BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0197.p (312 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001101619.1| putative thiamine biosynthesis protein ApbE ... 339 4e-91 ref|YP_842013.1| protein involved in thiamine biosynthesis [Rals... 338 9e-91 ref|YP_001355306.1| thiamine biosynthesis lipoprotein [Janthinob... 337 1e-90 ref|YP_002008845.1| thiamine biosynthesis lipoprotein; apbe fami... 335 4e-90 ref|YP_004682579.1| thiamine biosynthesis protein [Cupriavidus n... 327 2e-87 >ref|YP_001101619.1| putative thiamine biosynthesis protein ApbE [Herminiimonas arsenicoxydans] emb|CAL63498.1| putative Thiamine biosynthesis lipoprotein ApbE-like precursor [Herminiimonas arsenicoxydans] Length = 344 Score = 339 bits (869), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 179/320 (55%), Positives = 221/320 (69%), Gaps = 15/320 (4%) Query: 1 MGTSWSVKLIRPPHLALETIRDGIQQQLDLVIGQMSTWSASSDLSRYNQSEAGSWQALPV 60 MGTSWSVKL L +++ IQ QLD VI QMSTW SDLSRYNQ+ AGSW LP Sbjct: 30 MGTSWSVKLAADAARRLLPLQELIQHQLDTVIAQMSTWETDSDLSRYNQAAAGSWHRLPD 89 Query: 61 PSVPPVHFLRVLDCALDVAERSGGAYDPSIGPLVNLWGFGPDPARSE----APDAAALAQ 116 F RVL+ ALDVA+ S GAYDP+ G LVN WGFGP +E P AL Sbjct: 90 ------EFFRVLEYALDVAQASDGAYDPTAGALVNAWGFGPTQRYTEEGFVVPGNTALDF 143 Query: 117 AQARCGWQRVRIDRARNALLQPGGLYLDFSSIAKGYAVDLVAEYLRGIGVSSHLVEIGGE 176 A+A+CGWQR+++D+A + +LQPG +D S+IAKG++VD +A L G+ S LVE+GGE Sbjct: 144 ARAQCGWQRIQLDKAAHRVLQPGNATIDLSAIAKGFSVDQIARALLQRGIDSFLVEVGGE 203 Query: 177 LRGHGVKADGQPWWVGLEQP-PAPTG----SESGMQSVVALHDLALATSGDYRRYFEHAG 231 LRG GVK DGQPWWV LE P PA +E+ + ++ AL++L++ATSGDYRRYFE Sbjct: 204 LRGAGVKPDGQPWWVALEHPLPASFADAITNEASIATIAALYNLSVATSGDYRRYFEEGD 263 Query: 232 TRYSHTIDPRTAQPIAHALASVTVLHADCMTADAWATALMVLGDEDGLALALEVGLAALF 291 TR+SHTIDPR+ +PIAH LASVTVLH DCM ADAW+TAL V+G GLA A + LAALF Sbjct: 264 TRFSHTIDPRSGEPIAHGLASVTVLHCDCMAADAWSTALGVMGAAQGLAFADQHDLAALF 323 Query: 292 VRRDGAGFRESMTPAMQAML 311 ++R GF E M+ AM A+L Sbjct: 324 IQRGKEGFEEHMSQAMAALL 343 >ref|YP_842013.1| protein involved in thiamine biosynthesis [Ralstonia eutropha H16] emb|CAJ97283.1| protein involved in thiamine biosynthesis [Ralstonia eutropha H16] Length = 338 Score = 338 bits (866), Expect = 9e-91, Method: Compositional matrix adjust. Identities = 178/312 (57%), Positives = 211/312 (67%), Gaps = 7/312 (2%) Query: 1 MGTSWSVKLIRPPHLALETIRDGIQQQLDLVIGQMSTWSASSDLSRYNQSEAGSWQALPV 60 MGT+WSV + PP + I GIQ LD VI QMS W A SDLSR+N + GSW ALP Sbjct: 34 MGTTWSVAALLPPATDADAIAAGIQAVLDGVIAQMSNWEADSDLSRFNGAAPGSWHALPE 93 Query: 61 PSVPPVHFLRVLDCALDVAERSGGAYDPSIGPLVNLWGFGPDPARSEAPDAAALAQAQAR 