BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0239.p (463 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8J0V2.1 D8J0V2_HERSS Methylenomycin A resistance protein EC=3.6... 607 e-171 E5U4T6.1 E5U4T6_ALCXX Major facilitator superfamily transporter ... 442 e-122 E3HLD7.1 E3HLD7_ACHXA Methylenomycin A resistance protein 438 e-121 C2YN27.1 C2YN27_BACCE Methylenomycin A resistance protein 392 e-107 E8VE14.1 E8VE14_BACST Toxic compound efflux transporter 389 e-106 >D8J0V2.1 D8J0V2_HERSS Methylenomycin A resistance protein EC=3.6.3.- Length = 478 Score = 607 bits (1566), Expect = e-171, Method: Compositional matrix adjust. Identities = 335/441 (75%), Positives = 372/441 (84%) Query: 4 QVGLLPLVTLAIGFVMAMLDVTVVNVGLSSIEKSLATPLSMLVWIVDGYTLSFAAMLLVG 63 + G+LPLV LAIGFVMAMLDVTVVNVGLS+I SL PLS LVWIVDGYTL+FAAMLLVG Sbjct: 26 RAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVG 85 Query: 64 GALADRYGAKNIYLLGLVLFVVASLLCGAAPNGGMLVAARLLQGLGAAFFMPSSLSLLTH 123 GALADR+GA+ +YL GL LFV+ASLLCGAAPNG L+ ARLLQGLGAAFFMPSSLSLLTH Sbjct: 86 GALADRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTH 145 Query: 124 VYEDDRVRARMLGVWSATVGGAAAVGPLVGGILIHSFGWRSVFLINVPVGLLGLYMAVKM 183 VYEDDRVRARMLGVWSATVG AAAVGPLVGG+LIH GWRSVFLINVPVGL+GL MA K+ Sbjct: 146 VYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKL 205 Query: 184 IPSVERHVRALTIASHFLGVLMLAALSFVLIEGPVYGWTSLPIATAVVVTMLAFGTLVQR 243 IP V H RAL ++SH GV+MLA LSFVLI+GPVYGWTS I + V + A L++ Sbjct: 206 IPQVGGHARALNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRH 265 Query: 244 ERSGSAPLLPRALFATPQFAAANGIGFLINFGVYGQFFFLALFLQQARGADALQTGLQML 303 E G APLLP+ALFATPQFAAANGIGFLINFGVYG F+LALFLQQ RGADALQTGLQ++ Sbjct: 266 ELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLV 325 Query: 304 PMMAVIFIGNLNSGRMTARFGPRLPLRLGLTVGAVFSAVQIGLTPETPYWMLAVAGALAN 363 PMMAVIF GNL SGRM+A +GPR+PL LGL+VG VF+A+ +G +P TPYW+LA+ A AN Sbjct: 326 PMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTVFTALAMGFSPATPYWLLALVCASAN 385 Query: 364 LGISTAIPAMTTAVMQVAGKGHANSAAAALNANRQIGALVGVALMGAILHSFGNWITRIV 423 LGISTAIPAMTT VMQVAGK HANSAAAALNANRQIGALVGVALMGAILHS W R Sbjct: 386 LGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVTGWNLRNP 445 Query: 424 LAYGVVAIVYALALVLVLRYV 444 LA+GV+A YA AL LV R+V Sbjct: 446 LAFGVMAAGYASALWLVWRFV 466 >E5U4T6.1 E5U4T6_ALCXX Major facilitator superfamily