BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0132.p (278 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003773889.1| carboxymethylenebutenolidase [Herbaspirillum... 445 e-123 ref|YP_001098627.1| putative carboxymethylenebutenolidase [Hermi... 409 e-112 ref|YP_001352014.1| carboxymethylenebutenolidase [Janthinobacter... 404 e-111 ref|YP_004751384.1| putative carboxymethylenebutenolidase (Diene... 389 e-106 ref|ZP_08275791.1| putative carboxymethylenebutenolidase (Dienel... 370 e-100 >ref|YP_003773889.1| carboxymethylenebutenolidase [Herbaspirillum seropedicae SmR1] gb|ADJ61981.1| carboxymethylenebutenolidase (dienelactone hydrolase) protein [Herbaspirillum seropedicae SmR1] Length = 294 Score = 445 bits (1145), Expect = e-123, Method: Compositional matrix adjust. Identities = 216/293 (73%), Positives = 242/293 (82%), Gaps = 16/293 (5%) Query: 1 MNDLKNDVDSLAGTTGLLDAGNRRTFI----------------XQTMIKTDTVGLTAGEV 44 MNDL+ND +SL G + L +RR+F+ QTMIKTDT GLTAGEV Sbjct: 1 MNDLRNDFNSLLGQSTLSGNADRRSFLKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEV 60 Query: 45 SVTVNGQKVPAYRAQPEGKTNLPVILVISEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 104 S+ VNGQ+VPAYRAQPE KT LPVI+V+SEIFGVHEHIADIARRFAKLGYLAIAPDFFVR Sbjct: 61 SIEVNGQRVPAYRAQPERKTGLPVIIVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 120 Query: 105 QGNPAAYPTIAELNKEIISKVPDEQVFGDLDAYVAWAGQNGGNPDKIGINGFCWGGRIVW 164 QGNP AY +IAEL K+IISKVPD QV GD+DAYV WAG++GG+ ++GINGFCWGGRIVW Sbjct: 121 QGNPMAYTSIAELQKDIISKVPDAQVMGDIDAYVKWAGEHGGDIRRLGINGFCWGGRIVW 180 Query: 165 LYSARNPKIKAGAAWYGRIVGEKSALQPQHPIDIAPTLKTPVLGLYGGKDGGIPVATVEQ 224 LYSA NPK+KAGAAWYGR+VGEKS L PQ PIDIAPTLKTPV+GLYGGKD GIPV+TVEQ Sbjct: 181 LYSAHNPKVKAGAAWYGRLVGEKSELFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQ 240 Query: 225 MKEALAKTGNKSEFVVFPNSGHAFNADYRPSYVEADAKEGWKRAVEWFKSHGV 277 MKEAL K GNKSEF V+ NSGHAFNADYRPSYVEADAK+ W + V +FK HGV Sbjct: 241 MKEALQKAGNKSEFHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQHGV 293 >ref|YP_001098627.1| putative carboxymethylenebutenolidase [Herminiimonas arsenicoxydans] emb|CAL60498.1| putative carboxymethylenebutenolidase [Herminiimonas arsenicoxydans] Length = 294 Score = 409 bits (1051), Expect = e-112, Method: Compositional matrix adjust. Identities = 200/293 (68%), Positives = 229/293 (78%), Gaps = 16/293 (5%) Query: 1 MNDLKNDVDSLAGTTGLLDAGNRRTFI----------------XQTMIKTDTVGLTAGEV 44 M +L+ D DSL T L D NRR FI Q +IKTD GLTAGE+ Sbjct: 1 MKNLQEDFDSLVAKTPLSDGVNRRGFIKTALGTGFAAAVLPVSAQHVIKTDDAGLTAGEI 60 Query: 45 