BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0089.p (436 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776335.1| exported extracellular alpha-helical protein... 693 0.0 ref|YP_004753991.1| alpha-2-macroglobulin domain-containing prot... 646 0.0 ref|YP_002943272.1| alpha-2-macroglobulin [Variovorax paradoxus ... 529 e-148 ref|YP_983330.1| alpha-2-macroglobulin domain-containing protein... 529 e-148 ref|YP_004153808.1| alpha-2-macroglobulin domain-containing prot... 520 e-145 >ref|YP_003776335.1| exported extracellular alpha-helical protein [Herbaspirillum seropedicae SmR1] gb|ADJ64427.1| exported extracellular alpha-helical protein [Herbaspirillum seropedicae SmR1] Length = 1938 Score = 693 bits (1789), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/445 (74%), Positives = 382/445 (85%), Gaps = 11/445 (2%) Query: 1 LPTYLDADGLAYYFPPSESNARRGSDTLTAYLLAASNEAAYALPLQSRDKMLDGLAAFVE 60 LPTYLD DGLAYYFPPS+S ARRGSDTLTAYLLAA+NEA YA+P SRDKMLDGLAAFVE Sbjct: 1493 LPTYLDGDGLAYYFPPSDSGARRGSDTLTAYLLAATNEAGYAIPQASRDKMLDGLAAFVE 1552 Query: 61 GKLTRDFWSPKKDLDVRKLAALEALSRYHRVQPSMLGSVQINPNLWPTSAVLDWIAILQR 120 GK+ RDFWSP+KDLDVRKLAALEALSRY+RVQP+MLGS+QINPN+WPTS +LDWI ILQR Sbjct: 1553 GKVMRDFWSPQKDLDVRKLAALEALSRYNRVQPAMLGSLQINPNVWPTSGLLDWIGILQR 1612 Query: 121 VTTIPERDKRLAEADQIIRSRLNYQGTRMGFSDEAGDYWWWLMASGDSNANRLVLTMLNN 180 V IP+R K+L EADQI+R+RLN+QGTRMGFS+E GDYWWWLM+SGD+NANRL+L LNN Sbjct: 1613 VPAIPDRQKKLDEADQILRARLNFQGTRMGFSNEEGDYWWWLMSSGDANANRLILLELNN 1672 Query: 181 PAWREDMPKLISGAIQRQQRGHWNTTTANVWGSLALEKFSRAFEAEKVTGTTRVV----- 235 PAWREDMP+LI+GAI RQQRGHW+TTTANVWGSLALEKF+R FE+E VTG TR Sbjct: 1673 PAWREDMPRLIAGAIGRQQRGHWSTTTANVWGSLALEKFARKFESEPVTGATRATLEQNG 1732 Query: 236 SDAGNNAHAWSGAGVSGGKLQLPW--GTPAPGTLKFSQEGSGKPWITMQSLAAVPLKSPF 293 + A + AWSGA +GGKLQLPW G G+++ +QEG+GKPW+T+QSLAAVPLK PF Sbjct: 1733 ASAATQSLAWSGA-PNGGKLQLPWPEGNAQGGSVRLAQEGNGKPWVTLQSLAAVPLKQPF 1791 Query: 294 SSGYRITKTIVAVEQKQQGIYSRGDVLRVTLDIDAQTDMTWVVLSDPIPGGASLLGSGLA 353 ++GYRIT+ + A+EQKQ G+Y+RGDVLRVTLDIDAQ +MTWVV+SDP+PGGA+LLGSGL Sbjct: 1792 