transporter MFS_1 Length = 467 Score = 442 bits (1137), Expect = e-122, Method: Compositional matrix adjust. Identities = 274/451 (60%), Positives = 332/451 (73%), Gaps = 3/451 (0%) Query: 3 AQVGLLPLVTLAIGFVMAMLDVTVVNVGLSSIEKSLATPLSMLVWIVDGYTLSFAAMLLV 62 A GLLPLVTLAIGFVMAMLDVTVVNV L SI + PL+ LVWIVDGYTL+FAA+LLV Sbjct: 19 AATGLLPLVTLAIGFVMAMLDVTVVNVALPSIAQQFTVPLTDLVWIVDGYTLTFAALLLV 78 Query: 63 GGALADRYGAKNIYLLGLVLFVVASLLCGAAPNGGMLVAARLLQGLGAAFFMPSSLSLLT 122 GALADRYGAK +YL GL +F +ASLLCG AP G +L+AAR+LQGLGAA FMPSSLSLLT Sbjct: 79 AGALADRYGAKTVYLAGLGVFTLASLLCGIAPGGDLLIAARMLQGLGAALFMPSSLSLLT 138 Query: 123 HVYEDDRVRARMLGVWSATVGGAAAVGPLVGGILIHSFGWRSVFLINVPVGLLGLYMAVK 182 H YEDD+VR RML WSA V A A GPL+GG+LIH FGWR +FLIN+P+GL GL++A + Sbjct: 139 HAYEDDQVRNRMLAAWSAIVAVAGAAGPLLGGVLIHQFGWRGIFLINLPLGLAGLWLARR 198 Query: 183 MIPSVERHVRALTIASHFLGVLMLAALSFVLIEGPVYGWTSLPIATAVVVTMLAFGTLVQ 242 I + R RAL SH GV+ L++L F LI+G YGW SLPI A +V +LA L+ Sbjct: 199 RIQAAPRRPRALNPLSHVFGVVALSSLCFALIQGNAYGWDSLPILGAALVFLLAATLLIH 258 Query: 243 RERSGSAPLLPRALFATPQFAAANGIGFLINFGVYGQFFFLALFLQQARGADALQTGLQM 302 RER + P++PRALF T QFAAANG+GFLIN YGQ F L+LFLQQARGADAL+TG+Q+ Sbjct: 259 RERHHAEPIIPRALFQTTQFAAANGVGFLINLASYGQLFLLSLFLQQARGADALETGIQL 318 Query: 303 LPMMAVIFIGNLNSGRMTARFGPRLPLRLGLTVGAVFSAVQI-GLTPETPYWMLAVAGAL 361 +PM+AV IGNL S R++AR+ L G+ + V S + TP+ PY A AL Sbjct: 319 VPMLAVFSIGNLISSRISARWNVSASLLGGILLATVMSLAGLAAFTPDMPYRPFAAVVAL 378 Query: 362 ANLGISTAIPAMTTAVMQVAGKGHANSAAAALNANRQIGALVGVALMGAILHSFGNWITR 421 ANLG+ A+PAMT+ VMQV+GK HANSAAAALNANRQ GALVGVALMG ILH +W Sbjct: 379 ANLGVGIAVPAMTSVVMQVSGKHHANSAAAALNANRQSGALVGVALMGTILHLLPDWHAS 438 Query: 422 IVLAYGVVAIVYALALVLVLRYVNARSAAST 452 + +Y ++A Y +A +LV R++ R A +T Sbjct: 439 LPASYVIIAAAYLVAAMLVWRHL--RHARNT 467 >E3HLD7.1 E3HLD7_ACHXA Methylenomycin A resistance protein Length = 466 Score = 438 bits (1126), Expect = e-121, Method: Compositional matrix adjust. Identities = 272/449 (60%), Positives = 332/449 (73%), Gaps = 2/449 (0%) Query: 3 AQVGLLPLVTLAIGFVMAMLDVTVVNVGLSSIEKSLATPLSMLVWIVDGYTLSFAAMLLV 62 A GLLPLVTLAIGFVMAMLDVTVVNV L SI PL+ LVWIVDGYTL+FAA+LLV Sbjct: 18 AAAGLLPLVTLAIGFVMAMLDVTVVNVALPSIAVQFTVPLTDLVWIVDGYTLTFAALLLV 77 Query: 63 GGALADRYGAKNIYLLGLVLFVVASLLCGAAPNGGMLVAARLLQGLGAAFFMPSSLSLLT 122 GALADRYGAK +YL GL +F +ASLLCG APN +L+ AR+LQGLGAA FMPSSLSLLT Sbjct: 78 