BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0118.p (372 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776585.1| fatty acid desaturase [Herbaspirillum serope... 589 e-166 ref|YP_001022784.1| putative fatty acid desaturase [Methylibium ... 491 e-137 ref|YP_296636.1| Fatty acid desaturase [Ralstonia eutropha JMP13... 488 e-135 ref|YP_004684793.1| fatty acid desaturase [Cupriavidus necator N... 486 e-135 ref|YP_725522.1| fatty acid desaturase [Ralstonia eutropha H16] ... 485 e-135 >ref|YP_003776585.1| fatty acid desaturase [Herbaspirillum seropedicae SmR1] gb|ADJ64677.1| fatty acid desaturase protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 589 bits (1518), Expect = e-166, Method: Compositional matrix adjust. Identities = 277/351 (78%), Positives = 309/351 (88%) Query: 10 PVDPHAPMPHRKVIRSWLAPVVARSTPLAIALVVLDFCLFGLALAATIRVDNILAKVALG 69 PV P APMPHRKVIRSW+ P+ RST +A+ L V+DF LFG +LAA + +LAK+ALG Sbjct: 28 PVAPGAPMPHRKVIRSWVIPLSKRSTAIALLLSVIDFALFGASLAAIVWAPWLLAKIALG 87 Query: 70 MLTGFIIGRLFILGHDACHQSFTANRRLNTWLGRLVFMPSLTPYSLWAVGHNVVHHGYTN 129 ++ GFIIGRLFILGHDACHQ+FT NR+LNTWLGRL+FMPSLTPYSLWAVGHNVVHHGYTN Sbjct: 88 VVNGFIIGRLFILGHDACHQAFTPNRKLNTWLGRLLFMPSLTPYSLWAVGHNVVHHGYTN 147 Query: 130 LKGFDFVWQPHSLQEFRALPRWRQKLERLYRSGWAPWLYYTVDMWWKKMYFPSRKQMPTR 189 LKGFDFVWQP SL+EF ALPRWRQ+LER+YRSG+ P LYY +DMWWK+MYFPS+KQMPTR Sbjct: 148 LKGFDFVWQPRSLEEFNALPRWRQRLERIYRSGFGPGLYYMIDMWWKRMYFPSKKQMPTR 207 Query: 190 RAEFIWDGVLVSVAATVWIGGLAWAAQSGGQSFLTLLLTAFVVPVLFWKAMIGFVVYVHH 249 RAEF+WDG LV+ A VW+GGLAWAAQS GQSF TLL+T FV+P+LFWKAMIGFVVYVHH Sbjct: 208 RAEFMWDGALVTAVAAVWVGGLAWAAQSTGQSFWTLLVTGFVIPLLFWKAMIGFVVYVHH 267 Query: 250 THVGVAWYQDKSEWSAAQPFVSTTVHLTFRHGIGAALHHIMEHTAHHVDMSVPLYRLKKA 309 TH VAWY DK W+AAQPFVSTTVHLTF GA LHHIMEHTAHHVDMSVPLYRLK+A Sbjct: 268 THTSVAWYDDKVTWAAAQPFVSTTVHLTFGRFWGALLHHIMEHTAHHVDMSVPLYRLKQA 327 Query: 310 QKLLETMLPGRIVIQQFSWRWYFDTARRCKLYDFDRLCWTDFKGRPTSTPL 360 QK LETMLPGRIVIQ+FSWRWYFDTARRCKLYDF RLCWTD++GRPTS PL Sbjct: 328 QKKLETMLPGRIVIQRFSWRWYFDTARRCKLYDFKRLCWTDYRGRPTSQPL 378 >ref|YP_001022784.1| putative fatty acid desaturase [Methylibium petroleiphilum PM1] gb|ABM96549.1| putative fatty acid desaturase [Methylibium petroleiphilum PM1] Length = 364 Score = 491 bits (1265), Expect = e-137, Method: Compositional matrix adjust. Identities = 246/359 (68%), Positives = 286/359 (79%), Gaps = 1/359 (0%) Query: 4 PDSTLTPVDPHAPMPHRKVIRSWLAPVVARSTPLAIALVVLDFCLFGLALAATIRVDNIL 63 PD P A +PHRK+IR+WLAP+ +RST A+AL ++D L G AL AT+ + + Sbjct: 6 PDLAPPSAAPSA-IPHRKLIRAWLAPLASRSTAYALALTLVDLLLLGAALTATVAFETLP 64 Query: 64 AKVALGMLTGFIIGRLFILGHDACHQSFTANRRLNTWLGRLVFMPSLTPYSLWAVGHNVV 123 K+ GM GF+IGRLFILGHDACHQS T +RRLN WLGRLVF+PSLTPYSLW VGHNVV Sbjct: 65 MKLLAGMAAGFVIGRLFILGHDACHQSLTPHRRLNRWLGRLVFLPSLTPYSLWEVGHNVV 124 Query: 124 HHGYTNLKGFDFVWQPHSLQEFRALPRWRQKLERLYRSGWAPWLYYTVDMWWKKMYFPSR 183 HHGYTNLKGFDFVW+P +L+ +RALPRWR++LER+YRSGWAPWLYY V++WW ++ FP+R Sbjct: 125 HHGYTNLKGFDFVWEPRTLEAYRALPRWRRRLERVYRSGWAPWLYYLVEIWWNRLLFPNR 184 Query: 184 KQMPTRRAEFIWDGVLVSVAATVWIGGLAWAAQSGGQSFLTLLLTAFVVPVLFWKAMIGF 243 + MPTRRA F WDGVLV A WI GL AA + GQS L+LT FV+P +FW MIGF Sbjct: 185 RTMPTRRAAFFWDGVLVVAGAAAWIAGLVAAAAATGQSATFLVLTGFVLPFVFWNGMIGF 244 Query: 244 VVYVHHTHVGVAWYQDKSEWSAAQPFVSTTVHLTFRHGIGAALHHIMEHTAHHVDMSVPL 303 VVYVHHTH GVAWY+DK W+ AQPFVSTTVHLTFR +G+ALHHIMEHTAHHVDMSVPL Sbjct: 245 VVYVHHTHTGVAWYEDKRRWADAQPFVSTTVHLTFRSFMGSALHHIMEHTAHHVDMSVPL 304 Query: 304 YRLKKAQKLLETMLPGRIVIQQFSWRWYFDTARRCKLYDFDRLCWTDFKGRPTSTPLRA 362 YRLK+AQ+LLE L G IV+Q FSWRWY DTARRCKLYDF+ CWTDF GR TSTPL A Sbjct: 305 YRLKRAQRLLEERLAGHIVVQPFSWRWYADTARRCKLYDFEAGCWTDFSGRRTSTPLAA 363 >ref|YP_296636.1| Fatty acid desaturase [Ralstonia eutropha JMP134] gb|AAZ61792.1| Fatty acid desaturase [Ralstonia eutropha JMP134] Length = 380 Score = 488 bits (1255), Expect = e-135, Method: Compositional matrix adjust. Identities = 228/348 (65%), Positives = 268/348 (77%) Query: 10 PVDPHAPMPHRKVIRSWLAPVVARSTPLAIALVVLDFCLFGLALAATIRVDNILAKVALG 69 P+ P P+P RK+IR WL P+ RSTP A+AL D+ LF LA + + AKV LG Sbjct: 26 PISPDQPLPSRKIIRGWLIPLGQRSTPRALALFAFDYLLFAATLAGVVLAPHWAAKVGLG 