BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0203.p (556 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001566307.1| 2-isopropylmalate synthase [Delftia acidovor... 813 0.0 ref|YP_003881368.1| 2-isopropylmalate synthase [Dickeya dadantii... 812 0.0 ref|YP_261220.1| 2-isopropylmalate synthase [Pseudomonas fluores... 812 0.0 ref|YP_004486707.1| 2-isopropylmalate synthase [Delftia sp. Cs1-... 812 0.0 ref|YP_003017906.1| 2-isopropylmalate synthase [Pectobacterium c... 809 0.0 >ref|YP_001566307.1| 2-isopropylmalate synthase [Delftia acidovorans SPH-1] gb|ABX37922.1| 2-isopropylmalate synthase [Delftia acidovorans SPH-1] Length = 566 Score = 813 bits (2100), Expect = 0.0, Method: Compositional matrix adjust. Identities = 407/556 (73%), Positives = 456/556 (82%), Gaps = 6/556 (1%) Query: 1 MIAHSRTKYPHPPTVDLPDRQWPSRTIGKAPIWLSTDLRDGNQALFEPMNAERKLTLFHE 60 M+ + ++Y P+VDLP+RQWPSR + +AP+WLSTDLRDGNQALFEPMN ERKL LFHE Sbjct: 1 MLKNPASRYLTFPSVDLPERQWPSRQLSQAPVWLSTDLRDGNQALFEPMNRERKLRLFHE 60 Query: 61 LVRLGFKEIEVGFPAGSQTDFDVVRTLITDKHIPADVTPMVMTQLRGEQIVRTVES-HGA 119 LVR+GFKEIEVGFP+ SQTD+ +VR LI + IP DVTPMV+TQLR + I TV+S GA Sbjct: 61 LVRIGFKEIEVGFPSASQTDYGIVRHLIDNGLIPGDVTPMVITQLREDLIRATVDSVAGA 120 Query: 120 PRAIVHFYNAVAPLWREVVFGLSVPQVMQLIERNVRLLKDLTAQHPETEWILQYSPETFC 179 RAIVHFYNA+AP WRE+VFG+ VP+++ ++E ++ LL++LT HPETEW+LQYSPETFC Sbjct: 121 RRAIVHFYNAIAPAWREIVFGMQVPEIIAMVEHHIALLRELTDAHPETEWVLQYSPETFC 180 Query: 180 MAELEVSLQACRVAIDTWDAGPDRPIIINLPTTVEVSTPNVFADQIEWMHRRLPRREHVV 239 MAEL+VSL C AI WDAGP RP+IINLPTTVEVST NVFADQIEWMHRRL RREH+V Sbjct: 181 MAELDVSLAVCNAAIRAWDAGPARPMIINLPTTVEVSTANVFADQIEWMHRRLERREHIV 240 Query: 240 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDVVTLALNLYTNGIDPQLD 299 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDVVTLALNLYT GI P LD Sbjct: 241 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDVVTLALNLYTQGIAPGLD 300 Query: 300 FSDIAAVARVAENCTALPIHPRHPYVGDLVFTAFSGSHQDAIRKGFAAQKPDGLWRVPYL 359 FSDIAAVAR+AE CTALPIHPRHPYVGDLVFTAFSGSHQDAI KGFA Q+PD WRVPYL Sbjct: 301 FSDIAAVARIAEECTALPIHPRHPYVGDLVFTAFSGSHQDAIAKGFAVQRPDAPWRVPYL 360 Query: 360 PIDPQDIGRTYDSIVRVNSQSGKGGIAFLLERDHGIVMPRRMQVEFSAIVQKHADASETE 419 PIDPQD+GRTYDSIVRVNSQSGKGGIAFLL+RDHG+ MPRRMQVEFSA+VQK ADA E E Sbjct: 361 PIDPQDLGRTYDSIVRVNSQSGKGGIAFLLQRDHGVTMPRRMQVEFSAVVQKQADACEAE 420 Query: 420 ISSAALWELFERTYLASPRQDGRVRYLSHQLSNPGAAQDIALELT-VSGVAIRLNGNGNG 478 ++S LW+LFE TYLA P