120 VL CAL VA SGGAYDPS GPLV+LWGFGP PAR+ P A+ + R Sbjct: 94 ------DCFMVLQCALLVARDSGGAYDPSAGPLVDLWGFGPAPARTAPPSPDAVQATRGR 147 Query: 121 CGWQRVRIDRARNALLQPGGLYLDFSSIAKGYAVDLVAEYLRGIGVSSHLVEIGGELRGH 180 CGW R+ +D QPGG+ LDF +IAKG+AVD VA YL G+ HL EIGGELRGH Sbjct: 148 CGWARIEVDATGRRAWQPGGVSLDFCAIAKGFAVDAVAGYLCEQGLDHHLTEIGGELRGH 207 Query: 181 GVKADGQPWWVGLEQPPAPTGSESGMQSVVALHDLALATSGDYRRYFEHAGTRYSHTIDP 240 GVK DG PWWV LE PP GS++ Q++VALH L++ATSGDYRRYF+ G RY+HTIDP Sbjct: 208 GVKPDGMPWWVALEAPPG-LGSDAAGQTLVALHGLSVATSGDYRRYFDSDGRRYAHTIDP 266 Query: 241 RTAQPIAHALASVTVLHADCMTADAWATALMVLGDEDGLALALEVGLAALFVRRDGAGFR 300 RT P +HALASVTV+HA CM ADA +TAL VLG + G+A A G+AA F+ R GF Sbjct: 267 RTGYPASHALASVTVIHAQCMLADALSTALTVLGPQAGMAHARRHGIAARFLVRTPGGFD 326 Query: 301 ESMTPAMQAMLS 312 E ++PA AML+ Sbjct: 327 EQLSPAFAAMLA 338 >ref|YP_001355306.1| thiamine biosynthesis lipoprotein [Janthinobacterium sp. Marseille] gb|ABR89002.1| thiamine biosynthesis lipoprotein [Janthinobacterium sp. Marseille] Length = 345 Score = 337 bits (864), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 175/321 (54%), Positives = 215/321 (66%), Gaps = 16/321 (4%) Query: 1 MGTSWSVKLIRPPHLALETIRDGIQQQLDLVIGQMSTWSASSDLSRYNQSEAGSWQALPV 60 MGTSWSVKL+ L +R IQQQLD V+ QMSTW A+S+LS YNQ+ AGSW LP Sbjct: 30 MGTSWSVKLVAEVTRRLPPLRAAIQQQLDTVVAQMSTWEATSNLSLYNQAAAGSWHTLPD 89 Query: 61 PSVPPVHFLRVLDCALDVAERSGGAYDPSIGPLVNLWGFGPDPARSEA----PDAAALAQ 116 F VL AL VA S GAYDPS G LVN WGFGP +E P L Q Sbjct: 90 ------EFFTVLQYALKVARASNGAYDPSAGALVNAWGFGPCKRYNETDFSTPTPDVLEQ 143 Query: 117 AQARCGWQRVRIDRARNALLQPGGLYLDFSSIAKGYAVDLVAEYLRGIGVSSHLVEIGGE 176 A+ +CGWQR+ +D + +LQ GG+Y+D S+IAKG+ VD VA L +G+ S LVE+GGE Sbjct: 144 ARKQCGWQRIELDSSTKRVLQAGGMYIDLSAIAKGFGVDQVARELLRLGIDSFLVEVGGE 203 Query: 177 LRGHGVKADGQPWWVGLEQP------PAPTGSESGMQSVVALHDLALATSGDYRRYFEHA 230 LRG GVK DGQPWWV LE P A + + G++++ AL+D + ATSGDYRRYFE+ Sbjct: 204 LRGAGVKPDGQPWWVALEHPLPDALASAASNTAVGIETIAALYDWSAATSGDYRRYFEND 263 Query: 231 GTRYSHTIDPRTAQPIAHALASVTVLHADCMTADAWATALMVLGDEDGLALALEVGLAAL 290 RYSHTIDPR+ +PI H LASVTVLH DCM ADAW+TAL V+G E GLA A + LAAL Sbjct: 264 TKRYSHTIDPRSGEPIRHGLASVTVLHKDCMAADAWSTALGVMGAEQGLAYANQHDLAAL 323 Query: 291 FVRRDGAGFRESMTPAMQAML 311 F+ R+ GF E ++ A+ A+L Sbjct: 324 FINRNKDGFEEHLSSALVALL 344 >ref|YP_002008845.1| thiamine biosynthesis lipoprotein; apbe family [Cupriavidus taiwanensis LMG 19424] emb|CAQ72793.1| Thiamine biosynthesis lipoprotein; apbE family [Cupriavidus taiwanensis LMG 19424] Length = 335 Score = 335 bits (860), Expect = 4e-90, Method: Compositional matrix