BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0734.p (434 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777711.1| tRNA modification (thiophene/furan oxidation... 724 0.0 ref|YP_004755091.1| GTPase and tRNA-U34 5-formylation enzyme Trm... 718 0.0 ref|YP_001355383.1| tRNA modification GTPase TrmE [Janthinobacte... 667 0.0 ref|YP_001101690.1| tRNA modification GTPase TrmE [Herminiimonas... 667 0.0 ref|ZP_08274923.1| GTPase and tRNA-U34 5-formylation enzyme TrmE... 556 e-156 >ref|YP_003777711.1| tRNA modification (thiophene/furan oxidation) GTPase [Herbaspirillum seropedicae SmR1] gb|ADJ65803.1| tRNA modification (thiophene/furan oxidation) GTPase protein [Herbaspirillum seropedicae SmR1] Length = 479 Score = 724 bits (1870), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/468 (79%), Positives = 404/468 (86%), Gaps = 36/468 (7%) Query: 1 MTLDTSPIAAIATAPGRGGIGVVRLSGKNLWPVVQAICGKDESSLAPRHASYLPFKRADG 60 MT D+SPIAAIATAPGRGGIGVVRLSGKNL PV+QA+CG+ +SLAPRHA+YLPF DG Sbjct: 14 MTFDSSPIAAIATAPGRGGIGVVRLSGKNLAPVIQAVCGR--ASLAPRHATYLPFLNEDG 71 Query: 61 SLIDQGLAIYFKAPHSYTGEDVLELQGHGGPVVMQMLLARCLEAGVDIGLRL-------- 112 S IDQGLAI+F APHSYTGEDVLELQGHGGPVVMQMLLARC++AG +IGLRL Sbjct: 72 SAIDQGLAIHFPAPHSYTGEDVLELQGHGGPVVMQMLLARCVQAGSEIGLRLAEPGEFTQ 131 Query: 113 --------------------------AXKSASESLSGAFSKVIHDLVNKVTNLRMLVEAT 146 A KSASESLSGAFSKVIH+LV++VT LRMLVEAT Sbjct: 132 RAFLNDKIDLAQAEAVADLIEATTEAAAKSASESLSGAFSKVIHELVDQVTRLRMLVEAT 191 Query: 147 LDFPEEEIDFLEQSDARGQLAAIRATLDDVFMHASQGALLRDGLNVVLAGKPNVGKSSLL 206 LDFPEEEIDFL+QSDARGQLA IR+TL++VF HA+QGALLRDGLN VLAGKPNVGKSSLL Sbjct: 192 LDFPEEEIDFLKQSDARGQLATIRSTLEEVFKHAAQGALLRDGLNAVLAGKPNVGKSSLL 251 Query: 207 NVLAGSDVAIVTPIAGTTRDKVTETIQIEGMPLNIIDTAGIREAEEDGGGPDEVERIGIE 266 NVLAGSDVAIVTPIAGTTRDKVT+TIQIEGMPLNIIDTAGIREA +DG GPDEVERIGIE Sbjct: 252 NVLAGSDVAIVTPIAGTTRDKVTQTIQIEGMPLNIIDTAGIREAGDDGSGPDEVERIGIE 311 Query: 267 RTWGEVAKADVILHMLDADRGPTLEDEKITERFPDGVPVIRIWNKIDRSGHKPAVDKMSD 326 RTW EVAKADVILHMLDADRGPTLEDEKIT RFPDGVP+IRIWNKIDRSGHK AVDKM D Sbjct: 312 