BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0577.p (428 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777610.1| type III effector HopAN1 protein [Herbaspiri... 770 0.0 ref|ZP_02883896.1| type III effector Hrp-dependent protein [Burk... 660 0.0 ref|ZP_06844144.1| type III effector Hrp-dependent outer protein... 654 0.0 ref|YP_001889636.1| Hrp-dependent type III effector protein [Bur... 654 0.0 ref|YP_003909377.1| Hrp-dependent type III effector domain-conta... 649 0.0 >ref|YP_003777610.1| type III effector HopAN1 protein [Herbaspirillum seropedicae SmR1] gb|ADJ65702.1| type III effector HopAN1 protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 770 bits (1988), Expect = 0.0, Method: Compositional matrix adjust. Identities = 378/427 (88%), Positives = 402/427 (94%) Query: 1 MSQAKPLLGCIADDFTGATDLANMLVRGGMRTVQTIGIPEQLPAVAADALVIALKSRTIP 60 MS ++PLLGCIADDFTGATDLANMLVRGGMRTVQTIG+P ++PAVAADALVIALKSRT+P Sbjct: 1 MSTSRPLLGCIADDFTGATDLANMLVRGGMRTVQTIGVPAEMPAVAADALVIALKSRTVP 60 Query: 61 AAEAVEQSLAALRWLQQQGCTQFFFKYCSTFDSTDAGNIGQVTDALLKELGADFTIACPA 120 AAEA+ QSLAALRWLQQQGCTQFFFKYCSTFDSTDAGNIG VTDAL+KELG DFTIACPA Sbjct: 61 AAEAIAQSLAALRWLQQQGCTQFFFKYCSTFDSTDAGNIGPVTDALMKELGVDFTIACPA 120 Query: 121 FPENGRTIYRGYLFVADGLLNESGMENHPLTPMTDANLVRVLQRQTESKVGLVRYDTVAK 180 FPENGRTIYRGYLFVAD LLNESGMENHPLTPMTDANLVRVLQRQTESKVGLVRYDTVA+ Sbjct: 121 FPENGRTIYRGYLFVADALLNESGMENHPLTPMTDANLVRVLQRQTESKVGLVRYDTVAR 180 Query: 181 DAAAVTERFTALRTDGVKMAIADAVSDRDLYVLGEACAELKLVTGGSGIALGLPENFRRA 240 AAA ERFTALR++GVKMAIADAVSD+DL+ LGEACAELKL+TGGSG+ALGLPENFRRA Sbjct: 181 GAAATQERFTALRSEGVKMAIADAVSDKDLFTLGEACAELKLITGGSGVALGLPENFRRA 240 Query: 241 GLLHASAEAAQLPQVGGLSAVLAGSASKATNAQVAEWTARHPAWRIDPLALARGEAVVAQ 300 GLL A EAAQLP+V GLS VLAGSASKATNAQVAEW A+ PA+RIDPLALARGE VVAQ Sbjct: 241 GLLTAVGEAAQLPKVDGLSVVLAGSASKATNAQVAEWKAKRPAFRIDPLALARGEDVVAQ 300 Query: 301 ALAFADEHISKEAVLIYATATPDEVKAVQKELGVAKAGHLVEQALADIARGLRERGVRRF 360 ALAFADE I E VLIYATATPDEVKAVQKELGVAKAGHLVEQALA IA GLR+RGVR+F Sbjct: 301 ALAFADERIKNEPVLIYATATPDEVKAVQKELGVAKAGHLVEQALASIAAGLRQRGVRQF 360 Query: 361 VVAGGETSGAVVQALDVRALRIGPQIDPGVPATATLDDQPLALALKSGNFGSVDFFEKAL 420 VVAGGETSGAVVQAL+VRALRIGPQIDPGVPATATLDDQP+ALALKSGNFGSVDFFEKAL Sbjct: 361 VVAGGETSGAVVQALEVRALRIGPQIDPGVPATATLDDQPVALALKSGNFGSVDFFEKAL 420 Query: 421 RFLAGET 427 R+L G+T Sbjct: 421 RYLDGKT 427 >ref|ZP_02883896.1| type III effector Hrp-dependent protein [Burkholderia graminis C4D1M] gb|EDT10556.1| type