BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0176.p (365 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_03628450.1| putative DNA topoisomerase I [bacterium Ellin... 416 e-114 ref|YP_001771806.1| putative DNA topoisomerase I [Methylobacteri... 415 e-114 ref|YP_002963036.1| DNA topoisomerase I [methylobacterium extorq... 414 e-114 ref|YP_002421026.1| DNA topoisomerase I [Methylobacterium chloro... 414 e-114 ref|YP_003068256.1| DNA topoisomerase I [Methylobacterium extorq... 413 e-113 >ref|ZP_03628450.1| putative DNA topoisomerase I [bacterium Ellin514] gb|EEF61153.1| putative DNA topoisomerase I [bacterium Ellin514] Length = 367 Score = 416 bits (1069), Expect = e-114, Method: Compositional matrix adjust. Identities = 198/349 (56%), Positives = 264/349 (75%), Gaps = 7/349 (2%) Query: 15 VPESTPTAKQIAREGGLRYVNDDTPGILRSRSDGSDGEFIYTDAHGKTIHDEATLARIKA 74 +P+ +AK I R LRYV DDT GI R ++ G D FI DA GK + DE TL RI + Sbjct: 1 MPDPVESAK-IVR---LRYVTDDTSGISREKA-GDDFRFI--DAEGKRVTDEKTLDRINS 53 Query: 75 MAIPPAWEEVWICPWANGHIQATGRDSRQRKQYRYHARWRIVRDEVKYQRMIAFAQALPQ 134 +AIPPAW EVWICP+ANGH+QATGRD+R+RKQ+RYH +WR +RD+ KY +MIAFA+ALPQ Sbjct: 54 LAIPPAWTEVWICPYANGHLQATGRDARKRKQHRYHPKWREIRDQNKYAQMIAFAKALPQ 113 Query: 135 IRKCLEHDLKKHGLNKQKVLATVVYLLQATLMRVGNDEYARNNQSFGITTLRNRHVKVDG 194 IR+ ++ DL G + KVLATVV LL+ TL+RVGN+EYA+ N+S+G+TTL++RHV + G Sbjct: 114 IRQRVDADLALSGFPRAKVLATVVKLLEVTLIRVGNEEYAKENRSYGLTTLKDRHVDISG 173 Query: 195 ADIKFHFRGKSGVQHSIKLHDPYMVRIVRKLLDLPGQDLFQYLDEDGKTHTIGSGDVNDY 254 + + FHF+GKSG H I +HD + +IV+ + DLPGQ+LFQY+D+DG+ + S DVN Y Sbjct: 174 SKLHFHFKGKSGKNHEIDIHDRKLAKIVKGVQDLPGQELFQYIDDDGQRKQVDSDDVNTY 233 Query: 255 LHEITGEDYTAKDFRTWAGTVLAALALDECRQFDSQAQAKKNIVQAIEHVAKKLGNTPTI 314 L EITGED+TAK FRTWAGT+LAA AL E ++ DS+ +AKKN++QAI+ V+++LGNTPTI Sbjct: 234 LKEITGEDFTAKCFRTWAGTILAAQALQEFKKCDSEVEAKKNVIQAIKSVSQRLGNTPTI 293 Query: 315 CRKCYVHPDVISSYLDGMTAGRLKEQVRDNLKQEMHALTPEEAAVLALL 363 CRKCYVHP ++ Y+D L +Q L + + L+P+EAAVLA+L Sbjct: 294 CRKCYVHPAILELYMDAALVETLTQQAGKVLSESLQELSPQEAAVLAML 342 >ref|YP_001771806.1| putative DNA topoisomerase I [Methylobacterium sp. 4-46] gb|ACA19372.1| putative DNA topoisomerase I [Methylobacterium sp. 4-46] Length = 389 Score = 415 bits (1066), Expect = e-114, Method: Compositional matrix adjust. Identities = 198/354 (55%), Positives = 258/354 (72%), Gaps = 3/354 (0%) Query: 10 DCGPTVPESTPTAKQIAREGGLRYVNDDTPGILRSRSDGSDGEFIYTDAHGKTIHDEATL 69 + P+ PE ++ ARE GLRYV+D+ PG R R+ F Y D G+ + DEA L Sbjct: 6 ETAPSAPEGRADPREAAREIGLRYVSDEEPGYRRKRNGRG---FRYIDPDGRPVRDEAVL 62 Query: 70 ARIKAMAIPPAWEEVWICPWANGHIQATGRDSRQRKQYRYHARWRIVRDEVKYQRMIAFA 129 RI+A+AIPPA+ +VWIC NGHIQATGRD R RKQYRYH ++R RD K+ M+ FA Sbjct: 63 KRIRALAIPPAYTDVWICRHPNGHIQATGRDDRGRKQYRYHPQFREARDSTKFAHMMDFA 122 Query: 130 QALPQIRKCLEHDLKKHGLNKQKVLATVVYLLQATLMRVGNDEYARNNQSFGITTLRNRH 189 +ALP +R ++ D+ + GL ++KVLATVV+LL+ TL+RVGND+YAR N+SFG+TTLR+ H Sbjct: 123 RALPALRARVQEDMGRRGLPREKVLATVVHLLETTLIRVGNDDYARANRSFGLTTLRDPH 182 Query: 190 VKVDGADIKFHFRGKSGVQHSIKLHDPYMVRIVRKLLDLPGQDLFQYLDEDGKTHTIGSG 249 V V+GA++KF F+GKSG + L D + +IV+ DLPGQ+LFQYLDEDG + S Sbjct: 183 VNVEGAELKFRFKGKSGKVWQLALRDRRVAKIVKACQDLPGQELFQYLDEDGVQRDVTSA 242 Query: 250 DVNDYLHEITGEDYTAKDFRTWAGTVLAALALDECRQFDSQAQAKKNIVQAIEHVAKKLG 309 DVN YL EITG D TAKDFRTW+GTVLAALAL E FDSQA AK+N+ AIE VA++LG Sbjct: 243 DVNAYLREITGRDITAKDFRTWSGTVLAALALREFETFDSQAAAKRNVRSAIERVAERLG 302 Query: 310 NTPTICRKCYVHPDVISSYLDGMTAGRLKEQVRDNLKQEMHALTPEEAAVLALL 363 NTPTICRKCY+HP+++ SYL+G R ++++ L++++H L PEE AVLALL Sbjct: 303 NTPTICRKCYIHPEILGSYLEGSFLLRARDEIEAELREDIHRLRPEETAVLALL 356 >ref|YP_002963036.1| DNA topoisomerase I [methylobacterium extorquens AM1] gb|ACS39759.1| putative DNA topoisomerase I [Methylobacterium extorquens AM1] Length = 400 Score = 414 bits (1065), Expect = e-114, Method: Compositional matrix adjust. Identities = 195/341 (57%), Positives = 256/341 (75%), Gaps = 3/341 (0%) Query: 23 KQIAREGGLRYVNDDTPGILRSRSDGSDGEFIYTDAHGKTIHDEATLARIKAMAIPPAWE 82 ++ AR+ GLRYV+D PG+ R R+ F Y D G + D +AR+K++AIPPA+ Sbjct: 14 REAARDAGLRYVDDSKPGLRRKRNGKG---FRYIDPKGAPVRDAEEIARLKSLAIPPAYT 