RTWAEVAKADVILHMLDADRGPTLEDEKITSRFPDGVPIIRIWNKIDRSGHKAAVDKMPD 371 Query: 327 ATHIYLSATDQVGIDLLRGELLRIAGWQQTVESRYLARERHLIALKAARDYLESAAMHAS 386 ATH+YLSA++ G+DLLR ELLRIAGWQQTVESRYLARERHL+ALKAA ++L AA HA+ Sbjct: 372 ATHVYLSASEGQGVDLLRAELLRIAGWQQTVESRYLARERHLLALKAADEHLAHAAAHAA 431 Query: 387 VDNQASDKSLDLFAEELRLAQERLSSITGKFTPDDLLGVIFSRFCIGK 434 +DN+A + +LDLFAEELRLAQERLSSITGKFTPDDLLGVIFSRFCIGK Sbjct: 432 LDNEAGEHALDLFAEELRLAQERLSSITGKFTPDDLLGVIFSRFCIGK 479 >ref|YP_004755091.1| GTPase and tRNA-U34 5-formylation enzyme TrmE [Collimonas fungivorans Ter331] gb|AEK64268.1| GTPase and tRNA-U34 5-formylation enzyme TrmE [Collimonas fungivorans Ter331] Length = 465 Score = 718 bits (1853), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/468 (76%), Positives = 394/468 (84%), Gaps = 37/468 (7%) Query: 1 MTLDTSPIAAIATAPGRGGIGVVRLSGKNLWPVVQAICGKDESSLAPRHASYLPFKRADG 60 M+ D+SPIAAIATAPGRGGIGVVR+SGK L +++A+CG ES L PRHA+YLPFK ADG Sbjct: 1 MSFDSSPIAAIATAPGRGGIGVVRISGKQLGSLIEAVCGIAESKLTPRHATYLPFKNADG 60 Query: 61 SLIDQGLAIYFKAPHSYTGEDVLELQGHGGPVVMQMLLARCLEAGVDIGLRL-------- 112 S+IDQGLAIYFKAPHSYTGEDVLELQGHGGPVV+QMLL+RCL AG DIGLR+ Sbjct: 61 SVIDQGLAIYFKAPHSYTGEDVLELQGHGGPVVLQMLLSRCLAAGQDIGLRMAEPGEFTQ 120 Query: 113 --------------------------AXKSASESLSGAFSKVIHDLVNKVTNLRMLVEAT 146 A KSASESLSGAFSKVIH LV +VTNLRMLVEAT Sbjct: 121 RAFINDKLDLAQAEAVADLIEASTEAAAKSASESLSGAFSKVIHALVGQVTNLRMLVEAT 180 Query: 147 LDFPEEEIDFLEQSDARGQLAAIRATLDDVFMHASQGALLRDGLNVVLAGKPNVGKSSLL 206 LDFPEEEIDFLE+SDARGQLA IR+TLD+VF A+QGALLRDGLN+VLAG+PNVGKSSLL Sbjct: 181 LDFPEEEIDFLEKSDARGQLATIRSTLDNVFSQAAQGALLRDGLNIVLAGQPNVGKSSLL 240 Query: 207 NVLAGSDVAIVTPIAGTTRDKVTETIQIEGMPLNIIDTAGIREAEEDGGGPDEVERIGIE 266 NVLAGSDVAIVTPIAGTTRDKVTETIQ+EG+PLNIIDTAGIREAE+ DEVERIGIE Sbjct: 241 NVLAGSDVAIVTPIAGTTRDKVTETIQLEGIPLNIIDTAGIREAED---ANDEVERIGIE 297 Query: 267 RTWGEVAKADVILHMLDADRGPTLEDEKITERFPDGVPVIRIWNKIDRSGHKPAVDKMSD 326 RTW VA ADVILHMLDA+ GP+L DEKI RFP+ P+IRIWNKID+SGHKPAVD M+D Sbjct: 298 RTWAAVANADVILHMLDANHGPSLADEKIVARFPNSAPIIRIWNKIDQSGHKPAVDVMND 357 Query: 327 ATHIYLSATDQVGIDLLRGELLRIAGWQQTVESRYLARERHLIALKAARDYLESAAMHAS 