Sbjct: 121 GSGITPMMSIIKTTLLSEPHSHFTLVYANRAS---SSVIFKEELTDLKDAYLERFNVVYV 177 Query: 181 MSREQQDVELFNGRITREKCDQFFKSWIDLDDIDAAFICGPEEMLNAVSASLQANGLDKS 240 MSREQQDVELFNGRI R KCD FF SWIDL D+DAAF+CGPEEM+ AV+ASLQA+GL K+ Sbjct: 178 MSREQQDVELFNGRIDRAKCDAFFASWIDLKDVDAAFLCGPEEMVQAVAASLQAHGLPKT 237 Query: 241 AIKVELFAASIPKHKAVAARPVVGKQECEVTVIVDGYHTVFTMEKEKESLLDAGLKNGID 300 IK ELFAAS P +A AAR VVGKQECEVTVIVDGYH VFTM+KEKES+L+AGLK+GID Sbjct: 238 QIKTELFAASTPP-RAHAARTVVGKQECEVTVIVDGYHNVFTMDKEKESVLEAGLKHGID 296 Query: 301 MRYSCKGGVCATCRCKIVEGKVDMDANYCLEDYEIARGFVLSCQSFPVTDKLLIDFDQDN 360 +RYSCKGGVCATCRCK+VEGKVDMDANY LEDYEIARGFVLSCQSFPV+DKLL+DFDQDN Sbjct: 297 LRYSCKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQDN 356 >ref|YP_004681513.1| phenylacetic acid degradation NADH oxidoreductase PaaE [Cupriavidus necator N-1] gb|AEI80281.1| phenylacetic acid degradation NADH oxidoreductase PaaE [Cupriavidus necator N-1] Length = 358 Score = 496 bits (1276), Expect = e-138, Method: Compositional matrix adjust. Identities = 236/361 (65%), Positives = 283/361 (78%), Gaps = 4/361 (1%) Query: 1 MSKFYPLTVGNVKNETRDTIVVSFDVPAELKQTFQYQQGQHLTLRSEINGEDLRRSYSIC 60 MSKF+ LTV +V ETRD + V+F VP EL T++Y QGQHLTLR+ I GED+RRSYSIC Sbjct: 1 MSKFHELTVASVTRETRDAVAVTFAVPDELADTYRYIQGQHLTLRTGIGGEDVRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLDAANQKHYLAFAA 120 SAVQD QLRVAIKR GG+FS WAN+ L+PG L+VMPP GHF++PL AA +HY+AFAA Sbjct: 61 SAVQDAQLRVAIKRVDGGLFSNWANEQLQPGMKLEVMPPSGHFHVPLSAAQARHYVAFAA 120 Query: 121 GSGITPMLSIIKTTLLAEPGSRFTLVYGNRASSSVSSVIFKEELTDLKDIYLERLNVVYV 180 GSGITPMLSIIKTTL AEP SRFTL YGNRAS SSV+FKEEL DLKD YL+R N+V+V Sbjct: 121 GSGITPMLSIIKTTLQAEPESRFTLFYGNRAS---SSVLFKEELEDLKDTYLQRFNLVFV 177 Query: 181 MSREQQDVELFNGRITREKCDQFFKSWIDLDDIDAAFICGPEEMLNAVSASLQANGLDKS 240 +SREQ D++LFNGRI EK + K W+ DID AFICGP M+ VS +L NG+DK+ Sbjct: 178 LSREQLDIDLFNGRIDGEKVNALLKHWVKPQDIDIAFICGPHSMMEEVSQALLDNGVDKA 237 Query: 241 AIKVELFAASIPKHKAVA-ARPVVGKQECEVTVIVDGYHTVFTMEKEKESLLDAGLKNGI 299 IK ELFA SIP + A A VG+Q+CEVTVI DG FT+EK KE++LDA L GI Sbjct: 238 RIKRELFATSIPSARPAAHAHKQVGQQQCEVTVIQDGRTRSFTLEKNKETVLDAALAQGI 297 Query: 300 DMRYSCKGGVCATCRCKIVEGKVDMDANYCLEDYEIARGFVLSCQSFPVTDKLLIDFDQD 359 ++ YSCKGGVC+TCRCK +EG+VDMD N+ LEDYE+ARGF+LSCQS+ V+DKL+IDFDQ+ Sbjct: 298 ELPYSCKGGVCSTCRCKRIEGEVDMDVNFALEDYEVARGFILSCQSYAVSDKLVIDFDQE 357 Query: 360 N 360 Sbjct: 358 T 358 >ref|YP_160277.1| hypothetical protein ebA5725 [Aromatoleum