BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0038.p (1119 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775732.1| alpha-amylase [Herbaspirillum seropedicae Sm... 1451 0.0 ref|ZP_07673847.1| alpha-amylase family protein [Ralstonia sp. 5... 975 0.0 ref|NP_521802.1| putative alpha-amylase-related protein [Ralston... 972 0.0 ref|YP_001892471.1| alpha amylase catalytic region [Ralstonia pi... 960 0.0 ref|YP_001860554.1| alpha amylase catalytic region [Burkholderia... 958 0.0 >ref|YP_003775732.1| alpha-amylase [Herbaspirillum seropedicae SmR1] gb|ADJ63824.1| alpha-amylase protein [Herbaspirillum seropedicae SmR1] Length = 1132 Score = 1451 bits (3757), Expect = 0.0, Method: Compositional matrix adjust. Identities = 738/1161 (63%), Positives = 863/1161 (74%), Gaps = 71/1161 (6%) Query: 1 LENHAAAAFSPRIYCIDPTLVGGGGWSDLFARCAGMGFDHVLLCGDGLIAGLFAGEDSHV 60 +EN FSPRIYC+DP+LV W DL RCA MGFDHVLLCGD L+ FA Sbjct: 1 MENLPLTDFSPRIYCVDPSLVASPDWDDLLGRCAAMGFDHVLLCGD-LVDASFA------ 53 Query: 61 DNVESPIADYGMASLDQLEELAHLATEHRLRLLLDLSLTSVHGSDQLLRSHPDWFAVAGA 120 E+A L HRLRLL DLSLT + D +L SH DWFA + Sbjct: 54 -------------------EVAKLCKGHRLRLLADLSLTRFYAGDPVLTSHADWFAPQSS 94 Query: 121 A----SDASAHGDHLPDPRRP-------------QPRFHAPEIAEALLECWHMRLAMLID 163 A S D LPDPR P + RF P A+A+L+ W RL L+D Sbjct: 95 AAVGLSSQDLASDPLPDPRYPSVMDERDDELVTRRARFDDPVAADAMLDWWRARLMTLVD 154 Query: 164 AGVAGFLCHDPAGLPGNFWCRLVERTREQRSCCQFLAWTPGCSHPQLESLADCGFDAVFS 223 AGVAGF C +PA +P +FW RL+ R+Q++ C+FLAWTPGC+H QL+ LA GFDAVFS Sbjct: 155 AGVAGFRCQEPASIPADFWRRLIAGIRQQQADCRFLAWTPGCTHDQLDGLAGIGFDAVFS 214 Query: 224 SGAWWDFRAPWYFREHEKLVAIAPVLGFPEDPFGPRAARVHGWHDKETRRLGAIRALRFA 283 SGAWWDFR PWYFRE+ KL AIAPVL FPEDP GPR ARVHG+ D +TRRLGA+RAL+FA Sbjct: 215 SGAWWDFRDPWYFREYRKLAAIAPVLAFPEDPSGPRLARVHGFRDPQTRRLGALRALQFA 274 Query: 284 ALSGSGWLMPMGFEFGLTEALGIAHNAAASTRLTTPQQFAQACAESDFNLDAEVRAANQA 343 LS GW+MPMGFEFGLT AL +AH A ++TRL+ P+ F +ACA++D + E+ AN+ Sbjct: 275 TLSAEGWMMPMGFEFGLTAALRVAHRAGSATRLSDPEAFKRACADADLDFTREITEANRT 334 Query: 344 MS--------AGGFRLRAVSAPEAPVLLLAR---DVGVHQLIAINPRLGQVARLADGDWQ 392 ++ + RL +SAP++P LL+ R +V LIA+NP L + R+ D WQ Sbjct: 335 LAQRRQSPPKSLALRLTPLSAPDSPWLLMERHGVNVEDGLLIAVNPALDRPVRITDDGWQ 394 Query: 393 QRLDGRIVDWPPEREFILQPGEIALIELQTPPPVAADLSRRKKPVAEKVRTGRIAIEQVS 452 QRL + W E +L+PG + ++ L+ V+ +L + P +R+ RIAIE+VS Sbjct: 395 QRLQESVAPWQ-EDGLVLEPGAVKVLPLKPLQGVSGELDQ-ATPALGDMRSRRIAIERVS 452 Query: 453 PAVDGGRFAVKRTVGQSFTISADIFMDGHDHLAAAVLVRAADEKQWQRIPLQHVGNDRWQ 512 PAVD G FA KR VG+ F I+ADIFMDGHDHLAA VLVRAADE QW+RIP+ NDRWQ Sbjct: 453 PAVDDGAFAAKRIVGEHFVIAADIFMDGHDHLAAEVLVRAADEAQWRRIPMHPDVNDRWQ 512 Query: 513 ASLSLPRLGRHYFCIEAWSDVFETYRDGLKKKSAAGLNVDLELEEGRLLVANLAEQALEN 572 A +SL RLGRHYFCIEAWSDVF+T+RDGL+KK AG +V LE+EEGRLL+A L ++A+E Sbjct: 513 ARVSLTRLGRHYFCIEAWSDVFDTFRDGLQKKLMAGQDVSLEMEEGRLLIARLVQRAVEQ 572 Query: 573 GIDGAVLEGGGDLLKSL-----------SGKNGNHNGNRLAVLLADDTARQIDRLTELAG 621 ++ +VLE LLK+L +GK + + RLA L A++T + +++LT LAG Sbjct: 573 ELEPSVLESSRALLKALGGPPSKTRRSAAGKQADTD-KRLAFLCAEETRQLVEQLTGLAG 631 Query: 622 ARAFLMRSATEYPLETERPAATFSSWYELFPRSQSGDAAVHGTFNDVIARLPAIRAMGFD 681 RAFL R+ EYPLE ER +A FSSWYELFPRSQSG A VHG F+DVIARLPAIRAMGFD Sbjct: 632 ERAFLSRTDAEYPLEAERRSAAFSSWYELFPRSQSGSADVHGNFDDVIARLPAIRAMGFD 691 Query: 682 TLYFPPIHPIGKKNRKGKNNSLTPGDDDPGSPYAIGSEEGGHDAIHPQLGTLDDFLRLRD 741 TLYFPPIHPIGKK+RKGKNNS+T G DDPGSPYAIGSEEGGHDAIHPQLGT +DF RLRD Sbjct: 692 TLYFPPIHPIGKKHRKGKNNSVTAGPDDPGSPYAIGSEEGGHDAIHPQLGTFEDFARLRD 751 Query: 742 AAAAHGLELALDFAIQCAPDHPWLKEHPGWFSWRPDGSMRYAENPPKKYQDIVNVDFYAE 801 AAAAHGLELALDFAIQC+PDHPWLK HPGWF+WRPDGSMRYAENPPKKYQDIVNVDFYAE Sbjct: 752 AAAAHGLELALDFAIQCSPDHPWLKTHPGWFAWRPDGSMRYAENPPKKYQDIVNVDFYAE 811 Query: 802 DAIPDLWIALRDIVLYWVQQGVNVFRVDNPHTKPFPFWEWMIAAVRSRYPQVIFLSEAFT 861 DAIP+LW+ALRDIV++WVQQGV+VFRVDNPHTKPFPFWEWMIA+VRSRYPQVIFLSEAFT Sbjct: 812 DAIPELWLALRDIVVFWVQQGVSVFRVDNPHTKPFPFWEWMIASVRSRYPQVIFLSEAFT 871 Query: 862 RPKPMYRLAKVGFSQSYTYFTWRHDKQEFIDYLEELTGSPDGDTRPRDFFRPHFFVNTPD 921 RPKPMYRLAKVGFSQSYTYFTWRH KQE IDYL ELT +PRDFFRPHFFVNTPD Sbjct: 872 RPKPMYRLAKVGFSQSYTYFTWRHGKQELIDYLTELTTDTGHAAQPRDFFRPHFFVNTPD 931 Query: 922 INPYFLQRSGRAGFVIRAALAATLSGLWGVYSGFELCEAQPVPGKEEYLDSEKYQVRAWD 981 INPYFLQRSGRAGF IRAALAATLSGLWGVYSGFEL EA VPGKEEYLDSEKY++R+WD Sbjct: 932 INPYFLQRSGRAGFQIRAALAATLSGLWGVYSGFELGEANAVPGKEEYLDSEKYEIRSWD 991 Query: 982 WQRPGNIIEDIARLNRIRRENPALQTHLGVHFLPSSNDKVLYFVKSTAPAYSVVDAVDDR 1041 WQRPGNIIEDI RLNRIR ENPALQTHLGV FLP SND++LYFVKST A S AV+ Sbjct: 992 WQRPGNIIEDITRLNRIRTENPALQTHLGVRFLPVSNDQILYFVKSTPAAPSGRAAVEGG 1051 Query: 1042 LRFGDNVILVAINLDPFNEQSGDIDIPLYGFGL---GDDSRVQVEDLLTDRRFNWTGRRH 1098 LRFGDN ILVAINLDPF Q G+I++PL GFGL + + ++V DLL + RF W GR Sbjct: 1052 LRFGDNTILVAINLDPFQSQQGEIELPLAGFGLPEGAEPAHLEVVDLLGEDRFKWYGRHQ 1111 Query: 1099 QLSLNPDKNPYGIWRLRAMET 1119 ++L+P + PY IWR+RA ET Sbjct: 1112 SITLDPRERPYRIWRIRAGET 1132 >ref|ZP_07673847.1| alpha-amylase family protein [Ralstonia sp. 5_7_47FAA] gb|EFP67775.1| alpha-amylase family protein [Ralstonia sp. 5_7_47FAA] Length = 1199 Score = 975 bits (2520), Expect = 0.0, Method: Compositional matrix adjust. Identities = 560/1174 (47%), Positives = 697/1174 (59%), Gaps = 100/1174 (8%) Query: 8 AFSPRIYCIDPTLVGG-GGWSDLFARCAGMGFDHVLLC---GDGLIAGLFAGEDSHVDNV 63 A++P I + P +G W + R A +GFDHVL+ G +F D + Sbjct: 50 AYAPAICYVHPLQLGRLDRWDGVLDRIAALGFDHVLMAPPFAVGAAGNVFVTRD----HA 105 Query: 64 ESPIADYGMASLDQLEELAHLATEHRLRLLLDLSLTSVHGSDQLLRSHPDWFAVAGAASD 123 + A G + L LA L LLLDL + V L HPDWF D Sbjct: 106 QLHAALGGGPADAALATLAEGCRSRGLTLLLDLVIDRVAIDAPLRSDHPDWFISHAEDGD 