386 ATHIYLSATD +GIDLLR ELLRIAGWQQT ESRYLARERHLIALKAARD+LE A HAS Sbjct: 358 ATHIYLSATDHLGIDLLRTELLRIAGWQQTGESRYLARERHLIALKAARDHLEIADQHAS 417 Query: 387 VDNQASDKSLDLFAEELRLAQERLSSITGKFTPDDLLGVIFSRFCIGK 434 +DN+ +D+SLDLFAEELRLAQ+RLSSITG FTPDDLLGVIFSRFCIGK Sbjct: 418 LDNRVNDQSLDLFAEELRLAQDRLSSITGAFTPDDLLGVIFSRFCIGK 465 >ref|YP_001355383.1| tRNA modification GTPase TrmE [Janthinobacterium sp. Marseille] sp|A6T4D6.1|MNME_JANMA RecName: Full=tRNA modification GTPase MnmE gb|ABR89668.1| tRNA modification GTPase [Janthinobacterium sp. Marseille] Length = 465 Score = 667 bits (1722), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/468 (70%), Positives = 377/468 (80%), Gaps = 37/468 (7%) Query: 1 MTLDTSPIAAIATAPGRGGIGVVRLSGKNLWPVVQAICGKDESSLAPRHASYLPFKRADG 60 M D+SPIAAIATAPGRGGIGVVR+SGKN+ +++A+C + L PRHA++ F ADG Sbjct: 1 MNFDSSPIAAIATAPGRGGIGVVRVSGKNISSIIEAVCATKGAELQPRHATFTNFVNADG 60 Query: 61 SLIDQGLAIYFKAPHSYTGEDVLELQGHGGPVVMQMLLARCLEAGVDIGLRLA------- 113 S+IDQGLAIYFKAPHSYTGEDVLELQGHGGP+V+QMLL RCLEAG DIGLR+A Sbjct: 61 SVIDQGLAIYFKAPHSYTGEDVLELQGHGGPIVLQMLLTRCLEAGTDIGLRMAQPGEFTH 120 Query: 114 ---------------------------XKSASESLSGAFSKVIHDLVNKVTNLRMLVEAT 146 KSAS+SLSGAFSK I DLV+K+TNLRMLVEAT Sbjct: 121 RAFLNDKLDLAQAEGVIDLIEASTEAAAKSASQSLSGAFSKTIQDLVDKITNLRMLVEAT 180 Query: 147 LDFPEEEIDFLEQSDARGQLAAIRATLDDVFMHASQGALLRDGLNVVLAGKPNVGKSSLL 206 LDFPEEEIDFLE+SDARGQL IR L VF ASQGALLRDGLN+VLAG+PNVGKSSLL Sbjct: 181 LDFPEEEIDFLEKSDARGQLNGIREALQAVFTQASQGALLRDGLNIVLAGQPNVGKSSLL 240 Query: 207 NVLAGSDVAIVTPIAGTTRDKVTETIQIEGMPLNIIDTAGIREAEEDGGGPDEVERIGIE 266 N LAGSDVAIVT IAGTTRDKV ETIQIEG+P+N+IDTAGIR+A + DEVERIGIE Sbjct: 241 NALAGSDVAIVTAIAGTTRDKVIETIQIEGIPVNVIDTAGIRDASD---ATDEVERIGIE 297 Query: 267 RTWGEVAKADVILHMLDADRGPTLEDEKITERFPDGVPVIRIWNKIDRSGHKPAVDKMSD 326 RTW V ADVI+HMLDA+RGPT DE+I ERFP+ +PV+RIWNKID SGH+PA+D+M D Sbjct: 298 RTWAAVKTADVIIHMLDANRGPTRADEQIVERFPENIPVMRIWNKIDLSGHRPAIDRMPD 357 Query: 327 ATHIYLSATDQVGIDLLRGELLRIAGWQQTVESRYLARERHLIALKAARDYLESAAMHAS 386 +THIY+SATD G+DLLRGELLR+ GWQQT ES YLARERHL+ALK+A