BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0026.p (343 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IW66.1 D8IW66_HERSS D-alanine--D-alanine ligase EC=6.3.2.4 536 e-150 A4G8T6.1 DDL_HERAR D-alanine--D-alanine ligase EC=6.3.2.4 500 e-139 A6T2F6.1 DDL_JANMA D-alanine--D-alanine ligase EC=6.3.2.4 483 e-134 F1VUC0.1 F1VUC0_9BURK D-alanine-D-alanine ligase 479 e-133 C5CNF6.1 C5CNF6_VARPS D-alanine--D-alanine ligase EC=6.3.2.4 420 e-115 >D8IW66.1 D8IW66_HERSS D-alanine--D-alanine ligase EC=6.3.2.4 Length = 334 Score = 536 bits (1381), Expect = e-150, Method: Compositional matrix adjust. Identities = 256/333 (76%), Positives = 289/333 (86%), Gaps = 9/333 (2%) Query: 8 DQAAGFGKVGVLFGGRSAEREVSLMSGAGVLKALRSKGVDAHPFDPAERSLAELAAEKFD 67 DQ FG+VGVLFGGRSAEREVS+MSG GVL+ALRSKGVDAHPFDPAERSL EL AE+FD Sbjct: 5 DQIKAFGRVGVLFGGRSAEREVSMMSGNGVLQALRSKGVDAHPFDPAERSLGELQAEQFD 64 Query: 68 RVFIALHGRYGEDGTLQGALEQLGIPYTGPGVMASSIAMDKVMTKRIWIGHDLPTPRFIV 127 RVFIALHGRYGEDGTLQGALEQ+G+PYTGPGVM+SSIAMDKVMTKRIW+ H LPTPRF+ Sbjct: 65 RVFIALHGRYGEDGTLQGALEQMGLPYTGPGVMSSSIAMDKVMTKRIWLSHGLPTPRFLT 124 Query: 128 LDSASTPREELRQVPDVLGLPLMLKAPHEGSTIGIAKVAGYSDMQDGFNLCAQYDAVVLA 187 LD+ +T RE+LR VPD LGLPLMLKAPHEGSTIGIAKV GYSDMQ+GF+LCA+YD VVLA Sbjct: 125 LDAQATTREQLRLVPDTLGLPLMLKAPHEGSTIGIAKVNGYSDMQEGFDLCARYDKVVLA 184 Query: 188 EEFVAGRELTVPVMGKGTSGDNKAKAFPVIEIRAPEGNYDYQHKYFTDDTKYLCPAPIDE 247 EEF+ GRELTVPV+G G++ A+A PVIEIRAPEGNYDYQ+KYFTDDTKYLCPAP+DE Sbjct: 185 EEFIRGRELTVPVLGTGSA----ARALPVIEIRAPEGNYDYQNKYFTDDTKYLCPAPLDE 240 Query: 248 ALTQRVQX---KAYNALGCRGWSRVDFMLRASDN--EPFLLEINTSPGMTGHSLVPMSAR 302 LT+ +Q +A+NALGCRGWSRVDFMLR D EP+LLEINTSPGMTGHSLVPM+A+ Sbjct: 241 ELTRHLQNLAVQAFNALGCRGWSRVDFMLREVDGKAEPYLLEINTSPGMTGHSLVPMAAK 300 Query: 303 QAGMTYEDLCYEILKFAALDLKPSDAWKSHASK 335 GM+YEDLC EIL+ AALD+ PS W A + Sbjct: 301 AVGMSYEDLCCEILQMAALDMTPSKDWTPQAGQ 333 >A4G8T6.1 DDL_HERAR D-alanine--D-alanine ligase EC=6.3.2.4 Length = 334 Score = 500 bits (1288), Expect = e-139, Method: Compositional matrix adjust. Identities = 243/330 (73%), Positives = 275/330 (83%), Gaps = 8/330 (2%) Query: 1 MTQTMQIDQAAGFGKVGVLFGGRSAEREVSLMSGAGVLKALRSKGVDAHPFDPAERSLAE 60 MT T+Q + A FGKVGVLFGGRSAEREVSLMSG GVL AL+SKGVDAH FDPAERSLAE Sbjct: 1 MTTTVQ-NLKAEFGKVGVLFGGRSAEREVSLMSGKGVLAALQSKGVDAHAFDPAERSLAE 59 Query: 61 LAAEKFDRVFIALHGRYGEDGTLQGALEQLGIPYTGPGVMASSIAMDKVMTKRIWIGHDL 120 LAAEKFDRVFIALHG YGEDGTLQGALEQLGIPYTGPGVMAS+I+MDKVMTKR+ + H + Sbjct: 60 LAAEKFDRVFIALHGSYGEDGTLQGALEQLGIPYTGPGVMASAISMDKVMTKRVCLSHGV 119 Query: 121 PTPRFIVLDSASTPREELRQVPDVLGLPLMLKAPHEGSTIGIAKVAGYSDMQDGFNLCAQ 180 PTPRF VLD+ +T +L+ V GLPLMLKAPHEGSTIGIAKV MQ GF+LCA+ Sbjct: 120 PTPRFTVLDAETTSAAQLQSVAAEFGLPLMLKAPHEGSTIGIAKVETAEGMQAGFDLCAK 179 Query: 181 YDAVVLAEEFVAGRELTVPVMGKGTSGDNKAKAFPVIEIRAPEGNYDYQHKYFTDDTKYL 240 Y+AVVL E+FV GRELTVPV+G G A+A P++EIRAP+GNYDY+HKYF++DT+YL Sbjct: 180 YEAVVLVEQFVKGRELTVPVIGSG----RNARALPIVEIRAPQGNYDYEHKYFSNDTEYL 235 Query: 241 CPAPIDEALTQRVQX---KAYNALGCRGWSRVDFMLRASDNEPFLLEINTSPGMTGHSLV 297 CPAP DEA T+RVQ A+NA+GC GWSRVDFM+RASDNEPFLLEINTSPGMT HSLV Sbjct: 236 CPAPFDEAFTKRVQALAVSAFNAVGCTGWSRVDFMVRASDNEPFLLEINTSPGMTSHSLV 295 Query: 298 PMSARQAGMTYEDLCYEILKFAALDLKPSD 327 PMSA+ AG YEDLC EIL+ A +LKPS Sbjct: 296 PMSAKVAGTGYEDLCIEILRSAKTELKPSS 325 >A6T2F6.1 DDL_JANMA D-alanine--D-alanine ligase EC=6.3.2.4 Length = 320 Score = 483 bits (1242), Expect = e-134, Method: Compositional matrix adjust. Identities = 230/316 (72%), Positives = 264/316 (83%), Gaps = 7/316 (2%) Query: 13 FGKVGVLFGGRSAEREVSLMSGAGVLKALRSKGVDAHPFDPAERSLAELAAEKFDRVFIA 72 FGKVGVLFGGRSAEREVSLMSG GVL AL SKG+DAHPFDPAERSLAELAAEKFDRVFIA Sbjct: 5 FGKVGVLFGGRSAEREVSLMSGKGVLAALLSKGIDAHPFDPAERSLAELAAEKFDRVFIA 64 Query: 73 LHGRYGEDGTLQGALEQLGIPYTGPGVMASSIAMDKVMTKRIWIGHDLPTPRFIVLDSAS 132 LHGR+GEDG+LQGALEQLGIPYTGPGVMAS+IAMDKV+TKR+ + H +PTP+F L+ + Sbjct: 65 LHGRFGEDGSLQGALEQLGIPYTGPGVMASAIAMDKVITKRVCLSHGVPTPKFTALEVEA 124 Query: 133 TPREELRQVPDVLGLPLMLKAPHEGSTIGIAKVAGYSDMQDGFNLCAQYDAVVLAEEFVA 192 T E+L+ + G+PLMLKAPHEGSTIGIAKV MQ GF+LCA+YD VVL E+FV Sbjct: 125 TTAEQLQAIAAEFGMPLMLKAPHEGSTIGIAKVETAEGMQAGFDLCAKYDDVVLVEQFVK 184 Query: 193 GRELTVPVMGKGTSGDNKAKAFPVIEIRAPEGNYDYQHKYFTDDTKYLCPAPIDEALTQR 252 GRELTVPV+G G A+A P++EI AP+GNYDY+HKYF+DDT+YLCPAP D A T+R Sbjct: 185 GRELTVPVLGSG----RNARALPIVEIIAPQGNYDYEHKYFSDDTQYLCPAPFDAAFTKR 240 Query: 253 VQX---KAYNALGCRGWSRVDFMLRASDNEPFLLEINTSPGMTGHSLVPMSARQAGMTYE 309 VQ A+NA+GC GW+RVDF++R SDNEPFLLEINTSPGMTGHSLVPMSA+ AG YE Sbjct: 241 VQALAVSAFNAVGCTGWARVDFLVRESDNEPFLLEINTSPGMTGHSLVPMSAKVAGTAYE 300 Query: 310 DLCYEILKFAALDLKP 325 DLC EIL+ A L LKP Sbjct: 301 DLCVEILRMAKLGLKP 316 >F1VUC0.1 F1VUC0_9BURK D-alanine-D-alanine ligase Length = 322 Score = 479 bits (1233), Expect = e-133, Method: Compositional matrix adjust. Identities = 229/312 (73%), Positives = 265/312 (84%), Gaps = 8/312 (2%) Query: 13 FGKVGVLFGGRSAEREVSLMSGAGVLKALRSKGVDAHPFDPAERSLAELAAEKFDRVFIA 