BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0412.p (371 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5NED2.1 C5NED2_BURMA tRNA 5-methylaminomethyl-2-thiouridine bio... 96 8e-18 A1V8X2.2 MNMC_BURMS tRNA 5-methylaminomethyl-2-thiouridine biosy... 96 1e-17 A4LMS3.1 A4LMS3_BURPS tRNA 5-methylaminomethyl-2-thiouridine bio... 94 3e-17 C0XWF4.1 C0XWF4_BURPS tRNA 5-methylaminomethyl-2-thiouridine bio... 94 3e-17 B7CVL8.1 B7CVL8_BURPS tRNA 5-methylaminomethyl-2-thiouridine bio... 94 4e-17 >C5NED2.1 C5NED2_BURMA tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC Length = 708 Score = 95.9 bits (237), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 75/220 (34%), Positives = 99/220 (45%), Gaps = 33/220 (15%) Query: 20 WRGRAVYTILQAGYSDA--FLQAWAALREQGDDVDTPQRLHYLLIDAALPTAQQLR---- 73 WR +TI++ G+ FL WAA R D +RLH++ ++ + LR Sbjct: 102 WRHERAFTIIETGFGTGCNFLATWAAWRA---DPSHCERLHFVSVEKHPFAREDLRRAAA 158 Query: 74 ------AMAPTNALAGELANVSPPLTPGYHRLLLEQGRVVLTLIIGEVAASLAQVDARVD 127 + P L ELAN P LTPG HRL + GRV LTL+ G+ L + R Sbjct: 159 HIVAYTTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDVLPNLALRAH 218 Query: 128 LFLL------QDAAMWNAPQLKRLARLATPRAQLRMGGAWRASSAELAAAGFTMDEDD-- 179 F L ++A +W+ K LA+LA RA + A L AGF + D Sbjct: 219 AFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKVDGF 278 Query: 180 -----KLTAHFTPRWKV----AERAVPQDRR-ALVIGAGL 209 L F PRW+V RA DRR A+VIGAGL Sbjct: 279 AGKRAMLVGEFAPRWRVRRHEPPRAFSTDRRDAIVIGAGL 318 >A1V8X2.2 MNMC_BURMS tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC tRNA (mnm(5)s(2)U34)-methyltransferase FAD-dependent cmnm(5)s(2)U34 oxidoreductase EC=2.1.1.61 EC=1.5.-.- Length = 657 Score = 95.5 bits (236), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 75/220 (34%), Positives = 99/220 (45%), Gaps = 33/220 (15%) Query: 20 WRGRAVYTILQAGYSDA--FLQAWAALREQGDDVDTPQRLHYLLIDAALPTAQQLR---- 73 WR +TI++ G+ FL WAA R D +RLH++ ++ + LR Sbjct: 51 WRHERAFTIIETGFGTGCNFLATWAAWRA---DPSHCERLHFVSVEKHPFAREDLRRAAA 107 Query: 74 ------AMAPTNALAGELANVSPPLTPGYHRLLLEQGRVVLTLIIGEVAASLAQVDARVD 127 + P L ELAN P LTPG HRL + GRV LTL+ G+ L + R Sbjct: 108 HIVAYTTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDVLPNLALRAH 167 Query: 128 LFLL------QDAAMWNAPQLKRLARLATPRAQLRMGGAWRASSAELAAAGFTMDEDD-- 179 F L ++A +W+ K LA+LA RA + A L AGF + D Sbjct: 168 AFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKVDGF 227 Query: 180 -----KLTAHFTPRWKV----AERAVPQDRR-ALVIGAGL 209 L F PRW+V RA DRR A+VIGAGL Sbjct: 228 AGKRAMLVGEFAPRWRVRRHEPPRAFSTDRRDAIVIGAGL 267 >A4LMS3.1 A4LMS3_BURPS tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC Length = 734 Score = 94.4 bits (233), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 76/223 (34%), Positives = 100/223 (44%), Gaps = 36/223 (16%) Query: 20 WRGRAVYTILQAGYSDA--FLQAWAALREQGDDVDTPQRLHYLLIDAALPTAQQLRAMA- 76 WR +TI++ G+ FL WAA R D +RLH++ ++ + LR A Sbjct: 125 WRHERAFTIIETGFGTGCNFLATWAAWRA---DPSHCERLHFVSVEKHPFAREDLRRAAA 181 Query: 77 ------------PTNALAGELANVSPPLTPGYHRLLLEQGRVVLTLIIGEVAASLAQVDA 124 P L ELAN P LTPG HRL + GRV LTL+ G+ L + Sbjct: 182 HIVAYTTITTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDVLPNLAL 241 Query: 125 RVDLFLL------QDAAMWNAPQLKRLARLATPRAQLRMGGAWRASSAELAAAGFTMDED 178 R F L ++A +W+ K LA+LA RA + A L AGF + Sbjct: 242 RAHAFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKV 301 Query: 179 D-------KLTAHFTPRWKV----AERAVPQDRR-ALVIGAGL 209 D L F PRW+V RA+ DRR A+VIGAGL Sbjct: 302 DGFAGKRAMLVGEFAPRWRVRRHEPPRALSTDRRDAIVIGAGL 344 >C0XWF4.1 C0XWF4_BURPS tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC Length = 711 Score = 94.4 bits (233), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 76/223 (34%), Positives = 100/223 (44%), Gaps = 36/223 (16%) Query: 20 WRGRAVYTILQAGYSDA--FLQAWAALREQGDDVDTPQRLHYLLIDAALPTAQQLRAMA- 76 WR +TI++ G+ FL WAA R D +RLH++ ++ + LR A Sbjct: 102 WRHERAFTIIETGFGTGCNFLATWAAWRA---DPSHCERLHFVSVEKHPFAREDLRRAAA 158 Query: 77 ------------PTNALAGELANVSPPLTPGYHRLLLEQGRVVLTLIIGEVAASLAQVDA 124 P L ELAN P LTPG HRL + GRV LTL+ G+ L + Sbjct: 159 HIVAYTTITTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDVLPNLAL 218 Query: 125 RVDLFLL------QDAAMWNAPQLKRLARLATPRAQLRMGGAWRASSAELAAAGFTMDED 178 R F L ++A +W+ K LA+LA RA + A L AGF + Sbjct: 219 RAHAFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKV 278 Query: 179 D-------KLTAHFTPRWKV----AERAVPQDRR-ALVIGAGL 209 D L F PRW+V RA+ DRR A+VIGAGL Sbjct: 279 DGFAGKRAMLVGEFAPRWRVRRHEPPRALSTDRRDAIVIGAGL 321 >B7CVL8.1 B7CVL8_BURPS tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC Length = 711 Score = 94.0 bits (232), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 76/223 (34%), Positives = 99/223 (44%), Gaps = 36/223 (16%) Query: 20 WRGRAVYTILQAGYSDA--FLQAWAALREQGDDVDTPQRLHYLLIDAALPTAQQLRAMA- 76 WR +TI++ G+ FL WAA R D +RLH++ ++ + LR A Sbjct: 102 WRHERAFTIIETGFGTGCNFLATWAAWRA---DPSHCERLHFVSVEKHPFAREDLRRAAA 158 Query: 77 ------------PTNALAGELANVSPPLTPGYHRLLLEQGRVVLTLIIGEVAASLAQVDA 124 P L ELAN P LTPG HRL + GRV LTL+ G+ L + Sbjct: 159 HIVAYTTITTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDVLPNLAL 218 Query: 125 RVDLFLL------QDAAMWNAPQLKRLARLATPRAQLRMGGAWRASSAELAAAGFTMDED 178 R F L ++A +W+ K LA+LA RA + A L AGF + Sbjct: 219 RAHAFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKV 278 Query: 179 D-------KLTAHFTPRWKV----AERAVPQDRR-ALVIGAGL 209 D L F PRW+V RA DRR A+VIGAGL Sbjct: 279 DGFAGKRAMLVGEFAPRWRVRRHEPPRAFSTDRRDAIVIGAGL 321