BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0253.p (451 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776296.1| high affinity nitrate/nitrite transporter tr... 714 0.0 ref|YP_004750827.1| nitrate/nitrite transporter [Collimonas fung... 652 0.0 ref|YP_003906619.1| major facilitator superfamily protein [Burkh... 630 e-178 ref|YP_004228590.1| major facilitator superfamily protein [Burkh... 624 e-176 emb|CBJ40899.1| nitrate transporter; Major facilitator superfami... 612 e-173 >ref|YP_003776296.1| high affinity nitrate/nitrite transporter transmembrane protein [Herbaspirillum seropedicae SmR1] gb|ADJ64388.1| high affinity nitrate/nitrite transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 435 Score = 714 bits (1843), Expect = 0.0, Method: Compositional matrix adjust. Identities = 374/433 (86%), Positives = 402/433 (92%), Gaps = 3/433 (0%) Query: 16 SKASKIRLFSIDTPQMRAFHLTWMAFFVCFFAWFACAPLMPVIKGQLGLTVEQIANINIA 75 SKA++I LFS+ +PQMRAFHLTWMAFFVCFFAWFACAPLMPVIKG+ LT QIANINIA Sbjct: 2 SKANRIELFSLRSPQMRAFHLTWMAFFVCFFAWFACAPLMPVIKGEFHLTPGQIANINIA 61 Query: 76 AVAVTILVRLIIGPLCDRYGPRKAYTGLLLIGALPVIGVGFAQTYETFLFFRLCIGAVGA 135 AVAVTILVRLIIGP+CDR+GPRK YTGLLL GA+PVIGV FAQ+YE+FLFFRL IGAVGA Sbjct: 62 AVAVTILVRLIIGPMCDRFGPRKTYTGLLLAGAVPVIGVAFAQSYESFLFFRLLIGAVGA 121 Query: 136 SFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQGLMPLLLAAMLMLGVGDNLGWRV 195 SFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQG+MPLL+AA+LMLGV GWR Sbjct: 122 SFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQGVMPLLMAALLMLGVSQGWGWRA 181 Query: 196 ALLVPGLMMLAMAALYWHFTQDCPQGNFDELRAAGIAIESGKKGGWASFRAASANYRVWM 255 ALLVPG++ML MAALYW FTQDCP+GN+D LRAAG+ IESGK+GGWASFRAA+ANYRVWM Sbjct: 182 ALLVPGVLMLVMAALYWRFTQDCPEGNYDALRAAGVTIESGKRGGWASFRAAAANYRVWM 241 Query: 256 LFITYGACFGVEIFIHNIAAIYYVDHFGLSLKSAGIAAASFGLLALFARALGGIISDKVA 315 LFITYGACFGVEIFIHNIAAIYYV+HF LSL+SAGIAAASFGLLALFARALGG ISDKVA Sbjct: 242 LFITYGACFGVEIFIHNIAAIYYVEHFKLSLESAGIAAASFGLLALFARALGGFISDKVA 301 Query: 316 LRGGLNRRATLLFIMMLGEGLGLLWFAHAGSVTLAVTAMLCFGLFTHMACGATYALVPFI 375 L GGLNRRATLLF+MMLGEGLGLLWFAHAGSV AV AMLCFGLFTHMACGATYALVPFI Sbjct: 302 LAGGLNRRATLLFVMMLGEGLGLLWFAHAGSVVAAVIAMLCFGLFTHMACGATYALVPFI 361 Query: 376 DRKALGGVAGIIGAGGNVGAVLAGFLMKGLGDTQQTLSMLGVLVTVSALCAVAVRFTTSD 435 DRKALGGVAGIIGAGGNVGAVLAGFLMKGLG+TQQTLSMLGVLVT+SALCAVA+RFT SD Sbjct: 362 DRKALGGVAGIIGAGGNVGAVLAGFLMKGLGNTQQTLSMLGVLVTISALCAVAIRFTASD 421 Query: 436 KNDEPTPAVAANN 448 K+ E AV A+N Sbjct: 422 