BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0181.p (186 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08275620.1| Poly(3-hydroxybutyrate) depolymerase [Oxaloba... 153 1e-35 ref|YP_001890467.1| esterase, PHB depolymerase family [Burkholde... 137 8e-31 ref|YP_001860567.1| PHB depolymerase family esterase [Burkholder... 135 2e-30 ref|ZP_06842486.1| esterase, PHB depolymerase family [Burkholder... 134 9e-30 ref|YP_552499.1| esterase, PHB depolymerase [Burkholderia xenovo... 132 2e-29 >ref|ZP_08275620.1| Poly(3-hydroxybutyrate) depolymerase [Oxalobacteraceae bacterium IMCC9480] gb|EGF30913.1| Poly(3-hydroxybutyrate) depolymerase [Oxalobacteraceae bacterium IMCC9480] Length = 268 Score = 153 bits (386), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 90/183 (49%), Positives = 107/183 (58%), Gaps = 9/183 (4%) Query: 5 SPPAAMAQILALTHPQLIAAVGMHSAPLFGVSRSAVGAYGVMQHGS-TQALAPIDKLLAK 63 S AAMA ILAL HPQLIAAVG+HSAP+FG + +A+GAYGVMQ G T+ A I + Sbjct: 87 SAGAAMAHILALRHPQLIAAVGLHSAPVFGAASTALGAYGVMQRGGLTRVGAAIQAVTDT 146 Query: 64 TPG-FPGMPAILITGDDDKIVRPINQLQLAGQFLRLNHAATQAPLAPLPPVTKDFGRTSK 122 P FP MPAILI G D +VRPIN QL QF LN Q P + + Sbjct: 147 GPVPFPAMPAILIQGQQDSVVRPINFHQLEQQFCELNGLTAQQRQEP------TYRAAGR 200 Query: 123 QFPRKNSMTVRDFATGSKVLVRSVHIARLGHAWSGGDDALPFNA-KGPDAGKLMLAFFSR 181 + D+ G K L+R I +L HAWSGGD ALPFN KGPDA ++M FF+ Sbjct: 201 SARAAHGFNTLDYRVGKKTLLRLCEIHQLKHAWSGGDPALPFNERKGPDASRMMWTFFAP 260 Query: 182 HRR 184 HRR Sbjct: 261 HRR 263 >ref|YP_001890467.1| esterase, PHB depolymerase family [Burkholderia phytofirmans PsJN] gb|ACD21096.1| esterase, PHB depolymerase family [Burkholderia phytofirmans PsJN] Length = 398 Score = 137 bits (345), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 76/181 (41%), Positives = 102/181 (56%), Gaps = 10/181 (5%) Query: 5 SPPAAMAQILALTHPQLIAAVGMHSAPLFGVSRSAVGAYGVMQHGSTQALAPIDKLLAKT 64 S A + +LAL HP+ AAV +HS P FG +RS + A VM+ G+ + A + A Sbjct: 208 SAGAGLTALLALNHPEHFAAVALHSGPAFGEARSGIMAMDVMRRGARREPAELVDEAADV 267 Query: 65 PGFPGMPAILITGDDDKIVRPINQLQLAGQFLRLNHAATQAPLAPLPPVTKDFGRTSKQF 124 +PGMPA++I GD D +V P+N QLA QFLRLN Q V +D Sbjct: 268 ARYPGMPALIIHGDADHVVSPVNADQLAVQFLRLNRIVDQNGARKSGEVRED-------- 319 Query: 125 PRKNSMTVRDFATGSKVLVRSVHIARLGHAWSGGDDALPFN-AKGPDAGKLMLAFFSRHR 183 RK + +RD+ G + +VR + L HAWSGGDDA+PF+ AKGPDA ++ FF R Sbjct: 320 -RKGGVVMRDYLRGGRRVVRLCRVQGLAHAWSGGDDAVPFHSAKGPDASAMLWEFFKHQR 378 Query: 184 R 184 R Sbjct: 379 R 379 >ref|YP_001860567.1| PHB depolymerase family esterase [Burkholderia phymatum STM815] gb|ACC73521.1| esterase, PHB depolymerase family [Burkholderia phymatum STM815] Length = 398 Score = 135 bits (341), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 73/183 (39%), Positives = 104/183 (56%), Gaps = 10/183 (5%) Query: 5 SPPAAMAQILALTHPQLIAAVGMHSAPLFGVSRSAVGAYGVMQHGSTQALAPIDKLLAKT 64 S A + +LAL P+ AAV +HS P FG + S + A VM+ G+ Q + +A Sbjct: 208 SAGAGLTSLLALHFPEHFAAVALHSGPAFGEAHSGITAMDVMRRGTRQDPVALVDAVADV 267 Query: 65 PGFPGMPAILITGDDDKIVRPINQLQLAGQFLRLNHAATQAPLAPLPPVTKDFGRTSKQF 124 +PGMPAI++ G+ D++V P+N QL QFLRLN + + V +D Sbjct: 268 TAYPGMPAIILQGEADRVVAPVNAEQLTEQFLRLNGIVDAQGVRKVGDVKED-------- 319 Query: 125 PRKNSMTVRDFATGSKVLVRSVHIARLGHAWSGGDDALPFN-AKGPDAGKLMLAFFSRHR 183 RK ++T RD+ G + +VR + LGHAWSGGDDA+PF+ +KGPDA L+ FF R Sbjct: 320 -RKATVTTRDYTRGGRRIVRLCRVHGLGHAWSGGDDAVPFHSSKGPDASSLLWDFFRHQR 378 Query: 184 RKQ 186 R + Sbjct: 379 RAE 381 >ref|ZP_06842486.1| esterase, PHB depolymerase family [Burkholderia sp. Ch1-1] gb|EFG69902.1| esterase, PHB depolymerase family [Burkholderia sp. Ch1-1] Length = 398 Score = 134 bits (336), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 74/182 (40%), Positives = 104/182 (57%), Gaps = 11/182 (6%) Query: 5 SPPAAMAQILALTHPQLIAAVGMHSAPLFGVSRSAVGAYGVMQHGSTQALAPIDKLLAKT 64 S A + +LA+ +P+ AAV +HS P FG + S + A VM+ G+ + A + A Sbjct: 208 SAGAGLTALLAVKYPEHFAAVALHSGPAFGEAHSGITAMDVMRRGARREPAELVDEAANV 267 Query: 65 PGFPGMPAILITGDDDKIVRPINQLQLAGQFLRLNHAATQAPLAPLPPVTKDFGRTSKQF 124 +PGMPAI+I GD D +V P+N QLA QFLRLN + V ++ Sbjct: 268 ANYPGMPAIIIHGDADHVVAPVNADQLAVQFLRLNRIVDETGARKSGEVREE-------- 319 Query: 125 PRKNSMTVRDFATGSKVLVRSVHIARLGHAWSGGDDALPFN-AKGPDAGKLMLAFFSRHR 183 RK + +RD+ G + +VR + L HAWSGGDDA+PF+ AKGPDA +M FF RH+ Sbjct: 320 -RKGGVVMRDYVRGGRRIVRLCRVQGLAHAWSGGDDAVPFHSAKGPDASAMMWEFF-RHQ 377 Query: 184 RK 185 R+ Sbjct: 378 RR 379 >ref|YP_552499.1| esterase, PHB depolymerase [Burkholderia xenovorans LB400] gb|ABE33149.1| Esterase, PHB depolymerase [Burkholderia xenovorans LB400] Length = 398 Score = 132 bits (332), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 74/182 (40%), Positives = 104/182 (57%), Gaps = 11/182 (6%) Query: 5 SPPAAMAQILALTHPQLIAAVGMHSAPLFGVSRSAVGAYGVMQHGSTQALAPIDKLLAKT 64 S A + +LA+ +P+ AAV +HS P FG + S + A VM+ G+ + A + A Sbjct: 208 SAGAGLTALLAVKYPEHFAAVALHSGPAFGEAHSGITAMDVMRRGTRREPAELVDETADV 267 Query: 65 PGFPGMPAILITGDDDKIVRPINQLQLAGQFLRLNHAATQAPLAPLPPVTKDFGRTSKQF 124 +PGMPAI+I GD D +V PIN QLA QFLRLN + V ++ Sbjct: 268 ANYPGMPAIIIHGDADHVVAPINADQLAVQFLRLNRIVDETGARKAGEVREE-------- 319 Query: 125 PRKNSMTVRDFATGSKVLVRSVHIARLGHAWSGGDDALPFN-AKGPDAGKLMLAFFSRHR 183 RK + +RD+ G + +VR + L HAWSGGDDA+PF+ AKGPDA ++ FF RH+ Sbjct: 320 -RKGGVVMRDYVRGGRRIVRLCRVQGLAHAWSGGDDAVPFHSAKGPDASGMVWEFF-RHQ 377 Query: 184 RK 185 R+ Sbjct: 378 RR 379