Q Y SH L + Q IALE+ G+ ++L G G+G Sbjct: 421 LASDDLWKLFEATYLA-PLQLPW-HYRSHHLFDSAQGQGIALEVVRPDGLRLQLYGEGSG 478 Query: 479 LIAATVDALRSRLTGLEGLRIDSYEERSIGSGAQAQAWAIVEAASPGVAGTRFGAARHVN 538 I A AL L LRID YEERS+G GA A A AIVEAA PGVAGTRFGA RH N Sbjct: 479 PIDAVAAALGQHLG--VALRIDHYEERSLGQGAGAMALAIVEAALPGVAGTRFGAGRHAN 536 Query: 539 IVTASILAVLSAANRL 554 IVTASILAVL AA RL Sbjct: 537 IVTASILAVLHAAGRL 552 >ref|YP_003881368.1| 2-isopropylmalate synthase [Dickeya dadantii 3937] gb|ADM96811.1| 2-isopropylmalate synthase [Dickeya dadantii 3937] Length = 567 Score = 812 bits (2098), Expect = 0.0, Method: Compositional matrix adjust. Identities = 412/564 (73%), Positives = 454/564 (80%), Gaps = 14/564 (2%) Query: 1 MIAHSRTKYPHPPTVDLPDRQWPSRTIGKAPIWLSTDLRDGNQALFEPMNAERKLTLFHE 60 M+ KY P V+L DRQWPSRT+ +APIWLSTDLRDGNQALFEPMN ERKL LFHE Sbjct: 4 MLIDPSQKYRPAPLVNLVDRQWPSRTLTQAPIWLSTDLRDGNQALFEPMNRERKLRLFHE 63 Query: 61 LVRLGFKEIEVGFPAGSQTDFDVVRTLITDKHIPADVTPMVMTQLRGEQIVRTVES-HGA 119 LVRLGFKEIEVGFPA S+TDF++VR LI + IP DVTPMVMTQLR + I TV GA Sbjct: 64 LVRLGFKEIEVGFPAASRTDFEIVRHLIDTEQIPEDVTPMVMTQLREDLIAETVRCVAGA 123 Query: 120 PRAIVHFYNAVAPLWREVVFGLSVPQVMQLIERNVRLLKDLTAQHPETEWILQYSPETFC 179 R IVHFYNA+AP+WRE VFG+SVPQ++ ++ER++ L K LTA HPETEW+LQYSPETFC Sbjct: 124 RRVIVHFYNAIAPVWRETVFGMSVPQIIDMVERHIALFKRLTAAHPETEWVLQYSPETFC 183 Query: 180 MAELEVSLQACRVAIDTWDAGPDRPIIINLPTTVEVSTPNVFADQIEWMHRRLPRREHVV 239 MAEL+VSL C AI WDAGP R +IINLPTTVEVSTPNVFADQIEWM RRL RREH+V Sbjct: 184 MAELDVSLSVCNAAIHAWDAGPQRTMIINLPTTVEVSTPNVFADQIEWMDRRLARREHIV 243 Query: 240 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDVVTLALNLYTNGIDPQLD 299 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGN+DVVTLALNLYT GI P LD Sbjct: 244 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNVDVVTLALNLYTQGIAPGLD 303 Query: 300 FSDIAAVARVAENCTALPIHPRHPYVGDLVFTAFSGSHQDAIRKGFA------AQKPDGL 353 FSDIAAVARVAE CTALPIHPRHPYVGDLVFTAFSGSHQDAI KGFA AQ+ D Sbjct: 304 FSDIAAVARVAEECTALPIHPRHPYVGDLVFTAFSGSHQDAIAKGFAAQYSQLAQQADKP 363 Query: 354 WRVPYLPIDPQDIGRTYDSIVRVNSQSGKGGIAFLLERDHGIVMPRRMQVEFSAIVQKHA 413 W VPYLPIDP+D+GRTYDSIVRVNSQSGKGGIAFLL+RDHGIVMPRRMQVEFSAIVQ A Sbjct: 364 WCVPYLPIDPKDLGRTYDSIVRVNSQSGKGGIAFLLQRDHGIVMPRRMQVEFSAIVQALA 423 Query: 414 DASETEISSAALWELFERTYLASPRQDGRVRYLSHQLSNPGAAQDIALELTVSGVAIRL- 472 DASETE++S +W LFERTYL ++ Y H+L + Q IALEL + +R+ Sbjct: 424 DASETELASEQIWALFERTYLVPMQEQPGFIYRHHRLFDHTDGQGIALELVHTDSDVRIV 483 Query: 