KSGE---AVLADN 431 >ref|YP_004750827.1| nitrate/nitrite transporter [Collimonas fungivorans Ter331] gb|AEK60004.1| Nitrate/nitrite transporter [Collimonas fungivorans Ter331] Length = 439 Score = 652 bits (1682), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/425 (83%), Positives = 388/425 (91%) Query: 15 SSKASKIRLFSIDTPQMRAFHLTWMAFFVCFFAWFACAPLMPVIKGQLGLTVEQIANINI 74 ++KA+ I+LFS+ TPQMRAFHLTWMAFFVCFFAWFACAPLMP+IKGQLGL++ QIANINI Sbjct: 2 ANKATSIQLFSLRTPQMRAFHLTWMAFFVCFFAWFACAPLMPLIKGQLGLSMAQIANINI 61 Query: 75 AAVAVTILVRLIIGPLCDRYGPRKAYTGLLLIGALPVIGVGFAQTYETFLFFRLCIGAVG 134 AAVAVTILVRL++GPLCDRYGPRK YTGLLLIGALPV GV Q+YE+FLFFRLCIGAVG Sbjct: 62 AAVAVTILVRLLVGPLCDRYGPRKTYTGLLLIGALPVFGVAMVQSYESFLFFRLCIGAVG 121 Query: 135 ASFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQGLMPLLLAAMLMLGVGDNLGWR 194 ASFVITQYHTSVMFA NVVGTANAA+AGWGNAGGG AQ LMPLL A+LMLGV + LGWR Sbjct: 122 ASFVITQYHTSVMFASNVVGTANAASAGWGNAGGGAAQALMPLLATALLMLGVSETLGWR 181 Query: 195 VALLVPGLMMLAMAALYWHFTQDCPQGNFDELRAAGIAIESGKKGGWASFRAASANYRVW 254 +ALLVPG+ ML MAALYW +TQDCPQGN+ +LRA G+AIE GKKGGWASFRAASANYRVW Sbjct: 182 LALLVPGVFMLIMAALYWRYTQDCPQGNYADLRARGVAIEGGKKGGWASFRAASANYRVW 241 Query: 255 MLFITYGACFGVEIFIHNIAAIYYVDHFGLSLKSAGIAAASFGLLALFARALGGIISDKV 314 +LF+TYGACFGVEIFIHNIAAIYYVDHFGLSLK AG+AAASFGLLALFARALGG +SDK Sbjct: 242 LLFVTYGACFGVEIFIHNIAAIYYVDHFGLSLKGAGMAAASFGLLALFARALGGWLSDKA 301 Query: 315 ALRGGLNRRATLLFIMMLGEGLGLLWFAHAGSVTLAVTAMLCFGLFTHMACGATYALVPF 374 ALRG L+ RA LLF++MLGEGLGLLWFAHAGSV LAV AML FGLFTHMACGATYALVPF Sbjct: 302 ALRGNLDSRAALLFVLMLGEGLGLLWFAHAGSVALAVVAMLTFGLFTHMACGATYALVPF 361 Query: 375 IDRKALGGVAGIIGAGGNVGAVLAGFLMKGLGDTQQTLSMLGVLVTVSALCAVAVRFTTS 434 IDRKALGGVAGIIGAGGNVGAV AGFLMKGLGDTQQTL+MLG LVT+SALCA+AVRF+ Sbjct: 362 IDRKALGGVAGIIGAGGNVGAVAAGFLMKGLGDTQQTLTMLGALVTISALCAIAVRFSPE 421 Query: 435 DKNDE 439 K E Sbjct: 422 HKAQE 426 >ref|YP_003906619.1| major facilitator superfamily protein [Burkholderia sp. CCGE1003] gb|ADN57328.1| major facilitator superfamily MFS_1 [Burkholderia sp. CCGE1003] Length = 444 Score = 630 bits (1624), Expect = e-178, Method: Compositional matrix adjust. Identities = 323/422 (76%), Positives = 362/422 (85%) Query: 15 SSKASKIRLFSIDTPQMRAFHLTWMAFFVCFFAWFACAPLMPVIKGQLGLTVEQIANINI 74 + KA +I LFS+ TP MRAFHLTWMAFFVCFFAWFACAPLMP+IK + GL+ +Q+ANINI Sbjct: 2 ADKAIRIDLFSLRTPAMRAFHLTWMAFFVCFFAWFACAPLMPLIKREFGLSADQVANINI 61 Query: 75 AAVAVTILVRLIIGPLCDRYGPRKAYTGLLLIGALPVIGVGFAQTYETFLFFRLCIGAVG 134 AAVAVTILVRL++GP+CDRYGPRK