165 Query: 124 ASAHGDHLPDPRRPQP-------RFHAPEIAEALLECWHMRLAMLIDAGVAGFLCHDPAG 176 A DPR P R P A+A+L W +L G+ GF C PA Sbjct: 166 AL-------DPRLPPSHLEVASFRHDDPRTADAILTWWTQQLRSWAALGIGGFRCEAPAR 218 Query: 177 LPGNFWCRLVERTREQRSCCQFLAWTPGCSHPQLESLADCGFDAVFSSGAWWDFRAPWYF 236 LP W L+ R++ + +FLAWTPG +++LA GFDA FSS WWDFRA W Sbjct: 219 LPAERWRTLIADVRKEAAAIRFLAWTPGMDGAAVDALAGAGFDATFSSLRWWDFRAGWMV 278 Query: 237 REHEKLVAIAPVLGFPEDPFGPRAARVHGWHDKETRRLGAIRALRFAALSGSGWLMPMGF 296 EH +L A+AP + E PFG R + G D R R L A +GWLMP+GF Sbjct: 279 EEHARLAAVAPPIATVEAPFGTRYGQAFG--DAMIRERAYRRLLHVADTLDAGWLMPLGF 336 Query: 297 EFGLTEALGIAHNAAASTRLTTPQQFAQACAESDFNLDAEVRAANQAMS-AGGFRLRAVS 355 E G + +A S + A +++ +++A +RAA + + A LR ++ Sbjct: 337 ERGALLPM-LAERGDPSDQQWIDAHAAFDLSDAVRDVNATIRAARETNTVAPHAELRMLT 395 Query: 356 APEAPVLLLARDVG-------VHQLIAINPRLGQ-VARLAD-------GDWQQRLDGRIV 400 P+AP L R G L INP L V+ AD G + + + ++ Sbjct: 396 GPDAPATALLRADGPDLRAGDAATLTVINPDLYMGVSVHADHFLPGVAGGFTRGVALDLL 455 Query: 401 DWP-------------PEREFILQPGEIALIEL---------------QTPPPVAADLSR 432 P P E L PG++ + + ++P A Sbjct: 456 TEPVGSCDDTLRANARPLAEIDLLPGDVQVWRVFRNAPVRAPAAARPAKSP---RAKAGE 512 Query: 433 RKKPVAEKVRTGRIAIEQVSPAVDGGRFAVKRTVGQSFTISADIFMDGHDHLAAAVLVRA 492 K PV + + RI IEQV P+V+ GRFAVKR VG + AD+ MDGHD LA +L RA Sbjct: 513 AKDPVNAAIASPRIGIEQVQPSVEDGRFAVKRLVGDDIVVEADVLMDGHDKLAVQLLWRA 572 Query: 493 ADEKQWQRIPLQHVGNDRWQASLSLPRLGRHYFCIEAWSDVFETYRDGLKKKSAAGLNVD 552 DE WQ +P+ +GNDRW+ + RLGRH + + AW D F TYR L+KK AG++V Sbjct: 573 QDEDSWQHVPMVALGNDRWRGAFRPERLGRHVYAVAAWRDAFGTYRSELEKKHTAGVDVT 632 Query: 553 LELEEGRLLVANLAEQALENGIDGAVLEGGGDLLKSLSGKNGNHNGNRLAVLLADDTARQ 612 LELEEG LVA AE A +N A+ + L + LS + H RL +LL TA Sbjct: 633 LELEEGARLVAQAAEHAPKNAAADALHK----LAQLLSEPDQAH---RLKLLLDPVTALV 685 Query: 613 IDRLTELAGARAFLMRSATEYPLETERPAATFSSWYELFPRSQSGDAAVHGTFNDVIARL 672 + + + R F + S YPL+ ER A F+SWYELFPRS S D HGTF+DVI RL Sbjct: 686 MAQASHY---RPFYVESVA-YPLDVERAGAAFASWYELFPRSASDDEQRHGTFDDVIRRL 741 Query: 673 PAIRAMGFDTLYFPPIHPIGKKNRKGKNNSLTPGDDDPGSPYAIGSEEGGHDAIHPQLGT 732 AIR MGFD LYFPPIHPIG+ NRKG+NNSL DDPGSPYAIG+ EGGHDAIHPQLGT Sbjct: 742 GAIRGMGFDVLYFPPIHPIGRTNRKGRNNSLRAEPDDPGSPYAIGAAEGGHDAIHPQLGT 801 Query: 733 LDDFLRLRDAAAAHGLELALDFAIQCAPDHPWLKEHPGWFSWRPDGSMRYAENPPKKYQD 792 L+DF RL AA GLELALDFAIQC+PDHPWL+EHPGWF+WRPDGS++YAENPPKKY+D Sbjct: 802 LEDFRRLMRAAREKGLELALDFAIQCSPDHPWLREHPGWFAWRPDGSIKYAENPPKKYED 861 Query: 793 IVNVDFYAEDAIPDLWIALRDIVLYWVQQGVNVFRVDNPHTKPFPFWEWMIAAVRSRYPQ 852 IVNVDFYA+DA P LW ALRD+V++W +GV +FRVDNPHTKP PFWEWMI VR+RYP Sbjct: 862 IVNVDFYADDARPGLWTALRDVVMFWADEGVRLFRVDNPHTKPLPFWEWMIGEVRARYPD 921 Query: 853 VIFLSEAFTRPKPMYRLAKVGFSQSYTYFTWRHDKQEFIDYLEELTGSPDGDTRPRDFFR 912 +FLSEAFTRPK MYRLAKVGFSQSYTYFTWRH KQEFIDYL ELT +P R++FR