BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0092.p (265 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IPE8.1 D8IPE8_HERSS Glutamate--cysteine ligase EC=6.3.2.2 426 e-117 A6SX59.1 GSH1_JANMA Glutamate--cysteine ligase EC=6.3.2.2 381 e-104 F2KAR1.1 F2KAR1_PSEBR Glutamate--cysteine ligase 372 e-101 Q1IGL1.1 GSH1_PSEE4 Glutamate--cysteine ligase EC=6.3.2.2 367 e-100 Q3KJQ5.1 GSH1_PSEPF Glutamate--cysteine ligase EC=6.3.2.2 362 3e-98 >D8IPE8.1 D8IPE8_HERSS Glutamate--cysteine ligase EC=6.3.2.2 Length = 531 Score = 426 bits (1096), Expect = e-117, Method: Compositional matrix adjust. Identities = 207/260 (79%), Positives = 227/260 (87%), Gaps = 3/260 (1%) Query: 1 MTDLLTRRLALFASPENRALLGRGLRGVERETLRVTPDGQLATTPHPAVLGSALTNEQIT 60 MTDLL RRL + P++ ALLG+GLRG+ERETLRV G LA TPHP LGSALTNE IT Sbjct: 1 MTDLLNRRLQQLSQPQHLALLGQGLRGIERETLRVDAQGHLARTPHPPALGSALTNELIT 60 Query: 61 TDYSESLLEFITPATHDIADALRRLDEIHRFVHANLNGELLWNESMPGVLPPEEDIPIAW 120 TDYSE+LLEFITPA DIADAL+RLDEIHRF +A L GE LWN SMP VLPPE++IPIAW Sbjct: 61 TDYSEALLEFITPAMPDIADALQRLDEIHRFAYAKLEGEFLWNASMPCVLPPEDEIPIAW 120 Query: 121 YGTSHIGTLKHVYRRGLALRYGKTMQCIAGIHYNFSLSEDVWKLLRELDDREQNVEPSFS 180 YG SHIG LKHVYRRGLALRYG+TMQCIAGIHYNFSLSED+W++LRE DD +V+ S S Sbjct: 121 YGNSHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLREGDD---SVDLSLS 177 Query: 181 ARDYQSESYIALIRNFRRYSWLLMYLFGASPALATNFLRGRPHQLETLSDDTLYLPYATS 240 RDYQSE YIALIRNFRRYSWLLMYLFGASPA+ATNFLRGRPHQLETLS+DTLYLPY TS Sbjct: 178 RRDYQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPHQLETLSEDTLYLPYGTS 237 Query: 241 LRMSDLGYQSNAQANITPHY 260 LRMSDLGYQ++AQANITPHY Sbjct: 238 LRMSDLGYQNHAQANITPHY 257 >A6SX59.1 GSH1_JANMA Glutamate--cysteine ligase EC=6.3.2.2 Length = 528 Score = 381 bits (979), Expect = e-104, Method: Compositional matrix adjust. Identities = 183/260 (70%), Positives = 213/260 (81%), Gaps = 6/260 (2%) Query: 1 MTDLLTRRLALFASPENRALLGRGLRGVERETLRVTPDGQLATTPHPAVLGSALTNEQIT 60 M++L TRRLAL A +R LL +G RG+ERETLRV G+LA PHP LGSALTN QIT Sbjct: 1 MSNLFTRRLALLADDAHRDLLNQGRRGIERETLRVDLQGKLALGPHPVALGSALTNPQIT 60 Query: 61 TDYSESLLEFITPATHDIADALRRLDEIHRFVHANLNGELLWNESMPGVLPPEEDIPIAW 120 TDYSESLLEFITPA HD+A AL LD IHRF +A L+ ELLW++SMP LP E DIPIAW Sbjct: 61 TDYSESLLEFITPAEHDVATALEELDRIHRFANAKLDHELLWSQSMPCTLPEEADIPIAW 120 Query: 121 YGTSHIGTLKHVYRRGLALRYGKTMQCIAGIHYNFSLSEDVWKLLRELDDREQNVEPSFS 180 YGTSHIG +KHVYRRGLALRYGK MQCIAG+HYN+SLSED+W++++ Q+ + Sbjct: 121 YGTSHIGMIKHVYRRGLALRYGKAMQCIAGLHYNYSLSEDLWRVIK------QSEQSRLD 174 Query: 181 ARDYQSESYIALIRNFRRYSWLLMYLFGASPALATNFLRGRPHQLETLSDDTLYLPYATS 240 + YQSESYI+LIRNF+RYSWLLMYLFGASPAL+T+FLRGR H+L+TLSDDTLYLPYATS Sbjct: 175 DKSYQSESYISLIRNFQRYSWLLMYLFGASPALSTHFLRGREHELQTLSDDTLYLPYATS 234 Query: 241 LRMSDLGYQSNAQANITPHY 260 LRMSDLGYQ+NAQA + P Y Sbjct: 235 LRMSDLGYQNNAQAGLMPPY 254 >F2KAR1.1 F2KAR1_PSEBR Glutamate--cysteine ligase Length = 532 Score = 372 bits (954), Expect = e-101, Method: Compositional matrix adjust. Identities = 186/260 (71%), Positives = 206/260 (79%), Gaps = 6/260 (2%) Query: 1 MTDLLTRRLALFASPENRALLGRGLRGVERETLRVTPDGQLATTPHPAVLGSALTNEQIT 60 ++DLL RRLAL N +LL + L G+ERE LRVT DG LA TPHP