BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0070.p (382 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_002545803.1| salicylate hydroxylase protein [Agrobacteriu... 300 2e-79 ref|YP_004751335.1| monooxygenase [Collimonas fungivorans Ter331... 291 1e-76 ref|NP_822056.1| monooxygenase [Streptomyces avermitilis MA-4680... 284 2e-74 ref|YP_004812554.1| FAD-binding monooxygenase [Streptomyces viol... 283 3e-74 ref|NP_624801.1| monooxygenase [Streptomyces coelicolor A3(2)] >... 283 5e-74 >ref|YP_002545803.1| salicylate hydroxylase protein [Agrobacterium radiobacter K84] gb|ACM27872.1| salicylate hydroxylase protein [Agrobacterium radiobacter K84] Length = 385 Score = 300 bits (769), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 171/380 (45%), Positives = 229/380 (60%), Gaps = 8/380 (2%) Query: 1 MRQQPSIAIIGAGPAGLTAANVFHRHGWQCEVFESDASAASRDQGGSLDLHPEDGQLALK 60 M P IAIIGAGPAGLT A + H+ G + VFE + A +R QGG+LDLH E GQLAL+ Sbjct: 1 MTSYPRIAIIGAGPAGLTLARILHQGGVKTTVFEREEHALARPQGGTLDLHVESGQLALR 60 Query: 61 KAGLFAQFMAVARHEDQGLRIIDGASGAVMREFAPAEG-EGDRPEIDRDVLRQLLLEPLG 119 AGL+ +F+ +AR+EDQG R+ D +G ++ FA + E DRPE+DR LRQ+LL L Sbjct: 61 HAGLYEEFLEIARYEDQGSRLYD-KNGTLL--FADDDTDESDRPEVDRTALRQILLASLP 117 Query: 120 GNVVHWAAQIRALTVLPDGRVTLQLESGSVGPFDLVIGADGAWSKVRTVLTDIFPAYTGV 179 +++ W + + DG L E G+ GPFDLVIGADG+WSKVR +++ P Y+G+ Sbjct: 118 ADIIRWNHGLLEVYPREDGAYDLVFEHGTAGPFDLVIGADGSWSKVRPLVSHYHPQYSGI 177 Query: 180 TFVELWFDDVDQAHPAAAELVGHGTMFALRDQAGIIGQRNGKGRLRVYAAFRTRAEDGDR 239 +F+E DDVD +HP A LVG G + D G+I QRNG +R YA FR A+ ++ Sbjct: 178 SFIEFGIDDVDASHPELAALVGRGKIGVEADGRGMIVQRNGNAHIRGYAIFRVPADWANK 237 Query: 240 P-DKTPANLGKDELLARFPGWAPSLTSLLRDA-DHIAAIRPIVTLPAQCRWQHQSGLTLV 297 D + +L A F GWAP + +L+ + DHI + RPI LP RWQ++ G+TL+ Sbjct: 238 AFDFASPASARAQLAAEFEGWAPKILALIHASNDHIVS-RPIYALPVGHRWQNRIGITLL 296 Query: 298 GDAAHVMPPL-GTGVNLAMLDAAELAENLIHAADWRLAVQASERVMLDRSSAIASQCIAS 356 GDAAHVM P G GVN AMLDAAEL L+ DWR AV+A E M +R A + Sbjct: 297 GDAAHVMSPFGGEGVNAAMLDAAELGRQLLENDDWRNAVRAYEAEMFERVMEPAEHAAEA 356 Query: 357 FDAMFGPDAHQSGVGHFDAH 376 + + H+ AH Sbjct: 357 VATELSHLGAELSLQHYKAH 376 >ref|YP_004751335.1| monooxygenase [Collimonas fungivorans Ter331] gb|AEK60512.1| monooxygenase [Collimonas fungivorans Ter331] Length = 370 Score = 291 bits (746), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 161/347 (46%), Positives = 211/347 (60%), Gaps = 5/347 (1%) Query: 1 MRQQPSIAIIGAGPAGLTAANVFHRHGWQCEVFESDASAASRDQGGSLDLHPEDGQLALK 60 M + IAI+GAGP GLT A + H+HG VFE ++ + R QGG+LDLH G AL+ Sbjct: 1 MNTKLRIAIVGAGPGGLTLARILHQHGIAATVFEQESHSLVRPQGGTLDLHEGSGLQALR 60 Query: 61 KAGLFAQFMAVARHEDQGLRIIDGASGAVMREFAPAEGEGDRPEIDRDVLRQLLLEPLGG 120 +A L +F+A+AR+EDQG R++D + F + GDRPE+DR LR +LL+ L Sbjct: 61 