AAGYRITRKVEAIEQKQAGVYTRGDVLRVTLDIDAQAEMTWVVVSDPVPGGATLLGSGLG 1851 Query: 354 RDSAITRSGEDDGRSRGSAWLAYEERSFEGYRAYYERMPKGRSSISYTVRLNNAGEFR-- 411 RDSAI + +D R RG WLAYEERSFE YRAYYE MPKGR+SISYTVRLNNAGEF Sbjct: 1852 RDSAIAQGKQDAPRERGMGWLAYEERSFEAYRAYYEAMPKGRTSISYTVRLNNAGEFNMP 1911 Query: 412 -XRVEAMYAPEMFGEIPNARMSIKA 435 RVEAMYAPEMFGE+PN ++ +KA Sbjct: 1912 PTRVEAMYAPEMFGEVPNGKLVVKA 1936 >ref|YP_004753991.1| alpha-2-macroglobulin domain-containing protein [Collimonas fungivorans Ter331] gb|AEK63168.1| alpha-2-macroglobulin domain protein [Collimonas fungivorans Ter331] Length = 1961 Score = 646 bits (1667), Expect = 0.0, Method: Compositional matrix adjust. Identities = 311/445 (69%), Positives = 371/445 (83%), Gaps = 14/445 (3%) Query: 1 LPTYLDADGLAYYFPPSESNARRGSDTLTAYLLAASNEAAYALPLQSRDKMLDGLAAFVE 60 LPTYLD+DGLAYY+PP+E NARRGSDTLTAYLLA + E+ +A+P QSRDKMLDGLAAFVE Sbjct: 1520 LPTYLDSDGLAYYYPPNEGNARRGSDTLTAYLLAVTQESGFAIPQQSRDKMLDGLAAFVE 1579 Query: 61 GKLTRDFWSPKKDLDVRKLAALEALSRYHRVQPSMLGSVQINPNLWPTSAVLDWIAILQR 120 GK+TRDFWSP+KDLDVRKLAALEALSRY RV+P+ML S+QI+PNLWPTSAVLDWI ILQR Sbjct: 1580 GKITRDFWSPQKDLDVRKLAALEALSRYDRVRPAMLDSIQISPNLWPTSAVLDWINILQR 1639 Query: 121 VTTIPERDKRLAEADQIIRSRLNYQGTRMGFSDEAGDYWWWLMASGDSNANRLVLTMLNN 180 V++IPER KR AEA+QIIRSRLNYQ TRMGFS E+ DYWWWLMAS D+NANRLVLTML N Sbjct: 1640 VSSIPERAKRQAEAEQIIRSRLNYQSTRMGFSTESSDYWWWLMASADTNANRLVLTMLEN 1699 Query: 181 PAWREDMPKLISGAIQRQQRGHWNTTTANVWGSLALEKFSRAFEAEKVTGTTRV-----V 235 PAW+EDMPKLI+GAIQRQ RGHW+TTTAN+WGSLAL+KF+R FE+EKVTG T+ Sbjct: 1700 PAWKEDMPKLINGAIQRQSRGHWSTTTANLWGSLALQKFARKFESEKVTGVTKASLEQGA 1759 Query: 236 SDAGNNAHAW--SGAGVSGGKLQLPW-GTPAPGTLKFSQEGSGKPWITMQSLAAVPLKSP 292 + +G+ +++W + A GKLQL W + AP LK S GSG PW+T+QSLAAV LK P Sbjct: 1760 AVSGSQSYSWPAAAAATEPGKLQLSWPASAAPADLKLSHAGSGAPWVTVQSLAAVVLKKP 1819 Query: 293 FSSGYRITKTIVAVEQKQQGIYSRGDVLRVTLDIDAQTDMTWVVLSDPIPGGASLLGSGL 352 FSSGYRI+K IV VEQK++G Y+RGD++R+ L++DAQTDMTWVV++DPIP GA+LLGSGL Sbjct: 1820 FSSGYRISKNIVPVEQKEKGKYTRGDIVRIDLEVDAQTDMTWVVVTDPIPAGATLLGSGL 1879 Query: 353 ARDSAITRSGEDDGRSRGSAWLAYEERSFEGYRAYYERMPKGRSSISYTVRLNNAGEFR- 