BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0003.p (378 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8ITT1.1 D8ITT1_HERSS Periplasmic binding protein 541 e-152 Q13YX7.1 Q13YX7_BURXL Small-conductance mechanosensitive ion cha... 365 8e-99 B2T442.1 B2T442_BURPP MscS Mechanosensitive ion channel 360 2e-97 D5NC42.1 D5NC42_9BURK MscS Mechanosensitive ion channel 358 6e-97 E6V640.1 E6V640_VARPD MscS Mechanosensitive ion channel 358 7e-97 >D8ITT1.1 D8ITT1_HERSS Periplasmic binding protein Length = 374 Score = 541 bits (1393), Expect = e-152, Method: Compositional matrix adjust. Identities = 262/378 (69%), Positives = 317/378 (83%), Gaps = 7/378 (1%) Query: 1 MEIPYLKDLEPGPLQSGLIVAVAVVVAILLAMVVHRIGILLLRRLAHNRPFTTNATHIAF 60 +++ +L ++ GPL+SGLIV +VA+++A+ +HR+GI ++RRLA RPFTT AT + F Sbjct: 3 IDLDFLYNMNEGPLRSGLIVLGVALVAVVVAVFLHRVGIAVVRRLARGRPFTTTATEVTF 62 Query: 61 RASQVCVALFAVRVVLTAAPDDTPGLRSLSYISSVALIVALTWCVKKCIESVSITITQLN 120 AS+ CV LF +R+VLT APD+TPGL S+SY++S+ LI+ALTW + +C++SVS+T+ LN Sbjct: 63 SASRTCVILFMLRLVLTGAPDNTPGLTSISYLTSIGLILALTWFLIRCVKSVSLTVINLN 122 Query: 121 PYDVADNLKARRILTQTRVLTRSAYFIVGLLGLAFVLLTLPGARQFGASLLASAGVAGLV 180 PYDVADNLKARRILTQTRVL+RSAYFI+ +LGL+FVLLTLPGARQFGASLLASAGVAG+V Sbjct: 123 PYDVADNLKARRILTQTRVLSRSAYFIIAMLGLSFVLLTLPGARQFGASLLASAGVAGIV 182 Query: 181 AGIAARPVLGNFIAGLQIAFSQPIRIDDVLIVNGEWGRVEEITGTFVVVRIWDERRMIVP 240 AGIAA+PVLGNF AGLQIAFSQPIRIDDVLIV GEWGRVEEITGTFVVVRIWDERRMIVP Sbjct: 183 AGIAAKPVLGNFFAGLQIAFSQPIRIDDVLIVKGEWGRVEEITGTFVVVRIWDERRMIVP 242 Query: 241 LQWFIENPFENWTHTSSTILGTVFLWLDFKLPVAALRAEFERVCKAAPQWDGRVCVLQVT 300 LQWFIENPFENWTHTSSTILGTVFLWLDF +P A+R EFERVCK+ P WD RVCV+ VT Sbjct: 243 LQWFIENPFENWTHTSSTILGTVFLWLDFSVPTQAIRDEFERVCKSLPLWDKRVCVMHVT 302 Query: 301 DVNERAMQVRMLVSAQNSGNAFDLRCAIRESMIAFLAEHHPEALPRLRSAIDPVELTPST 360 D +ER MQVR+LVSA++SG+AFDLRC IRE MI F+A ++P ALPRLR+ ++ Sbjct: 303 DASERTMQVRLLVSAKDSGSAFDLRCQIREHMIGFIARNYPGALPRLRAEVEARN----- 357 Query: 361 LADAGVQDKILDGAPTQR 378 D D I+DG PTQ+ Sbjct: 358 --DPEAIDSIIDGPPTQK 373 >Q13YX7.1 Q13YX7_BURXL Small-conductance mechanosensitive ion channel Length = 407 Score = 365 bits (936), Expect = 8e-99, Method: Compositional matrix adjust. Identities = 181/352 (51%), Positives = 244/352 (69%), Gaps = 3/352 (0%) Query: 3 IPYLKDLE---PGPLQSGLIVAVAVVVAILLAMVVHRIGILLLRRLAHNRPFTTNATHIA 59 +P L DL PL + L + V+A+LLA+ +HRIG + R A P T+ Sbjct: 1 MPTLDDLSRIAEAPLHTWLGALIVSVIAVLLAVGIHRIGARMAARFARPYPLTSVVLRYI 60 Query: 60 FRASQVCVALFAVRVVLTAAPDDTPGLRSLSYISSVALIVALTWCVKKCIESVSITITQL 119 + S + + A+ V APD + L +++ LI A+TW + ++ I Q Sbjct: 61 DKPSLFVLVILALEAVWWEAPDTLTFITPLRDAAAIGLIGAITWLSVRSAAAIGEAIIQA 120 Query: 120 NPYDVADNLKARRILTQTRVLTRSAYFIVGLLGLAFVLLTLPGARQFGASLLASAGVAGL 179 +P D ADNL+ARRI TQ RVL RS F++ ++G+ L+T P RQ GASLLASAGVAGL Sbjct: 121 HPLDTADNLQARRIHTQARVLARSVMFVIVIVGVGGALMTFPNVRQIGASLLASAGVAGL 180 Query: 180 VAGIAARPVLGNFIAGLQIAFSQPIRIDDVLIVNGEWGRVEEITGTFVVVRIWDERRMIV 239 VAGIAARPVLGN IAGLQIA SQPIR+DDV+++ GEWGR+EEITGT+V VR+WD+RR+IV Sbjct: 181 VAGIAARPVLGNLIAGLQIALSQPIRLDDVVVIQGEWGRIEEITGTYVSVRLWDQRRLIV 240 Query: 240 PLQWFIENPFENWTHTSSTILGTVFLWLDFKLPVAALRAEFERVCKAAPQWDGRVCVLQV 299 PLQWFIENPF NWT +SS I+GTVFL++D+++P+A LR E R+ + AP+WD RV VLQV Sbjct: 241 PLQWFIENPFSNWTRSSSQIIGTVFLYVDYRMPLAPLREELARIVEDAPEWDRRVQVLQV 300 Query: 300 TDVNERAMQVRMLVSAQNSGNAFDLRCAIRESMIAFLAEHHPEALPRLRSAI 351 TD ER+MQ+R LVS+ +SG +DLRC +RE ++ F+ +H+P+ LPR R+ + Sbjct: 301 TDGTERSMQLRALVSSLDSGLNWDLRCRVREGLLDFIQKHYPQYLPRARAEV 352 >B2T442.1 B2T442_BURPP MscS Mechanosensitive ion channel Length = 407 Score = 360 bits (924), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 178/352 (50%), Positives = 244/352 (69%), Gaps = 3/352 (0%) Query: 3 IPYLKDLE---PGPLQSGLIVAVAVVVAILLAMVVHRIGILLLRRLAHNRPFTTNATHIA 59 +P L DL PL + + V+A++LA+ +H+IG ++ RLA P T+ Sbjct: 1 MPTLDDLSRMAEAPLHTWPGALIVSVIAMMLAVGIHQIGARIVTRLARPYPLTSVVLRYI 60 Query: 60 FRASQVCVALFAVRVVLTAAPDDTPGLRSLSYISSVALIVALTWCVKKCIESVSITITQL 119 + S + + A+ V APD + L +++ LI +TW + ++ I Q Sbjct: 61 DKPSLFVLVILALEAVWWEAPDTLTFITPLRDAAAITLIGGITWLSVRSAAAIGEAIIQA 120 Query: 120 NPYDVADNLKARRILTQTRVLTRSAYFIVGLLGLAFVLLTLPGARQFGASLLASAGVAGL 179 +P D ADNL+ARRI TQ RVL RS F++ ++G+ L+T P RQ GASLLASAGVAGL Sbjct: 121 HPLDTADNLQARRIHTQARVLARSVMFVIVIVGVGGALMTFPSVRQIGASLLASAGVAGL 180 Query: 180 VAGIAARPVLGNFIAGLQIAFSQPIRIDDVLIVNGEWGRVEEITGTFVVVRIWDERRMIV 239 VAGIAARPVLGN IAGLQIA SQPIR+DDV+++ GEWGR+EEITGT+V VR+WD+RR+IV Sbjct: 181 VAGIAARPVLGNLIAGLQIALSQPIRLDDVVVIQGEWGRIEEITGTYVSVRLWDQRRLIV 240 Query: 240 PLQWFIENPFENWTHTSSTILGTVFLWLDFKLPVAALRAEFERVCKAAPQWDGRVCVLQV 299 PLQWFIENPF NWT +SS I+GTVFL++D+++P+A LR E R+ + AP+WD RV VLQV Sbjct: 241 PLQWFIENPFSNWTRSSSQIIGTVFLYVDYRMPLAPLREELARIVEDAPEWDRRVQVLQV 300 Query: 300 TDVNERAMQVRMLVSAQNSGNAFDLRCAIRESMIAFLAEHHPEALPRLRSAI 351 TD ER+MQ+R LVS+ +SG +DLRC +RE ++ F+ +H+P+ LPR R+ + Sbjct: 301 TDGTERSMQLRALVSSLDSGLNWDLRCRVREGLLDFIQQHYPQYLPRARAEV 352 >D5NC42.1 D5NC42_9BURK MscS Mechanosensitive ion channel Length = 407 Score = 358 bits (920), Expect = 6e-97, Method: Compositional matrix adjust. Identities = 184/352 (52%), Positives = 245/352 (69%), Gaps = 3/352 (0%) Query: 