BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0061.p (297 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8J0J8.1 D8J0J8_HERSS 3-hydroxyisobutyrate dehydrogenase protein... 498 e-139 B8LA10.1 B8LA10_9GAMM 3-hydroxyisobutyrate dehydrogenase EC=1.1.... 355 3e-96 B2FI84.1 B2FI84_STRMK Putative 3-hydroxyisobutyrate dehydrogenas... 353 2e-95 B4SSP6.1 B4SSP6_STRM5 3-hydroxyisobutyrate dehydrogenase EC=1.1.... 352 3e-95 Q8EGC2.1 Q8EGC2_SHEON 3-hydroxyisobutyrate dehydrogenase 350 2e-94 >D8J0J8.1 D8J0J8_HERSS 3-hydroxyisobutyrate dehydrogenase protein EC=1.1.1.31 Length = 297 Score = 498 bits (1281), Expect = e-139, Method: Compositional matrix adjust. Identities = 251/297 (84%), Positives = 275/297 (92%) Query: 1 MSADIVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAK 60 MSA+IVFIGLGNMGLPMAQNLVKAG+SVSGHDLVKASVDKLVE GG TEAD+MAAVAKA Sbjct: 1 MSANIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKAD 60 Query: 61 VVITMLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDA 120 VVITMLPAS+HVES+YLG+ G+LA+ KPGTLLIDCSTIAPE+AR VAAAA +G+ MLDA Sbjct: 61 VVITMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDA 120 Query: 121 PVSGGTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLG 180 PVSGGT GATAGTLTFMVGG+ + F A+PYL+KMGKAIYHAG SGSGQTVKVCNNMLLG Sbjct: 121 PVSGGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLG 180 Query: 181 ILMIGTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGF 240 ILMIGTSEAIRLG+ANGMDPKVLSE+MSKSSGRNWTLEVYNPCPGVME APASKGYAGGF Sbjct: 181 ILMIGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAGGF 240 Query: 241 GVDLMLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNMLAKAK 297 GVDLMLKDLGLAVENALAT ++P+GA ARNLYDIHS +G+G LDFSS+FNML KA+ Sbjct: 241 GVDLMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNMLGKAQ 297 >B8LA10.1 B8LA10_9GAMM 3-hydroxyisobutyrate dehydrogenase EC=1.1.1.31 Length = 296 Score = 355 bits (912), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 177/293 (60%), Positives = 224/293 (76%) Query: 5 IVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAKVVIT 64 I FIGLGNMG PMA NLVK G+SV DLV A+V V++G + A +A A+VVI+ Sbjct: 4 IAFIGLGNMGGPMAANLVKNGHSVRVFDLVPAAVQAAVDAGASAASTARDTLADAEVVIS 63 Query: 65 MLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDAPVSG 124 MLPAS+HVE +YLGD GILA I G L+IDCSTIAP SAR V+ AA +G M+DAPVSG Sbjct: 64 MLPASRHVEGVYLGDDGILAAIPAGALVIDCSTIAPASARKVSEAAAARGLQMIDAPVSG 123 Query: 125 GTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLGILMI 184 GT GA AGTLTF+VGG +D A+P L+ MGK I+H GASG+GQ K+CNNM LG++M Sbjct: 124 GTAGAQAGTLTFIVGGEEDALERARPVLQAMGKNIFHVGASGAGQVAKLCNNMALGVIMA 183 Query: 185 GTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGFGVDL 244 T EAI LG+A+G+DPKVLS++M+ S+GR+W EV NP PGV+ENAPAS+GY+GGFG DL Sbjct: 184 VTGEAIALGVAHGLDPKVLSQMMAVSTGRSWATEVCNPWPGVLENAPASRGYSGGFGSDL 243 Query: 245 MLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNMLAKAK 297 MLKD+GLAVE A++ G+S+P+G +ARNLY ++ ++G G LDFSSV ++ K Sbjct: 244 MLKDMGLAVEAAMSVGASIPLGEVARNLYSMNHQAGRGKLDFSSVVQLITSEK 296 >B2FI84.1 B2FI84_STRMK Putative 3-hydroxyisobutyrate dehydrogenase EC=1.1.1.31 Length = 296 Score = 353 bits (905), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 177/293 (60%), Positives = 224/293 (76%) Query: 5 IVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAKVVIT 64 I FIGLGNMG PMA NLVK G+SV DLV A+V V++G + A +A A+VVI+ Sbjct: 4 