BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0126.p (241 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_02905138.1| nitroreductase [Burkholderia ambifaria MEX-5]... 334 7e-90 ref|YP_293138.1| nitroreductase [Ralstonia eutropha JMP134] >gi|... 333 1e-89 ref|YP_001861059.1| nitroreductase [Burkholderia phymatum STM815... 333 2e-89 ref|YP_004687716.1| nitroreductase [Cupriavidus necator N-1] >gi... 332 3e-89 ref|YP_547538.1| nitroreductase [Polaromonas sp. JS666] >gi|9169... 331 6e-89 >ref|ZP_02905138.1| nitroreductase [Burkholderia ambifaria MEX-5] gb|EDT43702.1| nitroreductase [Burkholderia ambifaria MEX-5] Length = 254 Score = 334 bits (856), Expect = 7e-90, Method: Compositional matrix adjust. Identities = 152/240 (63%), Positives = 191/240 (79%) Query: 1 MDRAGLPPAIGEDMLRAAVEWAIASRRSIRAFLPTEVPREQIENILNIARFAATGVNLQP 60 MDR PP D + AV+W + +RRS+RAFLPT VPREQIE IL++ARF+ATGVN+QP Sbjct: 14 MDRLSPPPLNSFDDVARAVDWTLVTRRSVRAFLPTPVPREQIEAILDVARFSATGVNIQP 73 Query: 61 WRVHVVTGDARERLCSAIHKVDNDPALAAQHQDEWDYYPTNWISPYIDRRRDVGWNLYGL 120 W VHVVTG + RL +AI+++++DP +A + +DYYP WI+PY+DRRR VGWNLYGL Sbjct: 74 WHVHVVTGATKSRLSAAINEINDDPGRSAGLDEPYDYYPRKWIAPYVDRRRAVGWNLYGL 133 Query: 121 LGIQKGDKERMHAQHGRNYSFFDAPVGLIFTVNRVMRQGSLLDCGMFMQNIMVAARTHGL 180 LGI+KGDKERMH QHGRNY+FFDAPVGL FTV+RVMR+GSLLD GMF+Q++M+AAR GL Sbjct: 134 LGIEKGDKERMHVQHGRNYNFFDAPVGLFFTVDRVMREGSLLDTGMFLQSVMIAARARGL 193 Query: 181 STCPQAAFMKYHKIISAELEFTADEMLVCGMSLGHAAPEKIENTLITKREGVASFTKFHN 240 TCPQAAF K+H++I+ EL ++MLVCGMSLG+A IENTL+T+R V FT F+N Sbjct: 194 DTCPQAAFNKFHRVIANELAIPDNQMLVCGMSLGYADRGCIENTLVTERSPVRDFTTFYN 253 >ref|YP_293138.1| nitroreductase [Ralstonia eutropha JMP134] gb|AAZ65281.1| Nitroreductase [Ralstonia eutropha JMP134] Length = 237 Score = 333 bits (853), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 153/235 (65%), Positives = 182/235 (77%) Query: 7 PPAIGEDMLRAAVEWAIASRRSIRAFLPTEVPREQIENILNIARFAATGVNLQPWRVHVV 66 PP +R AVEWAI +RRS+RAFLP V + +E ILN ARFAATGVN+QPW+VHV+ Sbjct: 3 PPPDELLAVRQAVEWAITTRRSVRAFLPKPVTADTVEAILNTARFAATGVNIQPWKVHVL 62 Query: 67 TGDARERLCSAIHKVDNDPALAAQHQDEWDYYPTNWISPYIDRRRDVGWNLYGLLGIQKG 126 G A++R+C+AI ++D+DPA+A H DEW YYP W SPY+DRRR VGW LY LLGI+KG Sbjct: 63 MGAAKDRVCAAIKQIDDDPAVAVMHADEWAYYPKEWTSPYVDRRRAVGWRLYSLLGIEKG 122 Query: 127 DKERMHAQHGRNYSFFDAPVGLIFTVNRVMRQGSLLDCGMFMQNIMVAARTHGLSTCPQA 186 DK RM QHGRNY FF APVGL FTV+R+M +GSLLD GMF+QNIMV ART+GLSTCPQA Sbjct: 123 DKARMQVQHGRNYQFFGAPVGLFFTVDRLMSEGSLLDYGMFLQNIMVVARTYGLSTCPQA 182 Query: 187 AFMKYHKIISAELEFTADEMLVCGMSLGHAAPEKIENTLITKREGVASFTKFHNR 241 AFMKYH+II EL A E V GMSLG+A E+IENTL ++RE ASFT FH + Sbjct: 183 AFMKYHRIIEQELGLGAHEKFVVGMSLGYADEERIENTLASEREPAASFTTFHEK 237 >ref|YP_001861059.1| nitroreductase [Burkholderia phymatum STM815] gb|ACC74013.1| nitroreductase [Burkholderia phymatum STM815] Length = 247 Score = 333 bits (853), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 149/239 (62%), Positives = 191/239 (79%) Query: 1 