BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0070.p (366 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774121.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m... 669 0.0 ref|YP_004754284.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m... 652 0.0 ref|YP_001352063.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m... 648 0.0 ref|YP_001098679.1| tRNA-specific 2-thiouridylase MnmA [Herminii... 639 0.0 ref|ZP_08275115.1| tRNA (5-methylaminomethyl-2-thiouridylate)-me... 634 e-180 >ref|YP_003774121.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Herbaspirillum seropedicae SmR1] gb|ADJ62213.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase protein [Herbaspirillum seropedicae SmR1] Length = 368 Score = 669 bits (1726), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/365 (87%), Positives = 337/365 (92%), Gaps = 2/365 (0%) Query: 1 MAKKKVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAVSVA 60 M+KK+VVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTR+DWIDAVSVA Sbjct: 1 MSKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVA 60 Query: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMHLGADL 120 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAM LGADL Sbjct: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADL 120 Query: 121 IATGHYARVRENPVGKFELMKAVDASKDQSYFLHRLNQAQLSKTLFPLGEIHKTEVRRIA 180 IATGHYARVR+ P G F+L+KA+DASKDQSYFLHRLNQ QLS+T+FPLGEIHKTEVRRIA Sbjct: 121 IATGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRRIA 180 Query: 181 EQLKLPNATKKDSTGICFIGERPFREFLNRYLSYKPGPMKTPDGDIVGEHVGLSFYTLGQ 240 EQL+LPNA KKDSTGICFIGERPFREFLNRYLSYKPGP+KT DG VGEHVGLSFYTLGQ Sbjct: 181 EQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQ 240 Query: 241 RKGIGLGGIKAHQNADGNSDAWYVARKDVDTNTLYIVQGHEHPWLLSSQLGASQISWVAG 300 RKGIG+GG+KAHQNADG+ DAWYVARKDV+ NTLYIVQGH+HPWLLS L A Q+SWVAG Sbjct: 241 RKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWVAG 300 Query: 301 EAPNMSGAGLSAKTRYRQADVPCSFSDAGGRFDLRFSDPQWAVTPGQSAVLYQGDVCLGG 360 AP M A LSAKTRYRQADVPC FSDAG F L F PQWAVTPGQSAVLY GDVCLGG Sbjct: 301 HAPQM--AALSAKTRYRQADVPCGFSDAGASFSLAFDTPQWAVTPGQSAVLYDGDVCLGG 358 Query: 361 GIIES 365 GIIE+ Sbjct: 359 GIIEN 363 >ref|YP_004754284.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Collimonas fungivorans Ter331] gb|AEK63461.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Collimonas fungivorans Ter331] Length = 373 Score = 652 bits (1682), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/369 (84%), Positives = 338/369 (91%), Gaps = 8/369 (2%) Query: 2 AKKKVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAVSVAD 61 +KK+VVIGMSGGVDSSVSAW+LK+QGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAVSVAD Sbjct: 3 SKKRVVIGMSGGVDSSVSAWMLKQQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAVSVAD 62 Query: 62 VVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMHLGADLI 121 V+GVDIEAVNFA+EYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAM LGADLI Sbjct: 63 VIGVDIEAVNFAAEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADLI 122 Query: 122 