IAFIGLGNMGGPMAANLVKNGHSVRVFDLVPAAVQAAVDAGASAASSARDTLADAEVVIS 63 Query: 65 MLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDAPVSG 124 MLPAS+HVE +YLGD GILA I G L+IDCSTIAP SAR V+ AA +G M+DAPVSG Sbjct: 64 MLPASRHVEGVYLGDDGILAAIPAGALVIDCSTIAPASARKVSEAAAARGLQMIDAPVSG 123 Query: 125 GTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLGILMI 184 GT GA AGTLTF+VGG +D A+P L+ MGK I+H GASG+GQ K+CNNM LG++M Sbjct: 124 GTAGAQAGTLTFIVGGEEDALERARPVLQAMGKNIFHVGASGAGQVAKLCNNMALGVIMA 183 Query: 185 GTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGFGVDL 244 T EAI LG+A+G+DPKVLS++M+ S+GR+W EV NP PGV+ENAPAS+GY+GGFG DL Sbjct: 184 VTGEAIALGVAHGLDPKVLSQMMAVSTGRSWATEVCNPWPGVLENAPASRGYSGGFGSDL 243 Query: 245 MLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNMLAKAK 297 MLKD+GLAVE A++ G+S+P+G +ARNLY ++ ++G G LDFSSV ++ K Sbjct: 244 MLKDMGLAVEAAMSVGASIPLGEVARNLYSMNHQAGRGKLDFSSVVQLITSEK 296 >B4SSP6.1 B4SSP6_STRM5 3-hydroxyisobutyrate dehydrogenase EC=1.1.1.31 Length = 296 Score = 352 bits (904), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 177/293 (60%), Positives = 224/293 (76%) Query: 5 IVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAKVVIT 64 I FIGLGNMG PMA NLVK G++V DLV A+V V++G A A +A A+VVI+ Sbjct: 4 IAFIGLGNMGGPMAANLVKNGHTVRVFDLVPAAVQAAVDAGASAAASARETLADAEVVIS 63 Query: 65 MLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDAPVSG 124 MLPAS+HVE +YLGD GILA I G L+IDCSTIAP SAR V+ AA +G M+DAPVSG Sbjct: 64 MLPASRHVEGVYLGDDGILAAIPAGALVIDCSTIAPASARKVSEAAAARGLQMIDAPVSG 123 Query: 125 GTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLGILMI 184 GT GA AGTLTF+VGG +D A+P L+ MGK I+H GASG+GQ K+CNNM LG++M Sbjct: 124 GTAGAQAGTLTFIVGGEEDALERARPVLQAMGKNIFHVGASGAGQVAKLCNNMALGVIMA 183 Query: 185 GTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGFGVDL 244 T EAI LG+A+G+DPKVLS++M+ S+GR+W EV NP PGV+ENAPAS+GY+GGFG DL Sbjct: 184 VTGEAIALGVAHGLDPKVLSQMMAVSTGRSWATEVCNPWPGVLENAPASRGYSGGFGSDL 243 Query: 245 MLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNMLAKAK 297 MLKD+GLAVE A++ G+S+P+G +ARNLY ++ ++G G LDFSSV ++ K Sbjct: 244 MLKDMGLAVEAAMSVGASIPLGEVARNLYSMNHQAGRGKLDFSSVVQLITSEK 296 >Q8EGC2.1 Q8EGC2_SHEON 3-hydroxyisobutyrate dehydrogenase Length = 300 Score = 350 bits (898), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 175/296 (59%), Positives = 216/296 (72%), Gaps = 3/296 (1%) Query: 5 IVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAKVVIT 64 + FIGLGNMG PMA NL+KAG +V DLV ++ L E G + + A A A A VVIT Sbjct: 4 VAFIGLGNMGGPMAANLLKAGMTVRVFDLVHTAMQTLAEQGALVSSTACGAAAGANVVIT 63 Query: 65 MLPASKHVESIYLG---DKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDAP 121 MLPA KHV+++YLG +KG+L + TLLIDCSTI +SA++VA A + G +DAP Sbjct: 64 MLPAGKHVKNLYLGSGSEKGLLDVVASDTLLIDCSTIDAQSAQLVATQAAKNGLEFMDAP 123 Query: 122 VSGGTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLGI 181 VSGGT GA AGTLTF+ GG+D F A+P L MGK I+HAG G+GQ K+CNNMLL + Sbjct: 124 VSGGTSGAAAGTLTFICGGSDTAFERAQPVLNAMGKNIFHAGGPGAGQIAKICNNMLLSV 183 Query: 182 LMIGTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGFG 241 LM+GTSEA+++GI +G+DPKVLS +M SSG NWTLE YNPCPGVMEN P+SKGY GGF Sbjct: 184 LMVGTSEALQMGIDHGLDPKVLSNIMKVSSGGNWTLEKYNPCPGVMENVPSSKGYQGGFM 243 Query: 242 VDLMLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNMLAKAK 297 VDLM+KDLGL+ E AL + SS PMGALAR+LY H++ G+G DFSS+F A K Sbjct: 244 VDLMVKDLGLSFEAALLSNSSTPMGALARSLYVSHARQGNGHRDFSSIFEQFAPLK 299