Sbjct: 922 TVFLSEAFTRPKVMYRLAKVGFSQSYTYFTWRHTKQEFIDYLTELTRTP-----VREYFR 976 Query: 913 PHFFVNTPDINPYFLQRSGRAGFVIRAALAATLSGLWGVYSGFELCEAQPVPGKEEYLDS 972 PHFFVNTPDINP FLQ SGR F+IRAALAATLSGLWGVY+GFELCE VPGKEEYLDS Sbjct: 977 PHFFVNTPDINPVFLQTSGRPAFLIRAALAATLSGLWGVYNGFELCEGAAVPGKEEYLDS 1036 Query: 973 EKYQVRAWDWQRPGNIIEDIARLNRIRRENPALQTHLGVHFLPSSNDKVLYFVKSTAPAY 1032 EKYQ+RAWD RPGNI+++IA LNRIRR NPAL +HLGV F P+S D++L+F KST P Sbjct: 1037 EKYQLRAWDHNRPGNIVKEIALLNRIRRANPALHSHLGVTFQPASGDRILFFSKSTPPVL 1096 Query: 1033 SV-VDAVDDRL----------RFGDNVILVAINLDPFNEQSGDIDIPLYGFGLGDDSRVQ 1081 ++ L RFGDN + VAINLDP + DI++PL+ +GL D + Sbjct: 1097 PARLETAPAELAEPANALSPDRFGDNTVFVAINLDPHHAHESDIELPLWQWGLPDHGALM 1156 Query: 1082 VEDLLTDRRFNWTGRRHQLSLNPDKNPYGIWRLR 1115 E+L+ +R W G+ + L P+ +P+ IWR+R Sbjct: 1157 AENLVNGQRTVWRGKWQHIRLEPE-HPFAIWRVR 1189 >ref|NP_521802.1| putative alpha-amylase-related protein [Ralstonia solanacearum GMI1000] emb|CAD17392.1| putative alpha-amylase-related protein [Ralstonia solanacearum GMI1000] Length = 1201 Score = 973 bits (2514), Expect = 0.0, Method: Compositional matrix adjust. Identities = 578/1174 (49%), Positives = 696/1174 (59%), Gaps = 98/1174 (8%) Query: 5 AAAAFSPRIYCIDPTLVGG-GGWSDLFARCAGMGFDHVLLCGDGLIAGLFAGEDSHV--D 61 +AAAF+P I + P G W L R A +GFDHVL+ + AG + V D Sbjct: 53 SAAAFTPAICYLHPLQQGPLDQWGGLLDRIAALGFDHVLIAPPFAVG---AGGNVFVTRD 109 Query: 62 NVESPIADYGMASLDQLEELAHLATEHRLRLLLDLSLTSVHGSDQLLRSHPDWFAVAGAA 121 + A G + L LA + L LL+DL + V L HP WF Sbjct: 110 HARLHAALGGGPAHAALGRLADACRQRGLTLLMDLVIDRVATDAPLRADHPGWFHCR--- 166 Query: 122 SDASAHGDHLPDPRRPQP-------RFHAPEIAEALLECWHMRLAMLIDAGVAGFLCHDP 174 + HGD L DPR P R AEA+ W +L G+ GF C P Sbjct: 167 ---AEHGDAL-DPRLPPSHLEVASFRHDDAHCAEAIGAWWMQQLRAWTALGIGGFRCEAP 222 Query: 175 AGLPGNFWCRLVERTREQRSC--CQFLAWTPGCSHPQLESLADCGFDAVFSSGAWWDFRA 232 A LP W L+ TRE +FLAWTPG +E+LAD GFDA FSS WWDFRA Sbjct: 223 ARLPAARWHPLIAATREGAGARPVRFLAWTPGLDGRAVEALADAGFDATFSSLRWWDFRA 282 Query: 233 PWYFREHEKLVAIAPVLGFPEDPFGPRAARVHGWHDKETRRLGAIRALRFAALSGSGWLM 292 W EH +L A+AP + E PFG R + G D TR R L A + G+GWLM Sbjct: 283 GWMVEEHARLAAVAPPIATVEAPFGTRYGQAFG--DAMTRERAYRRMLDVADVLGAGWLM 340 Query: 293 PMGFEFGLTEALGIAHNAAASTRLTTPQQFAQACAESDFNLDAEVRAANQAMSAGGFR-- 350 P GFE G + P A + F+L VR N + A Sbjct: 341 PFGFERGALLPMLPDRG--------DPTDAQWIDAHAAFDLSDAVRDVNATLRAAQAAGT 392 Query: 351 ------LRAVSAPEAPVLLLARDVG-------VHQLIAINPRL-----GQVARLADGDWQ 392 LR ++ P+A + L R G L+ INP L Q R G Sbjct: 393 VAARADLRMLTGPDARMSALLRADGPDLRADDAATLLVINPDLHTGVAAQADRFLPGVAG 452 Query: 393 QRLDGRIVDWPPE----REFILQPGEIALIELQTPP-----------------PVAADLS 431 G +D E R+ L+ AL E+ PP P+ A Sbjct: 453 GFTRGVALDLLTEPAGSRDEALRAKARALAEIDLPPGGVQAWRVFRSRPVRWRPIVAPGG 512 Query: 432 RRKKP-------VAEKVRTGRIAIEQVSPAVDGGRFAVKRTVGQSFTISADIFMDGHDHL 