BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0271.p (471 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EGD06150.1| pyruvate kinase [Burkholderia sp. TJI49] 693 0.0 ref|YP_729117.1| pyruvate kinase [Ralstonia eutropha H16] >gi|11... 672 0.0 ref|YP_002007560.1| pyruvate kinase [Cupriavidus taiwanensis LMG... 671 0.0 ref|YP_004681125.1| pyruvate kinase Pyk [Cupriavidus necator N-1... 670 0.0 ref|ZP_07674149.1| pyruvate kinase [Ralstonia sp. 5_7_47FAA] >gi... 658 0.0 >gb|EGD06150.1| pyruvate kinase [Burkholderia sp. TJI49] Length = 437 Score = 693 bits (1788), Expect = 0.0, Method: Compositional matrix adjust. Identities = 341/436 (78%), Positives = 378/436 (86%) Query: 35 LNFSHGSHEDHRQRLQLIRDIESEKQRPIGVLLDLQGPKLRIATFLAGPVLLETGAAFHL 94 +NFSHG+H+DHR+RL +IR IE + RPI VLLDLQGPKLRI TF GP++LE GAAF L Sbjct: 1 MNFSHGTHDDHRRRLDIIRSIEEDSGRPISVLLDLQGPKLRIGTFANGPIMLEKGAAFRL 60 Query: 95 DLDVATPGTVSRVALPHPEIFAALKVGTRLLLDDGRIRLKVLRCGLDFAETLVEVGGLLS 154 DLD TPG +RV+LPHPEIF+ALK T LLLDDGRIRL+V RCG FAET V VGG LS Sbjct: 61 DLDAQTPGDNARVSLPHPEIFSALKPQTNLLLDDGRIRLRVERCGAGFAETTVVVGGALS 120 Query: 155 ERKGVNVPDVVLPVSAMTAKDRRDLEFGLELGVDWVALSFVQRPEDIQEVRAIAGERAAI 214 ERKGVNVPDVVLPVSAMT KDRRDL FG+ LG+DW+ALSFVQR +DI EVR I G+ I Sbjct: 121 ERKGVNVPDVVLPVSAMTEKDRRDLAFGMTLGIDWLALSFVQRVDDIVEVRRIVGDSVGI 180 Query: 215 VAKLEKPAAITFLDEIVSEADAVMVARGDLGVELPAEQVPALQKRIVRACRRAGKPVIVA 274 VAKLEKPAAI+ LD IV EADAVMVARGDLGVE+PAE VPA+QKRIVRACR+AGKPVIVA Sbjct: 181 VAKLEKPAAISSLDAIVEEADAVMVARGDLGVEMPAEHVPAIQKRIVRACRKAGKPVIVA 240 Query: 275 TQMLESMVSAPVPTRAEASDVATAIYDGADAVMLSAESASGAWPVEAVRTMDLIIGRTEQ 334 TQMLESMV+APVPTRAEASDVATAIYDGADAVMLSAESASG +PVEAVR MDLII +TE+ Sbjct: 241 TQMLESMVTAPVPTRAEASDVATAIYDGADAVMLSAESASGGYPVEAVRMMDLIISQTER 300 Query: 335 DPYYREAIAASHSAPKAEFSDAIGYAMRHVAGLLDAAATVAYTTSGYSALRMARERPSTP 394 DPYYRE IAASH+AP+A SDAIGYAMRHV GLL+A ATVAYT SGYSAL M+RERPS P Sbjct: 301 DPYYREVIAASHAAPRANTSDAIGYAMRHVTGLLNAVATVAYTASGYSALCMSRERPSAP 360 Query: 395 ILGMTPNRTTARRLALVWGIHPVLCHEVVDAGEVSELACRTVSAEGFGGPGQTVVISAGM 454 I+GMTP TARRLALVWG+HPVLC EVVD EVS+LAC+TV AEGFG G+TVVISAGM Sbjct: 361 IVGMTPQNGTARRLALVWGVHPVLCKEVVDVLEVSDLACQTVHAEGFGEQGETVVISAGM 420 Query: 455 PFGTTGTTNLLRLAQI 470 PFGT+G+TNLLR+AQI Sbjct: 421 PFGTSGSTNLLRIAQI 436 >ref|YP_729117.1| pyruvate kinase [Ralstonia eutropha H16] emb|CAJ95752.1| pyruvate kinase [Ralstonia eutropha H16] Length = 472 Score = 672 bits (1733), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/454 (73%), Positives = 378/454 (83%), Gaps = 2/454 (0%) Query: 19 STPAX--SLRGAGVDVFRLNFSHGSHEDHRQRLQLIRDIESEKQRPIGVLLDLQGPKLRI 76 STP +L AG DVFRLNFSHG+HEDHRQR + IR +E ++ RPIGVLLDLQGPKLRI Sbjct: 18 STPELIDALFEAGADVFRLNFSHGTHEDHRQRFEAIRAVERKRGRPIGVLLDLQGPKLRI 77 Query: 77 ATFLAGPVLLETGAAFHLDLDVATPGTVSRVALPHPEIFAALKVGTRLLLDDGRIRLKVL 136 TF GPV L GA F LDLD A PG+ SRV LPHPEIFAAL G LLLDDG+IRL+V Sbjct: 78 GTFADGPVQLAAGAGFRLDLDSAAPGSASRVCLPHPEIFAALSEGVELLLDDGKIRLRVE 137 Query: 137 RCGLDFAETLVEVGGLLSERKGVNVPDVVLPVSAMTAKDRRDLEFGLELGVDWVALSFVQ 196 +CG FAET V GG LS+RKGVNVPD VLP+SAMT KDRRDL+FGL LG DW+ALSFVQ Sbjct: 138 QCGAGFAETTVVNGGTLSDRKGVNVPDAVLPLSAMTEKDRRDLDFGLALGADWIALSFVQ 197 Query: 197 RPEDIQEVRAIAGERAAIVAKLEKPAAITFLDEIVSEADAVMVARGDLGVELPAEQVPAL 256 RPEDI+E++AI RA IVAKLEKPAAI LD IV+EADA+MVARGDLGVE+PAEQVP+L Sbjct: 198 RPEDIREIKAIVNGRAGIVAKLEKPAAIQSLDLIVAEADAIMVARGDLGVEMPAEQVPSL 257 Query: 257 QKRIVRACRRAGKPVIVATQMLESMVSAPVPTRAEASDVATAIYDGADAVMLSAESASGA 316 QK+IVRACR+AGKPVIVATQMLESM++APVPTRAEASDVATAIYDGADAVMLSAESASG Sbjct: 258 QKQIVRACRKAGKPVIVATQMLESMIAAPVPTRAEASDVATAIYDGADAVMLSAESASGR 317 Query: 317 WPVEAVRTMDLIIGRTEQDPYYREAIAASHSAPKAEFSDAIGYAMRHVAGLLDAAATVAY 376 +PVEAVR M+ II RTE DP YR+AI ASH+ P+A+ +DAIGYA+RH+ GLL ATVAY Sbjct: 318 YPVEAVRMMNSIISRTESDPLYRDAIEASHTPPRADAADAIGYAVRHIVGLLRVPATVAY 377 Query: 377 TTSGYSALRMARERPSTPILGMTPNRTTARRLALVWGIHPVLCHEVVDAGEVSELACRTV 436 T+SGYSALRMARERP PILGMTP TARRL L WG+H VLCHEV+D E+++LA RTV Sbjct: 378 TSSGYSALRMARERPQVPILGMTPRMATARRLTLTWGVHAVLCHEVLDVPEMTDLASRTV 437 Query: 437 SAEGFGGPGQTVVISAGMPFGTTGTTNLLRLAQI 470 AEGFG GQ+VVISAG+PF GTTNLLR+AQI Sbjct: 438 LAEGFGEAGQSVVISAGLPFLVAGTTNLLRIAQI 471 >ref|YP_002007560.1| pyruvate kinase [Cupriavidus taiwanensis LMG 19424] emb|CAQ71503.1| PYRUVATE KINASE; tartrate degradation [Cupriavidus taiwanensis LMG 19424] Length = 472 Score = 671 bits (1732), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/447 (74%), Positives = 376/447 (84%) Query: 24 SLRGAGVDVFRLNFSHGSHEDHRQRLQLIRDIESEKQRPIGVLLDLQGPKLRIATFLAGP 83 +L AG DVFRLNFSHGSH+DHRQRL IR IE E+ RPIGVLLDLQGPKLRI F GP Sbjct: 25 ALFDAGADVFRLNFSHGSHDDHRQRLDTIRAIERERGRPIGVLLDLQGPKLRIGAFAEGP 84 Query: 84 VLLETGAAFHLDLDVATPGTVSRVALPHPEIFAALKVGTRLLLDDGRIRLKVLRCGLDFA 143 V L A+F LDLD PG+ +RV+LPHPEIFAAL+ G+ LLLDDG+IRL+V RCG DFA Sbjct: 85 VQLAAEASFRLDLDQDVPGSAARVSLPHPEIFAALREGSELLLDDGKIRLRVDRCGSDFA 