BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0085.p (321 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IPF0.1 D8IPF0_HERSS Dihydrolipoamide acetyltransferase compone... 575 e-162 A6SVR7.1 A6SVR7_JANMA Pyruvate dehydrogenase E2 component (Dihyd... 493 e-137 A4G354.1 A4G354_HERAR Dihydrolipoyllysine-residue acetyltransfer... 487 e-136 E6PWB1.1 E6PWB1_9ZZZZ Dihydrolipoyllysine-residue acetyltransfer... 454 e-126 F0Q378.1 F0Q378_ACIAP Pyruvate dehydrogenase complex dihydrolipo... 435 e-120 >D8IPF0.1 D8IPF0_HERSS Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (E2) protein EC=2.3.1.12 Length = 554 Score = 575 bits (1481), Expect = e-162, Method: Compositional matrix adjust. Identities = 279/314 (88%), Positives = 294/314 (93%), Gaps = 1/314 (0%) Query: 9 SSVNKSHASPSVRKYGRELGVDLTRVVGTGPKNRILQEDIQKFVKAALAGA-GTAASAPA 67 S+ K+HASPSVRK+ RELGVDL+RV TGPK RILQ D+Q FVK +AG+ AASAPA Sbjct: 241 STGGKAHASPSVRKFARELGVDLSRVPATGPKGRILQLDVQNFVKGVMAGSTSVAASAPA 300 Query: 68 KNGSGVGLDLLPWPSLDFSKFGTTELLPLSRIKKISGPNLHRNWVMIPHVTQFDEADVTE 127 NGSG G++LLPWPSLDFSKFG TEL PLSRIKKISGPNLHRNWVMIPHVTQFDEADVTE Sbjct: 301 TNGSGAGMNLLPWPSLDFSKFGETELQPLSRIKKISGPNLHRNWVMIPHVTQFDEADVTE 360 Query: 128 LEEFRKTSNDAYAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKQYYNI 187 LEEFRKTSNDA+AKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILK++YNI Sbjct: 361 LEEFRKTSNDAFAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKKFYNI 420 Query: 188 GFAADTPNGLVVPVIKNADQKTIGQIATEMGELSAQARDGKLKPADMQGATFTISSLGGI 247 GFAADTPNGLVVPVIKNADQK+IGQIA EMGELSAQARDGKLKPADMQGATFTISSLGGI Sbjct: 421 GFAADTPNGLVVPVIKNADQKSIGQIAIEMGELSAQARDGKLKPADMQGATFTISSLGGI 480 Query: 248 GGTAFTPIINAPEVAILGLSKSIIKPVWDGKQFAPRLMLPTSLSYDHRVVDGAMGARFSA 307 GGTAFTPIINAPEVAILGLSKSI KPVWDGKQF PRLMLPTSLSYDHRV+DGAMGARFSA Sbjct: 481 GGTAFTPIINAPEVAILGLSKSITKPVWDGKQFVPRLMLPTSLSYDHRVIDGAMGARFSA 540 Query: 308 YLAEVLGDLRKSLL 321 YLA+VLGDLRKSLL Sbjct: 541 YLADVLGDLRKSLL 554 >A6SVR7.1 A6SVR7_JANMA Pyruvate dehydrogenase E2 component (Dihydrolipoamide acetyltransferase) EC=2.3.1.12 Length = 458 Score = 493 bits (1268), Expect = e-137, Method: Compositional matrix adjust. Identities = 243/312 (77%), Positives = 271/312 (86%), Gaps = 4/312 (1%) Query: 12 NKSHASPSVRKYGRELGVDLTRVVGTGPKNRILQEDIQKFVKAALAGAGTAASAPA--KN 69 +K+HASPS+RK+ RELGVDL +V G+GPK RI QED+Q +VK +A AA+A A K+ Sbjct: 149 SKAHASPSIRKFARELGVDLGKVAGSGPKGRITQEDVQNYVKGIIAAGPVAATAAAPVKS 208 Query: 70 GSGVGLDLLPWPSLDFSKFGTTELLPLSRIKKISGPNLHRNWVMIPHVTQFDEADVTELE 129 G G GLDLLPWPSLDFSKFGTT L