ATGHYARVRENPVGKFELMKAVDASKDQSYFLHRLNQAQLSKTLFPLGEIHKTEVRRIAE 181 ATGHYARVR+ G+FEL+KAVDASKDQSYFLHRLNQ+QLS+TLFPLGEI KTEVR+IAE Sbjct: 123 ATGHYARVRQADSGRFELLKAVDASKDQSYFLHRLNQSQLSRTLFPLGEIQKTEVRKIAE 182 Query: 182 QLKLPNATKKDSTGICFIGERPFREFLNRYLSYKPGPMKTPDGDIVGEHVGLSFYTLGQR 241 Q++LPNA KKDSTGICFIGERPFREFLNRYLSYKPGPMKTPDGD+VGEHVGLSFYTLGQR Sbjct: 183 QIQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPMKTPDGDVVGEHVGLSFYTLGQR 242 Query: 242 KGIGLGGIKAHQNADGNSDAWYVARKDVDTNTLYIVQGHEHPWLLSSQLGASQISWVAGE 301 KGIGLGG+K H+NA G+SD WYVARKDV+ NTLYIVQGH+HPWLLSS L A Q+SWVAGE Sbjct: 243 KGIGLGGMKTHKNAGGDSDPWYVARKDVENNTLYIVQGHDHPWLLSSTLQAGQLSWVAGE 302 Query: 302 APNMSGAG-LSAKTRYRQADVPCSFSD----AGGRFDLRFSDPQWAVTPGQSAVLYQGDV 356 AP G+G LSAKTRYRQAD+ C+F F L FSDPQWAVTPGQSAVLY GD+ Sbjct: 303 AP---GSGQLSAKTRYRQADMACTFDSLRLPEAENFALSFSDPQWAVTPGQSAVLYDGDI 359 Query: 357 CLGGGIIES 365 CLGGGIIES Sbjct: 360 CLGGGIIES 368 >ref|YP_001352063.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Janthinobacterium sp. Marseille] sp|A6SUW6.1|MNMA_JANMA RecName: Full=tRNA-specific 2-thiouridylase mnmA gb|ABR89509.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Janthinobacterium sp. Marseille] Length = 372 Score = 648 bits (1672), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/370 (86%), Positives = 337/370 (91%), Gaps = 8/370 (2%) Query: 1 MAKKKVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAVSVA 60 MAKK+VVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDA SVA Sbjct: 1 MAKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAASVA 60 Query: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMHLGADL 120 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAM LGAD+ Sbjct: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMLLGADM 120 Query: 121 IATGHYARVRE----NPVGKFELMKAVDASKDQSYFLHRLNQAQLSKTLFPLGEIHKTEV 176 IATGHYARVRE G+ EL+KAVDASKDQSYFLHRLNQAQL+KTLFPLGEI KTEV Sbjct: 121 IATGHYARVREVTSGPDAGRVELLKAVDASKDQSYFLHRLNQAQLAKTLFPLGEIRKTEV 180 Query: 177 RRIAEQLKLPNATKKDSTGICFIGERPFREFLNRYLSYKPGPMKTPDGDIVGEHVGLSFY 236 R+IAEQLKLPNATKKDSTGICFIGERPFREFLNRYLSY+PGPMKTP+G IVGEHVGLSFY Sbjct: 181 RKIAEQLKLPNATKKDSTGICFIGERPFREFLNRYLSYQPGPMKTPEGVIVGEHVGLSFY 240 Query: 237 TLGQRKGIGLGGIKAHQNADGNSDAWYVARKDVDTNTLYIVQGHEHPWLLSSQLGASQIS 296 TLGQRKGIGLGG+K+H+N DGNS+ WYVARKDV TNTLYIVQGH+HPWLLSS+L A Q+S Sbjct: 241 TLGQRKGIGLGGMKSHKNTDGNSEPWYVARKDVATNTLYIVQGHDHPWLLSSELSAGQMS 300 Query: 297 WVAGEAPNMSGAGLSAKTRYRQADVPCS-FSDAGGRFDLRFSDPQWAVTPGQSAVLYQGD 355 WVAG P S +SAKTRYRQADV CS SDA G F L F+ PQWAVTPGQSAVLYQGD Sbjct: 301 WVAGSPP--SEELVSAKTRYRQADVACSQRSDADG-FSLAFAAPQWAVTPGQSAVLYQGD 357 Query: 356 VCLGGGIIES 365 VCLGGGII + Sbjct: 358 VCLGGGIINT 367 >ref|YP_001098679.1| tRNA-specific 2-thiouridylase MnmA [Herminiimonas arsenicoxydans] sp|A4G213.1|MNMA_HERAR RecName: Full=tRNA-specific 2-thiouridylase mnmA emb|CAL60550.