BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0145.p (576 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003773667.1| proline/betaine transporter protein [Herbasp... 596 e-168 ref|YP_841023.1| major facilitator superfamily transporter MHS f... 587 e-165 ref|YP_295112.1| major facilitator transporter [Ralstonia eutrop... 586 e-165 ref|YP_004681701.1| citrate-proton symporter CitA [Cupriavidus n... 578 e-163 ref|YP_558002.1| major facilitator superfamily metabolite/H(+) s... 535 e-150 >ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] gb|ADJ61759.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 596 bits (1536), Expect = e-168, Method: Compositional matrix adjust. Identities = 303/345 (87%), Positives = 322/345 (93%) Query: 226 DAGGRKAALSLTILLMAAGTTLIGIAPTYEQIGLFAPLIIVVARLLQGFSAGGEMGSATA 285 D GRKAALS+TILLMA GTT+IG+APTYE IGLFAPLIIVVARLLQGFSAGGEMGSATA Sbjct: 87 DRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSAGGEMGSATA 146 Query: 286 FLTEYAPVRERAFYSSWIQASIGVAVLLGAAVGSYVTSSLSPEALASWGWRLPFLLGIII 345 FLTEYAP RERA+YSSWIQASIGVAVLLGAAVG++VT+SL ALASWGWRLPFLLGIII Sbjct: 147 FLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGWRLPFLLGIII 206 Query: 346 GPVGYYIRHHLDETPAFLEAKDKTDSPLKEIIRNYPRETTASFSMVILWTVCTYVLLFYM 405 GPVGYYIRHHL+ETP FLE KDKTDSPLKE+IRN+PRET+ASFSMVILWTVCTYVLLFYM Sbjct: 207 GPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSMVILWTVCTYVLLFYM 266 Query: 406 PTYSIKVLKLPQSTGFIAGMVGGLCIMVFAPVVGRIADRYGRRGLLSGAALLILLLAYPM 465 PTYS+KVLKLPQ+TGFIAGMVGGLCIMV AP+VGR+ADR GRR LLSG+A LIL+LAYPM Sbjct: 267 PTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLLSGSAALILMLAYPM 326 Query: 466 FAYINHAPGLGSLLVFQLVFGILIATYTGPILAAFSELFPAKVLSTGLSVAYNLAVTIFG 525 FAYIN AP L +LL+FQLVFG LIA YTGPILAAFSELFPAKVLSTGLSVAYNLAVTIFG Sbjct: 327 FAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGLSVAYNLAVTIFG 386 Query: 526 GFASFIITGLIAATGSTMAPAFYVMIAAAISLVGTRFVREPAYLQ 570 GFASF IT LIAATGSTMAPAFYVMIAAAIS VGTRFVREP YLQ Sbjct: 387 GFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVREPQYLQ 431 >ref|YP_841023.1| major facilitator superfamily transporter MHS family protein [Ralstonia eutropha H16] emb|CAJ96293.1| MFS transporter, MHS family [Ralstonia eutropha H16] Length = 441 Score = 587 bits (1514), Expect = e-165, Method: Compositional matrix adjust. Identities = 293/346 (84%), Positives = 320/346 (92%) Query: 226 DAGGRKAALSLTILLMAAGTTLIGIAPTYEQIGLFAPLIIVVARLLQGFSAGGEMGSATA 285 D GRKAALSLTILLMA GTTLIGIAPTY+QIGLFAPL+IVVARL+QGFSAGGEMG ATA Sbjct: 95 DRVGRKAALSLTILLMALGTTLIGIAPTYDQIGLFAPLLIVVARLMQGFSAGGEMGGATA 154 Query: 286 FLTEYAPVRERAFYSSWIQASIGVAVLLGAAVGSYVTSSLSPEALASWGWRLPFLLGIII 345 FLTEYAP R+RA+YSSWIQASIGVAVLLGAAVG++VTSSLS EAL SWGWRLPFLLGI+I Sbjct: 155 FLTEYAPARQRAYYSSWIQASIGVAVLLGAAVGTFVTSSLSTEALNSWGWRLPFLLGIVI 