BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0205.p (454 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8J1S6.1 D8J1S6_HERSS Small-conductance mechanosensitive channel... 541 e-151 A6T161.1 A6T161_JANMA Mechanosensitive ion channel protein (MscS... 394 e-107 A4G7W4.1 A4G7W4_HERAR Putative mechanosensitive ion channel protein 372 e-101 Q63WH9.1 Q63WH9_BURPS Putative mechanosensitive ion channel protein 297 3e-78 B7CID0.1 B7CID0_BURPS Transporter, MscS family 297 3e-78 >D8J1S6.1 D8J1S6_HERSS Small-conductance mechanosensitive channel protein Length = 358 Score = 541 bits (1393), Expect = e-151, Method: Compositional matrix adjust. Identities = 265/356 (74%), Positives = 300/356 (84%), Gaps = 4/356 (1%) Query: 97 MMPLMISQVAMRFVFYVLRRIFVKETTAGAFLQLFEKVFATLVWICVLLYITGMWPDLVD 156 M PL ++QVAMRFVFYVLR IFVK+ T GA L L EK A LVW+CV+L+ITG+WPD VD Sbjct: 1 MFPLTVAQVAMRFVFYVLRGIFVKDATVGALLHLSEKAIAVLVWLCVVLWITGLWPDFVD 60 Query: 157 YLDTTMLPLGRHKTSLLTILQATASVLVTLIIALWAGAMLEDRMMKLNTMHLSLRTVVAR 216 YLD T LPL RHK SLLT+LQ ASVLVTLIIALW G +LE+R+MK + MH SLRTVVAR Sbjct: 61 YLDGTTLPLARHKVSLLTMLQGAASVLVTLIIALWIGTVLENRLMKFSGMHSSLRTVVAR 120 Query: 217 MVRAILILVAILVSLSAVGIDLTVLSVFGGALGVGLGLGLQKIASSYVSGFVILLERSLA 276 M+RA+LIL+A LVSLS VGIDLTVLSVFGGALGVG+GLGLQKIASSYVSGFVILLERS+ Sbjct: 121 MLRALLILIAFLVSLSLVGIDLTVLSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMV 180 Query: 277 IGDMVNVDRYFGKVTQINTRYTILEGLDGIESVLPNEMFMSTPVQNYSLNRKIIRLATQV 336 IGDMV VD+YFGKVTQINTRYTILEGLDGIESVLPNE+F+S PVQNYSL +I+RL+TQ+ Sbjct: 181 IGDMVAVDKYFGKVTQINTRYTILEGLDGIESVLPNELFVSNPVQNYSLTHRIVRLSTQL 240 Query: 337 TILYQDDIETVLSMLEKAAFAVERVSDQILPQALLLKIGEDGLQLEIGFWITDPENGRLN 396 TILYQDD+ETVLS++E+AA V+RVS Q PQALL+KIG DGL LE+GFWITDPENGRLN Sbjct: 241 TILYQDDVETVLSIMEQAALGVQRVSQQTAPQALLIKIGADGLDLELGFWITDPENGRLN 300 Query: 397 VLSDVNRAIWRVFKTHGIQVAHPKRDIRLMDPRSFEQSVDDMTSSAHKDAQSAYNA 452 VLSDVNRAIW FK HGI+VAH KRDIR+MD RSF QS T + D N+ Sbjct: 301 VLSDVNRAIWAAFKQHGIEVAHAKRDIRIMDERSFRQS----TGKENPDMPGGQNS 352 >A6T161.1 A6T161_JANMA Mechanosensitive ion channel protein (MscS family) Length = 454 Score = 394 bits (1011), Expect = e-107, Method: Compositional matrix adjust. Identities = 227/434 (52%), Positives = 316/434 (72%), Gaps = 11/434 (2%) Query: 1 MSTLLTDFYDYLFGELPPTELLRQLAIIALCVACGWGLSRWLRKLFTLSGGDEQSVVHIG 60 +STL TD L+ +L L QL + LC A WG++R +R L + ++ +G Sbjct: 6 LSTLWTD----LWTDLGIPGLRWQLIAMVLCFALAWGIARIIRNLLATQEPQQNALRRLG 61 Query: 61 VKSFVRVLSPLLILILLTLARLVLIRLK--LPVTLWHVMMPLMISQVAMRFVFYVLRRIF 118 V+SF RVL PLL +LL +A+ +L RL V + V+MPL+ S +R FYV+RR+F Sbjct: 62 VESFTRVLWPLLTWLLLAIAKAILERLDGVDGVDVLRVVMPLVASFALIRLAFYVMRRVF 121 Query: 119 VKETTAGAFLQLFEKVFATLVWICVLLYITGMWPDLVDYLDTTMLPLGRHKTSLLTILQA 178 + AF+ LFE+ FA+LVWI V L+ITG PD++++LD T +PLG+HK S++T+LQA Sbjct: 122 ARGGKTSAFVLLFERSFASLVWIVVALHITGFLPDIIEFLDDTFVPLGKHKVSMMTMLQA 181 Query: 179 TASVLVTLIIALWAGAMLEDRMMKLNTMHLSLRTVVARMVRAILILVAILVSLSAVGIDL 238 SV VTLIIALWA AM+E+R+M+L+ MH S+R V++RM RA+LIL+A+LVSLS VGIDL Sbjct: 182 VVSVGVTLIIALWASAMIEERLMRLDGMHSSMRAVLSRMSRAVLILIALLVSLSMVGIDL 241 Query: 239 TVLSVFGGALGVGLGLGLQKIASSYVSGFVILLERSLAIGDMVNVDRYFGKVTQINTRYT 298 TVLSVFGGALGVGLGLGLQKIASSYVSGF+ILLERS+ IGD V +D++ G+V QINTRYT Sbjct: 242 TVLSVFGGALGVGLGLGLQKIASSYVSGFIILLERSMTIGDTVTMDKFTGRVAQINTRYT 301 Query: 299 ILEGLDGIESVLPNEMFMSTPVQNYSLNRKIIRLATQVTILYQDDIETVLSMLEKAAFAV 358 +L G DG +++LPN++F+S VQN SL+ +++L T++++ Y+ DI+ L +LE+AA +V Sbjct: 302 VLRGGDGSDTILPNDLFVSNAVQNLSLSDTVVQLTTRISVDYRTDIDLALRLLEEAALSV 361 Query: 359 ERV---SDQILPQALLLKIGEDGLQLEIGFWITDPENGRLNVLSDVNRAIWRVFKTHGIQ 415 E+V D++ P A ++ G DG+ L++GFW+ D + GR VLSDVNRAIWR F+ + I Sbjct: 362 EKVRKGPDRV-PGATMMAFGADGIDLQLGFWVNDLD-GRGTVLSDVNRAIWRSFQANEIN 419 Query: 416 VAHPKRDIRLMDPR 429 V P+R+IRL+D + Sbjct: 420 VPFPQREIRLIDEQ 433 >A4G7W4.1 A4G7W4_HERAR Putative mechanosensitive ion channel protein Length = 451 Score = 372 bits (954), Expect = e-101, Method: Compositional matrix adjust. Identities = 218/427 (51%), Positives = 306/427 (71%), Gaps = 6/427 (1%) Query: 24 QLAIIALCVACGWGLSRWLRKLFTLSGGDEQSVVHIGVKSFVRVLSPLLILILLTLARLV 83 Q+ + LC + GW L+R + ++ G + F RV P L L+L+ +A+ V Sbjct: 25 QILALVLCFSVGWLLARLGSGVLAAHDAQRKNAPRPGAEKFARVWWPFLTLVLIGIAKPV 84 Query: 84 LIRLKLPVTLWHVMMPLMISQVAMRFVFYVLRRIFVKETTAGAFLQLFEKVFATLVWICV 143 L R + V L + +PL+ S V +R FY+LRR+F ++ +F+ LF + FA LVW V Sbjct: 85 