SVTVNGQKVPAYRAQPEGKTNLPVILVISEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 104 +V VNGQ VP YRAQP+GKT+LPVILVISEIFGVHE+IAD+ARRFAKLGYLA+AP+ FVR Sbjct: 61 TVNVNGQAVPVYRAQPKGKTDLPVILVISEIFGVHEYIADVARRFAKLGYLALAPELFVR 120 Query: 105 QGNPAAYPTIAELNKEIISKVPDEQVFGDLDAYVAWAGQNGGNPDKIGINGFCWGGRIVW 164 QG+P +Y TIAEL KEIISKVPD QV DLDA VAWA NGGN K+GI GFCWGGRI W Sbjct: 121 QGDPGSYGTIAELQKEIISKVPDAQVMTDLDAVVAWAKANGGNTGKLGITGFCWGGRITW 180 Query: 165 LYSARNPKIKAGAAWYGRIVGEKSALQPQHPIDIAPTLKTPVLGLYGGKDGGIPVATVEQ 224 LY+A NP+IKAG AWYGR+VG+ + L P++PIDIA LK PVLGLYG +DGGIPV+TV Q Sbjct: 181 LYAAHNPQIKAGVAWYGRLVGDSTPLTPKNPIDIAAKLKAPVLGLYGAQDGGIPVSTVVQ 240 Query: 225 MKEALAKTGNKSEFVVFPNSGHAFNADYRPSYVEADAKEGWKRAVEWFKSHGV 277 MKEALAK +KSEF+VF NSGHAF+ADYR SYVEADAKEGW+ A+ WFK+HGV Sbjct: 241 MKEALAKGSSKSEFIVFKNSGHAFHADYRASYVEADAKEGWQHAITWFKTHGV 293 >ref|YP_001352014.1| carboxymethylenebutenolidase [Janthinobacterium sp. Marseille] gb|ABR88829.1| carboxymethylenebutenolidase [Janthinobacterium sp. Marseille] Length = 294 Score = 404 bits (1039), Expect = e-111, Method: Compositional matrix adjust. Identities = 193/293 (65%), Positives = 227/293 (77%), Gaps = 16/293 (5%) Query: 1 MNDLKNDVDSLAGTTGLLDAGNRRTFIXQTM----------------IKTDTVGLTAGEV 44 M++LK D DSL T + D +RR F+ + + TD+ GLT GE+ Sbjct: 1 MDNLKEDFDSLVAKTPISDGVDRRGFLKTALGSGFAAAVLPIAAHSAVTTDSKGLTVGEI 60 Query: 45 SVTVNGQKVPAYRAQPEGKTNLPVILVISEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 104 +V VNGQ VP YRAQP GKTNLPV+LVISEIFGVHE+IAD+ARRFAKLGYLA+AP+ FVR Sbjct: 61 TVNVNGQAVPVYRAQPAGKTNLPVVLVISEIFGVHEYIADVARRFAKLGYLALAPELFVR 120 Query: 105 QGNPAAYPTIAELNKEIISKVPDEQVFGDLDAYVAWAGQNGGNPDKIGINGFCWGGRIVW 164 QG+P +Y TIAEL KEIISKVPD QV DLDA VAWA NGGN DK+GI GFCWGGR+ W Sbjct: 121 QGDPGSYGTIAELQKEIISKVPDAQVMTDLDAVVAWAKANGGNTDKLGITGFCWGGRVTW 180 Query: 165 LYSARNPKIKAGAAWYGRIVGEKSALQPQHPIDIAPTLKTPVLGLYGGKDGGIPVATVEQ 224 LYSA NPKIKAG AWYGR+VG + L P +P+DIA LKTPVLGLYG +DGGIPV TV Q Sbjct: 181 LYSAHNPKIKAGVAWYGRLVGNNNELTPNNPVDIAAKLKTPVLGLYGAQDGGIPVTTVVQ 240 Query: 225 MKEALAKTGNKSEFVVFPNSGHAFNADYRPSYVEADAKEGWKRAVEWFKSHGV 277 M++AL+K +KSEFV+F NSGHAF+ADYR SYVEADAKEGW+R ++WFK+HGV Sbjct: 241 MQDALSKGKSKSEFVLFKNSGHAFHADYRSSYVEADAKEGWQRCIDWFKTHGV 293 >ref|YP_004751384.1| putative carboxymethylenebutenolidase (Dienelactone hydrolase) [Collimonas fungivorans Ter331] gb|AEK60561.1| putative carboxymethylenebutenolidase (Dienelactone hydrolase) [Collimonas fungivorans Ter331] Length = 305 Score = 389 bits (998), Expect = e-106, Method: Compositional matrix adjust. Identities = 193/303 (63%), Positives = 221/303 (72%), Gaps = 26/303 (8%) Query: 1 MNDLKNDVDSLAGTTGLLDAGNRRTFI----------------XQTMIKTDTVGLTAGEV 44 M +LK D DSL T L D +RRTF+ QT+IKTDT GLTAG + Sbjct: 2 MPNLKEDFDSLVAKTSLSDGVDRRTFMKTALGTGFAAAVLPVTAQTVIKTDTEGLTAGAL 61 Query: 45 SVTVNGQKVPAYRAQPEGKTNLPVILVISEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 104 + NG VP YRAQPEGKTNLPV+LV+SEIFGVHEHIADIARRFAKLGYLAIAP+ F R Sbjct: 62 DIEENGLSVPIYRAQPEGKTNLPVVLVVSEIFGVHEHIADIARRFAKLGYLAIAPNLFKR 121 Query: 105 QGNPAAYPTIAELNKEIISKVPDEQVFGDLDAYVAWAGQNGGNPDKIGINGFCWGGRIVW 164 QG+PAA+ +I EL +ISKVPD+QV GDLDA VAWA +NGGN +K+ + GFCWGGRI W Sbjct: 122 QGDPAAFASIPELYVNVISKVPDDQVLGDLDACVAWAKKNGGNVNKLAVTGFCWGGRITW 181 Query: 165 LYSARNPKIKAGAAWYGRIV----------GEKSALQPQHPIDIAPTLKTPVLGLYGGKD 214 LY++ NPK+KA AAWYG +V E AL P+ P+DIAPTLK PVLGLYG KD Sbjct: 182 LYASHNPKVKAAAAWYGSLVRGKDPEGKTEAEIKALTPKQPVDIAPTLKVPVLGLYGAKD 241 Query: 215 GGIPVATVEQMKEALAKTGNKSEFVVFPNSGHAFNADYRPSYVEADAKEGWKRAVEWFKS 274 GI V QMK ALAK +KSE VV+PNSGHAFNADYRPSYVEADAK+GWKR + WFK Sbjct: 242 QGITQDHVAQMKAALAKGSSKSEIVVYPNSGHAFNADYRPSYVEADAKDGWKRVLAWFKE 301 Query: 275 HGV 277 HGV Sbjct: 302 HGV 304 >ref|ZP_08275791.1| putative carboxymethylenebutenolidase (Dienelactone hydrolase) [Oxalobacteraceae bacterium IMCC9480] gb|EGF30734.1| putative carboxymethylenebutenolidase (Dienelactone hydrolase) [Oxalobacteraceae bacterium IMCC9480] Length = 295 Score = 370 bits (951), Expect = e-100, Method: Compositional matrix adjust. Identities = 178/289 (61%), Positives = 213/289 (73%), Gaps = 16/289 (5%) Query: 5 KNDVDSLAGTTGLLDAGNRRTFI----------------XQTMIKTDTVGLTAGEVSVTV 48 K D SL G + D +RRTF+ QTM+ TDT GLTAG +S+TV Sbjct: 6 KQDAASLIGASAFPDGFDRRTFMKVSIGTGFAAAVMPVSAQTMVMTDTDGLTAGTISLTV 65 Query: 49 NGQKVPAYRAQPEGKTNLPVILVISEIFGVHEHIADIARRFAKLGYLAIAPDFFVRQGNP 108 NG++VP YRAQP GKT LPVILVISEIFGVHEHIAD+ARRFAKLGYLA+APD FVRQG+ Sbjct: 66 NGEQVPVYRAQPAGKTKLPVILVISEIFGVHEHIADVARRFAKLGYLALAPDLFVRQGDA 125 Query: 109 AAYPTIAELNKEIISKVPDEQVFGDLDAYVAWAGQNGGNPDKIGINGFCWGGRIVWLYSA 168 A +I EL K ++SKVPD+QV DLDA VA+A NGG+ +++GI GFCWGGRI WLYSA Sbjct: 126 KAAISIPELIKNVVSKVPDQQVMTDLDAVVAYAKANGGDVERMGITGFCWGGRITWLYSA 185 Query: 169 RNPKIKAGAAWYGRIVGEKSALQPQHPIDIAPTLKTPVLGLYGGKDGGIPVATVEQMKEA 228 NP+++AG AWYG++ G KSAL P ++AP L PVLGLYG KD GI ++ MK A Sbjct: 186 HNPQVRAGVAWYGQLQGTKSALNPMSATELAPALTVPVLGLYGAKDTGISQDSIATMKAA 245 Query: 229 LAKTGNKSEFVVFPNSGHAFNADYRPSYVEADAKEGWKRAVEWFKSHGV 277 LA ++SEFVVFPNSGHAF+ADYR SYVEADAKEGWK+ + WF +HGV Sbjct: 246 LATGKSQSEFVVFPNSGHAFHADYRASYVEADAKEGWKKCLAWFAAHGV 294