BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0022.p (444 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum serop... 626 e-177 ref|YP_001424132.2| pyruvate dehydrogenase [cytochrome] [Coxiell... 319 8e-85 ref|YP_746170.1| pyruvate dehydrogenase [Granulibacter bethesden... 318 1e-84 ref|YP_003114119.1| thiamine pyrophosphate protein TPP binding d... 313 5e-83 ref|ZP_07294123.1| putative pyruvate dehydrogenase [Streptomyces... 308 1e-81 >ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] gb|ADJ63768.1| pyruvate dehydrogenase protein [Herbaspirillum seropedicae SmR1] Length = 564 Score = 626 bits (1614), Expect = e-177, Method: Compositional matrix adjust. Identities = 309/456 (67%), Positives = 364/456 (79%), Gaps = 18/456 (3%) Query: 1 LQENNPDLLFRDVAAYTQTIIDPAQAVQVAHQAIAQAYHQRGVAHISIPADVIGVKLPGT 60 LQEN+P+LLFRDVAAY+Q I P QA V HQAIAQAY QRGVAH++IPADVIG K G Sbjct: 112 LQENSPELLFRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSG- 170 Query: 61 PSVASLNTLRPQPEVTPPESEIAHAAQLINAAKSVAIFVGNGARSSIADIAVLAEKLQAP 120 SV SL+TLR + ++PP ++I AA LINAA SVAIF GNG R +I ++ LA KLQAP Sbjct: 171 -SVPSLHTLRERTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAP 229 Query: 121 ILHTFRAKDMLPYDHPHWIGGVGLIGGAPGMDAMRDAGLLLMLGTDYPYSEFLPTRTQTV 180 ++HTFR KD+ YDHPHWIGGVGLIGGAPG DA+ A ++LMLG+DYPYSEFLPT+++ + Sbjct: 230 VMHTFRGKDLASYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVI 289 Query: 181 QIDERGFVLGRRMPVDLGITGSVGPAVTQLLPQVQGKTDNAFLRKVGEHRKQWNETLDKH 240 QIDERGFVLGRR PV LGITGSV PAV QLL +V+GKTD AFL++ HR++WN LD Sbjct: 290 QIDERGFVLGRRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSA 349 Query: 241 AVLPADAGKGPVKPQYLARRLSDRAAEDAVFVVDTGVVTLWCGNWIRQSGRQRILASFNN 300 A + K +KPQ +AR +SD A +DA FVVDTGVVTLWCGNWIRQ G QRILASFNN Sbjct: 350 AEIKRHHKK--IKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNN 407 Query: 301 AAVGTSLGQGNGIQALDRDRQVIVAVGDGGFTMLLGEFMTAVEHKLPVKVVVFNNREWGL 360 AAVGTSLGQ NGIQALDR+RQV+VAVGDGGFTMLLGEFMT+VEH+LPVKVVVFNNREWGL Sbjct: 408 AAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGL 467 Query: 361 VHLEMEEAGLPAFE--------------KXGAQGFTAKTPAQLEEVLEPFLAAPGPAILN 406 VHLEMEEAG+PAFE G QGF+ +TP +LE L+ LAAPGPA+L+ Sbjct: 468 VHLEMEEAGMPAFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLD 527 Query: 407 VFIDPSELPIMPHIKLDQIWHFGMAKVKEAMISMGG 442 VFIDP ELP+MPHIK++QIW FG+AKV+E++I+M G Sbjct: 528 VFIDPDELPVMPHIKMEQIWRFGLAKVRESLIAMKG 563 >ref|YP_001424132.2| pyruvate dehydrogenase [cytochrome] [Coxiella burnetii Dugway 