BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0411.p (205 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IZ19.1 D8IZ19_HERSS tRNA 5-methylaminomethyl-2-thiouridine bio... 196 2e-48 F1W144.1 F1W144_9BURK FAD dependent oxidoreductase 184 5e-45 B5WD03.1 B5WD03_9BURK tRNA 5-methylaminomethyl-2-thiouridine bio... 162 2e-38 Q39L16.1 MNMC_BURS3 tRNA 5-methylaminomethyl-2-thiouridine biosy... 162 3e-38 C7RQL6.1 C7RQL6_ACCPU tRNA U-34 5-methylaminomethyl-2-thiouridin... 160 8e-38 >D8IZ19.1 D8IZ19_HERSS tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC Length = 625 Score = 196 bits (497), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 114/201 (56%), Positives = 131/201 (65%), Gaps = 23/201 (11%) Query: 6 AAAPVVILANGMQSAALAQAAGLPLQTIRGQVSHLPAASLPDLPFVLCGDGYLTGAVNGL 65 A APVVILA G Q++AL AA LPL +RGQV+HLPA P LPF LCGDGYLT A GL Sbjct: 432 AQAPVVILAAGAQASALPIAAALPLNAVRGQVTHLPAEGFPRLPFALCGDGYLTRAHGGL 491 Query: 66 VSVGASYDRDNHDTALRADSHAGNLDKLRQLLPGVS-----PDIDPDGLSGRVGFRCVSA 120 V +GA+YDR D ALR DSHA NL +L+ +LP ++ P L GRVG RC++ Sbjct: 492 VCMGATYDR-FQDPALRVDSHAENLGRLQHMLPQIATHTHLPPASAPSLQGRVGQRCIAP 550 Query: 121 DRLPLVGALPDQAALAAGGEVQLRDAPRWPQLYGLLAYASRGLIWAPLAAEIVACALEDE 180 DRLPLVGALP QA LYGLL YASRGLIWAPL AE++A LE E Sbjct: 551 DRLPLVGALPGQAG-----------------LYGLLGYASRGLIWAPLMAELLAAMLERE 593 Query: 181 PAPLGKDLLALLDPARFALKA 201 P PL +DL+A LDPARF A Sbjct: 594 PLPLPRDLVAALDPARFGTTA 614 >F1W144.1 F1W144_9BURK FAD dependent oxidoreductase Length = 541 Score = 184 bits (468), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 114/202 (56%), Positives = 139/202 (68%), Gaps = 7/202 (3%) Query: 6 AAAPVVILANGMQSAALAQAAGLPLQTIRGQVSHL--PAASLPDLPFVLCGDGYLTGAVN 63 A AP VI ANG + AQ AGLPLQ +RGQV+H+ PA+S P L V+C +GYLT V Sbjct: 343 AQAPTVIFANGNGATGFAQLAGLPLQAVRGQVTHVSAPASSAPAL--VVCREGYLTPPVQ 400 Query: 64 GLVSVGASYDRDNHDTALRADSHAGNLDKLRQLLPGVSPDIDPDGLSGRVGFRCVSADRL 123 G+ GA+YD+D+ ALR S NL +++ +LPG+ PD L+GRVGFRCV+ DRL Sbjct: 401 GVRCAGATYDQDD-ALALRWSSQHDNLQRVQAMLPGLWPDTSGFALAGRVGFRCVAPDRL 459 Query: 124 PLVGALPDQAALAAGGEVQLRDAPRWPQLYGLLAYASRGLIWAPLAAEIVACALEDEPAP 183 PLVGALP A+ +LRD PRWP L+GLL YASRGLIWAPLAAE++A LE EP P Sbjct: 460 PLVGALP--VTTASSRAERLRDVPRWPGLHGLLGYASRGLIWAPLAAELLAAQLEGEPLP 517 Query: 184 LGKDLLALLDPARFALKAHRKN 205 L DL LDPARF LKA R++ Sbjct: 518 LESDLADALDPARFRLKALRQS 539 >B5WD03.1 B5WD03_9BURK tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC Length = 655 Score = 162 bits (411), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 92/207 (44%), Positives = 133/207 (64%), Gaps = 9/207 (4%) Query: 6 AAAPVVILANGMQSAALAQAAGLPLQTIRGQVSHLPAASLPDLPFVLCGDGYLTGAVNGL 