BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0035.p (453 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value F1W0G1.1 F1W0G1_9BURK Transmembrane protein drug efflux protein 751 0.0 D8NBL9.1 D8NBL9_RALSO Putative multidrug efflux transporter AcrB... 632 e-179 C6BIS4.1 C6BIS4_RALP1 Acriflavin resistance protein 628 e-178 E2T3M2.1 E2T3M2_9RALS RND efflux transporter, hydrophobe/amphiph... 628 e-178 Q8XYV2.1 Q8XYV2_RALSO Probable transmembrane drug efflux protein 627 e-177 >F1W0G1.1 F1W0G1_9BURK Transmembrane protein drug efflux protein Length = 1029 Score = 751 bits (1939), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/450 (81%), Positives = 400/450 (88%) Query: 1 VLVTPGDNLQVRVSGAFHSPKELEEMQLRANGTTFRLGDFAVVKREYVDPPVTKMRFNGK 60 VLVT DNLQVRVSGA + K+L+++ LRANGT+F+LGDFA V+REY DPP KMRFNGK Sbjct: 223 VLVTQTDNLQVRVSGALMTVKDLQDLDLRANGTSFKLGDFATVRREYRDPPQDKMRFNGK 282 Query: 61 EAIGLGVSMEKGGDIIALGRNLDRMSDELRTKLPVGIELERVSDQPKIVADSVGEFIHTL 120 E IGLG+SMEKGG+II LG++LD ++ KLPVG+EL RV+DQPK V SV EF+HTL Sbjct: 283 EVIGLGISMEKGGNIIELGKSLDSTLATIKAKLPVGVELSRVADQPKAVIASVSEFLHTL 342 Query: 121 VEAVLIVLAVSFIALGLHTRPLRIDVRPGLVVALTIPLVLAVTFLFMRIFDIDLHKISLG 180 VEAVLIVLAVSF+ALGLHT+P R+D+RPGLVV LTIPLVLA+TFLFMRI DIDLHKISLG Sbjct: 343 VEAVLIVLAVSFLALGLHTKPFRLDIRPGLVVGLTIPLVLAITFLFMRILDIDLHKISLG 402 Query: 181 ALIIALGLLVDDAIIAVEMMVRKMEEGLSRFDAATFAYTSTAIPMLTGTLITAAGFLPIG 240 ALIIALGLLVDDAIIAVEMMVRKMEEGLSRFDAATFAYTSTA+PMLTGTLITAAGFLPIG Sbjct: 403 ALIIALGLLVDDAIIAVEMMVRKMEEGLSRFDAATFAYTSTAMPMLTGTLITAAGFLPIG 462 Query: 241 LAKSAAGEYTFSMFSVNALALLISWLVAVVFTPYIGVLLLKVKPAANADGHHELFDTPFY 300 LAKSAAGEYTFSMFSVNALAL+ISWLVAV+FTPY+G LLLKVKPAA AD HHELF+TP Y Sbjct: 463 LAKSAAGEYTFSMFSVNALALIISWLVAVIFTPYLGYLLLKVKPAAGADAHHELFNTPMY 522 Query: 301 TRFRKLVTWCVEWRKTTILLTLTAFMLGLLGFRFIEQQFFPDSSRPELMIELWLPEGSAF 360 RFRK V WCVEWRKTTILLTL AF LG+ GF+FIEQQFFPDSSR ELM+ELWLPEGSAF Sbjct: 523 NRFRKTVNWCVEWRKTTILLTLAAFGLGVFGFKFIEQQFFPDSSRTELMVELWLPEGSAF 