III effector Hrp-dependent protein [Burkholderia graminis C4D1M] Length = 436 Score = 660 bits (1704), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/427 (79%), Positives = 364/427 (85%), Gaps = 6/427 (1%) Query: 5 KPLLGCIADDFTGATDLANMLVRGGMRTVQTIGIPEQLPAVAADALVIALKSRTIPAAEA 64 + LLGCIADDFTGATDLANMLVRGGMRTVQTIGIP+ +AADA+V+ALKSRTIPAA+A Sbjct: 7 RALLGCIADDFTGATDLANMLVRGGMRTVQTIGIPQDGETIAADAIVVALKSRTIPAADA 66 Query: 65 VEQSLAALRWLQQQGCTQFFFKYCSTFDSTDAGNIGQVTDALLKELGAD-----FTIACP 119 V QSLAAL WL+ QGC QF FKYCSTFDSTDAGNIG VTDALL L AD FTIACP Sbjct: 67 VAQSLAALDWLRAQGCRQFVFKYCSTFDSTDAGNIGPVTDALLDALCADDKASAFTIACP 126 Query: 120 AFPENGRTIYRGYLFVADGLLNESGMENHPLTPMTDANLVRVLQRQTESKVGLVRYDTVA 179 AFPENGRTI+RG+LFV D LL+ESGMENHPLTPM DANLVRVLQRQT+S+VGLVRYD VA Sbjct: 127 AFPENGRTIFRGHLFVGDALLSESGMENHPLTPMRDANLVRVLQRQTQSQVGLVRYDAVA 186 Query: 180 KDAAAVTERFTALRTDGVKMAIADAVSDRDLYVLGEACAELKLVTGGSGIALGLPENFRR 239 K AAAV E F ALR G +MAIADAVSD DLYVLGEACA+LKL+TGGSGIALGLP NFRR Sbjct: 187 KGAAAVRESFDALRKQGARMAIADAVSDADLYVLGEACADLKLITGGSGIALGLPANFRR 246 Query: 240 AGLLHASAEAAQLPQVGGLSAVLAGSASKATNAQVAEWTARHPAWRIDPLALARGEAVVA 299 AGLL +AAQLP V GLSAVLAGSASKATNAQVA W PA+RIDPLA ARGEAVV Sbjct: 247 AGLLQPQDDAAQLPAVEGLSAVLAGSASKATNAQVAAWRDTRPAFRIDPLAAARGEAVVE 306 Query: 300 QALAFADEHISK-EAVLIYATATPDEVKAVQKELGVAKAGHLVEQALADIARGLRERGVR 358 QALAFA ++ K E +LIYATATPDEVKAVQ+ELGV KAGHLVE LA IARGL ERGVR Sbjct: 307 QALAFAQTYLDKGEPMLIYATATPDEVKAVQRELGVDKAGHLVEATLASIARGLYERGVR 366 Query: 359 RFVVAGGETSGAVVQALDVRALRIGPQIDPGVPATATLDDQPLALALKSGNFGSVDFFEK 418 +FVVAGGETSGAVVQALDV +LRIG QIDPGVPATAT QPLALALKSGNFG+ DFFEK Sbjct: 367 KFVVAGGETSGAVVQALDVHSLRIGAQIDPGVPATATTGSQPLALALKSGNFGATDFFEK 426 Query: 419 ALRFLAG 425 ALR L G Sbjct: 427 ALRHLNG 433 >ref|ZP_06844144.1| type III effector Hrp-dependent outer protein [Burkholderia sp. Ch1-1] gb|EFG68304.1| type III effector Hrp-dependent outer protein [Burkholderia sp. Ch1-1] Length = 436 Score = 654 bits (1686), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/430 (77%), Positives = 364/430 (84%), Gaps = 6/430 (1%) Query: 5 KPLLGCIADDFTGATDLANMLVRGGMRTVQTIGIPEQLPAVAADALVIALKSRTIPAAEA 64 + LLGCIADDFTGATDLANMLVRGGMRTVQTIG+P +AADA+V+ALKSRTIPAA+A Sbjct: 7 RALLGCIADDFTGATDLANMLVRGGMRTVQTIGVPASDETIAADAVVVALKSRTIPAADA 66 Query: 65 VEQSLAALRWLQQQGCTQFFFKYCSTFDSTDAGNIGQVTDALLKELGAD-----FTIACP 119 V QSLAAL WL+ QGC QFFFKYCSTFDSTDAGNIG VTDALL L AD FTIACP Sbjct: 67 VAQSLAALDWLRAQGCRQFFFKYCSTFDSTDAGNIGPVTDALLDALSADGNAGAFTIACP 