BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0059.p (332 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8J0J9.1 D8J0J9_HERSS Methylmalonate-semialdehyde dehydrogenase ... 623 e-176 A9IIK7.1 A9IIK7_BORPD Methylmalonate-semialdehyde dehydrogenase ... 503 e-140 E8U067.1 E8U067_ALIDB Methylmalonate-semialdehyde dehydrogenase 503 e-140 E5UEZ1.1 E5UEZ1_ALCXX Methylmalonate-semialdehyde dehydrogenase 499 e-139 E3HTE8.1 E3HTE8_ACHXA Methylmalonate-semialdehyde dehydrogenase ... 498 e-139 >D8J0J9.1 D8J0J9_HERSS Methylmalonate-semialdehyde dehydrogenase protein EC=1.2.1.27 Length = 506 Score = 623 bits (1607), Expect = e-176, Method: Compositional matrix adjust. Identities = 298/320 (93%), Positives = 312/320 (97%) Query: 1 MSQANIPNVPLYINGEHVQSTSKEWRDVINPATQEVVARVPFATKEEVDRAVANAKEAFK 60 MSQANIPNVPLY+NGE VQSTSKEWRDV+NPATQEVVARVPFATKEEVDRAVANAKE FK Sbjct: 1 MSQANIPNVPLYLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFK 60 Query: 61 TWRNTSLAQRMRIMLKFQQLLRDNIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSI 120 TWRNTSLAQRMRIMLKFQQLLR+NIAPLAE+ITREHGKTLPDAEGEVMRGLEVVEHACSI Sbjct: 61 TWRNTSLAQRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSI 120 Query: 121 TSLQLGELAENVAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPISVACGNTFVLKPS 180 TSLQLGELAENVAGGVDVYT++QP+GVGAGITAFNFPVMLPCFMFPI+VACGNTFVLKPS Sbjct: 121 TSLQLGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPS 180 Query: 181 EQDPTSSLFLVELANKAGLPPGVLNVVHGGPEVANMICDHPDIKAVSFIGSTNVGTHIYR 240 EQDPTSSLFLVELAN+AGLPPGVLNVVHGGP+VANMICDHPDIKAVSFIGST+VGTHIYR Sbjct: 181 EQDPTSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYR 240 Query: 241 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGKTKE 300 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVG+T+E Sbjct: 241 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTRE 300 Query: 301 WIPEIVERSKARAKTPTSAR 320 WIPEIVERSKA P + R Sbjct: 301 WIPEIVERSKAMKVGPGTDR 320 >A9IIK7.1 A9IIK7_BORPD Methylmalonate-semialdehyde dehydrogenase EC=1.2.1.27 Length = 497 Score = 503 bits (1295), Expect = e-140, Method: Compositional matrix adjust. Identities = 237/308 (76%), Positives = 270/308 (87%) Query: 4 ANIPNVPLYINGEHVQSTSKEWRDVINPATQEVVARVPFATKEEVDRAVANAKEAFKTWR 63 + +P+VPL I G+ VQS + EWRDVI+PATQ+VVARVPFAT+EE+D AVANAKEAF+TWR Sbjct: 2 SEVPSVPLLIGGKLVQSKTTEWRDVIDPATQQVVARVPFATREELDLAVANAKEAFQTWR 61 Query: 64 NTSLAQRMRIMLKFQQLLRDNIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSL 123 NT RMR+MLK QQLLR+N A LAEMITREHGKTLPDAEGEV