AGALADRYGAKTVYLAGLGVFTLASLLCGLAPNADLLILARMLQGLGAALFMPSSLSLLT 137 Query: 123 HVYEDDRVRARMLGVWSATVGGAAAVGPLVGGILIHSFGWRSVFLINVPVGLLGLYMAVK 182 H Y+D++ R RML WSA V A A GPL+GG+LIH FGWR +FLIN+P+GL GL++A + Sbjct: 138 HAYDDEQTRNRMLAAWSAIVAVAGAAGPLLGGVLIHQFGWRGIFLINIPLGLAGLWLARR 197 Query: 183 MIPSVERHVRALTIASHFLGVLMLAALSFVLIEGPVYGWTSLPIATAVVVTMLAFGTLVQ 242 I + R RAL SH LGV+ L++L FVLI+G YGW+S I + + A LV+ Sbjct: 198 RIQAAPRRPRALNPLSHLLGVIALSSLCFVLIQGNAYGWSSSLILGMAALCLAAAALLVR 257 Query: 243 RERSGSAPLLPRALFATPQFAAANGIGFLINFGVYGQFFFLALFLQQARGADALQTGLQM 302 RER + P++PRALFAT QFAAANG+GFLIN YGQ F L+LFLQQARG DALQTGLQ+ Sbjct: 258 RERRHAEPIIPRALFATAQFAAANGVGFLINLASYGQLFLLSLFLQQARGTDALQTGLQL 317 Query: 303 LPMMAVIFIGNLNSGRMTARFGPRLPLRLGLTVGAVFSAV-QIGLTPETPYWMLAVAGAL 361 +PM+AV IGNL SGR++AR+ L GL++ A+ S++ +P+ YW AVA AL Sbjct: 318 VPMLAVFSIGNLMSGRISARWNVSTALLGGLSLAAIMSSMGTFAFSPDMAYWPFAVAVAL 377 Query: 362 ANLGISTAIPAMTTAVMQVAGKGHANSAAAALNANRQIGALVGVALMGAILHSFGNWITR 421 +NLG+ A+PAMT+ VMQV+GK HANSAAAALNANRQ GALVGVALMG ILH +W Sbjct: 378 SNLGVGIAVPAMTSVVMQVSGKSHANSAAAALNANRQSGALVGVALMGTILHLLPDWHAS 437 Query: 422 IVLAYGVVAIVYALALVLVLRYV-NARSA 449 + AY ++ Y A+ LV R++ AR+A Sbjct: 438 LPAAYCIIGASYLAAVALVWRHLRRARNA 466 >C2YN27.1 C2YN27_BACCE Methylenomycin A resistance protein Length = 459 Score = 392 bits (1008), Expect = e-107, Method: Compositional matrix adjust. Identities = 209/446 (46%), Positives = 298/446 (66%) Query: 2 DAQVGLLPLVTLAIGFVMAMLDVTVVNVGLSSIEKSLATPLSMLVWIVDGYTLSFAAMLL 61 + G++ L+TLA+GF+MA LDVTVVN+ + +I+++L L WIVDGY LSFAA+LL Sbjct: 14 KEKSGIVILITLAMGFIMATLDVTVVNIAVVNIQETLGLTLYSSTWIVDGYILSFAALLL 73 Query: 62 VGGALADRYGAKNIYLLGLVLFVVASLLCGAAPNGGMLVAARLLQGLGAAFFMPSSLSLL 121 GGALA++YGAKN+Y++GL++FV ASLLC A NG L+ R+LQG+GAA FMPSSLSLL Sbjct: 74 AGGALANQYGAKNMYMVGLIIFVFASLLCAMATNGNTLIIGRVLQGVGAALFMPSSLSLL 133 Query: 122 THVYEDDRVRARMLGVWSATVGGAAAVGPLVGGILIHSFGWRSVFLINVPVGLLGLYMAV 181 + D++ RA+M G+WSA V A+ GP +GG+L+++FGWRS+F+IN+P+G++G+ MA Sbjct: 134 VVSFPDEKKRAKMFGIWSAIVSIASGTGPFIGGLLVNTFGWRSIFIINLPIGVIGVLMAF 193 Query: 182 KMIPSVERHVRALTIASHFLGVLMLAALSFVLIEGPVYGWTSLPIATAVVVTMLAFGTLV 241 +IP+V + + +H +G+ + L+F LIEGP YGW+S I VV T+L V Sbjct: 194 VIIPNVLPKKEKINVLNHLIGITTITLLAFTLIEGPSYGWSSFQILGGVVGTVLTAILFV 253 Query: 242 QRERSGSAPLLPRALFATPQFAAANGIGFLINFGVYGQFFFLALFLQQARGADALQTGLQ 301 ER ++PR LF F++AN +GFL+NF V+G F LFLQ+A GA GLQ Sbjct: 