65 A APVVI+A+ ++A +A P ++IRGQ+S LPA ++ L + G+GY NG+ Sbjct: 446 ARAPVVIVASAHEAARIAGLRHAPTRSIRGQLSLLPAGTVAPLALPVIGEGYAVPLANGV 505 Query: 66 VSVGASYDRDNHDTALRADSHAGNLDKLRQLLPGVSPDID---PDGLSGRVGFRCVSADR 122 GA+Y+ D+ DT+LRAD H N++++ Q+LP + +D L+GRV FRCV++DR Sbjct: 506 TLTGATYELDDPDTSLRADGHRENIERVAQMLPAFASALDAAPQTSLAGRVAFRCVTSDR 565 Query: 123 LPLVGALPDQAALAAGGEVQLR-----DAPRWPQLYGLLAYASRGLIWAPLAAEIVACAL 177 +P++G L D+AA A+ +LR D PR LYG AY SRGL+WA L AE++A + Sbjct: 566 MPMIGQLADEAA-ASRDAARLRGAWPLDLPRVDGLYGAFAYGSRGLVWAALGAELIASQI 624 Query: 178 EDEPAPLGKDLLALLDPARFALKAHRK 204 E EP PL +DL +DPARF L+A R+ Sbjct: 625 EGEPWPLERDLAEDIDPARFLLRALRQ 651 >Q39L16.1 MNMC_BURS3 tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC tRNA (mnm(5)s(2)U34)-methyltransferase FAD-dependent cmnm(5)s(2)U34 oxidoreductase EC=2.1.1.61 EC=1.5.-.- Length = 648 Score = 162 bits (409), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 95/203 (46%), Positives = 125/203 (61%), Gaps = 5/203 (2%) Query: 6 AAAPVVILANGMQSAALAQAAGLPLQTIRGQVSHLPAASLPDLPFVLCGDGYLTGAVNGL 65 A APVV+LAN +A LA + LQ +RGQ++ LP S LP GDGY +G Sbjct: 438 AEAPVVVLANAGDAARLAGLQHVALQPVRGQLTLLPPGSTAPLPCPTIGDGYAVPLDDGT 497 Query: 66 VSVGASYDRDNHDTALRADSHAGNLDKLRQLLPGVSPDI-DPDGLSGRVGFRCVSADRLP 124 + +GA+++ D+ D A+RA H NL+++R LLPG+ D+ DPD L GRV FR V DRLP Sbjct: 498 LLIGATFEPDDVDPAMRAAGHLENLERVRHLLPGLIGDLPDPDTLRGRVAFRWVVGDRLP 557 Query: 125 LVGALPDQAALAAGGE----VQLRDAPRWPQLYGLLAYASRGLIWAPLAAEIVACALEDE 180 L+G L D+A A Q RD PR P LYG + SRGL+WA L AE++A LE E Sbjct: 558 LLGPLADEAGAIANARALSGAQARDLPRLPGLYGAFGFGSRGLVWAALGAELIASQLEGE 617 Query: 181 PAPLGKDLLALLDPARFALKAHR 203 P PL ++L +DPARF ++A R Sbjct: 618 PWPLERELADAVDPARFLIRALR 640 >C7RQL6.1 C7RQL6_ACCPU tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC Length = 404 Score = 160 bits (406), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 95/200 (47%), Positives = 128/200 (64%), Gaps = 5/200 (2%) Query: 3 AAAAAAPVVILANGMQSAALAQAAGLPLQTIRGQVSHLPAA--SLPDLPFVLCGDGYLTG 60 A A APV+ILAN + A AA LPL+ RGQVSHLPAA S PD+ V+C GY+T Sbjct: 191 AVIAEAPVLILANATDAGRFAVAAHLPLRAARGQVSHLPAAAGSAPDI--VVCRLGYVTP 248 Query: 61 AVNGLVSVGASYDRDNHDTALRADSHAGNLDKLRQLLPGVSPDIDPDGLSGRVGFRCVSA 120 A++GL GA++ D+ +TALR HA NL +L +LPG + I P L GRVGFR ++ Sbjct: 249 AIDGLRCAGATFSIDDGETALRTADHAENLARLAFILPGHAKGIQPADLGGRVGFRPLAP 308 Query: 121 DRLPLVGALPDQAALAAGGEVQ-LRDAPRWPQLYGLLAYASRGLIWAPLAAEIVACALED 179 DRLP+VGA+ D A+ A + L + PR P LY + + +RG++W+ LA E++AC + Sbjct: 309 DRLPMVGAVADATAIEASHPSRPLAEMPRHPGLYVINGFGARGIVWSALAGELLACLITG 368 Query: 180 EPAPLGKDLLALLDPARFAL 199 P PL DL++ LDP RF L Sbjct: 369 APLPLADDLVSALDPGRFLL 388