BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0258.p (397 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003978599.1| AMP-binding protein [Achromobacter xylosoxid... 503 e-140 ref|YP_004681504.1| long-chain-fatty-acid--CoA ligase LcfB [Cupr... 394 e-107 ref|YP_004125428.1| amp-dependent synthetase and ligase [Alicycl... 390 e-106 ref|YP_293347.1| AMP-dependent synthetase and ligase [Ralstonia ... 389 e-106 ref|YP_002799391.1| AMP-dependent synthetase and ligase protein ... 386 e-105 >ref|YP_003978599.1| AMP-binding protein [Achromobacter xylosoxidans A8] gb|ADP15884.1| AMP-binding enzyme family protein 11 [Achromobacter xylosoxidans A8] Length = 508 Score = 503 bits (1294), Expect = e-140, Method: Compositional matrix adjust. Identities = 244/381 (64%), Positives = 297/381 (77%), Gaps = 2/381 (0%) Query: 11 LKIVDVGTDHYLDAASGPLLDAFPIAADALAWLFYTSGTTGRPKGVMLTHANMVSMAMHF 70 +++VDV + Y AA G L LAWLFYTSGTTG+PKGVML+HANMV M ++F Sbjct: 124 VQLVDVDSARYASAAIGTALPLERRKPQELAWLFYTSGTTGKPKGVMLSHANMVGMCLNF 183 Query: 71 LIDVQPLASDDVLIHVAPMSHGSGLYSVPYFIRGGLQVIPESGGLDEAELFGLLNHYDKA 130 DVQ + D ++HVAP+SHG+GLYS+PY++ G LQ+IP+SGGLDE EL LL HY + Sbjct: 184 YADVQSVQPGDAIVHVAPLSHGAGLYSIPYWLHGALQIIPDSGGLDEDELSALLTHYRRV 243 Query: 131 SLFAAPTIVMRLIQHVRNHTGVTWPGLRCLLVGGAPFYAEDIKSAVQCFGARVAQLYGQG 190 SLFA+PTIV R+++H R G PGLR L+VGGAPFY EDIK AV C+GARVAQ+YGQG Sbjct: 244 SLFASPTIVQRMVRHARQTNGPQ-PGLRTLIVGGAPFYVEDIKDAVACYGARVAQIYGQG 302 Query: 191 ETPMTISALSADLLARAVAADDTAMLVSVGFAQTAIGITIVDADGNPVTAGTPGEVIVRG 250 E+PM+ISALSA +A AVA +D +L SVG+ Q ++ + + A G P AG GEV+V G Sbjct: 303 ESPMSISALSAAQIANAVANNDDILLGSVGYVQASMEVEVRAASGQP-AAGELGEVVVSG 361 Query: 251 PTVMTGYWGNPEATRQTIGGGALRTGDIGLIDERGLLHLKDRSKDVIISGGTNIYPREVE 310 PTVM GYW NP A+ T+ G L TGDIG++DERGLLHLKDRSKDVIISGGTNIYPREVE Sbjct: 362 PTVMQGYWNNPRASADTLADGWLHTGDIGILDERGLLHLKDRSKDVIISGGTNIYPREVE 421 Query: 311 EALLTHPAVEEVSVVGAPDPEWGESVVAFIVCHAGASAGEQELDELCIRTIARFKRPKRY 370 EAL+ H AV+EVSVVG+PDPEWGESV+AF+V GA A EQ+LD+LC+ +IARFKRPKRY Sbjct: 422 EALMRHDAVQEVSVVGSPDPEWGESVLAFVVLKPGARATEQDLDQLCLASIARFKRPKRY 481 Query: 371 VFVEKLPKNGTGKVLKRELQQ 391 VF+++LPKN TGKVLKRELQQ Sbjct: 482 VFLDELPKNSTGKVLKRELQQ 502 >ref|YP_004681504.1| long-chain-fatty-acid--CoA ligase LcfB [Cupriavidus necator N-1] gb|AEI80272.1| long-chain-fatty-acid--CoA ligase LcfB [Cupriavidus necator N-1] Length = 515 Score = 394 bits (1011), Expect = e-107, Method: Compositional matrix adjust. Identities = 210/388 (54%), Positives = 259/388 (66%), Gaps = 6/388 (1%) Query: 10 ALKIVDVGTD-HYLDAASG-PLLDAFPIAADALAWLFYTSGTTGRPKGVMLTHANMVSMA 67 A IV TD H L AA G P+ D ++ DALAWLFYTSGTTG+PKGVML+H N+ +M+ Sbjct: 117 ARTIVVGSTDYHALFAAHGIPVTD---VSEDALAWLFYTSGTTGKPKGVMLSHRNLGAMS 173 Query: 68 MHFLIDVQPLASDDVLIHVAPMSHGSGLYSVPYFIRGGLQVIPESGGLDEAELFGLLNHY 127 + V + D LIH APMSHGSGLY VP+ G QVIP SGG D AEL GL+ + Sbjct: 174 RAYASAVTRILPGDSLIHAAPMSHGSGLYVVPHMAEGASQVIPPSGGFDVAELAGLVAAH 233 Query: 128 DKASLFAAPTIVMRLIQHVRNHTGVTWPGLRCLLVGGAPFYAEDIKSAVQCFGARVAQLY 187 + S FAAPT++ RL+++ R T L+ ++ GGAP Y ED +A++C G R+AQ+Y Sbjct: 234 QRPSFFAAPTMLNRLVEYTRR-TRADLSNLKVVVCGGAPLYVEDELAAIECLGPRIAQIY 292 Query: 188 GQGETPMTISALSADLLARAVAADDTAMLVSVGFAQTAIGITIVDADGNPVTAGTPGEVI 247 GQGE+PMTI+A S+ + RA A D L SVG + I I DA + G GEV+ Sbjct: 293 GQGESPMTITAQSSHEIGRAYAVGDLKALASVGRTLPGVEIRIADAGDRALPVGEVGEVL 352 Query: 248 VRGPTVMTGYWGNPEATRQTIGGGALRTGDIGLIDERGLLHLKDRSKDVIISGGTNIYPR 307 VRGPTVM GYW NPEA+ T+ G L TGD+G +DE+G L LKDRSKD IISGG+NIYPR Sbjct: 353 VRGPTVMQGYWNNPEASVATLRNGWLHTGDVGCVDEQGQLTLKDRSKDTIISGGSNIYPR 412 Query: 308 EVEEALLTHPAVEEVSVVGAPDPEWGESVVAFIVCHAGASAGEQELDELCIRTIARFKRP 367 EVE+ALL H AV EVSV+G P WGE VVA +V AG + ELD+ C+ IARFKRP Sbjct: 413 EVEDALLAHAAVAEVSVLGRRHPVWGEEVVAVVVLKAGMRMTQSELDQWCLHRIARFKRP 472 Query: 368 KRYVFVEKLPKNGTGKVLKRELQQTLAN 395 K Y+FV LPKN GKVLK L++ LA Sbjct: 473 KAYLFVNALPKNSNGKVLKTALRELLAQ 500 >ref|YP_004125428.1| amp-dependent synthetase and ligase [Alicycliphilus denitrificans BC] gb|ADU98540.1| AMP-dependent synthetase and ligase [Alicycliphilus denitrificans BC] Length = 502 Score = 390 bits (1003), Expect = e-106, Method: Compositional matrix adjust. Identities = 200/375 (53%), Positives = 252/375 (67%), Gaps = 2/375 (0%) Query: 16 VGTDHYLDAASGPLLDAFPIAADALAWLFYTSGTTGRPKGVMLTHANMVSMAMHFLIDVQ 75 +G + AA G L A + +A LFYTSGTTGRPKGVM TH N+++M M + DV Sbjct: 127 LGDAAWEQAAQGAPLPLQECAPEDVASLFYTSGTTGRPKGVMQTHRNLLAMTMAYFTDVD 