473 NGNGNGLIAATVDALRSRLTGLEGLRIDSYEERSIGSGAQAQAWAIVEAASPGVAGTRFG 532 G GNG IAATV AL GL LRIDSYEERS+G+G+ A A AIVEAA PGV G+RFG Sbjct: 484 EGTGNGPIAATVAAL-----GLP-LRIDSYEERSLGAGSNAVALAIVEAAIPGVPGSRFG 537 Query: 533 AARHVNIVTASILAVLSAANRLQS 556 RH NI TASILAVLSAA R + Sbjct: 538 VGRHTNITTASILAVLSAAARFSA 561 >ref|YP_261220.1| 2-isopropylmalate synthase [Pseudomonas fluorescens Pf-5] gb|AAY93384.1| putative 2-isopropylmalate synthase [Pseudomonas fluorescens Pf-5] Length = 571 Score = 812 bits (2098), Expect = 0.0, Method: Compositional matrix adjust. Identities = 412/556 (74%), Positives = 456/556 (82%), Gaps = 8/556 (1%) Query: 1 MIAHSRTKYPHPPTVDLPDRQWPSRTIGKAPIWLSTDLRDGNQALFEPMNAERKLTLFHE 60 M+AH TKY VDL RQWPSRTI +APIWLSTDLRDGNQALFEPMN ERKL LF E Sbjct: 1 MLAHPHTKYRPFTPVDLDSRQWPSRTITQAPIWLSTDLRDGNQALFEPMNRERKLRLFEE 60 Query: 61 LVRLGFKEIEVGFPAGSQTDFDVVRTLITDKHIPADVTPMVMTQLRGEQIVRTVES-HGA 119 LVRLGFKEIEVGFPA S+TDF+VVR L+ + IPADVTPMVMTQLR + I TV S GA Sbjct: 61 LVRLGFKEIEVGFPAASRTDFEVVRELVEQQRIPADVTPMVMTQLREDLIRETVRSVQGA 120 Query: 120 PRAIVHFYNAVAPLWREVVFGLSVPQVMQLIERNVRLLKDLTAQHPETEWILQYSPETFC 179 R IVH YNA+AP+WR VVFGLSV +V L+ER+VRLL++LT QHPETEW+LQYSPETFC Sbjct: 121 RRVIVHLYNAIAPVWRRVVFGLSVDEVEALVERHVRLLRELTDQHPETEWVLQYSPETFC 180 Query: 180 MAELEVSLQACRVAIDTWDAGPDRPIIINLPTTVEVSTPNVFADQIEWMHRRLPRREHVV 239 MAELEVSL+ C AI TWDAGP RPIIINLPTTVEV+T N+FADQIEWMH+RL RR+ V Sbjct: 181 MAELEVSLRMCNTAIRTWDAGPGRPIIINLPTTVEVATANIFADQIEWMHKRLERRDQVT 240 Query: 240 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDVVTLALNLYTNGIDPQLD 299 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLD+VTLALNLYT GI P LD Sbjct: 241 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDLVTLALNLYTQGISPGLD 300 Query: 300 FSDIAAVARVAENCTALPIHPRHPYVGDLVFTAFSGSHQDAIRKGFAAQKPDGLWRVPYL 359 FSDIAAVARVAE CTALPIHPRHPYVGDLVFTAFSGSHQDAI KGFAAQ DG W VPYL Sbjct: 301 FSDIAAVARVAEECTALPIHPRHPYVGDLVFTAFSGSHQDAIAKGFAAQAADGFWEVPYL 360 Query: 360 PIDPQDIGRTYDSIVRVNSQSGKGGIAFLLERDHGIVMPRRMQVEFSAIVQKHADASETE 419 PIDPQD+GRTYDSIVRVNSQSGKGGIAFLL+RDHGIVMPRRMQ+EFSAIVQK ADAS+ E Sbjct: 361 PIDPQDLGRTYDSIVRVNSQSGKGGIAFLLQRDHGIVMPRRMQIEFSAIVQKLADASDAE 420 Query: 420 ISSAALWELFERTYLASPRQDGRVRYLSHQLSNPGAAQDIALELTVSG-VAIRLNGNGNG 478 +SS ALWE+F+RTYLA +RYL+H+L + Q I L + G L+G GNG Sbjct: 421 LSSDALWEVFQRTYLACAEGTAGLRYLAHRLFDAPEGQGIELRVATPGNQEACLHGVGNG 480 Query: 479 LIAATVDALRSRLTGLEGLRIDSYEERSIGSGAQAQAWAIVEAASPGVAGTRFGAARHVN 538 +AA + AL LRIDS+EER++G+GA AQA AIVEA PGVAG+RFGA H N Sbjct: 481 PVAAALAALGLP------LRIDSFEERNLGAGADAQALAIVEAVWPGVAGSRFGAGCHAN 534 Query: 539 IVTASILAVLSAANRL 554 IVTAS+ AV++AA R Sbjct: 535 IVTASVQAVINAAARF 550 >ref|YP_004486707.1| 2-isopropylmalate synthase [Delftia sp. Cs1-4] gb|AEF88352.1| 2-isopropylmalate synthase [Delftia sp. Cs1-4] Length = 566 Score = 812 bits (2097), Expect = 0.0, Method: Compositional matrix adjust. Identities = 407/556 (73%), Positives = 455/556 (81%), Gaps = 6/556 (1%) Query: 1 MIAHSRTKYPHPPTVDLPDRQWPSRTIGKAPIWLSTDLRDGNQALFEPMNAERKLTLFHE 60 M+ + ++Y P VDLP+RQWPSR + +AP+WLSTDLRDGNQALFEPMN ERKL LFHE Sbjct: 1 MLKNPASRYRPFPAVDLPERQWPSRQLSQAPVWLSTDLRDGNQALFEPMNRERKLRLFHE 60 Query: 61 LVRLGFKEIEVGFPAGSQTDFDVVRTLITDKHIPADVTPMVMTQLRGEQIVRTVES-HGA 119 LVR+GFKEIEVGFP+ SQTD+ +VR LI + IP DVTPMV+TQLR + I TV+S GA Sbjct: 61 LVRIGFKEIEVGFPSASQTDYGIVRHLIDNGLIPGDVTPMVITQLREDLIRATVDSVAGA 120 Query: 120 PRAIVHFYNAVAPLWREVVFGLSVPQVMQLIERNVRLLKDLTAQHPETEWILQYSPETFC 179 RAIVHFYNA+AP WRE+VFG+ VP+++ ++E ++ LL++LT HPETEW+LQYSPETFC Sbjct: 121 RRAIVHFYNAIAPAWREIVFGMQVPEIIAMVEHHIALLRELTDAHPETEWVLQYSPETFC 180 Query: 180 MAELEVSLQACRVAIDTWDAGPDRPIIINLPTTVEVSTPNVFADQIEWMHRRLPRREHVV 239 MAEL+VSL C AI WDAGP RP+IINLPTTVEVST NVFADQIEWMHRRL RREH+V Sbjct: 181 MAELDVSLAVCNAAIRAWDAGPARPMIINLPTTVEVSTANVFADQIEWMHRRLERREHIV 240 Query: 240 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDVVTLALNLYTNGIDPQLD 299 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDVVTLALNLYT GI P LD Sbjct: 241 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDVVTLALNLYTQGIAPGLD 300 Query: 300 FSDIAAVARVAENCTALPIHPRHPYVGDLVFTAFSGSHQDAIRKGFAAQKPDGLWRVPYL 359 FSDIAAVAR+AE CTALPIHPRHPYVGDLVFTAFSGSHQDAI KGFAAQ+PD WRVPYL Sbjct: 301 FSDIAAVARIAEECTALPIHPRHPYVGDLVFTAFSGSHQDAIAKGFAAQRPDAPWRVPYL 360 Query: 360 PIDPQDIGRTYDSIVRVNSQSGKGGIAFLLERDHGIVMPRRMQVEFSAIVQKHADASETE 419 PIDPQD+GRTYDSIVRVNSQSGKGGIAFLL+RDH + MPRRMQVEFSA+VQK ADA E E Sbjct: 361 PIDPQDLGRTYDSIVRVNSQSGKGGIAFLLQRDHSVTMPRRMQVEFSAVVQKQADACEAE 420 Query: 420 ISSAALWELFERTYLASPRQDGRVRYLSHQLSNPGAAQDIALELT-VSGVAIRLNGNGNG 478 ++S LW+LFE TYLA P Q Y SH L + Q IALE+ G+ ++L G G+G Sbjct: 421 LASDDLWKLFEATYLA-PLQLP-WHYRSHHLFDSAQGQGIALEVVRPDGLRLQLYGEGSG 478 Query: 479 LIAATVDALRSRLTGLEGLRIDSYEERSIGSGAQAQAWAIVEAASPGVAGTRFGAARHVN 538 I A AL L LRID YEERS+G GA A A AIVEAA PGVAGTRFGA RH N Sbjct: 479 PIDAVAAALGQHLG--VALRIDHYEERSLGQGAGAMALAIVEAALPGVAGTRFGAGRHAN 