582 Query: 361 KATEAQAKKFEAFIRKQDGVESVTSYVGTGSPRFYLPLDQIFPQTNVSQIVVLPKDLKTR 420 KATEAQAKKFEAFI+KQDGVESVTSYVGTGSPRFYLPLDQIFPQTNVSQIVVL KDLK R Sbjct: 583 KATEAQAKKFEAFIQKQDGVESVTSYVGTGSPRFYLPLDQIFPQTNVSQIVVLAKDLKAR 642 Query: 421 ESLRLKIVDAFKNDFPGVRGRXDAFKNDFP 450 E+LRLKIV FK DFP VRGR N P Sbjct: 643 EALRLKIVAIFKTDFPEVRGRVKLLPNGPP 672 >D8NBL9.1 D8NBL9_RALSO Putative multidrug efflux transporter AcrB transmembrane domain Length = 1039 Score = 632 bits (1631), Expect = e-179, Method: Compositional matrix adjust. Identities = 305/453 (67%), Positives = 367/453 (81%), Gaps = 3/453 (0%) Query: 1 VLVTPGDNLQVRVSGAFHSPKELEEMQLRANGTT--FRLGDFAVVKREYVDPPVTKMRFN 58 VLVTP DN+Q+R+SG F ++LE + LR G T RLGD A V+ Y+DPP KMRFN Sbjct: 224 VLVTPTDNVQIRLSGQFSDIRDLENLTLRGPGGTSNIRLGDIATVRHGYIDPPRAKMRFN 283 Query: 59 GKEAIGLGVSMEKGGDIIALGRNLDRMSDELRTKLPVGIELERVSDQPKIVADSVGEFIH 118 GKE IGLG+SM KGGDII LG++L + +R KLPVGIE+++V DQP+ V SVGEF+H Sbjct: 284 GKEVIGLGISMAKGGDIIQLGKDLRATVERIRAKLPVGIEMQQVQDQPQSVQHSVGEFVH 343 Query: 119 TLVEAVLIVLAVSFIALGLHTRP-LRIDVRPGLVVALTIPLVLAVTFLFMRIFDIDLHKI 177 L+EAV+IVLAVSF++LGLHT+P LRIDV PGLVV LTIPLVLAVTFLFM IFDI LHKI Sbjct: 344 VLIEAVVIVLAVSFLSLGLHTKPRLRIDVWPGLVVGLTIPLVLAVTFLFMNIFDIGLHKI 403 Query: 178 SLGALIIALGLLVDDAIIAVEMMVRKMEEGLSRFDAATFAYTSTAIPMLTGTLITAAGFL 237 SLGALIIALGLLVDDAIIAVEMMVRK+EEG S+ +AATFAYTSTA+PMLTGTLITA GFL Sbjct: 404 SLGALIIALGLLVDDAIIAVEMMVRKLEEGFSKMEAATFAYTSTAMPMLTGTLITATGFL 463 Query: 238 PIGLAKSAAGEYTFSMFSVNALALLISWLVAVVFTPYIGVLLLKVKPAANADGHHELFDT 297 P+GLA+S GEYTF +F+V ALAL++SW+ AVVF PY+G LLL K A GHHELFDT Sbjct: 464 PVGLARSTVGEYTFGIFAVTALALVLSWVAAVVFVPYLGYLLLHTKSHAGDGGHHELFDT 523 Query: 298 PFYTRFRKLVTWCVEWRKTTILLTLTAFMLGLLGFRFIEQQFFPDSSRPELMIELWLPEG 357 PFY RFR V WCVE+RKT I++TL AF+LG+ GF+++E+QFFPDSSRPELM+ELWLPEG Sbjct: 524 PFYNRFRGWVNWCVEYRKTVIVITLAAFVLGVFGFKYVEKQFFPDSSRPELMVELWLPEG 583 Query: 358 SAFKATEAQAKKFEAFIRKQDGVESVTSYVGTGSPRFYLPLDQIFPQTNVSQIVVLPKDL 