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Herminiimonas arsenicoxydans] Length = 372 Score = 639 bits (1649), Expect = 0.0, Method: Compositional matrix adjust. Identities = 310/369 (84%), Positives = 328/369 (88%), Gaps = 6/369 (1%) Query: 1 MAKKKVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAVSVA 60 MAKK+VVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDA SVA Sbjct: 1 MAKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAASVA 60 Query: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMHLGADL 120 DVVGVDIEAVNFA+EYKDRVFAEFLREY+AGRTPNPDVLCNAEIKFKAFLDHAM LGAD+ Sbjct: 61 DVVGVDIEAVNFAAEYKDRVFAEFLREYEAGRTPNPDVLCNAEIKFKAFLDHAMLLGADM 120 Query: 121 IATGHYARVRENP----VGKFELMKAVDASKDQSYFLHRLNQAQLSKTLFPLGEIHKTEV 176 IATGHYARVRE G+ +L+KAVDASKDQSYFLHRLNQAQLSKTLFPLGEI KTEV Sbjct: 121 IATGHYARVREVTSGPDAGQVQLLKAVDASKDQSYFLHRLNQAQLSKTLFPLGEIRKTEV 180 Query: 177 RRIAEQLKLPNATKKDSTGICFIGERPFREFLNRYLSYKPGPMKTPDGDIVGEHVGLSFY 236 R+IAEQL+LPNA+KKDSTGICFIGERPFREFLNRYLSY+PGPMKTPDG IVGEHVGLSFY Sbjct: 181 RKIAEQLQLPNASKKDSTGICFIGERPFREFLNRYLSYQPGPMKTPDGTIVGEHVGLSFY 240 Query: 237 TLGQRKGIGLGGIKAHQNADGNSDAWYVARKDVDTNTLYIVQGHEHPWLLSSQLGASQIS 296 TLGQRKGIGLGG+K H+N DGNS WYVARKDV NTLYIVQGH+HPWLLSS L A Q+S Sbjct: 241 TLGQRKGIGLGGMKTHKNTDGNSGPWYVARKDVANNTLYIVQGHDHPWLLSSALSAGQMS 300 Query: 297 WVAGEAPNMSGAGLSAKTRYRQADVPCSFSDAGGRFDLRFSDPQWAVTPGQSAVLYQGDV 356 WVAG AP S ++AKTRYRQADV CS L F PQWAVTPGQSAVLYQGDV Sbjct: 301 WVAGRAP--SEELVAAKTRYRQADVACSQHATADNLALAFDTPQWAVTPGQSAVLYQGDV 358 Query: 357 CLGGGIIES 365 CLGGGII + Sbjct: 359 CLGGGIITA 367 >ref|ZP_08275115.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Oxalobacteraceae bacterium IMCC9480] gb|EGF31411.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Oxalobacteraceae bacterium IMCC9480] Length = 369 Score = 634 bits (1635), Expect = e-180, Method: Compositional matrix adjust. Identities = 303/368 (82%), Positives = 332/368 (90%), Gaps = 5/368 (1%) Query: 1 MAKKKVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAVSVA 60 M+KKKVVIG+SGGVDSSVSA LLKEQGYEVIGLFMKNWEDDDD+EYCSTRQDWIDAVSVA Sbjct: 1 MSKKKVVIGLSGGVDSSVSAALLKEQGYEVIGLFMKNWEDDDDAEYCSTRQDWIDAVSVA 60 Query: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMHLGADL 120 DV+G+DIEAVNF++EYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMHLGADL Sbjct: 61 DVLGIDIEAVNFSAEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMHLGADL 120 Query: 121 IATGHYARVRENP--VGKFELMKAVDASKDQSYFLHRLNQAQLSKTLFPLGEIHKTEVRR 178 IATGHYARVRE+ G+FEL+KA+D +KDQSYFLHRLNQAQLSKTLFPLGEI KTEVR+ Sbjct: 121 IATGHYARVRESASDAGRFELLKAIDHTKDQSYFLHRLNQAQLSKTLFPLGEIRKTEVRK 180 Query: 179 IAEQLKLPNATKKDSTGICFIGERPFREFLNRYLSYKPGPMKTPDGDIVGEHVGLSFYTL 238 +AEQL LPNA KKDSTGICFIGERPFREFLNRYLSY+PGPMKTP+GD +GEHVGLSFYTL Sbjct: 181 MAEQLGLPNAAKKDSTGICFIGERPFREFLNRYLSYQPGPMKTPEGDTIGEHVGLSFYTL 240 Query: 239 GQRKGIGLGGIKAHQNADGNSDAWYVARKDVDTNTLYIVQGHEHPWLLSSQLGASQISWV 298 GQRKGIG+GGIKAHQNA+G+SDAWYVARKD+ +NTLY+VQGH+HPWLLS+ L A Q+SW+ Sbjct: 241 GQRKGIGIGGIKAHQNANGSSDAWYVARKDIASNTLYVVQGHDHPWLLSASLEADQLSWI 300 Query: 299 AGEAPNMSGAGLSAKTRYRQADVPCSFSDAGG-RFDLRFSDPQWAVTPGQSAVLYQGDVC 357 AG P+ LSAKTRYRQADVPC R L F QWAVTPGQSAVLY+GDVC Sbjct: 301 AGAPPD--DGQLSAKTRYRQADVPCHVGMVDAQRLQLAFDASQWAVTPGQSAVLYRGDVC 358 Query: 358 LGGGIIES 365 LGGGII S Sbjct: 359 LGGGIIAS 366