85 Query: 70 MLTGFIIGRLFILGHDACHQSFTANRRLNTWLGRLVFMPSLTPYSLWAVGHNVVHHGYTN 129 ++ G II RLFI+GHDACHQS T R LN WLGRL F+PSLTPYSLW VGHNVVHHGYTN Sbjct: 86 VVAGLIIARLFIIGHDACHQSLTPRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYTN 145 Query: 130 LKGFDFVWQPHSLQEFRALPRWRQKLERLYRSGWAPWLYYTVDMWWKKMYFPSRKQMPTR 189 LKGFDFVW P+SL+EF+ALPRWR+ +ER+YR+G+ P LYY V++WW K++FP+++QM TR Sbjct: 146 LKGFDFVWAPYSLEEFQALPRWRRVMERIYRTGFGPGLYYLVEIWWCKLFFPNKRQMATR 205 Query: 190 RAEFIWDGVLVSVAATVWIGGLAWAAQSGGQSFLTLLLTAFVVPVLFWKAMIGFVVYVHH 249 R F+ D LV+ WIG L WAA + GQS ++ FV+P L W +GFV+YVHH Sbjct: 206 RPIFMADCTLVATFGAAWIGALIWAAFATGQSAWMMVGAGFVLPFLVWNVTVGFVLYVHH 265 Query: 250 THVGVAWYQDKSEWSAAQPFVSTTVHLTFRHGIGAALHHIMEHTAHHVDMSVPLYRLKKA 309 TH VAWY KS W+ AQPFVSTTVHL FRHGIGAALHHIMEHTAHHVDMSVPLYRLK+A Sbjct: 266 THTSVAWYDTKSMWAKAQPFVSTTVHLRFRHGIGAALHHIMEHTAHHVDMSVPLYRLKRA 325 Query: 310 QKLLETMLPGRIVIQQFSWRWYFDTARRCKLYDFDRLCWTDFKGRPTS 357 Q LLE LPGRI+I+ FSWRWYFDTARRCKLYDF LCWTDF+GR TS Sbjct: 326 QALLEHALPGRIIIENFSWRWYFDTARRCKLYDFKALCWTDFRGRQTS 373 >ref|YP_004684793.1| fatty acid desaturase [Cupriavidus necator N-1] gb|AEI76312.1| fatty acid desaturase [Cupriavidus necator N-1] Length = 391 Score = 486 bits (1252), Expect = e-135, Method: Compositional matrix adjust. Identities = 231/349 (66%), Positives = 267/349 (76%) Query: 9 TPVDPHAPMPHRKVIRSWLAPVVARSTPLAIALVVLDFCLFGLALAATIRVDNILAKVAL 68 P+ P AP+P RK+IR WL P+ RSTP A+ L D+ LFG LA + + AK+AL Sbjct: 36 APIAPDAPLPSRKIIRGWLIPLGQRSTPRALLLFAFDYLLFGAVLAGVVLAQHWAAKLAL 95 Query: 69 GMLTGFIIGRLFILGHDACHQSFTANRRLNTWLGRLVFMPSLTPYSLWAVGHNVVHHGYT 128 G+L G II RLFI+GHDACHQS T R LN WLGRL F+PSLTPYSLW VGHNVVHHGYT Sbjct: 96 GVLAGLIIARLFIIGHDACHQSLTPRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYT 155 Query: 129 NLKGFDFVWQPHSLQEFRALPRWRQKLERLYRSGWAPWLYYTVDMWWKKMYFPSRKQMPT 188 NLKGFDFVW P+SL+EF ALPRWR+ +ER+YR+G+ P LYY V++WW KM+FPS++QM T Sbjct: 156 NLKGFDFVWAPYSLEEFNALPRWRRVMERIYRTGFGPGLYYLVEIWWCKMFFPSKRQMAT 215 Query: 189 RRAEFIWDGVLVSVAATVWIGGLAWAAQSGGQSFLTLLLTAFVVPVLFWKAMIGFVVYVH 