417 ++F TEA+AK+FEA IR+Q VESVT +VG+G+PRFYLPLDQI PQTNV+Q +V+P L Sbjct: 584 ASFSQTEAEAKRFEALIRQQKSVESVTFFVGSGAPRFYLPLDQILPQTNVAQAIVMPTSL 643 Query: 418 KTRESLRLKIVDAFKNDFPGVRGRXDAFKNDFP 450 +TRE +R +I+ K+ FP +RGR N P Sbjct: 644 ETREDVRQEIIGLLKSQFPQLRGRVKLLPNGPP 676 >C6BIS4.1 C6BIS4_RALP1 Acriflavin resistance protein Length = 1039 Score = 628 bits (1619), Expect = e-178, Method: Compositional matrix adjust. Identities = 301/453 (66%), Positives = 363/453 (80%), Gaps = 3/453 (0%) Query: 1 VLVTPGDNLQVRVSGAFHSPKELEEMQLRANG--TTFRLGDFAVVKREYVDPPVTKMRFN 58 VLVTP DN+Q+R+SG F ++LE + LR G T RLGD A VK YVDPP KMRFN Sbjct: 224 VLVTPTDNVQIRLSGQFSDIRDLENLTLRGPGGTTNIRLGDIATVKHGYVDPPHAKMRFN 283 Query: 59 GKEAIGLGVSMEKGGDIIALGRNLDRMSDELRTKLPVGIELERVSDQPKIVADSVGEFIH 118 GKE IGLG+SM KGGDII LG++L D++R KLP+GIE+++V +QPK V SVGEF+H Sbjct: 284 GKEVIGLGISMTKGGDIIQLGKDLRATVDKIRAKLPMGIEMQQVQNQPKSVQSSVGEFVH 343 Query: 119 TLVEAVLIVLAVSFIALGLHTRP-LRIDVRPGLVVALTIPLVLAVTFLFMRIFDIDLHKI 177 L+EAV+IVL VSF++LGLHT+P LRIDV PGLVV LTIPLVLAVTFLFM IFDI LHKI Sbjct: 344 VLIEAVVIVLGVSFLSLGLHTKPKLRIDVWPGLVVGLTIPLVLAVTFLFMNIFDIGLHKI 403 Query: 178 SLGALIIALGLLVDDAIIAVEMMVRKMEEGLSRFDAATFAYTSTAIPMLTGTLITAAGFL 237 SLGALIIALGLLVDDAIIAVEMMVRK+EEG S+ +AATFAYTSTA+PMLTGTLITA GFL Sbjct: 404 SLGALIIALGLLVDDAIIAVEMMVRKLEEGFSKMEAATFAYTSTAMPMLTGTLITATGFL 463 Query: 238 PIGLAKSAAGEYTFSMFSVNALALLISWLVAVVFTPYIGVLLLKVKPAANADGHHELFDT 297 P+GLA+S GEYTF +F+V ALAL++SW AVVF PY+G LLL+ K GHHELFDT Sbjct: 464 PVGLARSTVGEYTFGIFAVTALALVLSWFAAVVFVPYLGFLLLRTKSHVEGGGHHELFDT 523 Query: 298 PFYTRFRKLVTWCVEWRKTTILLTLTAFMLGLLGFRFIEQQFFPDSSRPELMIELWLPEG 357 PFY RFR V WCVE+RKT I++TL AF LG+ GF+++E+QFFPDSSRPELM+ELWLPEG Sbjct: 524 PFYNRFRGWVNWCVEYRKTVIVITLVAFGLGVFGFKYVEKQFFPDSSRPELMVELWLPEG 583 Query: 358 SAFKATEAQAKKFEAFIRKQDGVESVTSYVGTGSPRFYLPLDQIFPQTNVSQIVVLPKDL 417 S+F TEA+AK+FEA +RK+ V+SVT ++G+G+PRFYLPLDQI PQTNV+Q +V+P L Sbjct: 584 SSFNQTEAEAKRFEALMRKEQNVDSVTLFIGSGAPRFYLPLDQILPQTNVAQAIVMPTSL 643 Query: 418 KTRESLRLKIVDAFKNDFPGVRGRXDAFKNDFP 450 + RE +R ++ K+ FP +RGR N P Sbjct: 644 EAREGVRQHVIGLLKSQFPHLRGRVKLLPNGPP 676 >E2T3M2.1 E2T3M2_9RALS RND efflux transporter, hydrophobe/amphiphile efflux-1 (HAE1) family Length = 1039 Score = 628 bits (1619), Expect = e-178, Method: Compositional matrix adjust. Identities = 301/453 (66%), Positives = 363/453 (80%), Gaps = 3/453 (0%) Query: 1 VLVTPGDNLQVRVSGAFHSPKELEEMQLRANG--TTFRLGDFAVVKREYVDPPVTKMRFN 58 VLVTP DN+Q+R+SG F ++LE + LR G T RLGD A VK YVDPP KMRFN Sbjct: 224 VLVTPTDNVQIRLSGQFSDIRDLENLTLRGPGGTTNIRLGDIATVKHGYVDPPHAKMRFN 283 Query: 59 GKEAIGLGVSMEKGGDIIALGRNLDRMSDELRTKLPVGIELERVSDQPKIVADSVGEFIH 118 GKE IGLG+SM KGGDII LG++L D++R KLP+GIE+++V +QPK V SVGEF+H Sbjct: 284 GKEVIGLGISMTKGGDIIQLGKDLRATVDKIRAKLPMGIEMQQVQNQPKSVQSSVGEFVH 343 Query: 119 TLVEAVLIVLAVSFIALGLHTRP-LRIDVRPGLVVALTIPLVLAVTFLFMRIFDIDLHKI 177 L+EAV+IVL VSF++LGLHT+P LRIDV PGLVV LTIPLVLAVTFLFM IFDI LHKI Sbjct: 344 VLIEAVVIVLGVSFLSLGLHTKPKLRIDVWPGLVVGLTIPLVLAVTFLFMNIFDIGLHKI 403 Query: 178 SLGALIIALGLLVDDAIIAVEMMVRKMEEGLSRFDAATFAYTSTAIPMLTGTLITAAGFL 237 SLGALIIALGLLVDDAIIAVEMMVRK+EEG S+ +AATFAYTSTA+PMLTGTLITA GFL Sbjct: 404 SLGALIIALGLLVDDAIIAVEMMVRKLEEGFSKMEAATFAYTSTAMPMLTGTLITATGFL 463 Query: 238 PIGLAKSAAGEYTFSMFSVNALALLISWLVAVVFTPYIGVLLLKVKPAANADGHHELFDT 297 P+GLA+S GEYTF +F+V ALAL++SW AVVF PY+G LLL+ K GHHELFDT Sbjct: 464 PVGLARSTVGEYTFGIFAVTALALVLSWFAAVVFVPYLGFLLLRTKSHVEGGGHHELFDT 523 Query: 298 PFYTRFRKLVTWCVEWRKTTILLTLTAFMLGLLGFRFIEQQFFPDSSRPELMIELWLPEG 357 PFY RFR V WCVE+RKT I++TL AF LG+ GF+++E+QFFPDSSRPELM+ELWLPEG Sbjct: 524 PFYNRFRGWVNWCVEYRKTVIVITLVAFGLGVFGFKYVEKQFFPDSSRPELMVELWLPEG 583 Query: 358 SAFKATEAQAKKFEAFIRKQDGVESVTSYVGTGSPRFYLPLDQIFPQTNVSQIVVLPKDL 417 S+F TEA+AK+FEA +RK+ V+SVT ++G+G+PRFYLPLDQI PQTNV+Q +V+P L Sbjct: 584 SSFNQTEAEAKRFEALMRKEQNVDSVTLFIGSGAPRFYLPLDQILPQTNVAQAIVMPTSL 643 Query: 418 