5J108-111] gb|ABS76858.2| pyruvate dehydrogenase (cytochrome) [Coxiella burnetii Dugway 5J108-111] Length = 563 Score = 319 bits (817), Expect = 8e-85, Method: Compositional matrix adjust. Identities = 168/449 (37%), Positives = 260/449 (57%), Gaps = 22/449 (4%) Query: 2 QENNPDLLFRDVAAYTQTIIDPAQAVQVAHQAIAQAYHQRGVAHISIPADVIGVKLPGTP 61 QE N D LF DV+AY +++I P Q ++ A+ A ++GVAH+SIP DV P Sbjct: 116 QEVNLDRLFDDVSAYNESLIVPEQMPRLGLLAVQTAIARQGVAHLSIPTDV----GPSKE 171 Query: 62 SVASLN--TLRPQPEVTPPESEIAHAAQLINAAKSVAIFVGNGARSSIADIAVLAEKLQA 119 SL+ + + P ++ + A +N + V IF+G GAR + +I LA+KL++ Sbjct: 172 INWSLDHPVFQKYQQPVPAPQDLENIATALNRSDKVTIFIGWGARGATQEILDLAQKLKS 231 Query: 120 PILHTFRAKDMLPYDHPHWIGGVGLIGGAPGMDAMRDAGLLLMLGTDYPYSEFLPTRTQT 179 P++H + K ++ ++P+W GGVG++G GM+AM LLLMLGTD+PY +F P Sbjct: 232 PVIHALKGKAVIANENPYWAGGVGMLGSTCGMEAMESCELLLMLGTDFPYRQFYPKGKTI 291 Query: 180 VQIDERGFVLGRRMPVDLGITGSVGPAVTQLLPQVQGKTDNAFLRKVGEHRKQWNETLDK 239 +QID LG+R V +G+ G V +VT LLP V+ K + L++ RK+W E + K Sbjct: 292 IQIDTLPEHLGKRCNVKMGVVGDVKASVTALLPMVKEKKSDKHLKRTQRKRKEWEERMLK 351 Query: 240 HAVLPADAGKGPVKPQYLARRLSDRAAEDAVFVVDTGVVTLWCGNWIRQSGRQRILASFN 299 A+ P K ++PQ +A ++ A+++AVF DTG +W I G + +ASFN Sbjct: 352 RAIRPL---KNSIRPQAVAHLINHYASDNAVFTADTGETIIWMARQIEMKGERDFIASFN 408 Query: 300 NAAVGTSLGQGNGIQALDRDRQVIVAVGDGGFTMLLGEFMTAVEHKLPVKVVVFNNREWG 359 A++ + Q G+QALDR RQV+ GDGGFTML+G+F+TAV H+LP+K VFNN + G Sbjct: 409 LASMANAFAQAIGVQALDRSRQVVAMCGDGGFTMLMGDFITAVSHQLPIKAFVFNNGKLG 468 Query: 360 LVHLEMEEAGLPAF-------------EKXGAQGFTAKTPAQLEEVLEPFLAAPGPAILN 406 LV +EME +G P + + G +G ++ QL+ ++ GP +++ Sbjct: 469 LVKMEMEASGYPEWGVSLHNPNLADCAKAMGGEGIRVESHDQLKNAVKRAFEIDGPVLVD 528 Query: 407 VFIDPSELPIMPHIKLDQIWHFGMAKVKE 435 V +P+EL + P +K + W F ++K+KE Sbjct: 529 VLTNPNELIVPPKLKPAEAWGFSISKMKE 557 >ref|YP_746170.1| pyruvate dehydrogenase [Granulibacter bethesdensis CGDNIH1] gb|ABI63247.1| pyruvate oxidase [Granulibacter bethesdensis CGDNIH1] Length = 577 Score = 318 bits (816), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 170/455 (37%), Positives = 258/455 (56%), Gaps = 17/455 (3%) Query: 1 LQENNPDLLFRDVAAYTQTIIDPAQAVQVAHQAIAQAYHQRGVAHISIPADVIGVKLPGT 60 QE P+ LFR+ + Y +T+ DP Q + + AI A +GVA + IP DV +P + Sbjct: 113 FQETRPEALFRECSVYCETVSDPDQMPRTLNTAIRAAIGHQGVAVVVIPGDVAMKTVP-S 171 Query: 61 PSVASLNTLRPQPEVTPPESEIAHA-AQLINAAKSVAIFVGNGARSSIADIAVLAEKLQA 119 P V++ L P+P VT P E +A L+ AA V + G G R + ++ LAE L+A Sbjct: 172 PVVSTKTALCPEPPVTSPSEESLNALTALLEAAGKVTLMCGRGCRGAHDEVVALAEHLKA 231 