BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0046.p (307 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8J1Z2.1 D8J1Z2_HERSS tRNA pseudouridine synthase A EC=5.4.99.12 419 e-115 A4G4G0.1 TRUA_HERAR tRNA pseudouridine synthase A EC=5.4.99.12 386 e-105 A6T009.1 TRUA_JANMA tRNA pseudouridine synthase A EC=5.4.99.12 385 e-105 C3XAU3.1 C3XAU3_OXAFO tRNA pseudouridine synthase A EC=5.4.99.12 358 6e-97 C3X4N6.1 C3X4N6_OXAFO tRNA pseudouridine synthase A EC=5.4.99.12 353 1e-95 >D8J1Z2.1 D8J1Z2_HERSS tRNA pseudouridine synthase A EC=5.4.99.12 Length = 300 Score = 419 bits (1076), Expect = e-115, Method: Compositional matrix adjust. Identities = 205/291 (70%), Positives = 233/291 (80%), Gaps = 17/291 (5%) Query: 34 ALHRVVLGIQYDGNSWQGWQTQPHGLTVQDKLEAALKKFTLTDIDTTCAGRTDSGVHALE 93 L RVVLG+QYDG SWQGWQTQPH TVQD+LEAAL++F+ I TTCAGRTDSGVHALE Sbjct: 10 GLRRVVLGVQYDGASWQGWQTQPHRQTVQDQLEAALQRFSTQPIATTCAGRTDSGVHALE 69 Query: 94 QVVHFDTVIARETASWVRGVNAFLPPSIAVRWACEVALEE----------ASGLNPGER- 142 Q+VHFDT IAR++ SWVRG NAFLPP+IAVRWACE+ + A+ L+ +R Sbjct: 70 QIVHFDTPIARDSFSWVRGTNAFLPPTIAVRWACELPYVDDAAIASAASQAAELHGADRA 129 Query: 143 ------EDGFHARFSATARTYHYVLYNHPVRSPLLAGKAGWTFRPLDVGLMQQGADHLIG 196 + GFHARFSA ARTYHYVLYNH VR+PLLAGKAGWTFRPLD M + A HLIG Sbjct: 130 GADSLSQFGFHARFSAIARTYHYVLYNHAVRTPLLAGKAGWTFRPLDADRMHRAAQHLIG 189 Query: 197 MHDFSSFRAVECQARSPVRTMESLQVRRHGDMIVFTLKANAFLHHMVRNIVGSLIYVGNG 256 HDF+SFRAVECQA+SPVR MES+ VRRHGDMIVFTLKANAFLHHMVRNIVGSL+ VG+G Sbjct: 190 EHDFTSFRAVECQAKSPVRKMESIAVRRHGDMIVFTLKANAFLHHMVRNIVGSLVVVGSG 249 Query: 257 RQPPQWIAEVLAQKDRGCAAPTFMPDGLYLAKVDYPARWQLPQEAAAWPWL 307 +Q P W+ +VLA +DR AAPTFMPDGLYLA+VDYP W LPQE+ AWPWL Sbjct: 250 KQEPDWMGQVLALRDRSRAAPTFMPDGLYLARVDYPDAWALPQESRAWPWL 300 >A4G4G0.1 TRUA_HERAR tRNA pseudouridine synthase A EC=5.4.99.12 Length = 275 Score = 386 bits (992), Expect = e-105, Method: Compositional matrix adjust. Identities = 181/263 (68%), Positives = 212/263 (80%), Gaps = 9/263 (3%) Query: 37 RVVLGIQYDGNSWQGWQTQPHGLTVQDKLEAALKKFTLTDIDTTCAGRTDSGVHALEQVV 96 R+VLG+QYDG WQGWQTQ +GLTVQDKLE AL KFT I T CAGRTD+GVHALEQVV Sbjct: 13 RIVLGVQYDGAPWQGWQTQLNGLTVQDKLEFALYKFTQQTITTACAGRTDAGVHALEQVV 