PLSRIKKISGPNLHRNWVMIPHVTQ+DEADVTELE Sbjct: 209 GGGAGLDLLPWPSLDFSKFGTTTLQPLSRIKKISGPNLHRNWVMIPHVTQYDEADVTELE 268 Query: 130 EFRKTSNDAYAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKQYYNIGF 189 E RK++N A AK S VKLTMLAFVIKA V+ALKK+P FNSSLDA GENLILKQYYNIGF Sbjct: 269 ELRKSTNTALAK--SGVKLTMLAFVIKACVAALKKYPEFNSSLDANGENLILKQYYNIGF 326 Query: 190 AADTPNGLVVPVIKNADQKTIGQIATEMGELSAQARDGKLKPADMQGATFTISSLGGIGG 249 AADTP GLVVPV+K DQKT+ QIA EMG+LSAQAR+GKLKPADMQGATFTISSLGGIGG Sbjct: 327 AADTPQGLVVPVVKGVDQKTVTQIAQEMGDLSAQAREGKLKPADMQGATFTISSLGGIGG 386 Query: 250 TAFTPIINAPEVAILGLSKSIIKPVWDGKQFAPRLMLPTSLSYDHRVVDGAMGARFSAYL 309 T FTP+INAPEVAI+GLSK+ +KPVWDGKQF PRL++P SLSYDHRV+DGA GARF YL Sbjct: 387 TYFTPLINAPEVAIIGLSKTSMKPVWDGKQFVPRLIMPLSLSYDHRVIDGAQGARFVTYL 446 Query: 310 AEVLGDLRKSLL 321 +EVL DLRKSLL Sbjct: 447 SEVLSDLRKSLL 458 >A4G354.1 A4G354_HERAR Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (E2) (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex) EC=2.3.1.12 Length = 455 Score = 487 bits (1254), Expect = e-136, Method: Compositional matrix adjust. Identities = 240/310 (77%), Positives = 264/310 (85%), Gaps = 8/310 (2%) Query: 17 SPSVRKYGRELGVDLTRVVGTGPKNRILQEDIQKFVKAALAGAGTAASAP-----AKNGS 71 SPS+RK+ RELGVDL V G+GPK RI Q+D+Q +VK ALA AG +SAP + Sbjct: 149 SPSIRKFARELGVDLGSVAGSGPKGRITQQDVQTYVKTALA-AGPVSSAPVAAPPGASRG 207 Query: 72 GVGLDLLPWPSLDFSKFGTTELLPLSRIKKISGPNLHRNWVMIPHVTQFDEADVTELEEF 131 G GLDLLPWPSLDFSKFG T L PLSRIKKISGPNLHRNWVMIPHVTQ+DEADVTELEE Sbjct: 208 GAGLDLLPWPSLDFSKFGETTLQPLSRIKKISGPNLHRNWVMIPHVTQYDEADVTELEEL 267 Query: 132 RKTSNDAYAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKQYYNIGFAA 191 RK++N A AK S VKLT+LAFVIKA V+ALKK+P FNSSLDA GENLILKQYYNIGFAA Sbjct: 268 RKSTNTALAK--SGVKLTILAFVIKACVAALKKYPEFNSSLDAAGENLILKQYYNIGFAA 325 Query: 192 DTPNGLVVPVIKNADQKTIGQIATEMGELSAQARDGKLKPADMQGATFTISSLGGIGGTA 251 DTP GLVVPV+K DQKT+ QIA EMGELSAQARDGKLKPADMQGATFTISSLGGIGGT Sbjct: 326 DTPQGLVVPVVKGVDQKTVTQIAQEMGELSAQARDGKLKPADMQGATFTISSLGGIGGTY 385 Query: 252 FTPIINAPEVAILGLSKSIIKPVWDGKQFAPRLMLPTSLSYDHRVVDGAMGARFSAYLAE 311 FTP+INAPEVAI+GLSK+ +KPVWDGKQF PRL++P SLSYDHRV+DGA GARF YL+E Sbjct: 386 FTPLINAPEVAIIGLSKTSMKPVWDGKQFVPRLIMPLSLSYDHRVIDGAQGARFVTYLSE 445 Query: 312 VLGDLRKSLL 321 VL DLRKSLL Sbjct: 446 VLTDLRKSLL 455 >E6PWB1.1 E6PWB1_9ZZZZ Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (E2) (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex) EC=2.3.1.12 Length = 473 Score = 454 bits (1168), Expect = e-126, Method: Compositional matrix adjust. Identities = 222/318 (69%), Positives = 