186 Query: 76 PLASDDVLIHVAPMSHGSGLYSVPYFIRGGLQVIPESGGLDEAELFGLLNHYDKASLFAA 135 + DD +++ APMSHG+GLY+ + +RG V+PESGG D AEL L + SLFAA Sbjct: 187 DVRHDDAMVYAAPMSHGAGLYNYAHMLRGARHVVPESGGFDPAELVRLAAGVGRLSLFAA 246 Query: 136 PTIVMRLIQHVRNHTGVTWPGLRCLLVGGAPFYAEDIKSAVQCFGARVAQLYGQGETPMT 195 PT+V RL++HVR+ TG G + ++ GG P Y ED++ A+ G + Q+YGQGE+PMT Sbjct: 247 PTMVHRLVEHVRS-TGADAGGFKTIVYGGGPMYVEDLRRAIDAMGQKFVQIYGQGESPMT 305 Query: 196 ISALSADLLARAVAADDTAMLVSVGFAQTAIGITIVDADGNPVTAGTPGEVIVRGPTVMT 255 I+AL+ + LA + SVG A + + + +VD PV AG PGEV+VRG TVM Sbjct: 306 ITALAREHLADTAHPRWAERIASVGVAHSCVQVRVVDGQDRPVPAGEPGEVVVRGETVMA 365 Query: 256 GYWGNPEATRQTIGGGALRTGDIGLIDERGLLHLKDRSKDVIISGGTNIYPREVEEALLT 315 GYWGN EAT QT+ GG L TGD+G +DE G L L+DRSKDVIISGG+NIYPREVEE LL Sbjct: 366 GYWGNAEATAQTLRGGWLHTGDVGQLDEHGFLTLRDRSKDVIISGGSNIYPREVEEVLLQ 425 Query: 316 HPAVEEVSVVGAPDPEWGESVVAFIVCHAGASAGEQELDELCIRTIARFKRPKRYVFVEK 375 HP V E +VVG D EWGE VVAF+V GA + ELD LC+ IARFKRPK Y +V+ Sbjct: 426 HPRVREAAVVGRRDAEWGEVVVAFLVAD-GAPVPDAELDALCLAHIARFKRPKAYRWVQA 484 Query: 376 LPKNGTGKVLKRELQ 390 LPKN GKVLK EL+ Sbjct: 485 LPKNSYGKVLKTELR 499 >ref|YP_293347.1| AMP-dependent synthetase and ligase [Ralstonia eutropha JMP134] gb|AAZ65490.1| AMP-dependent synthetase and ligase [Ralstonia eutropha JMP134] Length = 537 Score = 389 bits (998), Expect = e-106, Method: Compositional matrix adjust. Identities = 199/384 (51%), Positives = 257/384 (66%), Gaps = 1/384 (0%) Query: 12 KIVDVGTDHYLDAASGPLLDAFPIAADALAWLFYTSGTTGRPKGVMLTHANMVSMAMHFL 71 K + V + Y +GP +A DALAWLFYTSGTTG+PKGVML+H N+ +M + Sbjct: 140 KTIAVDSTDYHALFAGPGAQVADVAEDALAWLFYTSGTTGKPKGVMLSHQNLAAMYWAYS 199 Query: 72 IDVQPLASDDVLIHVAPMSHGSGLYSVPYFIRGGLQVIPESGGLDEAELFGLLNHYDKAS 131 V + D LIH APMSHGSGLY VP+ G QVIP SGG + AEL L+ + + S Sbjct: 200 SAVSHIVPGDSLIHAAPMSHGSGLYIVPHIAEGASQVIPSSGGFNAAELVELVAVHPRTS 259 Query: 132 LFAAPTIVMRLIQHVRNHTGVTWPGLRCLLVGGAPFYAEDIKSAVQCFGARVAQLYGQGE 191 LFAAPT++ RL+++VR + L+ ++ GGAP Y ED +A+ C GA++AQ+YGQGE Sbjct: 260 LFAAPTMLNRLVEYVRR-SRADLSNLKVIVCGGAPLYLEDEVAALDCLGAKIAQIYGQGE 318 Query: 192 TPMTISALSADLLARAVAADDTAMLVSVGFAQTAIGITIVDADGNPVTAGTPGEVIVRGP 251 +PMTI+A ++ ++ A AA D L SVG A + I