LGSALTNEQIT Sbjct: 6 LSDLLNRRLALLGERANLSLLEQCLHGIERECLRVTGDGHLAQTPHPKALGSALTNEQIT 65 Query: 61 TDYSESLLEFITPATHDIADALRRLDEIHRFVHANLNGELLWNESMPGVLPPEEDIPIAW 120 TDYSESLLEFITPA + AD LR LD+IHRF ++ L E LW+ SMP LP EEDIPIA+ Sbjct: 66 TDYSESLLEFITPALANPADTLRSLDKIHRFAYSKLGDEYLWSPSMPCPLPAEEDIPIAY 125 Query: 121 YGTSHIGTLKHVYRRGLALRYGKTMQCIAGIHYNFSLSEDVWKLLRELDDREQNVEPSFS 180 YGTS+IG LK+VYR+GLALRYGKTMQCIAGIHYNFSL E +W LLR+ + E S Sbjct: 126 YGTSNIGRLKYVYRKGLALRYGKTMQCIAGIHYNFSLPEKLWPLLRQAEGVEA------S 179 Query: 181 ARDYQSESYIALIRNFRRYSWLLMYLFGASPALATNFLRGRPHQLETLSDDTLYLPYATS 240 RD+QS +YIALIRNFRRYSWLLMYLFGASPAL FLRGRPHQLE L DTLYLPYATS Sbjct: 180 DRDFQSSAYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRPHQLEQLDPDTLYLPYATS 239 Query: 241 LRMSDLGYQSNAQANITPHY 260 LRMSDLGYQSNAQA +TP Y Sbjct: 240 LRMSDLGYQSNAQAGLTPCY 259 >Q1IGL1.1 GSH1_PSEE4 Glutamate--cysteine ligase EC=6.3.2.2 Length = 530 Score = 367 bits (942), Expect = e-100, Method: Compositional matrix adjust. Identities = 185/260 (71%), Positives = 205/260 (78%), Gaps = 8/260 (3%) Query: 1 MTDLLTRRLALFASPENRALLGRGLRGVERETLRVTPDGQLATTPHPAVLGSALTNEQIT 60 ++DLL RRL+L + N LL + L G+ERE LRVT DG+LA TPHP LGSALTNEQIT Sbjct: 6 LSDLLNRRLSLLGA--NLDLLKQCLHGIERECLRVTDDGRLAQTPHPEALGSALTNEQIT 63 Query: 61 TDYSESLLEFITPATHDIADALRRLDEIHRFVHANLNGELLWNESMPGVLPPEEDIPIAW 120 TDYSESLLEFITPA D A L L+EIHRFV+ L GE LW+ SMP LP EEDIPIA Sbjct: 64 TDYSESLLEFITPALADPAKVLDSLEEIHRFVYTKLGGEYLWSPSMPCALPAEEDIPIAE 123 Query: 121 YGTSHIGTLKHVYRRGLALRYGKTMQCIAGIHYNFSLSEDVWKLLRELDDREQNVEPSFS 180 YG+S+IG LKHVYR+GLALRYG+TMQCIAGIHYNFSL E +W LLR+ + EQN Sbjct: 124 YGSSNIGKLKHVYRKGLALRYGRTMQCIAGIHYNFSLPEALWPLLRDAEGGEQN------ 177 Query: 181 ARDYQSESYIALIRNFRRYSWLLMYLFGASPALATNFLRGRPHQLETLSDDTLYLPYATS 240 RDYQS +YIALIRNFRRYSWLLMYLFGASP L FLRGRPHQLE L + TLYLPYATS Sbjct: 178 DRDYQSSAYIALIRNFRRYSWLLMYLFGASPTLDKGFLRGRPHQLEELDEQTLYLPYATS 237 Query: 241 LRMSDLGYQSNAQANITPHY 260 LRMSDLGYQSNAQA +TP Y Sbjct: 238 LRMSDLGYQSNAQAGLTPCY 257 >Q3KJQ5.1 GSH1_PSEPF Glutamate--cysteine ligase EC=6.3.2.2 Length = 532 Score = 362 bits (930), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 182/260 (70%), Positives = 204/260 (78%), Gaps = 6/260 (2%) Query: 1 MTDLLTRRLALFASPENRALLGRGLRGVERETLRVTPDGQLATTPHPAVLGSALTNEQIT 60 +++LL RRLAL N +LL + L G+ERE LRVT +G+LA TPHP LGSALTNEQIT Sbjct: 6 LSELLNRRLALLGERANLSLLEQCLHGIERECLRVTGEGRLAQTPHPEALGSALTNEQIT 65 Query: 61 TDYSESLLEFITPATHDIADALRRLDEIHRFVHANLNGELLWNESMPGVLPPEEDIPIAW 120 TDYSESLLEFITPA D AD L LD+IHRF ++ L E LW+ SMP LP EEDIPIA+ Sbjct: 66 TDYSESLLEFITPALPDPADTLASLDKIHRFAYSKLGNEYLWSPSMPCPLPAEEDIPIAY 125 Query: 121 YGTSHIGTLKHVYRRGLALRYGKTMQCIAGIHYNFSLSEDVWKLLRELDDREQNVEPSFS 180 YGTS+IG LK+VYR+GLALRYGKTMQCIAGIHYNFSL E +W LL+E + + Sbjct: 126 YGTSNIGQLKYVYRKGLALRYGKTMQCIAGIHYNFSLPEKLWPLLKEAEGF------VGT 179 Query: 181 ARDYQSESYIALIRNFRRYSWLLMYLFGASPALATNFLRGRPHQLETLSDDTLYLPYATS 240 RD+QS SYIALIRNFRRYSWLLMYLFGASPAL FLRGR HQLE L DTLYLPYATS Sbjct: 180 DRDFQSSSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRAHQLEQLDPDTLYLPYATS 239 Query: 241 LRMSDLGYQSNAQANITPHY 260 LRMSDLGYQSNAQA +TP Y Sbjct: 240 LRMSDLGYQSNAQAGLTPCY 259