257/318 (80%), Gaps = 7/318 (2%) Query: 9 SSVNKSHASPSVRKYGRELGVDLTRVVGTGPKNRILQEDIQKFVKAALAGAGTAASAPAK 68 +S HASP+VRK RELGV L V GTGPK RI ED+Q FVKA +AG T +A + Sbjct: 158 ASTQLPHASPTVRKLARELGVPLGEVKGTGPKGRISPEDVQAFVKAVMAGKATTQAAATQ 217 Query: 69 N-----GSGVGLDLLPWPSLDFSKFGTTELLPLSRIKKISGPNLHRNWVMIPHVTQFDEA 123 + G+G+GL+LLPWP +DF+KFG PLSRIKK+SGPNL RNW MIPHVTQFDEA Sbjct: 218 SVGKPAGNGLGLNLLPWPKVDFAKFGPIRTEPLSRIKKLSGPNLARNWAMIPHVTQFDEA 277 Query: 124 DVTELEEFRKTSNDAYAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKQ 183 D+TELEEFRK+SN+ AK VKLTMLAFV+KA V+ALK PAFN+SLD GENLILK Sbjct: 278 DITELEEFRKSSNERLAKQG--VKLTMLAFVMKACVTALKAMPAFNASLDESGENLILKD 335 Query: 184 YYNIGFAADTPNGLVVPVIKNADQKTIGQIATEMGELSAQARDGKLKPADMQGATFTISS 243 Y ++GFAADTP GLVVPV+K+ D+K GQIATEMGEL+A AR+GKLKP+DMQGATFT+SS Sbjct: 336 YVHLGFAADTPQGLVVPVLKDVDKKGFGQIATEMGELAATAREGKLKPSDMQGATFTLSS 395 Query: 244 LGGIGGTAFTPIINAPEVAILGLSKSIIKPVWDGKQFAPRLMLPTSLSYDHRVVDGAMGA 303 LGG+GGTAFTPIINAPEVAILGLSKS IKPVWDGK F PRLMLP SLSYDHRV+DGAM A Sbjct: 396 LGGVGGTAFTPIINAPEVAILGLSKSQIKPVWDGKAFQPRLMLPLSLSYDHRVIDGAMAA 455 Query: 304 RFSAYLAEVLGDLRKSLL 321 RF+ L E+L D+R++LL Sbjct: 456 RFTTLLGELLADMRRTLL 473 >F0Q378.1 F0Q378_ACIAP Pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase EC=2.3.1.12 Length = 565 Score = 435 bits (1118), Expect = e-120, Method: Compositional matrix adjust. Identities = 216/311 (69%), Positives = 247/311 (79%), Gaps = 8/311 (2%) Query: 15 HASPSVRKYGRELGVDLTRVVGTGPKNRILQEDIQKFVKAALAGA----GTAASAPAKNG 70 HASPSVRK+ RELGV L + G+GPK RI QED+Q F + +AG AA APA G Sbjct: 259 HASPSVRKFARELGVPLEEIKGSGPKGRITQEDVQAFTRQVMAGGVQTKAQAAKAPAGGG 318 Query: 71 SGVGLDLLPWPSLDFSKFGTTELLPLSRIKKISGPNLHRNWVMIPHVTQFDEADVTELEE 130 SGVG+DLLPWP +DFSKFG E LSRIKKISG NLHRNWVMIPHVT DEAD+TELE Sbjct: 319 SGVGMDLLPWPKVDFSKFGGVERKDLSRIKKISGANLHRNWVMIPHVTNNDEADITELEA 378 Query: 131 FRKTSNDAYAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKQYYNIGFA 190 FR ++N KS VK+TMLAFVIKA V+ALKKFP FN+SLD G+ L+ KQY++IGFA Sbjct: 379 FRVSTN--KENEKSGVKVTMLAFVIKAVVAALKKFPEFNTSLD--GDTLVYKQYFHIGFA 434 Query: 191 ADTPNGLVVPVIKNADQKTIGQIATEMGELSAQARDGKLKPADMQGATFTISSLGGIGGT 250 ADTPNGLVVPV+K+AD+K I QI+ EMGEL+ +ARDGKL ADMQG F+ISSLGGIGGT Sbjct: 435 ADTPNGLVVPVLKDADKKGILQISQEMGELAKKARDGKLGAADMQGGCFSISSLGGIGGT 494 Query: 251 AFTPIINAPEVAILGLSKSIIKPVWDGKQFAPRLMLPTSLSYDHRVVDGAMGARFSAYLA 310 FTPIINAPEVAILGLSK +KPVWDGKQF PRL+LP SLSYDHRV+DGA ARF+AYL Sbjct: 495 HFTPIINAPEVAILGLSKGQMKPVWDGKQFVPRLVLPLSLSYDHRVIDGASAARFNAYLG 554 Query: 311 EVLGDLRKSLL 321 +VL D R+ LL Sbjct: 555 QVLADYRRILL 565