D+LE AA HA+ Sbjct: 358 STHIYVSATDLQGMDLLRGELLRLIGWQQTGESLYLARERHLVALKSAHDHLEMAAQHAA 417 Query: 387 VDNQASDKSLDLFAEELRLAQERLSSITGKFTPDDLLGVIFSRFCIGK 434 D++A+D +LDLFAEELRLAQERLSSITG+FT DDLLGVIFSRFCIGK Sbjct: 418 HDSEATDPALDLFAEELRLAQERLSSITGEFTSDDLLGVIFSRFCIGK 465 >ref|YP_001101690.1| tRNA modification GTPase TrmE [Herminiimonas arsenicoxydans] sp|A4GAN2.1|MNME_HERAR RecName: Full=tRNA modification GTPase MnmE emb|CAL63569.1| tRNA modification GTPase TrmE [Herminiimonas arsenicoxydans] Length = 466 Score = 667 bits (1721), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/469 (71%), Positives = 380/469 (81%), Gaps = 38/469 (8%) Query: 1 MTLDTSPIAAIATAPGRGGIGVVRLSGKNLWPVVQAICGKDE-SSLAPRHASYLPFKRAD 59 MT D+SPIAAIATAPGRGGIGVVR+SGK+L V++A+CG D+ SSL RHA+YL F +D Sbjct: 1 MTFDSSPIAAIATAPGRGGIGVVRISGKDLSSVMRAVCGADKGSSLQARHATYLDFVNSD 60 Query: 60 GSLIDQGLAIYFKAPHSYTGEDVLELQGHGGPVVMQMLLARCLEAGVDIGLRL------- 112 GS+IDQGLAIYFKAPHSYTGEDVLELQGHGGPVV+QMLL RCLEAG DIGLR+ Sbjct: 61 GSVIDQGLAIYFKAPHSYTGEDVLELQGHGGPVVLQMLLTRCLEAGADIGLRMAEPGEFT 120 Query: 113 ---------------------------AXKSASESLSGAFSKVIHDLVNKVTNLRMLVEA 145 A KSA++SLSGAFSK I +LV+K+TNLRMLVEA Sbjct: 121 HRAFLNDKLDLAQAEGVIDLIEASTEAAAKSATQSLSGAFSKTIQELVDKITNLRMLVEA 180 Query: 146 TLDFPEEEIDFLEQSDARGQLAAIRATLDDVFMHASQGALLRDGLNVVLAGKPNVGKSSL 205 TLDFPEEEIDFLE+SDARGQL IR L VF A+QGALLRDGLN+VLAG+PNVGKSSL Sbjct: 181 TLDFPEEEIDFLEKSDARGQLTNIREALQRVFSQAAQGALLRDGLNIVLAGQPNVGKSSL 240 Query: 206 LNVLAGSDVAIVTPIAGTTRDKVTETIQIEGMPLNIIDTAGIREAEEDGGGPDEVERIGI 265 LN LAGSDVAIVTPIAGTTRDKV ETIQIEG+P+N+IDTAGIR+A + G DEVERIGI Sbjct: 241 LNALAGSDVAIVTPIAGTTRDKVIETIQIEGIPVNVIDTAGIRDAADAG---DEVERIGI 297 Query: 266 ERTWGEVAKADVILHMLDADRGPTLEDEKITERFPDGVPVIRIWNKIDRSGHKPAVDKMS 325 ERTW V ADVI+HMLDA RGPT DE+ITERFP VPV+R+WNKID SGH+PA+D+M Sbjct: 298 ERTWAAVQTADVIVHMLDASRGPTRADEQITERFPANVPVMRVWNKIDLSGHRPAIDRMP 357 Query: 326 DATHIYLSATDQVGIDLLRGELLRIAGWQQTVESRYLARERHLIALKAARDYLESAAMHA 385 DATHIYLSAT +G+DLLR ELL + GWQQT ES YLARERHL+ALK+A +LE AA HA Sbjct: 358 DATHIYLSATGLLGMDLLRTELLHLVGWQQTGESLYLARERHLVALKSAHSHLEMAAQHA 