254 YAERKSKNSVVPRVLFQNETFSSANIVGFLLNFAVFGGIFMFGLFLQKAYGASPFVAGLQ 313 Query: 302 MLPMMAVIFIGNLNSGRMTARFGPRLPLRLGLTVGAVFSAVQIGLTPETPYWMLAVAGAL 361 +LPMM+V IGNL +M +FG +L L + L + ++ S + + + Y +A ++ Sbjct: 314 LLPMMSVFVIGNLLFAKMVTKFGSKLLLFIALFIASIGSLLLMFIVNNVSYIGIAFIYSI 373 Query: 362 ANLGISTAIPAMTTAVMQVAGKGHANSAAAALNANRQIGALVGVALMGAILHSFGNWITR 421 NLGI A+PAMTT VM+ AG+ + N A A LN NRQIGALVG+A+MG IL+ NW Sbjct: 374 VNLGIGIAVPAMTTIVMKSAGRENGNMAGATLNVNRQIGALVGIAIMGIILNESSNWYRG 433 Query: 422 IVLAYGVVAIVYALALVLVLRYVNAR 447 ++ V+ I Y A +LV ++V + Sbjct: 434 ASYSFLVMGIAYLCATMLVWKFVERK 459 >E8VE14.1 E8VE14_BACST Toxic compound efflux transporter Length = 466 Score = 389 bits (998), Expect = e-106, Method: Compositional matrix adjust. Identities = 213/447 (47%), Positives = 297/447 (66%), Gaps = 1/447 (0%) Query: 3 AQVGLLPLVTLAIGFVMAMLDVTVVNVGLSSIEKSLATPLSMLVWIVDGYTLSFAAMLLV 62 + G+ L+ LA+GF+MA LDVTVVNV ++ ++ +L+ LS + W+VDGY L+FA++LL Sbjct: 12 SSTGISVLIVLALGFLMATLDVTVVNVAMADMKSTLSMSLSGVTWVVDGYILTFASLLLA 71 Query: 63 GGALADRYGAKNIYLLGLVLFVVASLLCGAAPNGGMLVAARLLQGLGAAFFMPSSLSLLT 122 GGALADR+G+K IY+LGL +FV+AS LCGA+ NG ML+A RL+QG+GAA FMPSSLSLL Sbjct: 72 GGALADRFGSKTIYILGLAIFVLASCLCGASFNGQMLIAGRLIQGIGAALFMPSSLSLLA 131 Query: 123 HVYEDDRVRARMLGVWSATVGGAAAVGPLVGGILIHSFGWRSVFLINVPVGLLGLYMAVK 182 Y D+R RARM G+W+A V A+A+GP +GG+L+ GW+S+FLINVP+G L A + Sbjct: 132 ASYPDERARARMFGLWAALVSAASALGPFIGGVLVELAGWQSIFLINVPIGAAALISAYR 191 Query: 183 MIPSVERHVRALTIASHFLGVLMLAALSFVLIEGPVYGWTSLPIATAVVVTMLAFGTLVQ 242 ++ V + I H LG++ L LS+ LI+GP GW S I A +LAF + Sbjct: 192 ILSRVPGKSSRVNIIGHLLGMMALGFLSYALIQGPSAGWRSPVILVAFTAAILAFVLFLL 251 Query: 243 RERSGSAPLLPRALFATPQFAAANGIGFLINFGVYGQFFFLALFLQQARGADALQTGLQM 302 RE S AP+LP +L+ +F+AA IGFL+NF ++G F L+LFLQ+A GA + G+++ Sbjct: 252 REISAKAPILPASLYKNGRFSAAQFIGFLLNFALFGGMFMLSLFLQEAGGASSFMAGVEL 311 Query: 303 LPMMAVIFIGNLNSGRMTARFGPRLPLRLGLTVGAVFSAVQIGL-TPETPYWMLAVAGAL 361 LPMMAV IGNL R+ RF + + + V + + + L +P+ PYW LAV ++ Sbjct: 312 LPMMAVFVIGNLLFARLANRFEAGQLMFVSMAVSCIIALLLFVLISPDFPYWQLAVLMSV 371 Query: 362 ANLGISTAIPAMTTAVMQVAGKGHANSAAAALNANRQIGALVGVALMGAILHSFGNWITR 421 NL +PAMTT +MQ AG+ HAN A AALNANRQIGALVGVA+ G I+H W Sbjct: 372 MNLCTGITVPAMTTVIMQAAGQRHANIAGAALNANRQIGALVGVAITGVIIHLSATWYAG 431 Query: 422 IVLAYGVVAIVYALALVLVLRYVNARS 448 + ++ Y+LA +LV ++ A + Sbjct: 432 AGFTFLMMGASYSLAALLVWLFLAAHN 458