LARW-ISVDLLRLALPLVASFVLVRLAFYILRRVFARDGKGRSFILLFGRGFAGLVWTIV 143 Query: 144 LLYITGMWPDLVDYLDTTMLPLGRHKTSLLTILQATASVLVTLIIALWAGAMLEDRMMKL 203 L+ITG+ PD+ +LD T++PLGR+K SL+T+LQA SV VTL++ALW A+ E+R+MKL Sbjct: 144 ALHITGVLPDIAQFLDDTLIPLGRNKVSLMTMLQAALSVGVTLLVALWLSALAEERLMKL 203 Query: 204 NTMHLSLRTVVARMVRAILILVAILVSLSAVGIDLTVLSVFGGALGVGLGLGLQKIASSY 263 N+MH S+R V++RM RA+LIL+A+L+SLS VGIDLTVLSVFGGALGVGLGLGLQK+ASSY Sbjct: 204 NSMHSSMRAVLSRMGRALLILIALLLSLSMVGIDLTVLSVFGGALGVGLGLGLQKLASSY 263 Query: 264 VSGFVILLERSLAIGDMVNVDRYFGKVTQINTRYTILEGLDGIESVLPNEMFMSTPVQNY 323 VSGFVILLERSLAIGD V VD++ G+V+QINTRYT+L G DG ++V+PNEMF+S+PVQN Sbjct: 264 VSGFVILLERSLAIGDTVIVDKFTGRVSQINTRYTVLRGGDGTDTVVPNEMFVSSPVQNL 323 Query: 324 SLNRKIIRLATQVTILYQDDIETVLSMLEKAAFAVERV--SDQILPQALLLKIGEDGLQL 381 +L ++++ T++T+ Y+ DI+ L +LE+AA +VERV P A+L+ G DG+ L Sbjct: 324 TLTDTLVQMTTRITVDYRTDIDLALRLLEEAALSVERVRPGPDRGPGAILVAFGADGIDL 383 Query: 382 EIGFWITDPENGRLNVLSDVNRAIWRVFKTHGIQVAHPKRDIRLMDP--RSFEQSVDDMT 439 ++GFW+ DPE GR VLS+VNRAIWR F+ H I V P+R+IRL+D R+ ++ ++ T Sbjct: 384 QLGFWVNDPE-GRGGVLSNVNRAIWRSFQAHDINVPFPQREIRLIDEQYRAVKEHLNTNT 442 Query: 440 SSAHKDA 446 + D+ Sbjct: 443 RRSTPDS 449 >Q63WH9.1 Q63WH9_BURPS Putative mechanosensitive ion channel protein Length = 450 Score = 297 bits (760), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 169/416 (40%), Positives = 252/416 (60%), Gaps = 8/416 (1%) Query: 13 FGELPPTELLRQLAIIALCVACGWGLSRWLRKLFTLSGGDEQSVVHIGVKSFVRVLSPLL 72 FG+ P + + +A +A W L+R LR G S R L PL+ Sbjct: 17 FGQ--PAMIWQAVAFFG-SLALAWLLARHLRGRIDARRRAAGRTPGPGALSLNRALFPLI 73 Query: 73 ILILLTLARLVLIRLKLPVTLWHVMMPLMISQVAMRFVFYVLRRIFVKETTAGAFLQLFE 132 + + LA L +P +L + + + + +F+V RR+F ++ A+L + E Sbjct: 74 GGVFMWLAETTFDEL-MPTSLLQLALVPLFGIGLIYILFFVARRVFARDGHTHAWLSIVE 132 Query: 133 KVFATLVWICVLLYITGMWPDLVDYLDTTMLPLGRHKTSLLTILQATASVLVTLIIALWA 192 K+ + +VWI + L + G+ D++ +LD+ + + +LL+I+ V VTL++A+W Sbjct: 133 KLVSIVVWIAMALTVLGIQRDVLGWLDSVQFRVANARLTLLSIVSGALWVCVTLVVAMWL 192 Query: 193 GAMLEDRMMKLNTMHLSLRTVVARMVRAILILVAILVSLSAVGIDLTVLSVFGGALGVGL 252 G++LE+R+M+ ++ +LR V+AR+ RA+LI A+L+ LS VGID+TVL VFGGALGVGL Sbjct: 193 GSVLEERLMRARSLDANLRVVLARVGRALLIFAAVLIGLSLVGIDVTVLGVFGGALGVGL 252 Query: 253 