Query: 120 PILHTFRAKDMLPYDHPHWIGGVGLIGGAPGMDAMRDAGLLLMLGTDYPYSEFLPTRTQT 179 PI+H K+ + YD+P+ +G GLIG + G AM +LLMLGTD+PY +F P + Sbjct: 232 PIVHALGGKEWIEYDNPYDVGMTGLIGFSSGYYAMESCDMLLMLGTDFPYRQFFPEEAKI 291 Query: 180 VQIDERGFVLGRRMPVDLGITGSVGPAVTQLLPQVQGKTDNAFLRKVGEHRKQWNETLDK 239 Q+D R LGRR +DLG+ G V ++ LLP+++ K D A L +H + + LD Sbjct: 292 AQVDLRPENLGRRTRIDLGVVGDVSATISALLPRLKAKQDTAHLDTCRQHYLKARKGLDD 351 Query: 240 HAVLPADAGKGPVKPQYLARRLSDRAAEDAVFVVDTGVVTLWCGNWIRQSGRQRILASFN 299 A AG+ P+ PQ++AR +S+ A++DAVF D G T+W +++ +GR+R+L SF Sbjct: 352 LA--DGKAGRPPIHPQHVARLISEHASDDAVFACDVGTPTIWAARYLKMNGRRRLLGSFA 409 Query: 300 NAAVGTSLGQGNGIQALDRDRQVIVAVGDGGFTMLLGEFMTAVEHKLPVKVVVFNNREWG 359 + ++ +L Q G Q +RQV+ GDGGF ML+G+F+T V+H+LPVK+VVFNN G Sbjct: 410 HGSMANALPQAIGAQVSAPERQVVSMSGDGGFAMLMGDFLTLVQHRLPVKIVVFNNGTLG 469 Query: 360 LVHLEMEEAGL---------PAF----EKXGAQGFTAKTPAQLEEVLEPFLAAPGPAILN 406 V +EM+ AGL P F G + P +L+E + L PGPA+L+ Sbjct: 470 FVEMEMKAAGLLESGVALDNPDFAAMARACGVYARRVEDPGKLDEAVRDVLNHPGPALLD 529 Query: 407 VFIDPSELPIMPHIKLDQIWHFGMAKVKEAMISMG 441 ++ EL + P + ++Q+ F + ++ M G Sbjct: 530 AVVNRQELSMPPKVSIEQVKGFSLYMLRAVMSGRG 564 >ref|YP_003114119.1| thiamine pyrophosphate protein TPP binding domain-containing protein [Catenulispora acidiphila DSM 44928] gb|ACU72278.1| thiamine pyrophosphate protein TPP binding domain protein [Catenulispora acidiphila DSM 44928] Length = 576 Score = 313 bits (801), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 178/469 (37%), Positives = 261/469 (55%), Gaps = 34/469 (7%) Query: 1 LQENNPDLLFRDVAAYTQTIIDPAQAVQVAHQAIAQAYHQRGVAHISIPADVIGVKLPGT 60 QE + D +FRDVAA+ T+ +Q +V QA+ AY GV+ +++P D+ G+ Sbjct: 111 FQEVDNDAVFRDVAAFRHTVTSASQMPRVLEQAVQTAYATPGVSVLTLPGDI------GS 164 Query: 61 PSVA---SLNTLRPQPEVTPPESEIAHAAQLINAAKSVAIFVGNGARSSIADIAVLAEKL 117 VA +++ R +TP + EI A +L++ AK+V + VG GAR S A + LA++L Sbjct: 165 AEVAKDSAVHITRVPARLTPDDDEITRAVRLLDDAKTVTMLVGAGARESRASVLQLADRL 224 Query: 118 QAPILHTFRAKDMLPYDHPHWIGGVGLIGGAPGMDAMRDAGLLLMLGTDYPYSEFLPTRT 177 AP++ T +AK+ L D+P IG GLIG +A AG LLM+GTD+PY ++LP T Sbjct: 225 AAPMVLTLKAKEGLEDDNPFQIGQSGLIGNPATREAFESAGALLMIGTDFPYPDWLPRST 284 Query: 178 QTVQIDERGFVLGRRMPVDLGITGSVGPAVTQLLPQVQGKTDNAFLRKV--------GEH 229 TVQID R +GRR PVD+G+ G G ++ LL +V+ K D + L K G Sbjct: 285 PTVQIDTRAGHIGRRTPVDVGVVGDAGLSIAALLNRVRSKDDRSHLEKARSSYEDWQGHQ 344 Query: 230 RKQWNETLDKHAVLPA----DAGKGPVKPQYLARRLSDRAAEDAVFVVDTGVVTLWCGNW 285 R+ + D+ V D + ++P+ LA + AAED VF DTG+ T+W Sbjct: 345 RRLTDPEFDQSLVGKVRSWLDNTEDKIRPEALATLIDTHAAEDTVFTTDTGMSTVWLARC 