72 Query: 97 HFDTVIARETASWVRGVNAFLPPSIAVRWACEVALEEASGLNPGEREDGFHARFSATART 156 HFDT + R+ SWVRGVN FLP SIAVRWA EVA + A + FHARFSA +RT Sbjct: 73 HFDTTLERDMQSWVRGVNTFLPSSIAVRWASEVAHDPA---------ENFHARFSARSRT 123 Query: 157 YHYVLYNHPVRSPLLAGKAGWTFRPLDVGLMQQGADHLIGMHDFSSFRAVECQARSPVRT 216 YHY+LYN+PVRSPLL GKAGW FR LD+ MQ+ A HL+G HDFS+FR+V+CQA+SPV+T Sbjct: 124 YHYMLYNNPVRSPLLEGKAGWVFRALDIAKMQEAAAHLLGQHDFSAFRSVQCQAKSPVKT 183 Query: 217 MESLQVRRHGDMIVFTLKANAFLHHMVRNIVGSLIYVGNGRQPPQWIAEVLAQKDRGCAA 276 M ++++ R GD+I+FT+ ANAFLHHMVRNIVGSLIYVGNG Q P W+ EVLA ++R AA Sbjct: 184 MHAIKIERRGDLIMFTVHANAFLHHMVRNIVGSLIYVGNGTQAPSWLKEVLAGQERKFAA 243 Query: 277 PTFMPDGLYLAKVDYPARWQLPQ 299 PTFMPDGLYLAK+DY +WQLPQ Sbjct: 244 PTFMPDGLYLAKIDYDPKWQLPQ 266 >A6T009.1 TRUA_JANMA tRNA pseudouridine synthase A EC=5.4.99.12 Length = 271 Score = 385 bits (990), Expect = e-105, Method: Compositional matrix adjust. Identities = 181/263 (68%), Positives = 212/263 (80%), Gaps = 7/263 (2%) Query: 37 RVVLGIQYDGNSWQGWQTQPHGLTVQDKLEAALKKFTLTDIDTTCAGRTDSGVHALEQVV 96 R+VLG+QYDG WQGWQTQ +GLTVQD+LE AL KFTL + T CAGRTD+GVH LEQVV Sbjct: 7 RIVLGVQYDGTPWQGWQTQLNGLTVQDRLEFALSKFTLQTVSTVCAGRTDAGVHGLEQVV 66 Query: 97 HFDTVIARETASWVRGVNAFLPPSIAVRWACEVALEEASGLNPGEREDGFHARFSATART 156 HFDT + R+ +SWVRGVN FLPPSIAVRWA EVA NP E +D FHARFSA +R Sbjct: 67 HFDTTLERDMSSWVRGVNTFLPPSIAVRWATEVAH------NPDE-QDNFHARFSARSRM 119 Query: 157 YHYVLYNHPVRSPLLAGKAGWTFRPLDVGLMQQGADHLIGMHDFSSFRAVECQARSPVRT 216 Y YVLYN+PVRSPLL GKAGW FR LDV M+ A HL+G HDFS+FR+V+CQA+SPV++ Sbjct: 120 YQYVLYNNPVRSPLLEGKAGWVFRKLDVERMRTAAQHLLGEHDFSTFRSVQCQAKSPVKS 179 Query: 217 MESLQVRRHGDMIVFTLKANAFLHHMVRNIVGSLIYVGNGRQPPQWIAEVLAQKDRGCAA 276 M S+++ + GD+I+FTL ANAFLHHMVRNIVGSLIYVGNG Q P W+ E+L +DR AA Sbjct: 180 MYSIKIEQRGDLIMFTLHANAFLHHMVRNIVGSLIYVGNGAQQPDWLKELLDGRDRKFAA 239 Query: 277 PTFMPDGLYLAKVDYPARWQLPQ 299 PTFMPDGLYLAK+DY +WQLPQ Sbjct: 240 PTFMPDGLYLAKIDYDPKWQLPQ 262 >C3XAU3.1 C3XAU3_OXAFO tRNA pseudouridine synthase A EC=5.4.99.12 Length = 261 Score = 358 bits (919), Expect = 6e-97, Method: Compositional matrix adjust. Identities = 165/271 (60%), Positives = 208/271 (76%), Gaps = 10/271 (3%) Query: 35 LHRVVLGIQYDGNSWQGWQTQPHGLTVQDKLEAALKKFTLTDIDTTCAGRTDSGVHALEQ 94 + R+ LGI+YDG +W+GWQTQP G+TVQD+L+ A+ +FT T I T CAGRTD+GVHA EQ Sbjct: 1 MKRIALGIEYDGTNWRGWQTQPDGMTVQDQLQEAIFRFTRTRISTMCAGRTDAGVHASEQ 60 Query: 95 VVHFDTVIARETASWVRGVNAFLPPSIAVRWACEVALEEASGLNPGEREDGFHARFSATA 154 VVH DT + R SWVRGVNA+LP SIAVRWACEV PG + DGFHAR SA A Sbjct: 61 VVHLDTELDRLPVSWVRGVNAYLPSSIAVRWACEV---------PGGK-DGFHARSSAMA 110 Query: 155 RTYHYVLYNHPVRSPLLAGKAGWTFRPLDVGLMQQGADHLIGMHDFSSFRAVECQARSPV 214 R Y Y++YN PVRSPL +AGW F+PLD+GLMQ+GA L+G HDFS+FRA ECQ+ +PV Sbjct: 111 RRYRYLIYNSPVRSPLWKNRAGWVFKPLDIGLMQKGAGLLLGEHDFSAFRAAECQSITPV 170 Query: 215 RTMESLQVRRHGDMIVFTLKANAFLHHMVRNIVGSLIYVGNGRQPPQWIAEVLAQKDRGC 274 +T++ + + + G++IVFT +ANAFLHHMVRNIVGSL+Y+G G PP+W+ ++L KDR Sbjct: 171 KTVKRIDIEKQGNLIVFTFEANAFLHHMVRNIVGSLVYIGKGNHPPEWMKQLLDAKDRSL 230 Query: 275 AAPTFMPDGLYLAKVDYPARWQLPQEAAAWP 305 AAPTFMPDGLYL++V Y A+W LPQ +P Sbjct: 231 AAPTFMPDGLYLSRVHYEAKWGLPQREDVFP 261 >C3X4N6.1 C3X4N6_OXAFO tRNA pseudouridine synthase A EC=5.4.99.12 Length = 264 Score = 353 bits (907), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 163/271 (60%), Positives = 206/271 (76%), Gaps = 10/271 (3%) Query: 35 LHRVVLGIQYDGNSWQGWQTQPHGLTVQDKLEAALKKFTLTDIDTTCAGRTDSGVHALEQ 94 + R+ LGI+YDG W+GWQTQP G+TVQD+LEAA+++FT D+ TTCAGRTD+GVHA EQ Sbjct: 4 MKRIALGIEYDGTGWRGWQTQPDGMTVQDQLEAAIRQFTRMDMATTCAGRTDAGVHATEQ 63 Query: 95 VVHFDTVIARETASWVRGVNAFLPPSIAVRWACEVALEEASGLNPGEREDGFHARFSATA 154 VVH DT + R SWVRG+N++LP SIAVRWACEV PG + DGFHAR SA A Sbjct: 64 VVHLDTGLERLPVSWVRGINSYLPSSIAVRWACEV---------PGGK-DGFHARSSAIA 113 Query: 155 RTYHYVLYNHPVRSPLLAGKAGWTFRPLDVGLMQQGADHLIGMHDFSSFRAVECQARSPV 214 R Y Y++YN PVRSPL +AGW FRPLD+ M++GA L+G HDFS+FRA ECQ+ +PV Sbjct: 114 RRYRYLVYNSPVRSPLWKNRAGWVFRPLDLEWMREGAGFLLGEHDFSAFRAAECQSITPV 173 Query: 215 RTMESLQVRRHGDMIVFTLKANAFLHHMVRNIVGSLIYVGNGRQPPQWIAEVLAQKDRGC 274 +TM + + + G++IVFT +ANAFLHHM+RNIVGSLI VG G +PP+W+ E+L +DR Sbjct: 174 KTMNGIGIEKQGNLIVFTFEANAFLHHMIRNIVGSLIVVGKGNRPPKWMKELLESRDRSQ 233 Query: 275 AAPTFMPDGLYLAKVDYPARWQLPQEAAAWP 305 AAPTFMPDGLYL++V Y +W LPQ +P Sbjct: 234 AAPTFMPDGLYLSRVFYEEKWGLPQREDVFP 264