144 Query: 144 ETLVEVGGLLSERKGVNVPDVVLPVSAMTAKDRRDLEFGLELGVDWVALSFVQRPEDIQE 203 +T V GG LS+RKGVNVP VVLP+SAMT KDRRDLEFGL LGVDW+ALSFVQRPEDI E Sbjct: 145 DTTVVNGGTLSDRKGVNVPGVVLPLSAMTDKDRRDLEFGLALGVDWIALSFVQRPEDIHE 204 Query: 204 VRAIAGERAAIVAKLEKPAAITFLDEIVSEADAVMVARGDLGVELPAEQVPALQKRIVRA 263 ++AI +RA IVAKLEKPAAI LD IV+EADAVMVARGDLGVE+PAEQVP+LQK IVRA Sbjct: 205 IKAIVQDRAGIVAKLEKPAAIQSLDAIVAEADAVMVARGDLGVEMPAEQVPSLQKHIVRA 264 Query: 264 CRRAGKPVIVATQMLESMVSAPVPTRAEASDVATAIYDGADAVMLSAESASGAWPVEAVR 323 CR+AGKPVIVATQMLESMV+APVPTRAEASDVATA+YDGADAVMLSAESASG +PVEAV+ Sbjct: 265 CRKAGKPVIVATQMLESMVAAPVPTRAEASDVATAVYDGADAVMLSAESASGRYPVEAVK 324 Query: 324 TMDLIIGRTEQDPYYREAIAASHSAPKAEFSDAIGYAMRHVAGLLDAAATVAYTTSGYSA 383 M+ II RTE DP Y +AI ASH+ P+AE +DAIGYA+RHVAGLL ATVAYT+SGYSA Sbjct: 325 MMNSIITRTESDPLYHDAIQASHTPPRAEAADAIGYAVRHVAGLLRVPATVAYTSSGYSA 384 Query: 384 LRMARERPSTPILGMTPNRTTARRLALVWGIHPVLCHEVVDAGEVSELACRTVSAEGFGG 443 LRMARERP PILGMTP TARRLAL WG+H VLCHEVVD E++ELA RTV E FG Sbjct: 385 LRMARERPEVPILGMTPRIATARRLALAWGVHAVLCHEVVDVREMTELASRTVLRERFGE 444 Query: 444 PGQTVVISAGMPFGTTGTTNLLRLAQI 470 GQ++VISAG+PF GTTNLLR+AQ+ Sbjct: 445 RGQSIVISAGLPFTVAGTTNLLRIAQV 471 >ref|YP_004681125.1| pyruvate kinase Pyk [Cupriavidus necator N-1] gb|AEI79893.1| pyruvate kinase Pyk [Cupriavidus necator N-1] Length = 472 Score = 670 bits (1729), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/443 (74%), Positives = 372/443 (83%) Query: 28 AGVDVFRLNFSHGSHEDHRQRLQLIRDIESEKQRPIGVLLDLQGPKLRIATFLAGPVLLE 87 AG DVFRLNFSHG+H+DHRQR + IR +E E RPIGVLLDLQGPKLRI TF GPV L Sbjct: 29 AGADVFRLNFSHGTHDDHRQRFEAIRAVERELGRPIGVLLDLQGPKLRIGTFADGPVQLA 88 Query: 88 TGAAFHLDLDVATPGTVSRVALPHPEIFAALKVGTRLLLDDGRIRLKVLRCGLDFAETLV 147 GA F LDLD A PG+ SRV LPHPEIFAAL G LLLDDG+ RL+V +CG DFAET V Sbjct: 89 AGAGFRLDLDSAAPGSASRVCLPHPEIFAALSEGVELLLDDGKTRLRVGQCGADFAETTV 148 Query: 148 EVGGLLSERKGVNVPDVVLPVSAMTAKDRRDLEFGLELGVDWVALSFVQRPEDIQEVRAI 207 GG LS+RKGVNVPD VLP+SAMT KDRRDL+FGL LG DW+ALSFVQRPEDI+E++AI Sbjct: 149 VNGGTLSDRKGVNVPDAVLPLSAMTEKDRRDLDFGLALGADWIALSFVQRPEDIREIKAI 208 Query: 208 AGERAAIVAKLEKPAAITFLDEIVSEADAVMVARGDLGVELPAEQVPALQKRIVRACRRA 267 RA IVAKLEKPAAI LD IV+EADA+MVARGDLGVE+PAEQVP+LQK+IVRACR+A Sbjct: 209 VNGRAGIVAKLEKPAAIQSLDLIVAEADAIMVARGDLGVEMPAEQVPSLQKQIVRACRKA 268 Query: 268 GKPVIVATQMLESMVSAPVPTRAEASDVATAIYDGADAVMLSAESASGAWPVEAVRTMDL 327 GKPVIVATQMLESM++APVPTRAEASDVATAIYDGADAVMLSAESASG +PVEAVR M+ Sbjct: 