404 Query: 286 IRQSGRQRILASFNNAAVGTSLGQGNGIQALDRDRQVIVAVGDGGFTMLLGEFMTAVEHK 345 + G +R++ SFN ++ +L G ALDR RQV+ GDGG TMLLG+ +TAV + Sbjct: 405 VTMRGSRRLIGSFNLGSMANALPHALGAAALDRQRQVVAFCGDGGLTMLLGDVLTAVAYD 464 Query: 346 LPVKVVVFNNREWGLVHLEMEEAGLPAF-------------EKXGAQGFTAKTPAQLEEV 392 LPVK++VF+N G+V LE E+ GLP F G P LE Sbjct: 465 LPVKLIVFDNGRLGMVKLEQEQGGLPEFGTELANPDLAAVATAMGMPAARVTEPEALEAA 524 Query: 393 LEPFLAAPGPAILNVFIDPSELPIMPHIKLDQIWHFGMAKVKEAMISMG 441 ++ LA+PGP +L+V +P E+ + P +DQ W F +AK+KE ++S G Sbjct: 525 VQAALASPGPYLLDVVTNPEEIALPPKTSIDQAWGFAIAKMKEGIVSRG 573 >ref|ZP_07294123.1| putative pyruvate dehydrogenase [Streptomyces hygroscopicus ATCC 53653] gb|EFL22492.1| putative pyruvate dehydrogenase [Streptomyces himastatinicus ATCC 53653] Length = 547 Score = 308 bits (789), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 172/465 (36%), Positives = 260/465 (55%), Gaps = 34/465 (7%) Query: 1 LQENNPDLLFRDVAAYTQTIIDPAQAVQVAHQAIAQAYHQRGVAHISIPADVIGVKLPGT 60 QE +PD LFR+ + Y++ I P Q +V AI A +RGV+ +S+P D+ P Sbjct: 80 FQETHPDQLFRECSHYSELISSPQQMPRVLQTAIQHAVGRRGVSVVSLPGDIAARPAPER 139 Query: 61 PSVASLNTLRPQPEVTPPESEIAHAAQLINAAKSVAIFVGNGARSSIADIAVLAEKLQAP 120 +L T RP V P ++EI A++++ A V +F G+G + ++ AE+++AP Sbjct: 140 AEEHALVTSRPT--VRPGDTEIDALARMVDEADRVTLFCGSGTAGAHEEVMAFAERVKAP 197 Query: 121 ILHTFRAKDMLPYDHPHWIGGVGLIGGAPGMDAMRDAGLLLMLGTDYPYSEFLPTRTQTV 180 + H R K+ + YD+P+ +G GL+G +A + LLL+LGTD+PY+ FLP +TV Sbjct: 198 VGHALRGKEHIQYDNPYDVGMSGLLGYGAAYEATNECDLLLLLGTDFPYNAFLPDDVRTV 257 Query: 181 QIDERGFVLGRRMPVDLGITGSVGPAVTQLLPQVQGKTDNAFL-RKVGEHR-------KQ 232 Q+D R LGRR +DL + G V + L P+V+ KTD FL R + +H K Sbjct: 258 QVDVRPEHLGRRSKLDLAVWGDVRETLRCLTPKVRPKTDRRFLDRMLKKHAEALEGVVKA 317 Query: 233 WNETLDKHAVLPADAGKGPVKPQYLARRLSDRAAEDAVFVVDTGVVTLWCGNWIRQSGRQ 292 + ++KH P+ P+Y+A L + AA+DA+F VDTG+ +W ++ +GR+ Sbjct: 318 YTRKVEKHI---------PIHPEYVASILDEEAADDAIFTVDTGMCNVWAARYLTPNGRR 368 Query: 293 RILASFNNAAVGTSLGQGNGIQALDRDRQVIVAVGDGGFTMLLGEFMTAVEHKLPVKVVV 352 R++ SF + ++ +L Q G Q LDR RQVI GDGGFTML+G+F+T V++ LPVKV++ Sbjct: 369 RVIGSFTHGSMANALPQAIGAQFLDRRRQVISMSGDGGFTMLMGDFLTLVQYDLPVKVIL 428 Query: 353 FNNREWGLVHLEMEEAGLPAF-------------EKXGAQGFTAKTPAQLEEVLEPFLAA 399 FNN G+V LEM +GLPA+ GA + P L L L Sbjct: 429 FNNSSLGMVELEMLVSGLPAYGTGNHNPNFAQLARAAGAYAERVEKPKHLRSALRTALHH 488 Query: 400 PGPAILNVFIDPSELPIMPHIKLDQIWHFGMAKVKEAMISMGGAG 444 GPA+++V DP+ L I P IK + + F ++ K M+ GG G Sbjct: 489 KGPALVDVVTDPNALSIPPKIKAEMVTGFALSASK--MVLDGGVG 531