484 +R P V RIAIE V+PAVD GRFAVKR VG + AD+ MDGHD L Sbjct: 513 QRAIPGDADSGRAGTAVAAPRIAIEHVAPAVDDGRFAVKRLVGDDIVVEADVLMDGHDQL 572 Query: 485 AAAVLVRAADEKQWQRIPLQHVGNDRWQASLSLPRLGRHYFCIEAWSDVFETYRDGLKKK 544 A + RA D+ WQR+P+ +GNDRW+ + RLGRH + + AW D F T+R L+ K Sbjct: 573 AVELQWRADDDDAWQRVPMVPLGNDRWRGAFRPERLGRHAYAVAAWRDAFGTFRAELETK 632 Query: 545 SAAGLNVDLELEEGRLLVANLAEQALENGIDGAVLEGGGDLLKSLSGKNGNHNGNRLAVL 604 AAG+ V +ELEEG LVA+ A+ A ++ GA L DLL++ +RL +L Sbjct: 633 HAAGVEVAVELEEGAQLVAHAADHAPKDAPVGA-LHQLADLLRA----TAPDPAHRLKLL 687 Query: 605 LADDTARQIDRLTELAGARAFLMRSATEYPLETERPAATFSSWYELFPRSQSGDAAVHGT 664 L TA + + + R F +RSA YPL+ ER A F+SWYELFPRS SGDA HGT Sbjct: 688 LDPATAHVMAQASHF---RPFHVRSAP-YPLDVERSGAAFASWYELFPRSASGDAQRHGT 743 Query: 665 FNDVIARLPAIRAMGFDTLYFPPIHPIGKKNRKGKNNSLTPGDDDPGSPYAIGSEEGGHD 724 F DV RL IRAMGFD LYFPPIHPIG+ +RKG+NNSL DDPGSPYAIG+ EGGHD Sbjct: 744 FRDVARRLGVIRAMGFDVLYFPPIHPIGRTHRKGRNNSLRAEPDDPGSPYAIGAAEGGHD 803 Query: 725 AIHPQLGTLDDFLRLRDAAAAHGLELALDFAIQCAPDHPWLKEHPGWFSWRPDGSMRYAE 784 AIHPQLGTLDDF RLRDAA GLELALDFAIQC+PDHPWL+ HPGWF+WRPDGS+RYAE Sbjct: 804 AIHPQLGTLDDFRRLRDAARGQGLELALDFAIQCSPDHPWLRAHPGWFAWRPDGSVRYAE 863 Query: 785 NPPKKYQDIVNVDFYAEDAIPDLWIALRDIVLYWVQQGVNVFRVDNPHTKPFPFWEWMIA 844 NPPKKY+DIVNVDFYA A P LW+ALRD+V++W +GV +FRVDNPHTKP PFWEWMI Sbjct: 864 NPPKKYEDIVNVDFYAASAKPALWLALRDVVMFWADEGVRLFRVDNPHTKPLPFWEWMIG 923 Query: 845 AVRSRYPQVIFLSEAFTRPKPMYRLAKVGFSQSYTYFTWRHDKQEFIDYLEELTGSPDGD 904 VR+RYP IFLSEAFTRPK MYRLAKVGFSQSYTYFTWRH KQEFIDYL ELT +P Sbjct: 924 EVRARYPDAIFLSEAFTRPKVMYRLAKVGFSQSYTYFTWRHGKQEFIDYLTELTRTPV-- 981 Query: 905 TRPRDFFRPHFFVNTPDINPYFLQRSGRAGFVIRAALAATLSGLWGVYSGFELCEAQPVP 964 R++FRPHFFVNTPDINP FLQ SGR GF+IRAALAATLSGLWGVY+GFELCE Q +P Sbjct: 982 ---REYFRPHFFVNTPDINPVFLQTSGRPGFLIRAALAATLSGLWGVYNGFELCEGQALP 1038 Query: 965 GKEEYLDSEKYQVRAWDWQRPGNIIEDIARLNRIRRENPALQTHLGVHFLPSSNDKVLYF 1024 GKEEYLD+EKYQ+RAWD RPGNI+ +IA LNRIRR NPAL +HLGV F P+S + +LYF Sbjct: 1039 GKEEYLDAEKYQLRAWDHDRPGNIVAEIALLNRIRRANPALHSHLGVQFQPASGEGILYF 1098 Query: 1025 VKSTA---PAYSVVDAVDDRLRFGDNVILVAINLDPFNEQSGDIDIPLYGFGLGDDSRVQ 1081 KST P + A RFGDN +LVAI+LDP + Q DI++PL+ +GL D + Sbjct: 1099 SKSTPAPLPTHPAELADAAPERFGDNTLLVAISLDPHHPQEADIELPLWQWGLPDHGALM 1158 Query: 1082 VEDLLTDRRFNWTGRRHQLSLNPDKNPYGIWRLR 1115 E+L+ RF W G+R + L P++ P+ IWR+R Sbjct: 1159 AENLVDGARFVWRGKRQHVRLAPEQ-PFAIWRVR 1191 >ref|YP_001892471.1| alpha amylase catalytic region [Ralstonia pickettii 12J] ref|YP_002983973.1| alpha amylase catalytic subunit [Ralstonia pickettii 12D] gb|ACD29044.1| alpha amylase catalytic region [Ralstonia pickettii 12J] gb|ACS65301.1| alpha amylase catalytic region [Ralstonia pickettii 12D] Length = 1196 Score = 960 bits (2482), Expect = 0.0, Method: Compositional