214 Query: 346 GPVGYYIRHHLDETPAFLEAKDKTDSPLKEIIRNYPRETTASFSMVILWTVCTYVLLFYM 405 GPVGYYIRHHLDETP F + ++ DSPLKEI++ YPRET ASFSMVILWTVCTYVLLFYM Sbjct: 215 GPVGYYIRHHLDETPTFRDNAERADSPLKEIVQAYPRETLASFSMVILWTVCTYVLLFYM 274 Query: 406 PTYSIKVLKLPQSTGFIAGMVGGLCIMVFAPVVGRIADRYGRRGLLSGAALLILLLAYPM 465 PTY++KVLK+PQ+ GFIAGM GG IMVFAP+VG +ADR GRR LLSG+ALLIL+LA+PM Sbjct: 275 PTYAVKVLKVPQADGFIAGMAGGSAIMVFAPLVGLLADRIGRRVLLSGSALLILVLAWPM 334 Query: 466 FAYINHAPGLGSLLVFQLVFGILIATYTGPILAAFSELFPAKVLSTGLSVAYNLAVTIFG 525 FAYINHAPGL SLLVFQLVFG+LIATYTGPILAAFSELFPA+VLSTGLSVAYN AVTIFG Sbjct: 335 FAYINHAPGLASLLVFQLVFGVLIATYTGPILAAFSELFPARVLSTGLSVAYNFAVTIFG 394 Query: 526 GFASFIITGLIAATGSTMAPAFYVMIAAAISLVGTRFVREPAYLQR 571 GFASFIIT LIA TGS+MAPA YVMIAAAISLVGTRFVREP+YLQ+ Sbjct: 395 GFASFIITWLIATTGSSMAPAIYVMIAAAISLVGTRFVREPSYLQQ 440 >ref|YP_295112.1| major facilitator transporter [Ralstonia eutropha JMP134] gb|AAZ60268.1| General substrate transporter:Major facilitator superfamily MFS_1 [Ralstonia eutropha JMP134] Length = 447 Score = 586 bits (1511), Expect = e-165, Method: Compositional matrix adjust. Identities = 291/346 (84%), Positives = 316/346 (91%) Query: 226 DAGGRKAALSLTILLMAAGTTLIGIAPTYEQIGLFAPLIIVVARLLQGFSAGGEMGSATA 285 D GRKAALS+TILLMA GTTLIGIAPTYEQ+G+FAPL+IVVARLLQGFSAGGEMG ATA Sbjct: 101 DRVGRKAALSMTILLMALGTTLIGIAPTYEQMGIFAPLLIVVARLLQGFSAGGEMGGATA 160 Query: 286 FLTEYAPVRERAFYSSWIQASIGVAVLLGAAVGSYVTSSLSPEALASWGWRLPFLLGIII 345 FLTEYAP RERA+YSSWIQASIGVAVLLGAAVG++VT SLSPEAL SWGWRLPFLLGI+I Sbjct: 161 FLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTRSLSPEALNSWGWRLPFLLGIVI 220 Query: 346 GPVGYYIRHHLDETPAFLEAKDKTDSPLKEIIRNYPRETTASFSMVILWTVCTYVLLFYM 405 GPVGYYIRHHLDETP F E ++ DSPLKE+ + YPRET ASFSMVILWTVCTYVLLFYM Sbjct: 221 GPVGYYIRHHLDETPTFRETDERADSPLKEVFQAYPRETLASFSMVILWTVCTYVLLFYM 280 Query: 406 PTYSIKVLKLPQSTGFIAGMVGGLCIMVFAPVVGRIADRYGRRGLLSGAALLILLLAYPM 465 PTY++KVL +PQS GF+AGMVGG IM+FAP+VGR+ADR GRR LLSG+ALLIL+LA+PM Sbjct: 281 PTYAVKVLNVPQSDGFVAGMVGGCTIMLFAPLVGRLADRIGRRALLSGSALLILVLAWPM 340 Query: 466 FAYINHAPGLGSLLVFQLVFGILIATYTGPILAAFSELFPAKVLSTGLSVAYNLAVTIFG 525 FAYINH PGL SLLVFQLVFGILIA YTGPILAAFSELFPA+VLSTGLSVAYN AVTIFG Sbjct: 341 FAYINHVPGLSSLLVFQLVFGILIAAYTGPILAAFSELFPARVLSTGLSVAYNFAVTIFG 400 Query: 526 GFASFIITGLIAATGSTMAPAFYVMIAAAISLVGTRFVREPAYLQR 571 GFASFIIT LIA TGS+MAPA YVMIAAAISL GTR VREPAYLQ+ Sbjct: 401 GFASFIITWLIATTGSSMAPAIYVMIAAAISLAGTRLVREPAYLQQ 446 >ref|YP_004681701.1| citrate-proton symporter CitA [Cupriavidus necator N-1] gb|AEI80469.1| citrate-proton symporter CitA [Cupriavidus necator N-1] Length = 441 Score = 578 bits (1491), Expect = e-163, Method: Compositional matrix adjust. Identities = 286/346 (82%), Positives = 317/346 (91%) Query: 226 DAGGRKAALSLTILLMAAGTTLIGIAPTYEQIGLFAPLIIVVARLLQGFSAGGEMGSATA 