I D +P+ G GEV+VRG Sbjct: 319 SPMTITAQTSAEISGAHAASDFGGLASVGRALPGVEIRIADTHDDPLPVGEIGEVLVRGS 378 Query: 252 TVMTGYWGNPEATRQTIGGGALRTGDIGLIDERGLLHLKDRSKDVIISGGTNIYPREVEE 311 VM GYW NPEA+ T+ G L TGD+G +D+ G L LKDRSK+ IISGG+N+YPREVE+ Sbjct: 379 AVMQGYWNNPEASAVTLRNGWLHTGDVGCMDQSGRLTLKDRSKETIISGGSNVYPREVED 438 Query: 312 ALLTHPAVEEVSVVGAPDPEWGESVVAFIVCHAGASAGEQELDELCIRTIARFKRPKRYV 371 ALLTHPAV EVSV+G P+WGE VVA +V A + ELD+ C+R IARFKRPK Y+ Sbjct: 439 ALLTHPAVAEVSVLGRSHPDWGEEVVAIVVRKARSQVTISELDQWCLRRIARFKRPKAYL 498 Query: 372 FVEKLPKNGTGKVLKRELQQTLAN 395 FV+ LP+N GKVLK L+ LA Sbjct: 499 FVDSLPRNANGKVLKAALRDLLAK 522 >ref|YP_002799391.1| AMP-dependent synthetase and ligase protein [Azotobacter vinelandii DJ] gb|ACO78416.1| AMP-dependent synthetase and ligase protein [Azotobacter vinelandii DJ] Length = 502 Score = 386 bits (992), Expect = e-105, Method: Compositional matrix adjust. Identities = 189/356 (53%), Positives = 249/356 (69%), Gaps = 1/356 (0%) Query: 38 DALAWLFYTSGTTGRPKGVMLTHANMVSMAMHFLIDVQPLASDDVLIHVAPMSHGSGLYS 97 D LAWLFYTSGTTGR KGVML+H N+++M++ + +DV P+ +DD ++ APMSHG+GLY+ Sbjct: 148 DDLAWLFYTSGTTGRAKGVMLSHGNLLAMSLCYPVDVDPVEADDAYVYAAPMSHGAGLYN 207 Query: 98 VPYFIRGGLQVIPESGGLDEAELFGLLNHYDKASLFAAPTIVMRLIQHVRNHTGVTWPGL 157 + G V+P S G D AELFGL + SLFAAPT++ R++ R G G+ Sbjct: 208 FIHVRCGARHVVPASRGFDAAELFGLAGTLGRLSLFAAPTMLKRMVGEARRR-GYAGDGI 266 Query: 158 RCLLVGGAPFYAEDIKSAVQCFGARVAQLYGQGETPMTISALSADLLARAVAADDTAMLV 217 + ++ GGAP Y D++ A+ FG R+ Q+YGQGE+PMTISAL +L+A AD + Sbjct: 267 KSIVCGGAPLYLADLREALATFGPRLIQIYGQGESPMTISALCRELIADRARADWPRLAA 326 Query: 218 SVGFAQTAIGITIVDADGNPVTAGTPGEVIVRGPTVMTGYWGNPEATRQTIGGGALRTGD 277 SVG+ Q+ + + ++DA G+P+ G PGEV+VRGP VM GYW + A+ T+ G LRTGD Sbjct: 327 SVGYPQSCVEVGVLDAAGDPLPPGRPGEVVVRGPCVMHGYWRDAAASAATLVDGWLRTGD 386 Query: 278 IGLIDERGLLHLKDRSKDVIISGGTNIYPREVEEALLTHPAVEEVSVVGAPDPEWGESVV 337 IG +D GLL L DRSKDVIISGG+NIYPREVEE L HPA+ EVSVVG PDPEWGESV Sbjct: 387 IGYLDRDGLLTLTDRSKDVIISGGSNIYPREVEEVLALHPAIAEVSVVGEPDPEWGESVA 446 Query: 338 AFIVCHAGASAGEQELDELCIRTIARFKRPKRYVFVEKLPKNGTGKVLKRELQQTL 393 AF+V G A Q L+ + +A FK+P+RY+FV +LPKNG GK+LK EL++ L Sbjct: 447 AFVVLRPGERADAQALNAWFVARLASFKKPRRYLFVRELPKNGYGKILKTELRRCL 502