126 Query: 120 AFPENGRTIYRGYLFVADGLLNESGMENHPLTPMTDANLVRVLQRQTESKVGLVRYDTVA 179 AFPENGRTI+RG+LFV D LLN SGMENHPLTPM DANLV VLQRQT SKVGLVRYD VA Sbjct: 127 AFPENGRTIFRGHLFVGDVLLNASGMENHPLTPMRDANLVSVLQRQTRSKVGLVRYDAVA 186 Query: 180 KDAAAVTERFTALRTDGVKMAIADAVSDRDLYVLGEACAELKLVTGGSGIALGLPENFRR 239 + +AV + F LR +GV+MAIADAVSD DLY+LGEACA+L L+TGGSGIALGLP NFRR Sbjct: 187 RGVSAVGKSFDTLRREGVRMAIADAVSDADLYILGEACADLSLITGGSGIALGLPANFRR 246 Query: 240 AGLLHASAEAAQLPQVGGLSAVLAGSASKATNAQVAEWTARHPAWRIDPLALARGEAVVA 299 AGLL A+AAQLP++ GLSAVLAGSASKATNAQVAEW A PA+R+DPLA ARGE VV Sbjct: 247 AGLLEDQADAAQLPRIEGLSAVLAGSASKATNAQVAEWRATRPAFRVDPLAAARGEKVVE 306 Query: 300 QALAFADEHISK-EAVLIYATATPDEVKAVQKELGVAKAGHLVEQALADIARGLRERGVR 358 QALAFA ++ K E +LIYATATPDEVKAVQ+ELGV +AGHLVE LA IARGLRE GVR Sbjct: 307 QALAFAQPYLDKGEPMLIYATATPDEVKAVQRELGVNEAGHLVEATLASIARGLRECGVR 366 Query: 359 RFVVAGGETSGAVVQALDVRALRIGPQIDPGVPATATLDDQPLALALKSGNFGSVDFFEK 418 +FVVAGGETSGAVVQALDV LRIG QIDPGVPATAT +PLALALKSGNFG+ DFFEK Sbjct: 367 KFVVAGGETSGAVVQALDVHTLRIGAQIDPGVPATATTGAEPLALALKSGNFGTTDFFEK 426 Query: 419 ALRFLAGETQ 428 ALR L G+ Q Sbjct: 427 ALRHLDGDMQ 436 >ref|YP_001889636.1| Hrp-dependent type III effector protein [Burkholderia phytofirmans PsJN] gb|ACD20265.1| type III effector Hrp-dependent outers [Burkholderia phytofirmans PsJN] Length = 436 Score = 654 bits (1686), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/430 (77%), Positives = 362/430 (84%), Gaps = 6/430 (1%) Query: 5 KPLLGCIADDFTGATDLANMLVRGGMRTVQTIGIPEQLPAVAADALVIALKSRTIPAAEA 64 + LLGCIADDFTGATDLANMLVRGGMRTVQTIG+P V ADALV+ALKSRTIPAA+A Sbjct: 7 RALLGCIADDFTGATDLANMLVRGGMRTVQTIGVPASNETVEADALVVALKSRTIPAADA 66 Query: 65 VEQSLAALRWLQQQGCTQFFFKYCSTFDSTDAGNIGQVTDALLKELGAD-----FTIACP 119 V QSLAAL WL+ QGC QFFFKYCSTFDSTDAGNIG VTDALL L A+ FTIACP Sbjct: 67 VAQSLAALDWLRAQGCRQFFFKYCSTFDSTDAGNIGPVTDALLDALSAEGKAGAFTIACP 126 Query: 120 AFPENGRTIYRGYLFVADGLLNESGMENHPLTPMTDANLVRVLQRQTESKVGLVRYDTVA 179 AFPENGRTI+RG+LFV D LLN SGMENHPLTPM DANLV VLQRQTESKVGLVRYD VA Sbjct: 127 AFPENGRTIFRGHLFVGDTLLNASGMENHPLTPMRDANLVSVLQRQTESKVGLVRYDAVA 186 Query: 180 KDAAAVTERFTALRTDGVKMAIADAVSDRDLYVLGEACAELKLVTGGSGIALGLPENFRR 239 + +AV + F LR +GV+MAIADAVSD DLYVLGEACA+L L+TGGSGIALGLP NFRR Sbjct: 187 QGVSAVRDAFDTLRNEGVRMAIADAVSDADLYVLGEACADLTLITGGSGIALGLPANFRR 246 Query: 240 AGLLHASAEAAQLPQVGGLSAVLAGSASKATNAQVAEWTARHPAWRIDPLALARGEAVVA 299 AGLL A+AAQLPQV GLSAVLAGSASKATN QVA+W + PA+R+DPLA ARGE VV Sbjct: 247 