248 RR F D VLV+ WIG LA A + QS L+ FV+P L W +GFV+YVH Sbjct: 216 RRPIFTGDCVLVAAFGLAWIGALAGLALATDQSVWMLIGAGFVLPFLVWNVTVGFVLYVH 275 Query: 249 HTHVGVAWYQDKSEWSAAQPFVSTTVHLTFRHGIGAALHHIMEHTAHHVDMSVPLYRLKK 308 HTH VAWY K+ W+ AQPFVSTTVHL FRHGIGAALHHIMEHTAHHVDMSVPLYRLK+ Sbjct: 276 HTHTSVAWYDTKAMWAKAQPFVSTTVHLRFRHGIGAALHHIMEHTAHHVDMSVPLYRLKR 335 Query: 309 AQKLLETMLPGRIVIQQFSWRWYFDTARRCKLYDFDRLCWTDFKGRPTS 357 AQ LLE LPGRI+I+ FSWRWYFDTARRCKLYDF LCWTDF+GR TS Sbjct: 336 AQALLEHALPGRIIIENFSWRWYFDTARRCKLYDFKALCWTDFRGRQTS 384 >ref|YP_725522.1| fatty acid desaturase [Ralstonia eutropha H16] emb|CAJ92154.1| fatty acid desaturase [Ralstonia eutropha H16] Length = 391 Score = 485 bits (1249), Expect = e-135, Method: Compositional matrix adjust. Identities = 231/348 (66%), Positives = 266/348 (76%) Query: 10 PVDPHAPMPHRKVIRSWLAPVVARSTPLAIALVVLDFCLFGLALAATIRVDNILAKVALG 69 P+ P AP+P RK+IR WL P+ RSTP A+ L D+ LFG LA + + AK+ALG Sbjct: 37 PIAPDAPLPSRKIIRGWLIPLGQRSTPRALLLFAFDYLLFGAVLAGVVLAQHWAAKLALG 96 Query: 70 MLTGFIIGRLFILGHDACHQSFTANRRLNTWLGRLVFMPSLTPYSLWAVGHNVVHHGYTN 129 +L G II RLFI+GHDACHQS T R LN WLGRL F+PSLTPYSLW VGHNVVHHGYTN Sbjct: 97 VLAGLIIARLFIIGHDACHQSLTPRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYTN 156 Query: 130 LKGFDFVWQPHSLQEFRALPRWRQKLERLYRSGWAPWLYYTVDMWWKKMYFPSRKQMPTR 189 LKGFDFVW P+SL+EF ALPRWR+ +ER+YR+G+ P LYY V++WW KM+FPS++QM TR Sbjct: 157 LKGFDFVWAPYSLEEFNALPRWRRVMERIYRTGFGPGLYYLVEIWWCKMFFPSKRQMATR 216 Query: 190 RAEFIWDGVLVSVAATVWIGGLAWAAQSGGQSFLTLLLTAFVVPVLFWKAMIGFVVYVHH 249 R F D VLV+ WIG LA A + QS L FV+P L W +GFV+YVHH Sbjct: 217 RPIFTGDCVLVAAFGLAWIGALAGLALATEQSVWLLTGAGFVLPFLVWNVTVGFVLYVHH 276 Query: 250 THVGVAWYQDKSEWSAAQPFVSTTVHLTFRHGIGAALHHIMEHTAHHVDMSVPLYRLKKA 309 TH VAWY K+ W+ AQPFVSTTVHL FRHGIGAALHHIMEHTAHHVDMSVPLYRLK+A Sbjct: 277 THTSVAWYDTKAMWAKAQPFVSTTVHLRFRHGIGAALHHIMEHTAHHVDMSVPLYRLKRA 336 Query: 310 QKLLETMLPGRIVIQQFSWRWYFDTARRCKLYDFDRLCWTDFKGRPTS 357 Q LLE LPGRI+I+ FSWRWYFDTARRCKLYDF LCWTDF+GR TS Sbjct: 337 QALLEHALPGRIIIENFSWRWYFDTARRCKLYDFKALCWTDFRGRQTS 384