417 Query: 386 SVDNQASDKSLDLFAEELRLAQERLSSITGKFTPDDLLGVIFSRFCIGK 434 + DN+A+D +LDLFAEELRLAQERLSSITG+FT DDLLGVIFSRFCIGK Sbjct: 418 AHDNEATDPALDLFAEELRLAQERLSSITGEFTSDDLLGVIFSRFCIGK 466 >ref|ZP_08274923.1| GTPase and tRNA-U34 5-formylation enzyme TrmE [Oxalobacteraceae bacterium IMCC9480] gb|EGF31597.1| GTPase and tRNA-U34 5-formylation enzyme TrmE [Oxalobacteraceae bacterium IMCC9480] Length = 433 Score = 556 bits (1433), Expect = e-156, Method: Compositional matrix adjust. Identities = 293/425 (68%), Positives = 335/425 (78%), Gaps = 41/425 (9%) Query: 44 SLAPRHASYLPFKRADGSLIDQGLAIYFKAPHSYTGEDVLELQGHGGPVVMQMLLARCLE 103 +L R A+Y F ADGS IDQGLA+YFKAPHSYTGEDVLELQGHGGPVVMQ+LLARCL+ Sbjct: 16 TLQARRATYTRFVDADGSTIDQGLALYFKAPHSYTGEDVLELQGHGGPVVMQLLLARCLQ 75 Query: 104 AGVDIGLRL----------------------------------AXKSASESLSGAFSKVI 129 AG I LRL A +SAS+SLSG FS +I Sbjct: 76 AGSHIDLRLAQPGEFTQRAFLNDKLDLAQAEAVADLIDASTEAAARSASQSLSGVFSGII 135 Query: 130 HDLVNKVTNLRMLVEATLDFPEEEIDFLEQSDARGQLAAIRATLDDVFMHASQGALLRDG 189 LV +VT+LRMLVEATLDFPEEEIDFLE+SDARG+LAAIR TL VF ++QGALLRDG Sbjct: 136 RTLVEQVTHLRMLVEATLDFPEEEIDFLEKSDARGKLAAIRDTLQKVFRQSAQGALLRDG 195 Query: 190 LNVVLAGKPNVGKSSLLNVLAGSDVAIVTPIAGTTRDKVTETIQIEGMPLNIIDTAGIRE 249 LN+VLAG+PNVGKSSLLN LAG+D+AIVTPIAGTTRDKVTETIQIEG+PLNIIDTAGIR Sbjct: 196 LNIVLAGQPNVGKSSLLNALAGADIAIVTPIAGTTRDKVTETIQIEGIPLNIIDTAGIR- 254 Query: 250 AEEDGGGPDEVERIGIERTWGEVAKADVILHMLDADRGPTLEDEKITERFPDGVPVIRIW 309 D D+VERIGIERTW VA+ADVI+H+LDA RGPTL DE I RFP +PV+R+W Sbjct: 255 --YDAEAIDDVERIGIERTWAAVAQADVIIHLLDACRGPTLADESIVARFPANIPVMRVW 312 Query: 310 NKIDRSGHKPAVDKMSDATHIYLSATDQVGIDLLRGELLRIAGWQQTVESRYLARERHLI 369 NKID SGHKPA+D++ DATHIYLSATDQ+G++LLR ELLR+AGWQQT ES YLARERHL+ Sbjct: 313 NKIDLSGHKPAIDRLVDATHIYLSATDQLGMELLREELLRLAGWQQTGESLYLARERHLV 372 Query: 370 ALKAARDYLESAAMHASVDNQASDKSLDLFAEELRLAQERLSSITGKFTPDDLLGVIFSR 429 AL AA+ +L+ AA HA+++ D +LDLFAEELRLAQE+LSSITG FT DDLLGVIFSR Sbjct: 373 ALAAAQTHLQFAAEHAALN----DHALDLFAEELRLAQEQLSSITGAFTSDDLLGVIFSR 428 Query: 430 FCIGK 434 FCIGK Sbjct: 429 FCIGK 433