AGLLKEQADAAQLPQVKGLSAVLAGSASKATNEQVAQWRSARPAFRVDPLAAARGENVVE 306 Query: 300 QALAFADEHISK-EAVLIYATATPDEVKAVQKELGVAKAGHLVEQALADIARGLRERGVR 358 QALAFA ++ K E +LIYATATPDEVKAVQ++LGV +AGHLVE LA IARGL ERGVR Sbjct: 307 QALAFAQPYLDKAEPMLIYATATPDEVKAVQRQLGVNEAGHLVENTLASIARGLHERGVR 366 Query: 359 RFVVAGGETSGAVVQALDVRALRIGPQIDPGVPATATLDDQPLALALKSGNFGSVDFFEK 418 +FVVAGGETSGAVVQALDV LRIG QIDPGVPATAT +PLALALKSGNFG+ DFFEK Sbjct: 367 KFVVAGGETSGAVVQALDVHTLRIGAQIDPGVPATATTGAEPLALALKSGNFGTADFFEK 426 Query: 419 ALRFLAGETQ 428 ALR L G Q Sbjct: 427 ALRHLNGAAQ 436 >ref|YP_003909377.1| Hrp-dependent type III effector domain-containing protein [Burkholderia sp. CCGE1003] gb|ADN60086.1| type III effector Hrp-dependent outers domain protein [Burkholderia sp. CCGE1003] Length = 436 Score = 649 bits (1673), Expect = 0.0, Method: Compositional matrix adjust. Identities = 334/430 (77%), Positives = 362/430 (84%), Gaps = 6/430 (1%) Query: 5 KPLLGCIADDFTGATDLANMLVRGGMRTVQTIGIPEQLPAVAADALVIALKSRTIPAAEA 64 + LLGCIADDFTGATDLANMLVRGGMRTVQTIG+P Q + ADA+V+ALKSRTIPAAEA Sbjct: 7 RALLGCIADDFTGATDLANMLVRGGMRTVQTIGVPGQGEGIEADAIVVALKSRTIPAAEA 66 Query: 65 VEQSLAALRWLQQQGCTQFFFKYCSTFDSTDAGNIGQVTDALLKEL-GAD----FTIACP 119 V QSLAAL WL+ QGC QF FKYCSTFDSTDAGNIG VTDALL L G D FTIACP Sbjct: 67 VAQSLAALDWLRAQGCRQFVFKYCSTFDSTDAGNIGPVTDALLDALSGGDQASAFTIACP 126 Query: 120 AFPENGRTIYRGYLFVADGLLNESGMENHPLTPMTDANLVRVLQRQTESKVGLVRYDTVA 179 AFPENGRTI+RG+LFV D LLNESGMENHPLTPM DANLVRVLQRQT+++VGLVRYD VA Sbjct: 127 AFPENGRTIFRGHLFVGDALLNESGMENHPLTPMRDANLVRVLQRQTQAEVGLVRYDAVA 186 Query: 180 KDAAAVTERFTALRTDGVKMAIADAVSDRDLYVLGEACAELKLVTGGSGIALGLPENFRR 239 K AV + F ALR DGV+MAIADAV D DL+VLGEACA+L L+TGGSGIALGLP NFRR Sbjct: 187 KGVDAVRDSFDALREDGVRMAIADAVCDADLHVLGEACADLTLITGGSGIALGLPANFRR 246 Query: 240 AGLLHASAEAAQLPQVGGLSAVLAGSASKATNAQVAEWTARHPAWRIDPLALARGEAVVA 299 AGLL + AAQLP+V GLSAVLAGSASKATNAQVA W PA+R+DPLA ARGEAVV Sbjct: 247 AGLLESRENAAQLPRVEGLSAVLAGSASKATNAQVAAWRETRPAFRVDPLAAARGEAVVE 306 Query: 300 QALAFADEHISK-EAVLIYATATPDEVKAVQKELGVAKAGHLVEQALADIARGLRERGVR 358 QALAFA H+ K E +LIYATATPDEVKAVQ +LGV +AGHLVE LA IARGL ERGVR Sbjct: 307 QALAFAQTHLDKAEPMLIYATATPDEVKAVQNQLGVDRAGHLVEATLASIARGLYERGVR 366 Query: 359 RFVVAGGETSGAVVQALDVRALRIGPQIDPGVPATATLDDQPLALALKSGNFGSVDFFEK 418 +FVVAGGETSGAVVQAL+V +LRIG QIDPGVPATAT QPLALALKSGNFG+ DFFEK Sbjct: 367 KFVVAGGETSGAVVQALNVCSLRIGAQIDPGVPATATTGAQPLALALKSGNFGTTDFFEK 426 Query: 419 ALRFLAGETQ 428 ALR L G Q Sbjct: 427 ALRHLGGGAQ 436