536 Query: 539 IVTASILAVLSAANRL 554 IVTASILAVL AA RL Sbjct: 537 IVTASILAVLHAAGRL 552 >ref|YP_003017906.1| 2-isopropylmalate synthase [Pectobacterium carotovorum subsp. carotovorum PC1] gb|ACT13370.1| 2-isopropylmalate synthase [Pectobacterium carotovorum subsp. carotovorum PC1] Length = 558 Score = 809 bits (2090), Expect = 0.0, Method: Compositional matrix adjust. Identities = 406/556 (73%), Positives = 452/556 (81%), Gaps = 8/556 (1%) Query: 1 MIAHSRTKYPHPPTVDLPDRQWPSRTIGKAPIWLSTDLRDGNQALFEPMNAERKLTLFHE 60 M+ KY P VDL DRQWPSRT+ +AP+WLSTDLRDGNQALFEPMN ERKL LF E Sbjct: 1 MLTDPSQKYRPFPAVDLVDRQWPSRTLTRAPVWLSTDLRDGNQALFEPMNHERKLHLFQE 60 Query: 61 LVRLGFKEIEVGFPAGSQTDFDVVRTLITDKHIPADVTPMVMTQLRGEQIVRTVES-HGA 119 LVR+GFKEIEVGFP+ S+TDF+VVR LI IP DVTPMV+TQLR + I TV S GA Sbjct: 61 LVRIGFKEIEVGFPSASRTDFEVVRHLIDADLIPDDVTPMVITQLRDDLIEETVRSVAGA 120 Query: 120 PRAIVHFYNAVAPLWREVVFGLSVPQVMQLIERNVRLLKDLTAQHPETEWILQYSPETFC 179 R IVHFYNA+AP+WRE+VFG+SV Q+++ +E + LL+ LTA+HP+TEW+LQYSPETFC Sbjct: 121 RRVIVHFYNAIAPVWREIVFGMSVEQIIEKVEHAIALLRQLTAEHPQTEWVLQYSPETFC 180 Query: 180 MAELEVSLQACRVAIDTWDAGPDRPIIINLPTTVEVSTPNVFADQIEWMHRRLPRREHVV 239 MAELEVSL C AI WDAGP RP+IINLPTTVEVSTPNVFADQIEWMHRRL RREH+V Sbjct: 181 MAELEVSLAVCNAAIQAWDAGPQRPMIINLPTTVEVSTPNVFADQIEWMHRRLARREHIV 240 Query: 240 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDVVTLALNLYTNGIDPQLD 299 LSVHPHNDRGTG+ACAE ALLAGAQRVEGCLFGNGERSGNLDVVT+ALNLYT GI P LD Sbjct: 241 LSVHPHNDRGTGIACAELALLAGAQRVEGCLFGNGERSGNLDVVTMALNLYTQGIPPHLD 300 Query: 300 FSDIAAVARVAENCTALPIHPRHPYVGDLVFTAFSGSHQDAIRKGFAAQKPDGLWRVPYL 359 FSDIAAVARVAE+CTALPIHPRHPYVGDL+FTAFSGSHQDAI KGFA Q+ D LWRVPYL Sbjct: 301 FSDIAAVARVAEDCTALPIHPRHPYVGDLIFTAFSGSHQDAIAKGFATQRADALWRVPYL 360 Query: 360 PIDPQDIGRTYDSIVRVNSQSGKGGIAFLLERDHGIVMPRRMQVEFSAIVQKHADASETE 419 PIDPQD+GR YDSI+RVNSQSGKGGIAFLL+RDHGIVMPRRMQVEFSAIVQ ADASETE Sbjct: 361 PIDPQDVGRNYDSIIRVNSQSGKGGIAFLLQRDHGIVMPRRMQVEFSAIVQTLADASETE 420 Query: 420 ISSAALWELFERTYLASPRQDGRVRYLSHQLSNPGAAQDIALELT-VSGVAIRLNGNGNG 478 +SS +WE+FE+TYLA + Y +H+LS Q I L L G G GNG Sbjct: 421 LSSERIWEIFEQTYLAPVFEQPGFIYNAHRLSEHPQGQGIDLTLIDADGEPRYCRGEGNG 480 Query: 479 LIAATVDALRSRLTGLEGLRIDSYEERSIGSGAQAQAWAIVEAASPGVAGTRFGAARHVN 538 I+ATV AL GL LRIDSYEERS+G+GA A A AIVEA PGV+G+RFG RH N Sbjct: 481 PISATVAAL-----GLP-LRIDSYEERSLGAGADAVALAIVEAVRPGVSGSRFGVGRHPN 534 Query: 539 IVTASILAVLSAANRL 554 IVTAS+LAVLSAA R Sbjct: 535 IVTASVLAVLSAAARF 550