KTRESLRLKIVDAFKNDFPGVRGRXDAFKNDFP 450 + RE +R ++ K+ FP +RGR N P Sbjct: 644 EAREGVRQHVIGLLKSQFPHLRGRVKLLPNGPP 676 >Q8XYV2.1 Q8XYV2_RALSO Probable transmembrane drug efflux protein Length = 1039 Score = 627 bits (1618), Expect = e-177, Method: Compositional matrix adjust. Identities = 301/453 (66%), Positives = 365/453 (80%), Gaps = 3/453 (0%) Query: 1 VLVTPGDNLQVRVSGAFHSPKELEEMQLRANGTT--FRLGDFAVVKREYVDPPVTKMRFN 58 VLVTP DN+Q+R+SG F ++LE + LR G T RLGD A ++ Y+DPP KMRFN Sbjct: 224 VLVTPTDNVQIRLSGQFSDIRDLENLTLRGPGGTSNIRLGDIATIRHGYIDPPRAKMRFN 283 Query: 59 GKEAIGLGVSMEKGGDIIALGRNLDRMSDELRTKLPVGIELERVSDQPKIVADSVGEFIH 118 GKE IGLG+SM KGGDII LG++L + +R KLPVGIE+++V DQP+ V SVGEF+H Sbjct: 284 GKEVIGLGISMAKGGDIIQLGKDLRATVERIRAKLPVGIEMQQVQDQPQSVQHSVGEFVH 343 Query: 119 TLVEAVLIVLAVSFIALGLHTRP-LRIDVRPGLVVALTIPLVLAVTFLFMRIFDIDLHKI 177 L+EAV+IVLAVSF++LGLHT+P LRIDV PGLVV LTIPLVLAVTFLFM IFDI LHKI Sbjct: 344 VLIEAVVIVLAVSFLSLGLHTKPRLRIDVWPGLVVGLTIPLVLAVTFLFMNIFDIGLHKI 403 Query: 178 SLGALIIALGLLVDDAIIAVEMMVRKMEEGLSRFDAATFAYTSTAIPMLTGTLITAAGFL 237 SLGALIIALGLLVDDAIIAVEMMVRK+EEG S+ +AATFAYTSTA+PMLTGTLITA GFL Sbjct: 404 SLGALIIALGLLVDDAIIAVEMMVRKLEEGFSKMEAATFAYTSTAMPMLTGTLITATGFL 463 Query: 238 PIGLAKSAAGEYTFSMFSVNALALLISWLVAVVFTPYIGVLLLKVKPAANADGHHELFDT 297 P+GLA+S GEYTF +F+V ALAL++SW+ AVVF PY+G LLL K GHHELFDT Sbjct: 464 PVGLARSTVGEYTFGIFAVTALALVLSWVAAVVFVPYLGYLLLHTKSHVGDGGHHELFDT 523 Query: 298 PFYTRFRKLVTWCVEWRKTTILLTLTAFMLGLLGFRFIEQQFFPDSSRPELMIELWLPEG 357 PFY RFR V WCVE+RKT I++TL AF+LG+ GF+++E+QFFPDSSRPELM+ELWLPEG Sbjct: 524 PFYNRFRGWVNWCVEYRKTVIVITLAAFVLGVFGFKYVEKQFFPDSSRPELMVELWLPEG 583 Query: 358 SAFKATEAQAKKFEAFIRKQDGVESVTSYVGTGSPRFYLPLDQIFPQTNVSQIVVLPKDL 417 ++F TEA+AK+FEA IR+Q VESV ++G+G+PRFYLPLDQI PQTNV+Q +V+P L Sbjct: 584 ASFGQTEAEAKRFEALIRQQKSVESVAFFIGSGAPRFYLPLDQILPQTNVAQAIVMPTSL 643 Query: 418 KTRESLRLKIVDAFKNDFPGVRGRXDAFKNDFP 450 +TRE +R +I+ K+ FP +RGR N P Sbjct: 644 ETREDVRQEIIGLLKSQFPQLRGRVKLLPNGPP 676