aromaticum EbN1] emb|CAI09376.1| similar to aerobic phenylacetate degradation protein [Aromatoleum aromaticum EbN1] Length = 358 Score = 491 bits (1264), Expect = e-137, Method: Compositional matrix adjust. Identities = 233/361 (64%), Positives = 284/361 (78%), Gaps = 4/361 (1%) Query: 1 MSKFYPLTVGNVKNETRDTIVVSFDVPAELKQTFQYQQGQHLTLRSEINGEDLRRSYSIC 60 MSKF+ + V +V+ ETRD IVV+FDVP L +F Y GQHLTLR+EI GED+RRSYSIC Sbjct: 1 MSKFHDMRVASVRPETRDAIVVTFDVPPSLADSFHYAPGQHLTLRTEIGGEDVRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLDAANQKHYLAFAA 120 S + LR+AIKR GG+FS WAND +KPG S++VMPP GHF + D + ++Y AFAA Sbjct: 61 SGADERALRIAIKRVHGGLFSNWANDFMKPGVSVEVMPPSGHFGVAPDPQHHRNYAAFAA 120 Query: 121 GSGITPMLSIIKTTLLAEPGSRFTLVYGNRASSSVSSVIFKEELTDLKDIYLERLNVVYV 180 GSGITP+LS IKT L AE GSRFTLVYGNR+S SSV+F+EEL DLKD Y ERLN+V+V Sbjct: 121 GSGITPILSQIKTILAAEQGSRFTLVYGNRSS---SSVMFREELADLKDQYGERLNLVHV 177 Query: 181 MSREQQDVELFNGRITREKCDQFFKSWIDLDDIDAAFICGPEEMLNAVSASLQANGLDKS 240 +SREQQD+ELFNGRI RE+CD WID ID AFICGP +M+ AVSASLQA+G+ KS Sbjct: 178 LSREQQDIELFNGRIDRERCDALLTRWIDAKRIDVAFICGPMDMMEAVSASLQAHGVAKS 237 Query: 241 AIKVELFAASIPK-HKAVAARPVVGKQECEVTVIVDGYHTVFTMEKEKESLLDAGLKNGI 299 AIK+ELFA S+PK + V + PV G ++CEVTVI DG FTM K ++S+LDAGL GI Sbjct: 238 AIKMELFATSLPKGPRPVHSHPVKGTEDCEVTVIQDGRVRQFTMPKNRDSVLDAGLGQGI 297 Query: 300 DMRYSCKGGVCATCRCKIVEGKVDMDANYCLEDYEIARGFVLSCQSFPVTDKLLIDFDQD 359 +M YSCKGGVC+TCRCK+++G+VD D N+ LEDYEIARGF LSCQS+PVTDKL++DFDQ+ Sbjct: 298 EMPYSCKGGVCSTCRCKVIDGEVDHDTNFALEDYEIARGFRLSCQSYPVTDKLVLDFDQE 357 Query: 360 N 360 Sbjct: 358 T 358 >ref|YP_297941.1| phenylacetate-CoA oxygenase/reductase subunit PaaK [Ralstonia eutropha JMP134] gb|AAZ63097.1| Phenylacetate-CoA oxygenase/reductase, PaaK subunit [Ralstonia eutropha JMP134] Length = 358 Score = 486 bits (1251), Expect = e-135, Method: Compositional matrix adjust. Identities = 232/361 (64%), Positives = 281/361 (77%), Gaps = 4/361 (1%) Query: 1 MSKFYPLTVGNVKNETRDTIVVSFDVPAELKQTFQYQQGQHLTLRSEINGEDLRRSYSIC 60 MSKF+ LTV +V ETRD + V+F VP +L T++Y QGQHLTLR+ I GED+RRSYSIC Sbjct: 1 MSKFHELTVASVTRETRDAVAVTFAVPEDLADTYRYVQGQHLTLRAGIGGEDVRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLDAANQKHYLAFAA 120 SAVQD +LRVAIKR GG+FS WAND L+PG +L+VMPP GHF++PL + KHY+AFAA Sbjct: 61 SAVQDRRLRVAIKRVDGGMFSNWANDALQPGMTLEVMPPSGHFHVPLSDTHSKHYVAFAA 120 Query: 121 GSGITPMLSIIKTTLLAEPGSRFTLVYGNRASSSVSSVIFKEELTDLKDIYLERLNVVYV 180 GSGITPMLSIIKTTL+ EP