BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0223.p (296 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775531.1| methylglyoxal synthase [Herbaspirillum serop... 508 e-142 ref|YP_001352759.1| hypothetical protein mma_1069 [Janthinobacte... 467 e-130 ref|YP_004752235.1| methylglyoxal synthase [Collimonas fungivora... 466 e-129 ref|YP_001156407.1| hypothetical protein Pnuc_1629 [Polynucleoba... 397 e-109 ref|YP_786041.1| methylglyoxal synthase [Bordetella avium 197N] ... 326 3e-87 >ref|YP_003775531.1| methylglyoxal synthase [Herbaspirillum seropedicae SmR1] gb|ADJ63623.1| methylglyoxal synthase protein [Herbaspirillum seropedicae SmR1] Length = 295 Score = 508 bits (1309), Expect = e-142, Method: Compositional matrix adjust. Identities = 246/293 (83%), Positives = 264/293 (90%) Query: 1 MTSSSTRYRIGLAANRAHQDAPNSALVQLLQGSRARIEALQPELVVVGRTLDAIGHLGLL 60 M+S + RYRIGL ANRAHQDAP+SALVQLL GSR RIE+LQPEL+VVGRTLDAIG + LL Sbjct: 1 MSSIAPRYRIGLIANRAHQDAPDSALVQLLTGSRHRIESLQPELIVVGRTLDAIGQMNLL 60 Query: 61 PGYPHIERFPYGRQGGLMKLVSRVVDSDPARMLDAIIYLIDPVDPSSTFPEAVALKRQCV 120 PGY H+ RFPYGR GGLMKLVSR VD DP R LDA+IYLIDPVDPSSTFPEAVALKRQCV Sbjct: 61 PGYEHLVRFPYGRHGGLMKLVSRTVDEDPERTLDAVIYLIDPVDPSSTFPEAVALKRQCV 120 Query: 121 IHGKPFLSTLAGAQEWFELEAIAGGAAPDPARDGSFRLADEGIALIAHDSMKARMLALAE 180 IHGKPFLSTLAGAQEWFELEA+A GAAPD + D FRL EGIALIAHD+MK+RML LAE Sbjct: 121 IHGKPFLSTLAGAQEWFELEALANGAAPDASLDDRFRLGQEGIALIAHDAMKSRMLELAE 180 Query: 181 RHFDVLDSFGFRCATGTTGGLLNELAVKIKGEAGRNWVKPFLSGPLGGDAQIAELILEHE 240 +HF VLD F FRCATGTTGGLLN+LA+KIKGEAGR+WVKPFLSGPLGGDAQIAELILE + Sbjct: 181 KHFAVLDQFAFRCATGTTGGLLNQLAMKIKGEAGRHWVKPFLSGPLGGDAQIAELILEQQ 240 Query: 241 CRRVLFLEDPHVARQHEADIQLLERAARTVTDYASCMSDEKWGDRWLSLIKQR 293 CRRVLFLEDPHVARQHEADIQLLERAARTVT YASCMSD KWGDRWL+LI QR Sbjct: 241 CRRVLFLEDPHVARQHEADIQLLERAARTVTAYASCMSDVKWGDRWLTLIGQR 293 >ref|YP_001352759.1| hypothetical protein mma_1069 [Janthinobacterium sp. Marseille] gb|ABR88830.1| Uncharacterized conserved protein [Janthinobacterium sp. Marseille] Length = 315 Score = 467 bits (1202), Expect = e-130, Method: Compositional matrix adjust. Identities = 227/296 (76%), Positives = 260/296 (87%), Gaps = 3/296 (1%) Query: 1 MTSSSTRYRIGLAANRAHQDAPNSALVQLLQGSRARIEA-LQPELVVVGRTLDAIGHLGL 59 MT++ ++RIGLA +R HQDAPNS L +LL GSR IE LQP+L+VVGRTLDA+ HLGL Sbjct: 14 MTTTPHQFRIGLATSRTHQDAPNSVLARLLNGSRIAIEQHLQPQLIVVGRTLDAMDHLGL 73 Query: 60 LPGYPHIERFPYGRQGGLMKLVSRVVDSDPARMLDAIIYLIDPVDPSSTFPEAVALKRQC 