adjust. Identities = 179/312 (57%), Positives = 211/312 (67%), Gaps = 7/312 (2%) Query: 1 MGTSWSVKLIRPPHLALETIRDGIQQQLDLVIGQMSTWSASSDLSRYNQSEAGSWQALPV 60 MGTSWSV + PP I GI+ L VI QMS W A SDLSR+N++ +GSW ALP Sbjct: 31 MGTSWSVAALLPPDADAGAIGAGIRAVLGRVIAQMSNWEADSDLSRFNRAPSGSWHALPA 90 Query: 61 PSVPPVHFLRVLDCALDVAERSGGAYDPSIGPLVNLWGFGPDPARSEAPDAAALAQAQAR 120 + VL AL VA SGGAYDPS GPLV+LWGFGP PAR P + A+AR Sbjct: 91 DA------FAVLQAALQVARDSGGAYDPSAGPLVDLWGFGPAPARRAPPSPDDVQAARAR 144 Query: 121 CGWQRVRIDRARNALLQPGGLYLDFSSIAKGYAVDLVAEYLRGIGVSSHLVEIGGELRGH 180 CGW R+ ID A QPGG+ LDF +IAKG+AVD VA YL G+ HL EIGGELRGH Sbjct: 145 CGWARIEIDAAGRRAWQPGGVALDFCAIAKGFAVDAVARYLCEQGLEHHLTEIGGELRGH 204 Query: 181 GVKADGQPWWVGLEQPPAPTGSESGMQSVVALHDLALATSGDYRRYFEHAGTRYSHTIDP 240 GVK DG PWWV LE PP GS+ Q++VALH L++ATSGDYRRYF+ G RY+HTIDP Sbjct: 205 GVKPDGMPWWVALEAPPG-WGSDDAQQTLVALHGLSVATSGDYRRYFDSDGRRYAHTIDP 263 Query: 241 RTAQPIAHALASVTVLHADCMTADAWATALMVLGDEDGLALALEVGLAALFVRRDGAGFR 300 RT P +HALASVTV+H CM ADA +TAL VLG + G+A A +G+AA FV R GF Sbjct: 264 RTGYPASHALASVTVIHPQCMLADALSTALTVLGPDAGMAHARRLGIAARFVVRTANGFL 323 Query: 301 ESMTPAMQAMLS 312 E ++PA AML+ Sbjct: 324 EHLSPAFAAMLA 335 >ref|YP_004682579.1| thiamine biosynthesis protein [Cupriavidus necator N-1] gb|AEI81347.1| protein involved in thiamine biosynthesis [Cupriavidus necator N-1] Length = 338 Score = 327 bits (837), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 178/312 (57%), Positives = 209/312 (66%), Gaps = 7/312 (2%) Query: 1 MGTSWSVKLIRPPHLALETIRDGIQQQLDLVIGQMSTWSASSDLSRYNQSEAGSWQALPV 60 MGT+WSV + PP I GIQ LD VI QMS W A SDLSR+N++ G+W ALP Sbjct: 34 MGTTWSVAALLPPAADAHGIAAGIQAVLDGVITQMSNWEADSDLSRFNRAAPGNWHALPD 93 Query: 61 PSVPPVHFLRVLDCALDVAERSGGAYDPSIGPLVNLWGFGPDPARSEAPDAAALAQAQAR 120 VL CAL VA SGGAYDPS GPLV+LWGFGP PARS P A+ ++R Sbjct: 94 ------DCFAVLQCALQVARDSGGAYDPSAGPLVDLWGFGPAPARSAPPSPDAVQATRSR 147 Query: 121 CGWQRVRIDRARNALLQPGGLYLDFSSIAKGYAVDLVAEYLRGIGVSSHLVEIGGELRGH 180 CGW R+ +D A QPGGL LDF +IAKG+AVD VA YL G+ HL EIGGELRGH Sbjct: 148 CGWARIEVDAAGRRARQPGGLSLDFCAIAKGFAVDAVARYLCEQGLDHHLTEIGGELRGH 207 Query: 181 GVKADGQPWWVGLEQPPAPTGSESGMQSVVALHDLALATSGDYRRYFEHAGTRYSHTIDP 240 GVK D PWWV LE PP S++ Q+ VALH L++ATSGDYRRYF+ G RY+HTIDP Sbjct: 208 GVKPDVMPWWVALESPPG-LDSDAAGQTRVALHGLSVATSGDYRRYFDSDGRRYAHTIDP 266 Query: 241 RTAQPIAHALASVTVLHADCMTADAWATALMVLGDEDGLALALEVGLAALFVRRDGAGFR 300 RT P +HALASVTV+H CM ADA +TAL VLG E G+A A G+AA F+ R GF Sbjct: 267 RTGYPASHALASVTVIHGQCMLADALSTALTVLGPEAGMAHARRHGIAARFLVRTPDGFD 326 Query: 301 ESMTPAMQAMLS 312 E ++PA AML+ Sbjct: 327 EHLSPAFAAMLA 338