matrix adjust. Identities = 559/1168 (47%), Positives = 696/1168 (59%), Gaps = 93/1168 (7%) Query: 9 FSPRIYCIDPTLVGG-GGWSDLFARCAGMGFDHVLLC---GDGLIAGLFAGEDSHVDNVE 64 ++P I + P +G W + R A +GFDHVL+ G +F D + + Sbjct: 51 YAPAICYVHPLQLGRLDRWDGVLDRIASLGFDHVLMAPPFAVGAAGNVFVTRD----HAQ 106 Query: 65 SPIADYGMASLDQLEELAHLATEHRLRLLLDLSLTSVHGSDQLLRSHPDWFAVAGAASDA 124 A G + L LA L L LDL + V L HPDWFA DA Sbjct: 107 LHAALGGGPADAALATLAEGCRARGLTLQLDLVIDRVAIDAPLRADHPDWFASHAEDGDA 166 Query: 125 SAHGDHLPDPRRPQP-------RFHAPEIAEALLECWHMRLAMLIDAGVAGFLCHDPAGL 177 DPR P R P A+A+L W +L G+ GF C PA L Sbjct: 167 L-------DPRLPPSHLEVASFRHDDPRCADAILMWWTQQLRAWAALGIGGFRCEAPARL 219 Query: 178 PGNFWCRLVERTREQRSCCQFLAWTPGCSHPQLESLADCGFDAVFSSGAWWDFRAPWYFR 237 P W L+ R+ +FLAWTPG +++LA GFDA FSS WWDFRA W Sbjct: 220 PAARWRTLIADVRKDVGVIRFLAWTPGMDGAAVDALAGAGFDATFSSLRWWDFRAGWMVE 279 Query: 238 EHEKLVAIAPVLGFPEDPFGPRAARVHGWHDKETRRLGAIRALRFAALSGSGWLMPMGFE 297 EH +L A+AP + E PFG R + G D R R L A +GWLMP+GFE Sbjct: 280 EHARLAAVAPPIAAVEAPFGTRYGQAFG--DAMIRERAYRRLLHVADTLDAGWLMPLGFE 337 Query: 298 FGLTEALGIAHNAAASTRLTTPQQFAQACAESDFNLDAEVRAANQAMS-AGGFRLRAVSA 356 G + +A S + A +++ +++A +RAA + + A LR ++ Sbjct: 338 RGALLPM-LAERGDPSDQQWIDAHAAFDLSDAVRDVNATIRAARETNTVAPHAELRMLTG 396 Query: 357 PEAPVLLLARDVG-------VHQLIAINPRLGQ-VARLAD-------GDWQQRLDGRIVD 401 P+AP L R G L INP L V+ AD G + + + ++ Sbjct: 397 PDAPATALLRADGPDLRAGDAATLTVINPDLYMGVSVHADHFLPGVAGGFTRGVALDLLT 456 Query: 402 WP-------------PEREFILQPGEIAL------IELQTPPPVAADLSRR------KKP 436 P P E L PG++ + ++TP V S R K+P Sbjct: 457 EPVGSCDDTLRARARPLAEIDLLPGDVQVWRVFRNAPVRTPAAVKPAKSLRTKAGEAKEP 516 Query: 437 VAEKVRTGRIAIEQVSPAVDGGRFAVKRTVGQSFTISADIFMDGHDHLAAAVLVRAADEK 496 V + + RI IEQV P+V+ GRFAVKR VG + AD+ MDGHD LA +L RA DE Sbjct: 517 VNAAIASPRIGIEQVQPSVEDGRFAVKRLVGDDIIVEADVLMDGHDKLAVHLLWRAQDED 576 Query: 497 QWQRIPLQHVGNDRWQASLSLPRLGRHYFCIEAWSDVFETYRDGLKKKSAAGLNVDLELE 556 WQ +P+ +GNDRW+ + RLGRH + + AW D F TYR L+KK AG+ V LELE Sbjct: 577 TWQHVPMMPLGNDRWRGAFRPERLGRHVYAVAAWRDAFGTYRSELEKKHTAGVEVTLELE 636 Query: 557 EGRLLVANLAEQALENGIDGAVLEGGGDLLKSLSGKNGNHNGNRLAVLLADDTARQIDRL 616 EG LVA AE A + A ++ L + L+ + H RL +LL D + + Sbjct: 637 EGARLVALAAEHAPND----APVDALHKLARLLAEADQAH---RLKLLL-DPVTEHV--M 686 Query: 617 TELAGARAFLMRSATEYPLETERPAATFSSWYELFPRSQSGDAAVHGTFNDVIARLPAIR 676 + + R F + S YPL+ ER A F+SWY+LFPRS S D HGTF+DVI RL AIR Sbjct: 687 AQASHYRPFYVESVA-YPLDVERAGAAFASWYKLFPRSASDDEQRHGTFDDVIRRLGAIR 745 Query: 677 AMGFDTLYFPPIHPIGKKNRKGKNNSLTPGDDDPGSPYAIGSEEGGHDAIHPQLGTLDDF 736 MGFD LYFPPIHPIG+ NRKG+NNSL DDPGSPYAIG+ EGGHDAIHPQLGTL+DF Sbjct: 746 GMGFDVLYFPPIHPIGRTNRKGRNNSLRAEPDDPGSPYAIGATEGGHDAIHPQLGTLEDF 805 Query: 737 LRLRDAAAAHGLELALDFAIQCAPDHPWLKEHPGWFSWRPDGSMRYAENPPKKYQDIVNV 796 RL AA