SRFTL YGNRAS SSV+FKEEL DLKD YLER N+V++ Sbjct: 121 GSGITPMLSIIKTTLMTEPDSRFTLFYGNRAS---SSVLFKEELEDLKDTYLERFNLVFI 177 Query: 181 MSREQQDVELFNGRITREKCDQFFKSWIDLDDIDAAFICGPEEMLNAVSASLQANGLDKS 240 +SREQ D++LFNGRI EK + W+ DID AFICGP M+ VS +LQ NG+DK+ Sbjct: 178 LSREQLDIDLFNGRIDGEKVRALLRHWVRPQDIDVAFICGPHSMMEEVSQALQENGVDKA 237 Query: 241 AIKVELFAASI-PKHKAVAARPVVGKQECEVTVIVDGYHTVFTMEKEKESLLDAGLKNGI 299 IK ELFA SI A A +G+Q+CEVTVI DG FT+EK KE++LDA L GI Sbjct: 238 RIKRELFATSIPAARPAAHAHKQLGQQQCEVTVIQDGRTRQFTLEKNKETILDAALAQGI 297 Query: 300 DMRYSCKGGVCATCRCKIVEGKVDMDANYCLEDYEIARGFVLSCQSFPVTDKLLIDFDQD 359 ++ YSCKGGVC+TCRCK +EG+VDMD N+ LEDYE+ARGF+LSCQS+ VTDKL+IDFDQ+ Sbjct: 298 ELPYSCKGGVCSTCRCKRIEGEVDMDVNFALEDYEVARGFILSCQSYAVTDKLVIDFDQE 357 Query: 360 N 360 Sbjct: 358 T 358 >ref|YP_840878.1| phenylacetic acid degradation protein E,flavodoxin reductase [Ralstonia eutropha H16] emb|CAJ96148.1| phenylacetic acid degradation protein E,flavodoxin reductase [Ralstonia eutropha H16] Length = 358 Score = 484 bits (1245), Expect = e-134, Method: Compositional matrix adjust. Identities = 233/361 (64%), Positives = 280/361 (77%), Gaps = 4/361 (1%) Query: 1 MSKFYPLTVGNVKNETRDTIVVSFDVPAELKQTFQYQQGQHLTLRSEINGEDLRRSYSIC 60 MSKF+ LTV +V ETRD + V+F VP EL T++Y QGQHLTLR+ I GED+RRSYSIC Sbjct: 1 MSKFHELTVASVTRETRDAVAVTFAVPDELADTYRYIQGQHLTLRTGIGGEDVRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNLPLDAANQKHYLAFAA 120 SAVQD QLRVAIKR GG+FS WAN+ L+PG L+VMPP GHF++PL A +HY+AFAA Sbjct: 61 SAVQDAQLRVAIKRVDGGLFSNWANEQLQPGMKLEVMPPSGHFHVPLSATQARHYVAFAA 120 Query: 121 GSGITPMLSIIKTTLLAEPGSRFTLVYGNRASSSVSSVIFKEELTDLKDIYLERLNVVYV 180 GSGITPMLSIIKTTL AEP SRFTL YGNRAS SSV+FKEEL DLKD YL+R N+V+V Sbjct: 121 GSGITPMLSIIKTTLEAEPQSRFTLFYGNRAS---SSVLFKEELEDLKDTYLQRFNLVFV 177 Query: 181 MSREQQDVELFNGRITREKCDQFFKSWIDLDDIDAAFICGPEEMLNAVSASLQANGLDKS 240 +SREQ D++LFNGRI EK + K W+ DID AFICGP M+ VS +L NG+DK+ Sbjct: 178 LSREQLDIDLFNGRIDGEKVNALLKHWVKPQDIDIAFICGPHSMMEEVSQALLDNGVDKA 237 Query: 241 AIKVELFAASI-PKHKAVAARPVVGKQECEVTVIVDGYHTVFTMEKEKESLLDAGLKNGI 299 IK ELFA SI A A VG+Q+CEVTVI DG FT+EK KE++LDA L GI Sbjct: 238 RIKRELFATSIPAARPAAHAHKQVGQQQCEVTVIQDGRTRSFTLEKNKETVLDAALAQGI 297 Query: 300 DMRYSCKGGVCATCRCKIVEGKVDMDANYCLEDYEIARGFVLSCQSFPVTDKLLIDFDQD 359 ++ YSCKGGVC+TCRCK +EG+VDMD N+ LEDYE+ARGF+LSCQS+ V+D+L+IDFDQ+ Sbjct: 298 ELPYSCKGGVCSTCRCKRIEGEVDMDVNFALEDYEVARGFILSCQSYAVSDRLVIDFDQE 357 Query: 360 N 360 Sbjct: 358 T 358