285 D GRKAALSLTILLMA GTTLIGIAPTY+Q+G+ APL+IV+ARL+QGFSAGGEMG ATA Sbjct: 95 DRVGRKAALSLTILLMALGTTLIGIAPTYDQVGILAPLLIVIARLMQGFSAGGEMGGATA 154 Query: 286 FLTEYAPVRERAFYSSWIQASIGVAVLLGAAVGSYVTSSLSPEALASWGWRLPFLLGIII 345 FLTEYAP R+RA+YSSWIQASIGVAVLLGAAVG++VTSSLS EAL SWGWRLPFLLGI+I Sbjct: 155 FLTEYAPARQRAYYSSWIQASIGVAVLLGAAVGTFVTSSLSTEALNSWGWRLPFLLGIVI 214 Query: 346 GPVGYYIRHHLDETPAFLEAKDKTDSPLKEIIRNYPRETTASFSMVILWTVCTYVLLFYM 405 GPVGYYIRHHLDETP F + ++ DSPLKEI++ YPRET ASFSMVILWTVCTYVLLFYM Sbjct: 215 GPVGYYIRHHLDETPTFRDNAERADSPLKEIVQAYPRETLASFSMVILWTVCTYVLLFYM 274 Query: 406 PTYSIKVLKLPQSTGFIAGMVGGLCIMVFAPVVGRIADRYGRRGLLSGAALLILLLAYPM 465 PTY++KVLK+PQ+ GFIAGM GG IMVFAP+VG +ADR GRR LLSG+ALLIL+LA+PM Sbjct: 275 PTYAVKVLKVPQADGFIAGMAGGSAIMVFAPLVGLLADRIGRRVLLSGSALLILVLAWPM 334 Query: 466 FAYINHAPGLGSLLVFQLVFGILIATYTGPILAAFSELFPAKVLSTGLSVAYNLAVTIFG 525 F +INH PGL SLLVFQLVFG+LIATYTGPILAAFSELFPA+VLSTGLSVAYN AVTIFG Sbjct: 335 FTFINHVPGLSSLLVFQLVFGVLIATYTGPILAAFSELFPARVLSTGLSVAYNFAVTIFG 394 Query: 526 GFASFIITGLIAATGSTMAPAFYVMIAAAISLVGTRFVREPAYLQR 571 GFASFIIT LIA TGS+MAPA YVMIAAAISLVGTRFVREP+YLQ+ Sbjct: 395 GFASFIITWLIATTGSSMAPAIYVMIAAAISLVGTRFVREPSYLQQ 440 >ref|YP_558002.1| major facilitator superfamily metabolite/H(+) symporter [Burkholderia xenovorans LB400] gb|ABE29950.1| Major facilitator superfamily (MFS) metabolite/H+ symporter [Burkholderia xenovorans LB400] Length = 435 Score = 535 bits (1379), Expect = e-150, Method: Compositional matrix adjust. Identities = 267/343 (77%), Positives = 302/343 (88%), Gaps = 2/343 (0%) Query: 226 DAGGRKAALSLTILLMAAGTTLIGIAPTYEQIGLFAPLIIVVARLLQGFSAGGEMGSATA 285 D GRKAALSLTILLMAAGT LIG+APTY+QIGL+AP++IVVARLLQGFSAGGEMGSATA Sbjct: 88 DKVGRKAALSLTILLMAAGTALIGLAPTYDQIGLWAPVLIVVARLLQGFSAGGEMGSATA 147 Query: 286 FLTEYAPVRERAFYSSWIQASIGVAVLLGAAVGSYVTSSLSPEALASWGWRLPFLLGIII 345 FLTEYAPV +RAFYSSWIQ+SIG AVLLGAAVG++VT+SLS +AL SWGWR+PFL+GI+I Sbjct: 148 FLTEYAPVEKRAFYSSWIQSSIGFAVLLGAAVGTFVTASLSADALHSWGWRMPFLIGILI 207 Query: 346 GPVGYYIRHHLDETPAF--LEAKDKTDSPLKEIIRNYPRETTASFSMVILWTVCTYVLLF 403 GPVGY+IR +DETPAF + + + DSPL E+ R +PRET ASFSMVILWTVCTYVLLF Sbjct: 208 GPVGYFIRSRMDETPAFSAVAEEARNDSPLAEVFRRFPRETFASFSMVILWTVCTYVLLF 267 Query: 404 YMPTYSIKVLKLPQSTGFIAGMVGGLCIMVFAPVVGRIADRYGRRGLLSGAALLILLLAY 463 YMPTYS++ L LPQSTGF+AGM+GGL IM F+PVVG +ADR+GRR LSGAA+LILLLA+ Sbjct: 268 YMPTYSVRTLHLPQSTGFLAGMLGGLMIMCFSPVVGSLADRFGRRRFLSGAAVLILLLAW 327 Query: 464 PMFAYINHAPGLGSLLVFQLVFGILIATYTGPILAAFSELFPAKVLSTGLSVAYNLAVTI 523 PMF YIN PGL SL+VFQ VFG+LIA YTGPILAAFSELFP KVLSTGLSVAYN AVTI Sbjct: 328 PMFTYINRVPGLASLMVFQGVFGVLIAAYTGPILAAFSELFPTKVLSTGLSVAYNFAVTI 387 Query: 524 FGGFASFIITGLIAATGSTMAPAFYVMIAAAISLVGTRFVREP 566 FGGFA F IT LIA+TGS MAPA YVMIAAA+SL GT FVR+P Sbjct: 388 FGGFAPFFITWLIASTGSNMAPAIYVMIAAAVSLAGTFFVRDP 430