119 L YPHIERFPYGR GGLMKLVSRVVD+DPAR LDA++YL+DPVDPSS FPEAVALKRQC Sbjct: 74 LKNYPHIERFPYGRHGGLMKLVSRVVDTDPARRLDAVMYLMDPVDPSSIFPEAVALKRQC 133 Query: 120 VIHGKPFLSTLAGAQEWFELEAIAGGAAPDPARDGSFRLADEGIALIAHDSMKARMLALA 179 VIHGKPFLSTLAGA+EW ELE+IA GAAP+P D +F L +EGIA+IAHD+MK RML +A Sbjct: 134 VIHGKPFLSTLAGAREWLELESIAIGAAPNPELDETFDLKNEGIAMIAHDAMKGRMLEIA 193 Query: 180 ERHFDVLDSFGFRCATGTTGGLLNELAVKIKG-EAGRNWVKPFLSGPLGGDAQIAELILE 238 ERHFD+LD FGFRCATGTTG LLN+LA KIKG EAGRNWVKP+LSGP+GGDAQIAELIL+ Sbjct: 194 ERHFDLLDQFGFRCATGTTGSLLNKLAQKIKGEEAGRNWVKPYLSGPMGGDAQIAELILD 253 Query: 239 H-ECRRVLFLEDPHVARQHEADIQLLERAARTVTDYASCMSDEKWGDRWLSLIKQR 293 +CRRVLFLEDPHVARQHEADIQLLERAARTVTDYA C+S+E G+RWL L+++R Sbjct: 254 KDQCRRVLFLEDPHVARQHEADIQLLERAARTVTDYALCISNENCGERWLDLLQKR 309 >ref|YP_004752235.1| methylglyoxal synthase [Collimonas fungivorans Ter331] gb|AEK61412.1| Methylglyoxal synthase [Collimonas fungivorans Ter331] Length = 333 Score = 466 bits (1199), Expect = e-129, Method: Compositional matrix adjust. Identities = 232/295 (78%), Positives = 258/295 (87%), Gaps = 3/295 (1%) Query: 2 TSSSTRYRIGLAANRAHQDAPNSALVQLLQGSRARIEA-LQPELVVVGRTLDAIGHLGLL 60 +SS +RIGLAANR+HQ P+SAL QLL GSR IE L+P+LVVVGRTLDA+ LGLL Sbjct: 28 NASSAPFRIGLAANRSHQQGPDSALAQLLTGSRHSIEQQLKPQLVVVGRTLDAMQQLGLL 87 Query: 61 PGYPHIERFPYGRQGGLMKLVSRVVDSDPARMLDAIIYLIDPVDPSSTFPEAVALKRQCV 120 YPHIERFPYGR GGLMKLVSRVVD+DPAR LDA+IYL+DPVDPSSTFPEAVALKRQCV Sbjct: 88 EHYPHIERFPYGRHGGLMKLVSRVVDADPARTLDAVIYLMDPVDPSSTFPEAVALKRQCV 147 Query: 121 IHGKPFLSTLAGAQEWFELEAIAGGAAPDPARDGSFRLADEGIALIAHDSMKARMLALAE 180 IHGKPFLSTLAGA+EW ELEA+A GAAP+PA D +F LA EGIALIAHD+MK RML +AE Sbjct: 148 IHGKPFLSTLAGAREWLELEAMAAGAAPNPALDPTFELAQEGIALIAHDAMKDRMLDIAE 207 Query: 181 RHFDVLDSFGFRCATGTTGGLLNELAVKIKGE-AGRNWVKPFLSGPLGGDAQIAELILE- 238 RHF +LD F FRCATGTTG LLN+LA KIKGE AGRNWVKPFLSGPLGGDAQIAELIL+ Sbjct: 208 RHFALLDQFAFRCATGTTGSLLNKLAQKIKGEQAGRNWVKPFLSGPLGGDAQIAELILDR 267 Query: 239 HECRRVLFLEDPHVARQHEADIQLLERAARTVTDYASCMSDEKWGDRWLSLIKQR 293 +CRRVLFLEDPHVARQHEADIQLLERAARTV DYA C+SD + G+RWL+L++QR Sbjct: 268 QQCRRVLFLEDPHVARQHEADIQLLERAARTVPDYALCISDAECGERWLTLLQQR 322 >ref|YP_001156407.1| hypothetical protein Pnuc_1629 [Polynucleobacter necessarius subsp. asymbioticus QLW-P1DMWA-1] gb|ABP34843.1| conserved hypothetical protein [Polynucleobacter necessarius subsp. asymbioticus QLW-P1DMWA-1] Length = 296 Score = 397 bits (1021), Expect = e-109, Method: Compositional matrix adjust. Identities = 198/295 (67%), Positives = 228/295 (77%), Gaps = 2/295 (0%) Query: 1 MTSSSTRYRIGLAANRAHQDAPNSALVQLLQGSRARI-EALQPELVVVGRTLDAIGHLGL 59 M + YRIGL ANRAHQD+ ++ L+QLL GS+A I E L+P VVGRT+DA+ L Sbjct: 1 MVNEVPNYRIGLVANRAHQDSEDATLIQLLNGSKASITETLKPHFSVVGRTMDAMKIYDL 60 Query: 60 LPGYPHIERFPYGRQGGLMKLVSRVVDSDPARMLDAIIYLIDPVDPSSTFPEAVALKRQC 119 LP Y IER PYG GGLM+LVSRVVD DP + L+AIIYL+DPVDPSS FPEAVALKRQC Sbjct: 61 LPDYADIERLPYGHDGGLMRLVSRVVDPDPNKALNAIIYLMDPVDPSSAFPEAVALKRQC 120 Query: 120 VIHGKPFLSTLAGAQEWFELEAIAGGAAPDPARDGSFRLADEGIALIAHDSMKARMLALA 179 VIHGKPFLSTLAGA+EW ELE++ D F ++GIALIAHD+MK ML LA Sbjct: 121 VIHGKPFLSTLAGAREWIELESMNLTGKKTKDLDPYFAYENQGIALIAHDAMKPEMLRLA 180 Query: 180 ERHFDVLDSFGFRCATGTTGGLLNELAVKIKG-EAGRNWVKPFLSGPLGGDAQIAELILE 238 E+HFD+LD F R ATGTTGGLLNELA +IKG + G WV P LSGP+GGDAQIAEL+L+ Sbjct: 181 EKHFDLLDRFKVRYATGTTGGLLNELAKQIKGSKDGEGWVTPCLSGPMGGDAQIAELVLD 240 Query: 239 HECRRVLFLEDPHVARQHEADIQLLERAARTVTDYASCMSDEKWGDRWLSLIKQR 293 +C R+LFLEDPHVARQHEADIQLLERAAR VTD+ASC SD K DRWLSL+ QR Sbjct: 241 RKCGRILFLEDPHVARQHEADIQLLERAARVVTDFASCNSDNKSADRWLSLLSQR 295 >ref|YP_786041.1| methylglyoxal synthase [Bordetella avium 197N] emb|CAJ49128.1| putative methylglyoxal synthase [Bordetella avium 197N] Length = 298 Score = 326 bits (835), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 167/297 (56%), Positives = 206/297 (69%), Gaps = 3/297 (1%) Query: 1 MTSSSTRYRIGLAANRAHQDAPNSALVQLLQGSRARIEALQPELVVVGRTLDAIGHLGLL 60 MT S R+ GLAANR H + P++AL L+ S A I + EL VGRT DAI G+L Sbjct: 1 MTVPSLRF--GLAANRLHHETPDAALFNWLRASGASIREMGIELHTVGRTHDAIVREGML 58 Query: 61 PGYPHIERFPYGRQGGLMKLVSRVVDS-DPARMLDAIIYLIDPVDPSSTFPEAVALKRQC 119 GY + R+PYGR+GGLMKLV+RV D D IYLIDPVDPSS FPEA+ALKRQC Sbjct: 59 QGYRGLIRYPYGREGGLMKLVARVTQGRDGQAPFDGAIYLIDPVDPSSIFPEALALKRQC 118 Query: 120 VIHGKPFLSTLAGAQEWFELEAIAGGAAPDPARDGSFRLADEGIALIAHDSMKARMLALA 179 + HG+PF+STLAGA EW E+E + G APDPA + FR + +A+IAHD++K M+A A Sbjct: 119 ITHGRPFISTLAGAIEWIEIERVLAGLAPDPALNRLFRFEGQTLAMIAHDALKDEMMAFA 178 Query: 180 ERHFDVLDSFGFRCATGTTGGLLNELAVKIKGEAGRNWVKPFLSGPLGGDAQIAELILEH 239 + +FDVL F R ATGTTGG LNELA + WV +LSGPLGGDAQIAEL+LEH Sbjct: 179 DANFDVLSQFARRVATGTTGGRLNELAWSRGWPKDQPWVTRYLSGPLGGDAQIAELVLEH 238 Query: 240 ECRRVLFLEDPHVARQHEADIQLLERAARTVTDYASCMSDEKWGDRWLSLIKQRQVR 296 +C+RV+F EDPHVARQHEADIQLLERA R VT+ A+C + RW +++ Q + Sbjct: 239 QCQRVIFFEDPHVARQHEADIQLLERAVRVVTESATCATSPSVARRWADAVRRHQAQ 295