411 RDSAI S E R SAW+AYEERSFE +R+YYE +PKG+ +++YT R+NN GEF Sbjct: 1880 GRDSAIATSSERPSR---SAWVAYEERSFEAFRSYYEYVPKGKFTMTYTFRINNPGEFSL 1936 Query: 412 --XRVEAMYAPEMFGEIPNARMSIK 434 RVEAMYAPEMFGE PN + ++K Sbjct: 1937 PPTRVEAMYAPEMFGESPNQKWTVK 1961 >ref|YP_002943272.1| alpha-2-macroglobulin [Variovorax paradoxus S110] gb|ACS18006.1| alpha-2-macroglobulin domain protein [Variovorax paradoxus S110] Length = 2012 Score = 529 bits (1363), Expect = e-148, Method: Compositional matrix adjust. Identities = 269/473 (56%), Positives = 340/473 (71%), Gaps = 41/473 (8%) Query: 1 LPTYLDADGLAYYFPPSESNARRGSDTLTAYLLAASNEAA-----YALPLQSRDKMLDGL 55 LPTYLD+DGLA YFPP + + RGSD LT+YLLAA++EAA +ALP +R M GL Sbjct: 1541 LPTYLDSDGLASYFPPRDGESNRGSDILTSYLLAATHEAAALNPAFALPDAARAPMESGL 1600 Query: 56 AAFVEGKLTRDFWSPKKDLDVRKLAALEALSRYHRVQPSMLGSVQINPNLWPTSAVLDWI 115 AFVEG++ R+FWSP+KDLDVRKLAALEALSRY + SMLGS+ I PN WPTSAV+DW+ Sbjct: 1601 IAFVEGRIQREFWSPRKDLDVRKLAALEALSRYGTAKGSMLGSITIAPNQWPTSAVIDWL 1660 Query: 116 AILQRVTTIPERDKRLAEADQIIRSRLNYQGTRMGFSDEAGDYWWWLMASGDSNANRLVL 175 IL+RV +P+R +RL EA+ ++++RL+YQGT++ FS E DYWWWLM +GD N RL+L Sbjct: 1661 NILKRVQDVPQRQQRLEEANNVLKARLSYQGTKLVFSTEQDDYWWWLMTNGDVNTARLLL 1720 Query: 176 TMLNNPAWREDMPKLISGAIQRQQRGHWNTTTANVWGSLALEKFSRAFEAEKVTGTT--- 232 +++++PAW++D+ KL +G I RQQ G W+TTTAN+WG LALEKFS+ FE+ V G T Sbjct: 1721 SVMDDPAWKDDIGKLANGFIGRQQNGAWHTTTANLWGGLALEKFSKVFESTPVAGVTAAT 1780 Query: 233 --------------RV-VSDAGN--NAHAWSGAGVSGGKLQ-----LPW-----GTPAPG 265 RV +DA N + GA S G L+ LPW GTP Sbjct: 1781 LGAVKAQVDWRSVERVKTTDAAGAPNQSTFFGAPASPGNLKNNSMFLPWAASPAGTPVRD 1840 Query: 266 TLKFSQEGSGKPWITMQSLAAVPLKSPFSSGYRITKTIVAVEQKQQGIYSRGDVLRVTLD 325 TL +Q+GSGKPW+TMQS+AAV LK+PF++GY I KTI VEQ G Y+RGDVLRVT++ Sbjct: 1841 TLLVTQQGSGKPWLTMQSIAAVQLKAPFAAGYAIKKTITPVEQATAGKYTRGDVLRVTVE 1900 Query: 326 IDAQTDMTWVVLSDPIPGGASLLGSGLARDSAITRSGEDDGRSRGSAWLAYEERSFEGYR 385 ++A TDMTWVV+SDPIPGGA++LG+GL RDS I GE R G+ WLA+EERSFE +R Sbjct: 1901 VNASTDMTWVVVSDPIPGGATILGTGLGRDSQIATQGE---RKSGAGWLAFEERSFEAFR 1957 Query: 386 AYYERMPKGRSSISYTVRLNNAGEFR---XRVEAMYAPEMFGEIPNARMSIKA 