Y+GLLLIGALPV+G + YE+FL RL IGAVG Sbjct: 62 AAVAVTILVRLVVGPMCDRYGPRKVYSGLLLIGALPVLGAAVSTGYESFLLCRLLIGAVG 121 Query: 135 ASFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQGLMPLLLAAMLMLGVGDNLGWR 194 ASFVITQYHTS+MFAPNVVGTANA TAGWGN G G AQ L+PLLLAA+++LGVG++ WR Sbjct: 122 ASFVITQYHTSMMFAPNVVGTANATTAGWGNTGAGAAQALIPLLLAAIIVLGVGESSAWR 181 Query: 195 VALLVPGLMMLAMAALYWHFTQDCPQGNFDELRAAGIAIESGKKGGWASFRAASANYRVW 254 +ALLVPGL M MAA YW FTQDCPQGNF ELRA GI I+ GKKGGWASFR A ANYRVW Sbjct: 182 LALLVPGLAMPVMAAFYWRFTQDCPQGNFSELRAQGITIDGGKKGGWASFRRACANYRVW 241 Query: 255 MLFITYGACFGVEIFIHNIAAIYYVDHFGLSLKSAGIAAASFGLLALFARALGGIISDKV 314 MLFITY ACFGVE+FIHNIAAIYYVDHF LSLK AG+AA SFGLLALFARALGG +SD+ Sbjct: 242 MLFITYAACFGVEVFIHNIAAIYYVDHFHLSLKEAGMAAGSFGLLALFARALGGWVSDRA 301 Query: 315 ALRGGLNRRATLLFIMMLGEGLGLLWFAHAGSVTLAVTAMLCFGLFTHMACGATYALVPF 374 A R GL R+TLL +M+GEGLGL+ FAHA S+ A+ AML FGLFTHMACGATYALVPF Sbjct: 302 AARRGLEVRSTLLCALMIGEGLGLVGFAHANSIAPALVAMLVFGLFTHMACGATYALVPF 361 Query: 375 IDRKALGGVAGIIGAGGNVGAVLAGFLMKGLGDTQQTLSMLGVLVTVSALCAVAVRFTTS 434 IDRKALGGVAGIIGAGGNVGAV AGFLMKG+G QQTL+ LGVLVT+SALCA+AVRF+ Sbjct: 362 IDRKALGGVAGIIGAGGNVGAVAAGFLMKGIGGIQQTLATLGVLVTLSALCALAVRFSGE 421 Query: 435 DK 436 K Sbjct: 422 HK 423 >ref|YP_004228590.1| major facilitator superfamily protein [Burkholderia sp. CCGE1001] gb|ADX55530.1| major facilitator superfamily MFS_1 [Burkholderia sp. CCGE1001] Length = 450 Score = 624 bits (1609), Expect = e-176, Method: Compositional matrix adjust. Identities = 323/422 (76%), Positives = 361/422 (85%) Query: 15 SSKASKIRLFSIDTPQMRAFHLTWMAFFVCFFAWFACAPLMPVIKGQLGLTVEQIANINI 74 + KA++I LFS+ TP MRAFHLTWMAFFVCFFAWFACAPLMP+IK + GL+ +Q+ANINI Sbjct: 2 ADKATRIDLFSLRTPAMRAFHLTWMAFFVCFFAWFACAPLMPLIKREFGLSADQVANINI 61 Query: 75 AAVAVTILVRLIIGPLCDRYGPRKAYTGLLLIGALPVIGVGFAQTYETFLFFRLCIGAVG 134 AAVAVTILVRL++GP+CDRYGPRK Y+GLLL GALPV+G + Y +FL RL IGAVG Sbjct: 62 AAVAVTILVRLVVGPMCDRYGPRKVYSGLLLAGALPVLGAAVSTGYGSFLLCRLLIGAVG 121 Query: 135 ASFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQGLMPLLLAAMLMLGVGDNLGWR 194 ASFVITQYHTS+MFAPNVVGTANA TAGWGN G G AQ L+PLLLAA+++LGVG++ WR Sbjct: 122 ASFVITQYHTSMMFAPNVVGTANATTAGWGNTGAGAAQALIPLLLAAIVVLGVGESSAWR 181 Query: 195 VALLVPGLMMLAMAALYWHFTQDCPQGNFDELRAAGIAIESGKKGGWASFRAASANYRVW 254 VAL+VPGL M MAA YW FTQDCPQGNF LRA GIAI+ GKKGGWASFRAA ANYRVW Sbjct: 182 VALVVPGLAMPVMAAFYWRFTQDCPQGNFAALRAQGIAIDGGKKGGWASFRAACANYRVW 241 Query: 255 MLFITYGACFGVEIFIHNIAAIYYVDHFGLSLKSAGIAAASFGLLALFARALGGIISDKV 