72 FGKVGVL GGRSAEREVSLMSG GVL ALRSKG+DAHPFDPAERSLAELA E+FDRVFIA Sbjct: 8 FGKVGVLLGGRSAEREVSLMSGNGVLAALRSKGIDAHPFDPAERSLAELATEQFDRVFIA 67 Query: 73 LHGRYGEDGTLQGALEQLGIPYTGPGVMASSIAMDKVMTKRIWIGHDLPTPRFIVLDSAS 132 LHGR+GEDGTLQGALE LGIPYTG GVMAS+I +DKVMTKRIW+ HDLPTPRF+VLD A Sbjct: 68 LHGRFGEDGTLQGALELLGIPYTGSGVMASAIGIDKVMTKRIWLSHDLPTPRFLVLD-AQ 126 Query: 133 TPREELRQVPDVLGLPLMLKAPHEGSTIGIAKVAGYSDMQDGFNLCAQYDAVVLAEEFVA 192 TP + L +VP+ LGLPLMLKAPHEGSTIGIAKV +DM GF LCAQYD+VVLAEEF++ Sbjct: 127 TPADVLDEVPEELGLPLMLKAPHEGSTIGIAKVIVDADMHTGFALCAQYDSVVLAEEFIS 186 Query: 193 GRELTVPVMGKGTSGDNKAKAFPVIEIRAPEGNYDYQHKYFTDDTKYLCPAPIDEALT-- 250 GRE+T+PV+G G + A+A P++EIRAPEGNYDY+HKYF+DDT YLCPAP+ + +T Sbjct: 187 GREITIPVLGSG----HGARALPIVEIRAPEGNYDYEHKYFSDDTVYLCPAPLADEVTAT 242 Query: 251 -QRVQXKAYNALGCRGWSRVDFMLRASDNEPFLLEINTSPGMTGHSLVPMSARQAGMTYE 309 Q + +A+NA+GC GW RVD MLRA D+ PFLLE+NTSPGMT HSLVPM+A+ AG++YE Sbjct: 243 LQDIAVRAFNAVGCTGWGRVDIMLRAGDDAPFLLEVNTSPGMTSHSLVPMAAKAAGISYE 302 Query: 310 DLCYEILKFAAL 321 DLC EIL A L Sbjct: 303 DLCVEILSTARL 314 >C5CNF6.1 C5CNF6_VARPS D-alanine--D-alanine ligase EC=6.3.2.4 Length = 323 Score = 420 bits (1080), Expect = e-115, Method: Compositional matrix adjust. Identities = 206/321 (64%), Positives = 241/321 (75%), Gaps = 7/321 (2%) Query: 5 MQIDQAAGFGKVGVLFGGRSAEREVSLMSGAGVLKALRSKGVDAHPFDPAERSLAELAAE 64 M + FGKV VLFGG SAERE+SL+SG GVL+ALRS+GVDAH FDPAER L EL E Sbjct: 1 MSLQDPKQFGKVAVLFGGSSAEREISLLSGNGVLEALRSRGVDAHAFDPAERDLVELRRE 60 Query: 65 KFDRVFIALHGRYGEDGTLQGALEQLGIPYTGPGVMASSIAMDKVMTKRIWIGHDLPTPR 124 F R FIALHGR+GEDGT+QGALE LGIPYTG GVMASS+AMDKVMTKRIW LPTPR Sbjct: 61 GFARCFIALHGRHGEDGTVQGALELLGIPYTGSGVMASSVAMDKVMTKRIWQADGLPTPR 120 Query: 125 FIVLDSASTPREELRQVPDVLGLPLMLKAPHEGSTIGIAKVAGYSDMQDGFNLCAQYDAV 184 ++ L RE++R VPDVLGLPL++K P EGS+IG+ KV GYS MQD L A+YDA Sbjct: 121 YVRLAFDQQSREQVRAVPDVLGLPLIVKPPREGSSIGVTKVEGYSQMQDAVTLSARYDAD 180 Query: 185 VLAEEFVAGRELTVPVMGKGTSGDNKAKAFPVIEIRAPEGNYDYQHKYFTDDTKYLCPAP 244 VL EEF+ G E+T V+G+G A+A PV+ I APEG YDYQ+KYFTDD KY CP+ Sbjct: 181 VLCEEFIEGEEVTCAVLGQGLD----ARALPVVRIAAPEGAYDYQNKYFTDDVKYHCPSG 236 Query: 245 IDEA---LTQRVQXKAYNALGCRGWSRVDFMLRASDNEPFLLEINTSPGMTGHSLVPMSA 301 + EA QR+ AY+ LGCRGW R D M+RASD +PFLLE+NTSPGMT HSLVPMSA Sbjct: 237 LPEAEEHEIQRITLAAYHTLGCRGWGRADVMIRASDRKPFLLEMNTSPGMTSHSLVPMSA 296 Query: 302 RQAGMTYEDLCYEILKFAALD 322 R +G+ YEDLC +L A+LD Sbjct: 297 RASGIAYEDLCLRVLASASLD 317