435 +YYE +PKG I YTVRLNN G+F RVEAMYAPEMFGE PNAR+ ++A Sbjct: 1958 SYYEYLPKGVVKIEYTVRLNNVGDFALPPSRVEAMYAPEMFGETPNARVKVEA 2010 >ref|YP_983330.1| alpha-2-macroglobulin domain-containing protein [Polaromonas naphthalenivorans CJ2] gb|ABM38409.1| alpha-2-macroglobulin domain protein [Polaromonas naphthalenivorans CJ2] Length = 1999 Score = 529 bits (1362), Expect = e-148, Method: Compositional matrix adjust. Identities = 270/474 (56%), Positives = 338/474 (71%), Gaps = 39/474 (8%) Query: 1 LPTYLDADGLAYYFPPSESNARRGSDTLTAYLLAASNEA-----AYALPLQSRDKMLDGL 55 LP YLD+DGLA YFPP + +A RGS TLTAYLLAA++EA A+ALP ++R M GL Sbjct: 1524 LPGYLDSDGLASYFPPRDGDAARGSATLTAYLLAATHEASGINPAFALPDEARAPMERGL 1583 Query: 56 AAFVEGKLTRDFWSPKKDLDVRKLAALEALSRYHRVQPSMLGSVQINPNLWPTSAVLDWI 115 AFVEG++ RDFWSP+KDLDVRKLAALEALSRY + Q M GS+ I PN WPT AV+DW+ Sbjct: 1584 IAFVEGRIQRDFWSPRKDLDVRKLAALEALSRYGKAQGRMTGSITIAPNQWPTHAVIDWL 1643 Query: 116 AILQRVTTIPERDKRLAEADQIIRSRLNYQGTRMGFSDEAGDYWWWLMASGDSNANRLVL 175 IL+RV +P+R++RL EA QI+RSRL+YQGTR+ FS E DYWWWLM +GD N RL+L Sbjct: 1644 NILKRVADVPQREERLLEASQILRSRLSYQGTRLVFSTEKDDYWWWLMVNGDVNTARLML 1703 Query: 176 TMLNNPAWREDMPKLISGAIQRQQRGHWNTTTANVWGSLALEKFSRAFEAEKVTGTTRVV 235 ++++ AW++DM +L +G I RQQ+G W TTTAN+WG LAL+KFS FEA V G T+ Sbjct: 1704 AVMDDAAWKDDMARLANGFIGRQQQGAWRTTTANLWGGLALDKFSAKFEAVPVAGVTQAA 1763 Query: 236 -----------------------SDAGNNAH--AWSGAGVSGGKLQ-----LPWGTPAPG 265 +DA AH ++ GA + G L+ LPWG+PA Sbjct: 1764 LASAAATAKTASVDWRKVGRITPADASGAAHQTSFFGAPAAPGMLRNNTLFLPWGSPAKD 1823 Query: 266 TLKFSQEGSGKPWITMQSLAAVPLKSPFSSGYRITKTIVAVEQKQQGIYSRGDVLRVTLD 325 TL + EGSGKPW+T+QSLAAV LK+PFS+GY+I+KTI VEQ +G Y+RGDVLR+TL+ Sbjct: 1824 TLTVTHEGSGKPWLTLQSLAAVQLKAPFSAGYQISKTITPVEQAVKGQYTRGDVLRITLE 1883 Query: 326 IDAQTDMTWVVLSDPIPGGASLLGSGLARDSAITRSGEDDGRSR-GSAWLAYEERSFEGY 384 I+A DMTWV ++DPIPGGA++LGSGL RDS I SGE S G W A+EERSFE + Sbjct: 1884 INASADMTWVAITDPIPGGATILGSGLGRDSQIATSGEKKAASMGGGGWPAFEERSFEAF 1943 Query: 385 RAYYERMPKGRSSISYTVRLNNAGEFR---XRVEAMYAPEMFGEIPNARMSIKA 435 R+YYE +PKG + YTVRLNNAG F RVEA+YAPEMFGE