GLGLQKIASSYVSGFVILLERSLAIGDMVNVDRYFGKVTQINTRYTILEGLDGIESVLPN 312 G GLQKIAS+YVSGF+ILL+RSL +GD +NV G VTQI TRYT++ GLDG E+++PN Sbjct: 253 GFGLQKIASNYVSGFIILLDRSLRLGDAINVGGLQGVVTQIRTRYTVVRGLDGNETLIPN 312 Query: 313 EMFMSTPVQNYS--LNRKIIRLATQVTILYQDDIETVLSMLEKAAFAVERVSDQILPQAL 370 E ++ VQN S L R ++A QV Y D+E +++L AA VERV + P Sbjct: 313 EKLITDVVQNQSSYLTRGYAKVAVQVA--YTSDVERAMALLADAAKGVERVLEDPAPTPY 370 Query: 371 LLKIGEDGLQLEIGFWITDPENGRLNVLSDVNRAIWRVFKTHGIQVAHPKRDIRLM 426 L+ G DG+ LE+GFWI D G V S VNR IWR+F HGI + P+R++R++ Sbjct: 371 LVGFGADGIDLELGFWIADAAKGTAGVRSTVNRNIWRLFVAHGISIPFPQREVRVI 426 >B7CID0.1 B7CID0_BURPS Transporter, MscS family Length = 450 Score = 297 bits (760), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 169/416 (40%), Positives = 252/416 (60%), Gaps = 8/416 (1%) Query: 13 FGELPPTELLRQLAIIALCVACGWGLSRWLRKLFTLSGGDEQSVVHIGVKSFVRVLSPLL 72 FG+ P + + +A +A W L+R LR G S R L PL+ Sbjct: 17 FGQ--PAMIWQAVAFFG-SLALAWLLARHLRGRIDARRRAAGRTPGPGALSLNRALFPLI 73 Query: 73 ILILLTLARLVLIRLKLPVTLWHVMMPLMISQVAMRFVFYVLRRIFVKETTAGAFLQLFE 132 + + LA L +P +L + + + + +F+V RR+F ++ A+L + E Sbjct: 74 GGVFMWLAETTFDEL-MPTSLLQLALVPLFGIGLIYILFFVARRVFARDGHTHAWLSIVE 132 Query: 133 KVFATLVWICVLLYITGMWPDLVDYLDTTMLPLGRHKTSLLTILQATASVLVTLIIALWA 192 K+ + +VWI + L + G+ D++ +LD+ + + +LL+I+ V VTL++A+W Sbjct: 133 KLVSIVVWIAMALTVLGIQRDVLGWLDSVQFRVANARLTLLSIVSGALWVCVTLVVAMWL 192 Query: 193 GAMLEDRMMKLNTMHLSLRTVVARMVRAILILVAILVSLSAVGIDLTVLSVFGGALGVGL 252 G++LE+R+M+ ++ +LR V+AR+ RA+LI A+L+ LS VGID+TVL VFGGALGVGL Sbjct: 193 GSVLEERLMRARSLDANLRVVLARVGRALLIFAAVLIGLSLVGIDVTVLGVFGGALGVGL 252 Query: 253 GLGLQKIASSYVSGFVILLERSLAIGDMVNVDRYFGKVTQINTRYTILEGLDGIESVLPN 312 G GLQKIAS+YVSGF+ILL+RSL +GD +NV G VTQI TRYT++ GLDG E+++PN Sbjct: 253 GFGLQKIASNYVSGFIILLDRSLRLGDAINVGGLQGVVTQIRTRYTVVRGLDGNETLIPN 312 Query: 313 EMFMSTPVQNYS--LNRKIIRLATQVTILYQDDIETVLSMLEKAAFAVERVSDQILPQAL 370 E ++ VQN S L R ++A QV Y D+E +++L AA VERV + P Sbjct: 313 EKLITDVVQNQSSYLTRGYAKVAVQVA--YTSDVERAMALLADAAKGVERVLEDPAPTPY 370 Query: 371 LLKIGEDGLQLEIGFWITDPENGRLNVLSDVNRAIWRVFKTHGIQVAHPKRDIRLM 426 L+ G DG+ LE+GFWI D G V S VNR IWR+F HGI + P+R++R++ Sbjct: 371 LVGFGADGIDLELGFWIADAAKGTAGVRSTVNRNIWRLFVAHGISIPFPQREVRVI 426