RGLEVVEHACSI +L Sbjct: 62 NTGQGARMRVMLKLQQLLRENTAKLAEMITREHGKTLPDAEGEVGRGLEVVEHACSIANL 121 Query: 124 QLGELAENVAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPISVACGNTFVLKPSEQD 183 QLGE AEN AGG+DVYT+ QP+GV AGITAFNFPVMLPCFMFPI+VACGNTFVLKPSEQD Sbjct: 122 QLGEYAENAAGGIDVYTLIQPLGVCAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQD 181 Query: 184 PTSSLFLVELANKAGLPPGVLNVVHGGPEVANMICDHPDIKAVSFIGSTNVGTHIYRRAS 243 PTSSLFL +LA +AGLPPGVLNVVHGGPE+AN +C+HPDIKAVSFIGST VG+ IY+RAS Sbjct: 182 PTSSLFLAQLALEAGLPPGVLNVVHGGPEIANGLCEHPDIKAVSFIGSTRVGSEIYQRAS 241 Query: 244 EAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGKTKEWIP 303 AGKR Q MMGAKNHC++LPDA + A+N LLGAAFGAAGQRCMA SV VLVG+ + W+P Sbjct: 242 NAGKRCQAMMGAKNHCVILPDADPEVALNQLLGAAFGAAGQRCMAASVAVLVGEARNWLP 301 Query: 304 EIVERSKA 311 + VER+K Sbjct: 302 DFVERAKG 309 >E8U067.1 E8U067_ALIDB Methylmalonate-semialdehyde dehydrogenase Length = 501 Score = 503 bits (1294), Expect = e-140, Method: Compositional matrix adjust. Identities = 233/309 (75%), Positives = 275/309 (88%) Query: 12 YINGEHVQSTSKEWRDVINPATQEVVARVPFATKEEVDRAVANAKEAFKTWRNTSLAQRM 71 +I G+ S S EWRDV NPATQEVVARVPFAT+EEV+ AVANA+EAF+TWR TSL+ RM Sbjct: 10 FIGGKPYPSKSAEWRDVTNPATQEVVARVPFATREEVELAVANAQEAFQTWRETSLSARM 69 Query: 72 RIMLKFQQLLRDNIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAEN 131 RIMLK Q L+R++ A +A++ITREHGKTLPDAEGEV RGLEVVEHACSIT+LQLGE+AEN Sbjct: 70 RIMLKLQHLIREHQAEIAQLITREHGKTLPDAEGEVGRGLEVVEHACSITTLQLGEIAEN 129 Query: 132 VAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPISVACGNTFVLKPSEQDPTSSLFLV 191 A GVDVY + QP+GVGAGITAFNFPVMLPCFMFP+++ACGNTFVLKPSEQDP+S++ LV Sbjct: 130 AATGVDVYNLLQPLGVGAGITAFNFPVMLPCFMFPMAIACGNTFVLKPSEQDPSSTMRLV 189 Query: 192 ELANKAGLPPGVLNVVHGGPEVANMICDHPDIKAVSFIGSTNVGTHIYRRASEAGKRAQC 251 ELA++AG+PPGVLNV+HGGPEVA+M+CDHPDIKA+SFIGST+VGTHIYRRASEAGKR Q Sbjct: 190 ELAHEAGVPPGVLNVIHGGPEVADMLCDHPDIKALSFIGSTHVGTHIYRRASEAGKRVQS 249 Query: 252 MMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGKTKEWIPEIVERSKA 311 MMGAKNHC+V+PDAPK+ A+NNLLG+AFGAAGQRCMANSV V VG ++W+PE+V +SKA Sbjct: 250 MMGAKNHCVVMPDAPKEHALNNLLGSAFGAAGQRCMANSVAVFVGAARDWLPELVAKSKA 309 Query: 312 RAKTPTSAR 320 P + R Sbjct: 310 MKVGPGTDR 318 >E5UEZ1.1 E5UEZ1_ALCXX Methylmalonate-semialdehyde dehydrogenase Length = 497 Score = 499 bits (1284), Expect = e-139, Method: Compositional matrix adjust. Identities = 235/307 (76%), Positives = 266/307 (86%) Query: 4 ANIPNVPLYINGEHVQSTSKEWRDVINPATQEVVARVPFATKEEVDRAVANAKEAFKTWR 