MDRAGLPPAIGEDMLRAAVEWAIASRRSIRAFLPTEVPREQIENILNIARFAATGVNLQP 60 M+R PP +R A+ WAI++RRS+RAFL V E+IE+IL++ARF+A+GVN+QP Sbjct: 1 MNRLSPPPFASLQDVRRAINWAISTRRSVRAFLSKPVSHEEIESILDVARFSASGVNMQP 60 Query: 61 WRVHVVTGDARERLCSAIHKVDNDPALAAQHQDEWDYYPTNWISPYIDRRRDVGWNLYGL 120 W VHVVTG+ + RL +AI +VDNDP + +D +DYYP W++PY+DRRR VGW+LYGL Sbjct: 61 WHVHVVTGEVKTRLSAAIKEVDNDPVRSLGLEDPYDYYPREWVTPYLDRRRKVGWSLYGL 120 Query: 121 LGIQKGDKERMHAQHGRNYSFFDAPVGLIFTVNRVMRQGSLLDCGMFMQNIMVAARTHGL 180 LGI+KGDKERMH QHGRNYSFFDAPVGL FT++RVM GSLLD GMF+QN+M+AAR HGL Sbjct: 121 LGIEKGDKERMHFQHGRNYSFFDAPVGLFFTIDRVMGVGSLLDTGMFLQNVMIAARGHGL 180 Query: 181 STCPQAAFMKYHKIISAELEFTADEMLVCGMSLGHAAPEKIENTLITKREGVASFTKFH 239 TCPQAAF+K+H++IS+ L+ ++MLVCGMSLG+A +ENTLIT+RE V +FT FH Sbjct: 181 HTCPQAAFLKFHRVISSVLQIPENQMLVCGMSLGYADENSLENTLITEREPVRAFTTFH 239 >ref|YP_004687716.1| nitroreductase [Cupriavidus necator N-1] gb|AEI81678.1| nitroreductase [Cupriavidus necator N-1] Length = 239 Score = 332 bits (851), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 151/240 (62%), Positives = 188/240 (78%), Gaps = 1/240 (0%) Query: 1 MDRAGLPPAIGEDMLRAAVEWAIASRRSIRAFLPTEVPREQIENILNIARFAATGVNLQP 60 M+R PPA + +R AVEWAI +RRS+RAF P V E + IL++ARFAATGVN+QP Sbjct: 1 MNRVAPPPAELQS-VREAVEWAIVTRRSVRAFRPQPVDVETVHKILDVARFAATGVNIQP 59 Query: 61 WRVHVVTGDARERLCSAIHKVDNDPALAAQHQDEWDYYPTNWISPYIDRRRDVGWNLYGL 120 W+VHVVTG AR+R+C+AI +VD+DPA + H DEW YYP W++PY+DRRR+VGW LYGL Sbjct: 60 WQVHVVTGQARDRICAAIKQVDDDPAQSGAHSDEWAYYPDKWVAPYLDRRREVGWRLYGL 119 Query: 121 LGIQKGDKERMHAQHGRNYSFFDAPVGLIFTVNRVMRQGSLLDCGMFMQNIMVAARTHGL 180 LGI+KG+K RMHAQHGRNY FFDAPVGL FTV+R+M +GSL+D GMF+QN+MV ART+GL Sbjct: 120 LGIEKGNKARMHAQHGRNYQFFDAPVGLFFTVDRLMNEGSLIDYGMFLQNLMVVARTYGL 179 Query: 181 STCPQAAFMKYHKIISAELEFTADEMLVCGMSLGHAAPEKIENTLITKREGVASFTKFHN 240 STCPQAAFMKYH II EL A+E + GMSLG+A E++EN L++ R +SFT H Sbjct: 180 STCPQAAFMKYHAIIRRELSLGAEEKFLVGMSLGYADDERVENQLVSDRAPASSFTVVHT 239 >ref|YP_547538.1| nitroreductase [Polaromonas sp. JS666] gb|ABE42640.1| nitroreductase [Polaromonas sp. JS666] Length = 249 Score = 331 bits (848), Expect = 6e-89, Method: Compositional matrix adjust. Identities = 150/238 (63%), Positives = 185/238 (77%) Query: 3 RAGLPPAIGEDMLRAAVEWAIASRRSIRAFLPTEVPREQIENILNIARFAATGVNLQPWR 62 R PP+I D LR AV+WA+ +RRS RAFLPT VPR +E IL++AR+ A+GVN QPW Sbjct: 4 RLASPPSISTDSLRGAVDWALVTRRSTRAFLPTPVPRTDVEAILDVARYCASGVNTQPWH 63 Query: 63 VHVVTGDARERLCSAIHKVDNDPALAAQHQDEWDYYPTNWISPYIDRRRDVGWNLYGLLG 122 VHV+ G A++RL AI ++ +DP+LAA + +DYYP WISPYIDRRR VGW+LYGLLG Sbjct: 64 VHVLMGAAKQRLSEAIMQIHDDPSLAATLDEPYDYYPQEWISPYIDRRRKVGWDLYGLLG 123 Query: 123 IQKGDKERMHAQHGRNYSFFDAPVGLIFTVNRVMRQGSLLDCGMFMQNIMVAARTHGLST 182 IQK DKERMHAQHG NYSFF APVGL+FT++R M +GSL+D GMF+Q+IMVAAR GL T Sbjct: 124 IQKSDKERMHAQHGCNYSFFGAPVGLMFTIDRGMGRGSLIDYGMFLQSIMVAARARGLDT 183 Query: 183 CPQAAFMKYHKIISAELEFTADEMLVCGMSLGHAAPEKIENTLITKREGVASFTKFHN 240 CPQAAF +H++IS EL +M VCGMSLG+A P +IEN+L+T RE V+SFT FH Sbjct: 184 CPQAAFTTFHRVISRELAIPEQQMFVCGMSLGYADPSRIENSLVTDREVVSSFTTFHE 241