PNAR+ ++A Sbjct: 1944 RSYYEYLPKGVLKLDYTVRLNNAGSFALPPSRVEALYAPEMFGEAPNARVKVEA 1997 >ref|YP_004153808.1| alpha-2-macroglobulin domain-containing protein [Variovorax paradoxus EPS] gb|ADU35697.1| alpha-2-macroglobulin domain protein [Variovorax paradoxus EPS] Length = 2008 Score = 520 bits (1339), Expect = e-145, Method: Compositional matrix adjust. Identities = 265/473 (56%), Positives = 335/473 (70%), Gaps = 41/473 (8%) Query: 1 LPTYLDADGLAYYFPPSESNARRGSDTLTAYLLAASNEAA-----YALPLQSRDKMLDGL 55 LPTYLD DGLA YFPP + A RGSD LT+YLLAA+NEAA +AL R M GL Sbjct: 1537 LPTYLDGDGLANYFPPRDGEANRGSDILTSYLLAATNEAAQLNPAFALADDVRAPMESGL 1596 Query: 56 AAFVEGKLTRDFWSPKKDLDVRKLAALEALSRYHRVQPSMLGSVQINPNLWPTSAVLDWI 115 AFVEG++TR+FWSP+KDLDVRKLAALEALSRY + + SMLGS+ I PN WPTSAV+DW+ Sbjct: 1597 IAFVEGRITREFWSPRKDLDVRKLAALEALSRYGKAKGSMLGSITIAPNQWPTSAVIDWL 1656 Query: 116 AILQRVTTIPERDKRLAEADQIIRSRLNYQGTRMGFSDEAGDYWWWLMASGDSNANRLVL 175 IL+RV +P+R +RL EA+ ++++RL+YQGT++ FS E DYWWWLM +GD N RL+L Sbjct: 1657 NILKRVNDVPQRQQRLDEANNVLKARLSYQGTKLIFSTEQDDYWWWLMTNGDVNTARLLL 1716 Query: 176 TMLNNPAWREDMPKLISGAIQRQQRGHWNTTTANVWGSLALEKFSRAFEAEKVTGTT--- 232 +++++P+W++D+ KL +G I RQQ G W+TTTAN+WG LALEKFS+ FE+ V G T Sbjct: 1717 SVMDDPSWKDDIGKLANGFIGRQQNGAWHTTTANLWGGLALEKFSKVFESTPVAGVTAAT 1776 Query: 233 --------------RV-VSDAGN--NAHAWSGAGVSGGKLQ-----LPW-----GTPAPG 265 RV SDA N + GA S G L+ LPW G P Sbjct: 1777 LGAVKAQVNWSQVERVKTSDAAGAPNQTTFFGAPASPGNLKNNTMFLPWAASPSGAPVRD 1836 Query: 266 TLKFSQEGSGKPWITMQSLAAVPLKSPFSSGYRITKTIVAVEQKQQGIYSRGDVLRVTLD 325 TL +Q+G+GKPW+T+QS+AAV LK+PF++GY I KTI VEQ G Y+RGDVLRVTL+ Sbjct: 1837 TLLVTQQGTGKPWLTLQSIAAVQLKAPFAAGYAIKKTITPVEQATAGKYTRGDVLRVTLE 1896 Query: 326 IDAQTDMTWVVLSDPIPGGASLLGSGLARDSAITRSGEDDGRSRGSAWLAYEERSFEGYR 385 ++A DMTWVV+SDPIPGGA++LG GL RDS I GE + G+ W A+EERSFE +R Sbjct: 1897 VNASADMTWVVISDPIPGGATILGGGLGRDSQIATQGE---KKSGAGWAAFEERSFEAFR 1953 Query: 386 AYYERMPKGRSSISYTVRLNNAGEFR---XRVEAMYAPEMFGEIPNARMSIKA 435 +YYE +PKG + YTVRLNN G+F RVEAMYAPEMFGE PNAR+ ++A Sbjct: 1954 SYYEYLPKGVVKMEYTVRLNNVGDFALPPSRVEAMYAPEMFGEAPNARVKVEA 2006