3 IPYLKDLE---PGPLQSGLIVAVAVVVAILLAMVVHRIGILLLRRLAHNRPFTTNATHIA 59 +P L DL PL + L + V+A+LLA+ VHRIG + RLA P T+ Sbjct: 1 MPTLDDLSRIAEAPLHTWLGALIVSVIAVLLAVGVHRIGARMATRLARPYPLTSVVLRYI 60 Query: 60 FRASQVCVALFAVRVVLTAAPDDTPGLRSLSYISSVALIVALTWCVKKCIESVSITITQL 119 + S + + A+ V APD + L +++ALI A+TW + ++ I Q Sbjct: 61 DKPSLFVLVILALEAVWWEAPDTLTFITPLRDAAAIALIGAITWLSVRSAAAIGEAIIQA 120 Query: 120 NPYDVADNLKARRILTQTRVLTRSAYFIVGLLGLAFVLLTLPGARQFGASLLASAGVAGL 179 +P D ADNL+ARRI TQ RVL RS F++ ++G+ L+T P RQ GASLLASAGVAGL Sbjct: 121 HPLDTADNLQARRIHTQARVLARSVMFVIVIVGVGGALMTFPNVRQIGASLLASAGVAGL 180 Query: 180 VAGIAARPVLGNFIAGLQIAFSQPIRIDDVLIVNGEWGRVEEITGTFVVVRIWDERRMIV 239 VAGIAARPVLGN IAGLQIA SQPIR+DDV+++ GEWGR+EEITGT+V VR+WD+RR+IV Sbjct: 181 VAGIAARPVLGNLIAGLQIALSQPIRLDDVVVIQGEWGRIEEITGTYVSVRLWDQRRLIV 240 Query: 240 PLQWFIENPFENWTHTSSTILGTVFLWLDFKLPVAALRAEFERVCKAAPQWDGRVCVLQV 299 PLQWFIENPF NWT +SS I+GTVFL++D+++P+A LR E R+ + AP+WD RV VLQV Sbjct: 241 PLQWFIENPFSNWTRSSSQIIGTVFLYVDYRMPLAPLREELARIVEDAPEWDRRVQVLQV 300 Query: 300 TDVNERAMQVRMLVSAQNSGNAFDLRCAIRESMIAFLAEHHPEALPRLRSAI 351 TD ER+MQ+R LVS+ +SG +DLRC +RE ++ F+ H+P+ LPR R+ + Sbjct: 301 TDGTERSMQLRALVSSLDSGLNWDLRCRVREGLLDFIQRHYPQYLPRARAEV 352 >E6V640.1 E6V640_VARPD MscS Mechanosensitive ion channel Length = 361 Score = 358 bits (919), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 181/332 (54%), Positives = 245/332 (73%) Query: 22 VAVVVAILLAMVVHRIGILLLRRLAHNRPFTTNATHIAFRASQVCVALFAVRVVLTAAPD 81 +AV+VA+ LA++VHRIG L+LRR+ P A+++ + L A+ +V AAPD Sbjct: 15 LAVLVAVPLALLVHRIGGLVLRRITRPVPAVHAMVVNIQPAARLALPLLALLLVFQAAPD 74 Query: 82 DTPGLRSLSYISSVALIVALTWCVKKCIESVSITITQLNPYDVADNLKARRILTQTRVLT 141 D + ++ +++ + LI A TW + + I + + +P DVADNL+ARR+LTQTRVL Sbjct: 75 DLRFIANVRHLTGLLLIAATTWLLVRAISGFADGVLAQHPSDVADNLQARRVLTQTRVLA 134 Query: 142 RSAYFIVGLLGLAFVLLTLPGARQFGASLLASAGVAGLVAGIAARPVLGNFIAGLQIAFS 201 R+A +V + G A +L+T PGARQ GASLLASAGV G+VAG+AA+PV N IAGLQIA + Sbjct: 135 RTAMTVVMVAGGAMMLMTFPGARQVGASLLASAGVIGIVAGLAAKPVFSNLIAGLQIALA 194 Query: 202 QPIRIDDVLIVNGEWGRVEEITGTFVVVRIWDERRMIVPLQWFIENPFENWTHTSSTILG 261 QPIRIDDVL+V GEWGRVEEITGTFVV++IWDERR+I+PL +FIE PF+NWT S+ +LG Sbjct: 195 QPIRIDDVLVVEGEWGRVEEITGTFVVLKIWDERRLILPLSYFIEKPFQNWTRHSAQLLG 254 Query: 262 TVFLWLDFKLPVAALRAEFERVCKAAPQWDGRVCVLQVTDVNERAMQVRMLVSAQNSGNA 321 +VF++LD+ +P+A LRAE ER+ KAAP+WDGR L+VTD ER MQVR+L +A SG A Sbjct: 255 SVFIYLDYGMPLAPLRAEVERIVKAAPEWDGRFFNLRVTDTTERTMQVRVLCTAATSGLA 314 Query: 322 FDLRCAIRESMIAFLAEHHPEALPRLRSAIDP 353 FDLRC++RE +I F+ +P+ LP++R DP Sbjct: 315 FDLRCSVREGLIEFMQREYPQFLPKMRIEADP 346