70 Query: 83 EVWICPWANGHIQATGRDSRQRKQYRYHARWRIVRDEVKYQRMIAFAQALPQIRKCLEHD 142 EVWICP NGHIQATGRD + RKQYRYH R+R R+ K+ R++AFA+ALP IR ++ D Sbjct: 71 EVWICPHPNGHIQATGRDEKGRKQYRYHPRFREAREASKFHRIMAFAEALPGIRARIDAD 130 Query: 143 LKKHGLNKQKVLATVVYLLQATLMRVGNDEYARNNQSFGITTLRNRHVKVDGADIKFHFR 202 + K GL ++KVLATVV+LL+ TL+RVGND+YAR+N+S+G+TTLR+ HVKV G++++F F+ Sbjct: 131 MGKRGLPREKVLATVVHLLETTLIRVGNDDYARSNKSYGLTTLRDPHVKVAGSEMRFRFK 190 Query: 203 GKSGVQHSIKLHDPYMVRIVRKLLDLPGQDLFQYLDEDGKTHTIGSGDVNDYLHEITGED 262 GKSG + S+ + D + +IV+ DLPGQ+LFQYLDEDG+ + S DVN YL EITGED Sbjct: 191 GKSGKEWSVSVRDRRVAKIVKACQDLPGQELFQYLDEDGQRRDVTSSDVNAYLREITGED 250 Query: 263 YTAKDFRTWAGTVLAALALDECRQFDSQAQAKKNIVQAIEHVAKKLGNTPTICRKCYVHP 322 +TAKDFRTWAGTVLAALAL E FD+ A+AKKN+ AIE V+ +LGNTPTICRKCY+HP Sbjct: 251 FTAKDFRTWAGTVLAALALREFEAFDNAAKAKKNLRAAIESVSSRLGNTPTICRKCYIHP 310 Query: 323 DVISSYLDGMTAGRLKEQVRDNLKQEMHALTPEEAAVLALL 363 ++ YL+G ++KE V LK E+ L PEEAAVL+LL Sbjct: 311 QILDCYLEGGMLLQVKEAVEGELKNELDVLRPEEAAVLSLL 351 >ref|YP_002421026.1| DNA topoisomerase I [Methylobacterium chloromethanicum CM4] gb|ACK83098.1| putative DNA topoisomerase I [Methylobacterium chloromethanicum CM4] Length = 400 Score = 414 bits (1065), Expect = e-114, Method: Compositional matrix adjust. Identities = 195/341 (57%), Positives = 256/341 (75%), Gaps = 3/341 (0%) Query: 23 KQIAREGGLRYVNDDTPGILRSRSDGSDGEFIYTDAHGKTIHDEATLARIKAMAIPPAWE 82 ++ AR+ GLRYV+D PG+ R R+ F Y D G + D +AR+K++AIPPA+ Sbjct: 14 REAARDAGLRYVDDSKPGLRRKRNGKG---FRYIDPKGAPVRDAEEIARLKSLAIPPAYT 70 Query: 83 EVWICPWANGHIQATGRDSRQRKQYRYHARWRIVRDEVKYQRMIAFAQALPQIRKCLEHD 142 EVWICP NGHIQATGRD + RKQYRYH R+R R+ K+ R++AFA+ALP IR ++ D Sbjct: 71 EVWICPHPNGHIQATGRDEKGRKQYRYHPRFREAREASKFHRIMAFAEALPGIRARIDAD 130 Query: 143 LKKHGLNKQKVLATVVYLLQATLMRVGNDEYARNNQSFGITTLRNRHVKVDGADIKFHFR 202 + K GL ++KVLATVV+LL+ TL+RVGND+YAR+N+S+G+TTLR+ HVKV G++++F F+ Sbjct: 131 MGKRGLPREKVLATVVHLLETTLIRVGNDDYARSNKSYGLTTLRDPHVKVAGSEMRFRFK 190 Query: 203 GKSGVQHSIKLHDPYMVRIVRKLLDLPGQDLFQYLDEDGKTHTIGSGDVNDYLHEITGED 