63 + +P VPL I G+ VQS + EWRDVINPATQEVVA+VPFAT+ E+D A+AN+KEAFKTWR Sbjct: 2 SEVPRVPLLIGGKLVQSKTTEWRDVINPATQEVVAKVPFATRAELDEAIANSKEAFKTWR 61 Query: 64 NTSLAQRMRIMLKFQQLLRDNIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSL 123 N+ RMR+MLKFQ+LLR N A LAEMITREHGKTLPDAEGEV RGLEVVEHACSI +L Sbjct: 62 NSGQGLRMRVMLKFQELLRANTAKLAEMITREHGKTLPDAEGEVGRGLEVVEHACSIANL 121 Query: 124 QLGELAENVAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPISVACGNTFVLKPSEQD 183 QLGE AEN A G+DVYT+ QP+GV AGITAFNFPVMLPCFMFPI+VACGNTFVLKPSEQD Sbjct: 122 QLGEYAENAASGIDVYTLIQPLGVCAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQD 181 Query: 184 PTSSLFLVELANKAGLPPGVLNVVHGGPEVANMICDHPDIKAVSFIGSTNVGTHIYRRAS 243 PTSSLFL +LA +AGLPPGVLNVVHGGPE AN +C+HPDIKAVSFIGST VGT IY RAS Sbjct: 182 PTSSLFLAQLALEAGLPPGVLNVVHGGPETANGLCEHPDIKAVSFIGSTRVGTEIYNRAS 241 Query: 244 EAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGKTKEWIP 303 AGKR Q MMGAKNHC++LPDA + A+N LLGAAFGAAGQRCMA+SV VLVG+ + W+P Sbjct: 242 AAGKRCQSMMGAKNHCVILPDADPEVALNQLLGAAFGAAGQRCMASSVAVLVGEARNWLP 301 Query: 304 EIVERSK 310 + VER+K Sbjct: 302 DFVERAK 308 >E3HTE8.1 E3HTE8_ACHXA Methylmalonate-semialdehyde dehydrogenase (Acylating) 2 EC=1.2.1.27 Length = 497 Score = 498 bits (1281), Expect = e-139, Method: Compositional matrix adjust. Identities = 234/307 (76%), Positives = 266/307 (86%) Query: 4 ANIPNVPLYINGEHVQSTSKEWRDVINPATQEVVARVPFATKEEVDRAVANAKEAFKTWR 63 + +P VPL I G+ VQS + EWRDVINPATQEVVA+VPFAT+EE+D A++NAKEAFKTWR Sbjct: 2 SEVPRVPLLIGGKLVQSKTTEWRDVINPATQEVVAKVPFATREELDLAISNAKEAFKTWR 61 Query: 64 NTSLAQRMRIMLKFQQLLRDNIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSL 123 N+ RMR+MLKFQ+LLR N LAEMITREHGKTLPDAEGEV RGLEVVEHACSI +L Sbjct: 62 NSGQGARMRVMLKFQELLRANSGKLAEMITREHGKTLPDAEGEVGRGLEVVEHACSIANL 121 Query: 124 QLGELAENVAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPISVACGNTFVLKPSEQD 183 QLGE AEN A G+DVYT+ QP+GV AGITAFNFPVMLPCFMFPI+VACGNTF+LKPSEQD Sbjct: 122 QLGEYAENAASGIDVYTLIQPLGVCAGITAFNFPVMLPCFMFPIAVACGNTFILKPSEQD 181 Query: 184 PTSSLFLVELANKAGLPPGVLNVVHGGPEVANMICDHPDIKAVSFIGSTNVGTHIYRRAS 243 PTSSLFL +LA +AGLPPGVLNVVHGGPE AN +C+HPDIKAVSFIGST VGT IY RAS Sbjct: 182 PTSSLFLAQLALEAGLPPGVLNVVHGGPETANGLCEHPDIKAVSFIGSTRVGTEIYNRAS 241 Query: 244 EAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGKTKEWIP 303 AGKR Q MMGAKNHC++LPDA + A+N L+GAAFGAAGQRCMA+SV VLVG+ + W+P Sbjct: 242 AAGKRCQSMMGAKNHCVILPDADPEVALNQLVGAAFGAAGQRCMASSVAVLVGEARNWLP 301 Query: 304 EIVERSK 310 + VERSK Sbjct: 302 DFVERSK 308