QADLMDEFLAIARYEDQGSRLLDKVGNVL---FEDQDTSGDRPEVDRTELRAMLLQALPA 117 Query: 121 NVVHWAAQIRALTVLPDGRVTLQLESGSVGPFDLVIGADGAWSKVRTVLTDIFPAYTGVT 180 + V W +R + DGR L + GS GPFDLV+GADGAWS++R +L+ P Y+G+T Sbjct: 118 SCVRWGCSMREVQARDDGRWDLVFDHGSEGPFDLVVGADGAWSRIRPLLSPYRPQYSGLT 177 Query: 181 FVELWFDDVDQAHPAAAELVGHGTMFALRDQAGIIGQRNGKGRLRVYAAFRTRAE-DGDR 239 F+E DDVD++HPA ++LVG G M D II QRNG +R YA FR A+ R Sbjct: 178 FIECGIDDVDRSHPALSQLVGRGKMGVEADCKEIIAQRNGNAHIRAYAIFRVPADWAARR 237 Query: 240 PDKTPANLGKDELLARFPGWAPSLTSLLRDADHIAAIRPIVTLPAQCRWQHQSGLTLVGD 299 D T + EL+ F G+AP + L R ++ AIRPI LP W ++ GLTL+GD Sbjct: 238 FDLTSPVSMRAELIKEFDGFAPEILDLFRASNDNFAIRPIFALPVGHCWANRHGLTLLGD 297 Query: 300 AAHVMPPL-GTGVNLAMLDAAELAENLIHAADWRLAVQASERVMLDR 345 AAHVM P G GVN AM DAAELA LI +DW AV E +M R Sbjct: 298 AAHVMSPFDGDGVNNAMFDAAELARLLIERSDWAEAVAEYEALMFAR 344 >ref|NP_822056.1| monooxygenase [Streptomyces avermitilis MA-4680] dbj|BAC68591.1| putative monooxygenase [Streptomyces avermitilis MA-4680] Length = 374 Score = 284 bits (726), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 159/351 (45%), Positives = 213/351 (60%), Gaps = 7/351 (1%) Query: 7 IAIIGAGPAGLTAANVFHRHGWQCEVFESDASAASRDQGGSLDLHPEDGQLALKKAGLFA 66 I+IIGAGP GLT A + +HG V++ D A SR+QGGSLDLH EDGQLAL++AGL Sbjct: 11 ISIIGAGPGGLTCARILQQHGIPVTVYDRDPEANSRNQGGSLDLHEEDGQLALREAGLLE 70 Query: 67 QFMAVARHEDQGLRIIDGASGAVMREFAPAEGEGDRPEIDRDVLRQLLLEPLGGNVVHWA 126 +F A+AR E Q +R +D A G V+ P EGE PEIDR LR LLL L V Sbjct: 71 EFFALARLEGQEMRQMDPA-GRVLDHHLPDEGETVSPEIDRGQLRDLLLRSLVAGTVQRG 129 Query: 127 AQIRALTVLPDGRVTLQLESGSVGPFDLVIGADGAWSKVRTVLTDIFPAYTGVTFVELWF 186 + +++ DG TL G+ DLVIGADGA+S+VRT ++ P YTGV+F+E WF Sbjct: 130 RTLESVSGPADGPRTLTFTDGTAVEADLVIGADGAFSRVRTAVSSATPRYTGVSFLEAWF 189 Query: 187 DDVDQAHPAAAELVGHGTMFALRDQAGIIGQRNGKGRLRVYAAFRTR----AEDGDRPDK 242 DD++ AHP ++LVG G+ + G+ QRN G +RVY R A +G RP+ Sbjct: 190 DDMEHAHPELSQLVGKGSAHIADGERGLFAQRNSGGHMRVYIMQRVPVDWIAANGLRPED 249 Query: 243 TPANLGKDELLARFPGWAPSLTSLLRDADHIAAIRPIVTLPAQCRWQHQSGLTLVGDAAH 302 T G LL+ + W+P + ++ D D RP+ LP W+H + L+GDAAH Sbjct: 250 TEGIRGH--LLSEYAAWSPQILRMITDNDGAYVDRPLFALPVPHIWEHSPSVALLGDAAH 307 Query: 303 VMPPLGTGVNLAMLDAAELAENLIHAADWRLAVQASERVMLDRSSAIASQC 353 +MPPLG GVNLAMLDA+ELA L+H++ AV + E+ ML RS+ +A + Sbjct: 308 LMPPLGVGVNLAMLDASELALALVHSSTIDDAVHSYEKSMLPRSADMAQKL 358 >ref|YP_004812554.1| FAD-binding monooxygenase [Streptomyces violaceusniger Tu 4113] gb|AEM82274.1| FAD-binding monooxygenase [Streptomyces violaceusniger Tu 4113] Length = 392 Score = 283 bits (725), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 177/382 (46%), Positives = 216/382 (56%), Gaps = 7/382 (1%) Query: 1 MRQQPSIAIIGAGPAGLTAANVFHRHGWQCEVFESDASAASRDQGGSLDLHPEDGQLALK 60 M P IAI+G G GLT A V H HG V+E DASA +RDQGGSLDL PE GQ AL Sbjct: 1 MSGAPRIAIVGGGLGGLTLARVLHVHGIASTVYELDASATARDQGGSLDLDPESGQRALL 60 Query: 61 KAGLFAQFMAVARHEDQGLRIIDGASGAVMREFAPAEGEGD----RPEIDRDVLRQLLLE 116 AGL +F ++R ED LR++D + R AP GE D RPEIDR LR LLL Sbjct: 61 HAGLLEEFRPLSRPEDGELRLLDKDAAVGFRSPAPEPGERDEGKERPEIDRRALRDLLLG 120 Query: 117 PLGGNVVHWAAQIRALTVLPDGRVTLQLESGSVGPFDLVIGADGAWSKVRTVLTDIFPAY 176 L V W ++RA+ + GR TL L G DL++GADGAWS+VR +++D P Y Sbjct: 121 SLPEGTVRWGHKVRAVALGVAGRPTLTLSDGRTVETDLLVGADGAWSRVRPLVSDADPVY 180 Query: 177 TGVTFVELWFDDVDQAHPAAAELVGHGTMFALRDQAGIIGQRNGKGRLRVYAAFRTRAED 236 +GV++V+ DVD HPA AELVG GTMFAL D+ G+I QRNG R+RVYAA + E Sbjct: 181 SGVSYVDCLLHDVDARHPAVAELVGRGTMFALADEKGLIAQRNGGDRIRVYAAAKAPEEW 240 Query: 237 GDRPDKTPAN--LGKDELLARFPGWAPSLTSLLRDADHIAAIRPIVTLPAQCRWQHQSGL 294 PA+ + LL FP W L +L+ D+D RPI LP RW G+ Sbjct: 241 SRSGLIDPADPVAARKRLLDLFPDWDEGLRALIADSDGPLVPRPIHALPIGHRWDRTPGV 300 Query: 295 TLVGDAAHVMPPL-GTGVNLAMLDAAELAENLIHAADWRLAVQASERVMLDRSSAIASQC 353 TLVGDAAH+M P G G NLAMLD AELA L D A+ A E M R+ A+Q Sbjct: 301 TLVGDAAHLMSPFAGAGANLAMLDGAELALALTTHNDLETALNAYETPMFPRAEEAAAQS 360 Query: 354 IASFDAMFGPDAHQSGVGHFDA 375 + F PD + F A Sbjct: 361 ADNLTGFFRPDGLRIACDTFTA 382 >ref|NP_624801.1| monooxygenase [Streptomyces coelicolor A3(2)] ref|ZP_06533227.1| monooxygenase [Streptomyces lividans TK24] emb|CAB53314.1| putative monooxygenase [Streptomyces coelicolor A3(2)] gb|EFD71477.1| monooxygenase [Streptomyces lividans TK24] Length = 388 Score = 283 bits (723), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 169/348 (48%), Positives = 211/348 (60%), Gaps = 7/348 (2%) Query: 7 IAIIGAGPAGLTAANVFHRHGWQCEVFESDASAASRDQGGSLDLHPEDGQLALKKAGLFA 66 I+IIGAGP GLT A V RHG V++ D AASRDQGGSLDLH EDGQLAL++AGL Sbjct: 11 ISIIGAGPGGLTCARVLRRHGIAVTVYDRDPDAASRDQGGSLDLHEEDGQLALREAGLLE 70 Query: 67 QFMAVARHEDQGLRIIDGASGAVMREFAPAEGEGDRPEIDRDVLRQLLLEPLGGNVVHWA 126 +F A+AR E Q R D A G ++ P EGE RPEIDR LR LLLE L V W Sbjct: 71 EFFALARCESQEERRFDTA-GRLLGRRLPDEGETARPEIDRGQLRGLLLESLDAGTVRWG 129 Query: 127 AQIRALTVLPDGRVTLQLESGSVGPFDLVIGADGAWSKVRTVLTDIFPAYTGVTFVELWF 186 + +++ +G TL GS DLVIGADGA+S+VR ++D P YTGV F+E WF Sbjct: 130 HGLESVSGPAEGPRTLTFTDGSTVETDLVIGADGAFSRVRAAVSDAVPRYTGVGFLEAWF 189 Query: 187 DDVDQAHPAAAELVGHGTMFALRDQAGIIGQRNGKGRLRVYAAFRTRAE----DGDRPDK 242 DD++ AHP +ELVG G+ Q G+ QRN G +RVY R + G RPD Sbjct: 190 DDMESAHPELSELVGRGSAHVADGQRGLFAQRNSGGHMRVYVMRRVALDWMTASGLRPDD 249 Query: 243 TPANLGKDELLARFPGWAPSLTSLLRDADHIAAIRPIVTLPAQCRWQHQSGLTLVGDAAH 302 T + LLA + GW+P + ++ + D RP+ LP W+ G+TL+GDAAH Sbjct: 250 TDGI--RARLLAEYAGWSPRILRMITENDGPYVDRPLFALPVPHTWRPTPGVTLLGDAAH 307 Query: 303 VMPPLGTGVNLAMLDAAELAENLIHAADWRLAVQASERVMLDRSSAIA 350 +MPPLG GVNLAMLD AELA L +A AV+ E+ ML RS+ IA Sbjct: 308 LMPPLGVGVNLAMLDGAELALALAASATVDDAVRTYEKTMLPRSAEIA 355