269 GKPVIVATQMLESMIAAPVPTRAEASDVATAIYDGADAVMLSAESASGRYPVEAVRMMNS 328 Query: 328 IIGRTEQDPYYREAIAASHSAPKAEFSDAIGYAMRHVAGLLDAAATVAYTTSGYSALRMA 387 II RTE DP YR+AI ASH+ P+A+ +DAIGYA+RH+ GLL ATVAYT+SGYSALRMA Sbjct: 329 IISRTESDPLYRDAIEASHTPPRADAADAIGYAVRHIVGLLRVPATVAYTSSGYSALRMA 388 Query: 388 RERPSTPILGMTPNRTTARRLALVWGIHPVLCHEVVDAGEVSELACRTVSAEGFGGPGQT 447 RERP PILGMTP TARRL L WG+H VLCHEV+D E+++LA RTV AEGFG GQ+ Sbjct: 389 RERPQVPILGMTPRMATARRLTLTWGVHAVLCHEVLDVPEMTDLASRTVLAEGFGEAGQS 448 Query: 448 VVISAGMPFGTTGTTNLLRLAQI 470 +VISAG+PF GTTNLLR+AQI Sbjct: 449 IVISAGLPFLVAGTTNLLRIAQI 471 >ref|ZP_07674149.1| pyruvate kinase [Ralstonia sp. 5_7_47FAA] gb|EFP67580.1| pyruvate kinase [Ralstonia sp. 5_7_47FAA] Length = 472 Score = 658 bits (1697), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/443 (73%), Positives = 371/443 (83%) Query: 28 AGVDVFRLNFSHGSHEDHRQRLQLIRDIESEKQRPIGVLLDLQGPKLRIATFLAGPVLLE 87 AG DVFRLNFSHG+H+DHRQRL IR IE E+ RPIGVLLDLQGPKLRI TF GPV L Sbjct: 29 AGADVFRLNFSHGTHDDHRQRLDTIRVIERERGRPIGVLLDLQGPKLRIGTFENGPVQLA 88 Query: 88 TGAAFHLDLDVATPGTVSRVALPHPEIFAALKVGTRLLLDDGRIRLKVLRCGLDFAETLV 147 +G AF LDLD PG+ RV +PHPEIFAAL GT LLLDDG+IRL+V CG DFA+T V Sbjct: 89 SGDAFRLDLDRDAPGSRQRVGMPHPEIFAALHKGTELLLDDGKIRLRVDTCGADFADTTV 148 Query: 148 EVGGLLSERKGVNVPDVVLPVSAMTAKDRRDLEFGLELGVDWVALSFVQRPEDIQEVRAI 207 GG LS+RKGVNVP VVLP+SA+T KDRRDL+FGL LG+DW+ALSFVQRPEDI+E++AI Sbjct: 149 VNGGTLSDRKGVNVPGVVLPLSALTEKDRRDLDFGLTLGIDWIALSFVQRPEDIRELKAI 208 Query: 208 AGERAAIVAKLEKPAAITFLDEIVSEADAVMVARGDLGVELPAEQVPALQKRIVRACRRA 267 RA+IVAKLEKPAAI L+ I+ EADAVMVARGDLGVE+PAEQVP++QK+IVR CR+A Sbjct: 209 VQGRASIVAKLEKPAAIQSLEAIIDEADAVMVARGDLGVEMPAEQVPSIQKQIVRVCRKA 268 Query: 268 GKPVIVATQMLESMVSAPVPTRAEASDVATAIYDGADAVMLSAESASGAWPVEAVRTMDL 327 GKPVIVATQMLESM++APVPTRAEASDVATA+YDGADAVMLSAESASG +P+EAV MD Sbjct: 269 GKPVIVATQMLESMITAPVPTRAEASDVATAVYDGADAVMLSAESASGRYPIEAVTMMDS 328 Query: 328 IIGRTEQDPYYREAIAASHSAPKAEFSDAIGYAMRHVAGLLDAAATVAYTTSGYSALRMA 387 II RTE DP Y +AI ASH+ P+A+ +DAIGYA+RHVAGLL ATVAYT+SGYSALRMA Sbjct: 329 IIRRTESDPLYHDAIQASHTPPRADAADAIGYAVRHVAGLLKVPATVAYTSSGYSALRMA 388 Query: 388 RERPSTPILGMTPNRTTARRLALVWGIHPVLCHEVVDAGEVSELACRTVSAEGFGGPGQT 447 RERP PILGMTP TARRLAL WG+H VLCHEVVD E++ELA RTV E FG GQ+ Sbjct: 389 RERPEVPILGMTPRMATARRLALAWGVHAVLCHEVVDVLEMTELASRTVLDEHFGERGQS 448 Query: 448 VVISAGMPFGTTGTTNLLRLAQI 470 +VISAG+PF GTTNLLR+AQ+ Sbjct: 449 IVISAGLPFTVAGTTNLLRIAQV 471