262 GKSG + S+ + D + +IV+ DLPGQ+LFQYLDEDG+ + S DVN YL EITGED Sbjct: 191 GKSGKEWSVSVRDRRVAKIVKACQDLPGQELFQYLDEDGQRRDVTSSDVNAYLREITGED 250 Query: 263 YTAKDFRTWAGTVLAALALDECRQFDSQAQAKKNIVQAIEHVAKKLGNTPTICRKCYVHP 322 +TAKDFRTWAGTVLAALAL E FD+ A+AKKN+ AIE V+ +LGNTPTICRKCY+HP Sbjct: 251 FTAKDFRTWAGTVLAALALREFEAFDNAAKAKKNLRAAIESVSSRLGNTPTICRKCYIHP 310 Query: 323 DVISSYLDGMTAGRLKEQVRDNLKQEMHALTPEEAAVLALL 363 ++ YL+G ++KE V LK E+ L PEEAAVL+LL Sbjct: 311 QILDCYLEGGMLLQVKEAVEGELKNELDVLRPEEAAVLSLL 351 >ref|YP_003068256.1| DNA topoisomerase I [Methylobacterium extorquens DM4] emb|CAX24396.1| putative DNA topoisomerase I [Methylobacterium extorquens DM4] Length = 400 Score = 413 bits (1061), Expect = e-113, Method: Compositional matrix adjust. Identities = 194/341 (56%), Positives = 256/341 (75%), Gaps = 3/341 (0%) Query: 23 KQIAREGGLRYVNDDTPGILRSRSDGSDGEFIYTDAHGKTIHDEATLARIKAMAIPPAWE 82 ++ AR+ GLRYV+D PG+ R R+ F Y D G + D +AR+K++AIPPA+ Sbjct: 14 REAARDAGLRYVDDSKPGLRRKRNGKG---FRYIDPKGAPVRDAEEIARLKSLAIPPAYT 70 Query: 83 EVWICPWANGHIQATGRDSRQRKQYRYHARWRIVRDEVKYQRMIAFAQALPQIRKCLEHD 142 EVWICP NGHIQATGRD + RKQYRYH R+R R+ K+ R++AFA+ALP IR ++ D Sbjct: 71 EVWICPHPNGHIQATGRDEKGRKQYRYHPRFREAREASKFHRIMAFAEALPGIRARIDAD 130 Query: 143 LKKHGLNKQKVLATVVYLLQATLMRVGNDEYARNNQSFGITTLRNRHVKVDGADIKFHFR 202 + K GL ++KVLATVV+LL+ TL+RVGND+YAR+N+S+G+TTLR+ HVKV G++++F F+ Sbjct: 131 MGKRGLPREKVLATVVHLLETTLIRVGNDDYARSNKSYGLTTLRDPHVKVAGSEMRFRFK 190 Query: 203 GKSGVQHSIKLHDPYMVRIVRKLLDLPGQDLFQYLDEDGKTHTIGSGDVNDYLHEITGED 262 GKSG + S+ + D + +IV+ DLPGQ+LFQYLDE+G+ + S DVN YL EITGED Sbjct: 191 GKSGKEWSVSVRDRRVAKIVKACQDLPGQELFQYLDEEGQRRDVTSSDVNAYLREITGED 250 Query: 263 YTAKDFRTWAGTVLAALALDECRQFDSQAQAKKNIVQAIEHVAKKLGNTPTICRKCYVHP 322 +TAKDFRTWAGTVLAALAL E FD+ A+AKKN+ AIE V+ +LGNTPTICRKCY+HP Sbjct: 251 FTAKDFRTWAGTVLAALALREFEAFDNAAKAKKNLRAAIESVSSRLGNTPTICRKCYIHP 310 Query: 323 DVISSYLDGMTAGRLKEQVRDNLKQEMHALTPEEAAVLALL 363 ++ YL+G ++KE V LK E+ L PEEAAVL+LL Sbjct: 311 QILDCYLEGGMLLQVKEAVEGELKNELDVLRPEEAAVLSLL 351