GLELALDFAIQC+PDHPWL+EHPGWF+WRPDGS++YAENPPKKY+DIVNV Sbjct: 806 RRLMRAAREKGLELALDFAIQCSPDHPWLREHPGWFAWRPDGSIKYAENPPKKYEDIVNV 865 Query: 797 DFYAEDAIPDLWIALRDIVLYWVQQGVNVFRVDNPHTKPFPFWEWMIAAVRSRYPQVIFL 856 DFYA+DA P LW ALRD+V++W +GV +FRVDNPHTKP PFWEWMI VR+RYP +FL Sbjct: 866 DFYADDAKPGLWTALRDVVMFWADEGVRLFRVDNPHTKPLPFWEWMIGEVRARYPDTVFL 925 Query: 857 SEAFTRPKPMYRLAKVGFSQSYTYFTWRHDKQEFIDYLEELTGSPDGDTRPRDFFRPHFF 916 SEAFTRPK MYRLAKVGFSQSYTYFTWRH KQEFIDYL ELT +P R++FRPHFF Sbjct: 926 SEAFTRPKVMYRLAKVGFSQSYTYFTWRHTKQEFIDYLTELTRTP-----VREYFRPHFF 980 Query: 917 VNTPDINPYFLQRSGRAGFVIRAALAATLSGLWGVYSGFELCEAQPVPGKEEYLDSEKYQ 976 VNTPDINP FLQ SGR GF+IRAALAATLSGLWGVY+GFELCE VPGKEEYLDSEKYQ Sbjct: 981 VNTPDINPVFLQTSGRPGFLIRAALAATLSGLWGVYNGFELCEGAAVPGKEEYLDSEKYQ 1040 Query: 977 VRAWDWQRPGNIIEDIARLNRIRRENPALQTHLGVHFLPSSNDKVLYFVKSTAPAYSV-- 1034 +RAWD RPGNI+++IA LNRIRR NPAL +HLGV F P+S D++L+F KST P Sbjct: 1041 LRAWDHNRPGNIVKEIALLNRIRRANPALHSHLGVTFQPASGDRILFFSKSTPPVLPAQL 1100 Query: 1035 -------VDAVDDRLRFGDNVILVAINLDPFNEQSGDIDIPLYGFGLGDDSRVQVEDLLT 1087 +A+ RFGDN + VAINLDP + DI++PL+ +GL D + E+L+ Sbjct: 1101 ETSQAEPANALSPD-RFGDNTVFVAINLDPHHAHESDIELPLWQWGLPDHGALMAENLVN 1159 Query: 1088 DRRFNWTGRRHQLSLNPDKNPYGIWRLR 1115 +R W G+ + L P+ +P+ IWR+R Sbjct: 1160 GQRTVWRGKWQHIRLEPE-HPFAIWRVR 1186 >ref|YP_001860554.1| alpha amylase catalytic region [Burkholderia phymatum STM815] gb|ACC73508.1| alpha amylase catalytic region [Burkholderia phymatum STM815] Length = 1144 Score = 958 bits (2476), Expect = 0.0, Method: Compositional matrix adjust. Identities = 554/1163 (47%), Positives = 678/1163 (58%), Gaps = 102/1163 (8%) Query: 5 AAAAFSPRIYCIDPTLVGG-GGWSDLFARCAGMGFDHVLLCGDGLIAGLFA-GEDSHVDN 62 A A++PRIY + LVG W FA A +GFDHVL I GLF G+ H Sbjct: 3 APHAYAPRIYFVHSPLVGRLEAWPAQFAHAASLGFDHVL------IGGLFQPGQAGHAQI 56 Query: 63 VESPIADYGMASLDQ-----LEELAHLATEHRLRLLLDLSLTSVHGSDQLLRSHPDWF-- 115 V + + DQ L+ LA A++H L LL+DL + QL R H DWF Sbjct: 57 VSDHARLHPALATDQPAHEGLQRLAETASQHGLTLLVDLVIDRAAADGQLYREHRDWFHP 116 Query: 116 ---AVAGAASDASAHGDHLPDPRRPQPRFHAPEIAEALLECWHMRLAMLIDAGVAGFLCH 172 A + H D++ F +A L++ W L L DAG+ GF Sbjct: 117 FEPEEARLDPRHARHEDNVA-----YANFSNEHVAGPLIDWWAHTLDALADAGIGGFRFD 171 Query: 173 DPAGLPGNFWCRLVERTREQRS-CCQFLAWTPGCSHPQLESLADCGFDAVFSSGAWWDFR 231 P +P W RL++ R R +FLA TPG + +L GFDAVFSS WWDFR Sbjct: 172 SPHRVPVFAWRRLLDAVRASRHPSTRFLAATPGLVRSDIVALESVGFDAVFSSSRWWDFR 231 Query: 232 APWYFREHEKLVAIAPVLGFPEDPFGPR-AARVHGWHDKETRRLGAIRALRFAALSGSGW 290 APW EH L + FPE P+G R AA + HD RAL AA +G+GW Sbjct: 232 APWMLEEHAVLTRAGSPIAFPEAPYGTRLAADLEQVHDSAIVERAYRRALMTAASTGTGW 291 Query: 291 LMPMGFEFGLTEALGIAHNAAASTRLTTPQQFAQACAESDFNLDAEVRAANQ------AM 344 LMPMGFE+G+ E + S L +A + + F+L VR AN ++ Sbjct: 292 LMPMGFEYGIAEPI--------SHTLGDAGHYASLRSAARFDLSERVRHANAIARDTWSL 