314 MLFITY ACFGVE+FIHNIAAIYYVDHF LSLK AG+AA SFGLLALFARALGG +SDK Sbjct: 242 MLFITYAACFGVEVFIHNIAAIYYVDHFQLSLKEAGMAAGSFGLLALFARALGGWVSDKA 301 Query: 315 ALRGGLNRRATLLFIMMLGEGLGLLWFAHAGSVTLAVTAMLCFGLFTHMACGATYALVPF 374 A GL R+TLL +++GEGLGLL FAHA SV A+ AML FGLFTHMACGATYALVPF Sbjct: 302 AASRGLEVRSTLLCALIVGEGLGLLCFAHANSVAPALVAMLVFGLFTHMACGATYALVPF 361 Query: 375 IDRKALGGVAGIIGAGGNVGAVLAGFLMKGLGDTQQTLSMLGVLVTVSALCAVAVRFTTS 434 IDRKALGGVAGIIGAGGNVGAV AGFLMKG+G QQTL+ LGVLVT+SALCA+AVRF+ Sbjct: 362 IDRKALGGVAGIIGAGGNVGAVAAGFLMKGVGGIQQTLATLGVLVTLSALCALAVRFSGE 421 Query: 435 DK 436 K Sbjct: 422 HK 423 >emb|CBJ40899.1| nitrate transporter; Major facilitator superfamily MFS_1 [Ralstonia solanacearum CMR15] Length = 441 Score = 612 bits (1578), Expect = e-173, Method: Compositional matrix adjust. Identities = 335/440 (76%), Positives = 383/440 (87%), Gaps = 5/440 (1%) Query: 15 SSKASKIRLFSIDTPQMRAFHLTWMAFFVCFFAWFACAPLMPVIKGQLGLTVEQIANINI 74 + KA++I LFS+ TPQMRAFHLTW+AFFVCF+AWFACAPLMPV+KG+ LT QIANINI Sbjct: 2 TGKATRIDLFSLRTPQMRAFHLTWLAFFVCFYAWFACAPLMPVLKGEFHLTPGQIANINI 61 Query: 75 AAVAVTILVRLIIGPLCDRYGPRKAYTGLLLIGALPVIGVGFAQTYETFLFFRLCIGAVG 134 AAVAVTILVRL++GPLCDR+GPRK YTGLL++GA+PV+GV AQ+YE FLFFRL IGA+G Sbjct: 62 AAVAVTILVRLLVGPLCDRFGPRKTYTGLLVLGAVPVLGVALAQSYEAFLFFRLAIGAIG 121 Query: 135 ASFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQGLMPLLLAAMLMLGVGDNLGWR 194 ASFVITQYHTSVMFAPNVVGTANAA AGWGNAGGGVAQG MPLLL A++M+GV +GWR Sbjct: 122 ASFVITQYHTSVMFAPNVVGTANAAAAGWGNAGGGVAQGTMPLLLTAIVMMGVTQGMGWR 181 Query: 195 VALLVPGLMMLAMAALYWHFTQDCPQGNFDELRAAGIAIESGKKGGWASFRAASANYRVW 254 +A++VPG+ ML +AALYW +TQDCP+GNFD+LRAAG IESGKKGGW SF A+ NYRVW Sbjct: 182 LAMVVPGVAMLIVAALYWRYTQDCPEGNFDDLRAAGKTIESGKKGGWGSFVTAAGNYRVW 241 Query: 255 MLFITYGACFGVEIFIHNIAAIYYVDHFGLSLKSAGIAAASFGLLALFARALGGIISDKV 314 +LF+TYGACFGVEIFIHNIAA YYVDHFGLSL +AG+AAASFGLLALFARALGGI+SD+V Sbjct: 242 LLFVTYGACFGVEIFIHNIAATYYVDHFGLSLSAAGMAAASFGLLALFARALGGIVSDRV 301 Query: 315 ALRGGLNRRATLLFIMMLGEGLGLLWFAHAGSVTLAVTAMLCFGLFTHMACGATYALVPF 374 A GL+ R LL ++M+GEGLGLL FAHAG+ +LAV AML FGLFTHMACGATYALVPF Sbjct: 302 ARSRGLDARTLLLCVLMVGEGLGLLGFAHAGTASLAVLAMLGFGLFTHMACGATYALVPF 361 Query: 375 IDRKALGGVAGIIGAGGNVGAVLAGFLMKGLGDTQQTLSMLGVLVTVSALCAVAVRFTTS 434 IDR+ALGGVAGIIGAGGNVGAV AGFLMKGLGD QQTLS+LGV+V +SA+CAVAVRFT Sbjct: 362 IDRRALGGVAGIIGAGGNVGAVAAGFLMKGLGDAQQTLSVLGVMVMLSAICAVAVRFTAE 421 Query: 435 DKNDEP-----TPAVAANNI 449 K E T A AA N+ Sbjct: 422 HKAREQALYDNTLAAAAANV 441