343 Query: 345 SAGGFRLRAVSAPEAPVLLLAR-------DVGVHQLIAINPRLGQVARLADGDWQQRLDG 397 G LR++S P+ LL R D LIAINP LG R+ + + G Sbjct: 344 QTNG-ELRSLSGPDTHAALLLRGDKRDLRDADEAVLIAINPELGTPVRVDPARFLDGVPG 402 Query: 398 RIVDW-----------PPEREFILQPGEIALIELQTPPPVA--------ADLSRRKKPVA 438 + P F L PG L PV + + K V Sbjct: 403 GFTRFVPLGTETRGAPQPLTPFTLPPGACRLFRAVAERPVVLAPPIDKQSATTSGHKSVL 462 Query: 439 EKVRTGRIAIEQVSPAVDGGRFAVKRTVGQSFTISADIFMDGHDHLAAAVLVRAADEKQW 498 + + T R+AIE VSP+VD GRF KR VG+ ++A +F +GHD +AAAV+ RAADE W Sbjct: 463 DAIATSRVAIESVSPSVDHGRFPAKRIVGERVHVTAAVFAEGHDKIAAAVIWRAADETAW 522 Query: 499 QRIPL---QHVGNDRWQASLSLPRLGRHYFCIEAWSDVFETYRDGLKKKSAAGLNVDLEL 555 P+ Q GND W+A ++L RLGRH + + AW D F + D ++KK A +VDLE+ Sbjct: 523 HETPMTPAQPAGNDIWEARIALERLGRHEYTVIAWRDDFASLVDHMQKKLKADQSVDLEV 582 Query: 556 EEGR-LLVANLAEQALENGIDGAVLEGGGDLLKSLSGKNGNHNGNRLAVLLADDTARQID 614 EE + L LAE G LE ++K + G RLA++LA TA Sbjct: 583 EEAKHLFALALAEAETAEGSRPQQLEA---IVKEFMKAD---MGERLAIVLAPTTAEAF- 635 Query: 615 RLTELAGARAFLMRSATEYPLETERPAATFSSWYELFPRSQSGDAAVHGTFNDVIARLPA 674 A R L R Y ++ ER A F SWYE+FPRS S D HG F+DVI +LP Sbjct: 636 ---AAARHRPGLSRDPIVYRIDAERAGARFGSWYEIFPRSMSDDEHRHGNFDDVIRKLPR 692 Query: 675 IRAMGFDTLYFPPIHPIGKKNRKGKNNSLTPGDDDPGSPYAIGSEEGGHDAIHPQLGTLD 734 IR MGFD LYFPPIHPIG NRKG+NN+LTPG DD GSPYAIG GGHDA+HPQLGTLD Sbjct: 693 IRDMGFDVLYFPPIHPIGIANRKGRNNTLTPGPDDVGSPYAIGGAAGGHDAVHPQLGTLD 752 Query: 735 DFLRLRDAAAAHGLELALDFAIQCAPDHPWLKEHPGWFSWRPDGSMRYAENPPKKYQDIV 794 DF R+ AA HGLE+ALDFAIQC+PDHPWLK+HP WF+WRPDG++RYAENPPKKYQDIV Sbjct: 753 DFKRMLAAAHEHGLEIALDFAIQCSPDHPWLKQHPTWFAWRPDGTLRYAENPPKKYQDIV 812 Query: 795 NVDFYAEDAIPDLWIALRDIVLYWVQQGVNVFRVDNPHTKPFPFWEWMIAAVRSRYPQVI 854 N DFYA DA P LWIALRD++L+W+ GV +FRVDNPHTKPFPFWEWMI VR+R+P VI Sbjct: 813 NPDFYARDAKPALWIALRDVILFWIDAGVRIFRVDNPHTKPFPFWEWMINDVRARHPDVI 872 Query: 855 FLSEAFTRPKPMYRLAKVGFSQSYTYFTWRHDKQEFIDYLEELTGSPDGDTRPRDFFRPH 914 FLSEAFTRP+ M RLAK+GFSQSYTYFTWR K++F +YL ELT T RDFFRP+ Sbjct: 873 FLSEAFTRPRVMARLAKLGFSQSYTYFTWRESKRDFTEYLTELT-----QTSLRDFFRPN 927 Query: 915 FFVNTPDINPYFLQRSGRAGFVIRAALAATLSGLWGVYSGFELCEAQPVPGKEEYLDSEK 974 FFVNTPDINP LQR GR GF+IRAALA LSGLWGVYSGFELCEA +P EEYLDSEK Sbjct: 928 FFVNTPDINPRHLQRQGRPGFLIRAALATMLSGLWGVYSGFELCEAAALPNSEEYLDSEK 987 Query: 975 YQVRAWDWQRPGNIIEDIARLNRIRRENPALQTHLGVHFLPSSNDKVLYFVKSTAPAYSV 1034 YQ+RAWDW RPGNI+ +I LNRIRR NPAL THL + FLP+ ND +L+F K+T Sbjct: 988 YQIRAWDWNRPGNIVREITELNRIRRTNPALHTHLNLTFLPAHNDHILFFEKATEAR--- 1044 Query: 1035 VDAVDDRLRFGDNVILVAINLDPFNEQSGDIDIPLYGF---GLGDDSRVQVEDLLTDRRF 1091 DNVI+VAINLDP NEQ DI++ F G+ D S + V D +T RF Sbjct: 1045 -----------DNVIVVAINLDPHNEQGADIELSWSTFNRWGVHDHSTIDVIDQMTGERF 1093 Query: 1092 NWTGRRHQLSLNPDKNPYGIWRL 1114 W GR + L+P P+ IWR+ Sbjct: 1094 QWQGRWQHVRLDPGTRPFAIWRI 1116